Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,536,878 C→T 5.9% E144E (GAG→GAA) ‡ narW ← nitrate reductase 2 (NRZ), delta subunit (assembly subunit)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,536,8780CT 5.9% 537.1 / 12.7 167E144E (GAG→GAA) ‡narWnitrate reductase 2 (NRZ), delta subunit (assembly subunit)
Reads supporting (aligned to +/- strand):  ref base C (77/80);  new base T (3/7);  total (80/87)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 171 total aligned reads displayed.

TTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGCC  >  NC_000913/1536732‑1537027
                                                                                                                                                  |                                                                                                                                                     
ttCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTcg                                                                                                                                                    <  1:58893/150‑1 (MQ=255)
   aCCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGAGACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgt                                                                                                                                                 <  1:883344/150‑1 (MQ=255)
   aCCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgt                                                                                                                                                 <  2:1627370/150‑1 (MQ=255)
    ccTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgtt                                                                                                                                                >  2:867662/1‑150 (MQ=255)
      tGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgttgt                                                                                                                                              >  1:187167/1‑150 (MQ=255)
        ttCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGttt                                                                                                                                            <  2:64369/150‑1 (MQ=255)
        ttCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGttt                                                                                                                                            <  1:828772/150‑1 (MQ=255)
        ttCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGAGGCACCGTGGTACGCGTTGttt                                                                                                                                            <  1:170741/150‑1 (MQ=255)
         tCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGAAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGtttt                                                                                                                                           >  1:1639616/1‑150 (MQ=255)
          ccTCTTCCCACTCTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGAGGCACCGTGGTACGCGTTGtttga                                                                                                                                          <  2:1743877/150‑3 (MQ=255)
          ccTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTa                                                                                                                                          >  2:1894577/1‑150 (MQ=255)
           ctctTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGAGGCACCGTGGTACGCGTTGtttgat                                                                                                                                         <  1:13180/150‑4 (MQ=38)
           ctctTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTATTGTCACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGAGGCACCGTGGTACGCGTTGtttgat                                                                                                                                         <  1:1054942/150‑4 (MQ=38)
             ctTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGAGGCACCGTGGTACGCGTTGtttgatgc                                                                                                                                       <  1:485446/150‑6 (MQ=38)
               tCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGa                                                                                                                                     >  2:757537/1‑150 (MQ=255)
               tCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGa                                                                                                                                     >  2:673634/1‑150 (MQ=255)
                 ccACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGAcc                                                                                                                                   >  1:1242909/1‑150 (MQ=255)
                 ccACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGGTTTACGCGAcc                                                                                                                                   >  1:1463107/1‑150 (MQ=255)
                        gCATCAAGCGCCTGGCGGGTGTCATCGCGCTCATCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGc                                                                                                                            <  1:404518/150‑1 (MQ=255)
                         cATCAAGCGCCTGGCGGGAGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACACCGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCa                                                                                                                           >  1:429167/1‑150 (MQ=255)
                          aTCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTCTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAg                                                                                                                          <  1:170849/150‑1 (MQ=255)
                           tCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAgg                                                                                                                         >  1:2172957/1‑150 (MQ=255)
                            cAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAggg                                                                                                                        >  2:1020722/1‑150 (MQ=255)
                            cAAGCGCCTGGCGGGTGTCAGCGCGCTCTTCGCTGTTTCCTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAggg                                                                                                                        <  1:1413529/150‑1 (MQ=255)
                              aGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCGGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCg                                                                                                                      >  1:246725/1‑150 (MQ=255)
                              aGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCg                                                                                                                      >  2:1358683/1‑150 (MQ=255)
                                cgcCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAg                                                                                                                    >  1:1031404/1‑150 (MQ=255)
                                 gcCTGGCGTGTGACATCGCGATCTTCGCTTTTTAATTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCATCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAgg                                                                                                                   <  2:377078/150‑1 (MQ=255)
                                 gcCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAACCAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAgg                                                                                                                   <  1:1460625/150‑1 (MQ=255)
                                 gcCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAgg                                                                                                                   <  1:1830034/150‑1 (MQ=255)
                                 gcCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAgg                                                                                                                   >  2:901143/1‑150 (MQ=255)
                                  ccTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGa                                                                                                                  >  1:1797069/1‑150 (MQ=255)
                                   cTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGAt                                                                                                                 <  2:1603088/150‑1 (MQ=255)
                                        gggTGGCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGgcg                                                                                                            <  1:1309764/150‑1 (MQ=255)
                                         ggTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGACAAGCAGGGCGAGGATCGgcgc                                                                                                           >  1:175172/1‑150 (MQ=255)
                                          gtgtCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCa                                                                                                          <  1:983841/150‑1 (MQ=255)
                                          gtgtCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCa                                                                                                          <  2:1223497/150‑1 (MQ=255)
                                          gtgtCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGGCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACCACGCGTACCTCGGTGCCTCGCGGTGTTTTAAGCGACCGCCAAGCAGTGCGAGGATCGGCGCa                                                                                                          <  1:361743/150‑1 (MQ=255)
                                           tgtCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCaa                                                                                                         <  1:1383397/150‑1 (MQ=255)
                                            gtCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGAGCGGCGCAAc                                                                                                        <  1:1778203/150‑1 (MQ=255)
                                                tCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCCCGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTg                                                                                                    >  2:1631713/1‑150 (MQ=255)
                                                tCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTACCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCACAACATTg                                                                                                    <  2:428677/150‑1 (MQ=255)
                                                  gcgcTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAg                                                                                                  >  2:1697542/1‑150 (MQ=255)
                                                   cgcTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGc                                                                                                 >  2:425889/1‑150 (MQ=255)
                                                     ctctTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCaa                                                                                               >  2:87568/1‑150 (MQ=255)
                                                          cGCTGTATACTTGTTTTGTGACCCTGTCACTTGAAAGGGCGCTTCCCGCCAGTTGCAGCAGAGCATCAAACACCGCCTACCACGGTGCCTCGCGTTGTTTTATGCGACCGCCCAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCctt                                                                                          >  2:1071623/1‑150 (MQ=255)
                                                           gCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCcttc                                                                                         <  2:269585/150‑1 (MQ=255)
                                                             tGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCcttctt                                                                                       <  1:206574/150‑1 (MQ=255)
                                                             tGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCACGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCcttctt                                                                                       <  1:1478103/150‑1 (MQ=255)
                                                               tttACTTGGTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTc                                                                                     <  2:144869/150‑1 (MQ=255)
                                                                ttACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGACAAGCAGGGCGAGGATCGGCGCAACAATGAGCAATCCTTCTTTCg                                                                                    >  2:1939596/1‑150 (MQ=255)
                                                                 tACTTGTTTTGTGACACTGTCACTCGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGCTCGGCGCAACATTGCGCAATCCGTCTTTCGc                                                                                   >  2:631160/1‑150 (MQ=255)
                                                                  aCTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGcc                                                                                  <  1:1131566/150‑1 (MQ=255)
                                                                  aCTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGAGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGcc                                                                                  >  1:1791751/1‑150 (MQ=255)
                                                                     tGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGa                                                                               <  1:706456/150‑1 (MQ=255)
                                                                     tGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGa                                                                               >  2:608738/1‑150 (MQ=255)
                                                                         ttGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGt                                                                           <  1:1358683/150‑1 (MQ=255)
                                                                           gtgACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACAACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCCACATTGAGCAAACCTTCTTTCGCCGGATCGTcc                                                                         >  2:1101630/1‑149 (MQ=255)
                                                                             gACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGTCTGATCGTCAgg                                                                       >  2:1524979/1‑150 (MQ=255)
                                                                               cacTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCa                                                                     <  1:1100849/150‑1 (MQ=255)
                                                                                acTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAg                                                                    <  1:2143641/150‑1 (MQ=255)
                                                                                  tGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCCGGCAGTa                                                                  >  2:2002742/1‑150 (MQ=255)
                                                                                      aCTTGAAAGGGAGCTTCCCGCCAGTTGCGGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCt                                                              <  2:246725/150‑1 (MQ=255)
                                                                                      aCTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCt                                                              <  1:295660/150‑1 (MQ=255)
                                                                                      aCTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCt                                                              >  2:1445209/1‑150 (MQ=255)
                                                                                              gggAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCCCGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACt                                                      >  2:52353/1‑150 (MQ=255)
                                                                                              gggAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACt                                                      >  2:505398/1‑150 (MQ=255)
                                                                                                 aGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCa                                                   <  2:2093820/150‑1 (MQ=255)
                                                                                                  gCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCTGATCGTCAGGCAGTACGCTTAAATACTCCAGa                                                 <  2:1031404/150‑1 (MQ=255)
                                                                                                  gCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCCTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGCCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAg                                                  >  2:1925738/1‑150 (MQ=255)
                                                                                                   cTTCCCGCCAGTTGCATCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAAACCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGa                                                 <  1:847802/150‑1 (MQ=255)
                                                                                                   cTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGCCCGCCAAGAAGTGCGAGGATCGGCGCATCATTGAGCAATACTTCTTTCGCCAGATCGTCAGGCACTACGCTTAAATACTCCAGa                                                 >  2:1217111/1‑150 (MQ=255)
                                                                                                    ttCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGAt                                                >  1:508438/1‑150 (MQ=255)
                                                                                                        cGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAg                                            >  1:288980/1‑150 (MQ=255)
                                                                                                           cAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCgg                                         <  1:688896/150‑1 (MQ=255)
                                                                                                                gcagcaGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGAt                                    <  2:1084290/150‑1 (MQ=255)
                                                                                                                gcagcaGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGAt                                    <  1:867662/150‑1 (MQ=255)
                                                                                                                gcagcaGAGCATCAAACAACGCGGACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGAt                                    <  1:1639230/150‑1 (MQ=255)
                                                                                                                   gcagAGCATCAAACAACGAGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACTCTTAAATACTCCAGATACAGCGGTAGATAAt                                 >  2:2120557/1‑150 (MQ=255)
                                                                                                                         cttaaaaCAACGCGAGGCACCGTGGTACGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCa                           >  1:747002/5‑150 (MQ=38)
                                                                                                                         cATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCa                           <  2:1153616/150‑1 (MQ=255)
                                                                                                                          aTCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCATTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAg                          <  1:1894577/150‑1 (MQ=255)
                                                                                                                            aaaaCAACGCGAGGCACCGTGGTACGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTTAATACTCCAGATACAGCGGTAGATAATCGGGCAGtt                        >  2:1291716/2‑150 (MQ=255)
                                                                                                                             aaaCAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTc                       >  1:1014066/1‑150 (MQ=255)
                                                                                                                              aacaacGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTcc                      >  2:26274/1‑150 (MQ=255)
                                                                                                                              aacaacGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTcc                      >  2:538668/1‑150 (MQ=255)
                                                                                                                               acaacGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTccc                     <  2:1655301/150‑1 (MQ=255)
                                                                                                                               acaacGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTccc                     <  2:2151417/150‑1 (MQ=255)
                                                                                                                               acaacGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTccc                     <  2:953832/150‑1 (MQ=255)
                                                                                                                                 aacGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGa                   <  2:1687695/150‑1 (MQ=255)
                                                                                                                                  acGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGAc                  <  2:1966225/150‑1 (MQ=255)
                                                                                                                                   cgcgTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACa                 >  1:1256585/1‑150 (MQ=255)
                                                                                                                                     cgTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGTGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAAt               >  2:2202217/1‑150 (MQ=255)
                                                                                                                                         ccACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAg           >  1:201771/1‑150 (MQ=255)
                                                                                                                                          cACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGc          <  1:1696854/150‑1 (MQ=255)
                                                                                                                                           aCGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCt         <  1:1279255/150‑1 (MQ=255)
                                                                                                                                           aCGGTGCCTCGAGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCTACATTGAGCTATCCTTCTTTCGCCTGTTCGTCAGGCAGTCCGCTTAAATACTCCAGATTGCGCGGTAGATTAGCGGGCAGTTCCCGACTCTCCAgat         >  1:381335/1‑148 (MQ=255)
                                                                                                                                              gTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCa      <  2:1856606/150‑1 (MQ=255)
                                                                                                                                                  cTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGcc  >  1:1604459/1‑150 (MQ=255)
                                                                                                                                                  cTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGcc  <  1:554328/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGCC  >  NC_000913/1536732‑1537027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: