Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,536,879 T→A 5.5% E144V (GAG→GTG) ‡ narW ← nitrate reductase 2 (NRZ), delta subunit (assembly subunit)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,536,8790TA 5.5% 490.6 / 11.4 171E144V (GAG→GTG) ‡narWnitrate reductase 2 (NRZ), delta subunit (assembly subunit)
Reads supporting (aligned to +/- strand):  ref base T (79/82);  new base A (3/7);  total (82/89)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 173 total aligned reads displayed.

TTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGCCG  >  NC_000913/1536732‑1537028
                                                                                                                                                   |                                                                                                                                                     
ttCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTcg                                                                                                                                                     <  1:58893/150‑1 (MQ=255)
   aCCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGAGACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgt                                                                                                                                                  <  1:883344/150‑1 (MQ=255)
    ccTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgtt                                                                                                                                                 >  2:867662/1‑150 (MQ=255)
        ttCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGttt                                                                                                                                             <  1:828772/150‑1 (MQ=255)
         tCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGAAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGtttt                                                                                                                                            >  1:1639616/1‑150 (MQ=255)
          ccTCTTCCCACTCTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGAGGCACCGTGGTACGCGTTGtttga                                                                                                                                           <  2:1743877/150‑3 (MQ=255)
          ccTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGAGGCACCGTGGTACGCGTTGtttga                                                                                                                                           <  2:2154983/150‑3 (MQ=255)
           ctctTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGAGGCACCGTGGTACGCGTTGtttgat                                                                                                                                          <  1:13180/150‑4 (MQ=38)
           ctctTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTATTGTCACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGAGGCACCGTGGTACGCGTTGtttgat                                                                                                                                          <  1:1054942/150‑4 (MQ=38)
             ctTCCCACACTGCATCAAGCGCCTGGCGGTAGTCATCGCGCTCTTCTCTGTTTACTTGTATTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGAGTACCACGGTGCCTCGCGTTGTTTTAAGc                                                                                                                                        >  1:1474378/1‑150 (MQ=255)
             ctTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGAGGCACCGTGGTACGCGTTGtttgatgc                                                                                                                                        <  1:485446/150‑6 (MQ=38)
               tCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGa                                                                                                                                      >  2:673634/1‑150 (MQ=255)
               tCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGa                                                                                                                                      >  2:757537/1‑150 (MQ=255)
                cccACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGAc                                                                                                                                     >  2:295660/1‑150 (MQ=255)
                 ccACACTGCATCAAGCGCCTGGCGGGTGTCATCGCTCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGAcc                                                                                                                                    >  2:1630405/1‑150 (MQ=255)
                 ccACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGAcc                                                                                                                                    >  1:1242909/1‑150 (MQ=255)
                 ccACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGGTTTACGCGAcc                                                                                                                                    >  1:1463107/1‑150 (MQ=255)
                      cTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCaa                                                                                                                               >  1:1856606/1‑150 (MQ=255)
                       tGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAg                                                                                                                              <  1:2115663/150‑1 (MQ=255)
                        gCATCAAGCGCCTGGCGGGTGTCATCGCGCTCATCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGc                                                                                                                             <  1:404518/150‑1 (MQ=255)
                         cATCAAGCGCCTGGCGGGAGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACACCGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCa                                                                                                                            >  1:429167/1‑150 (MQ=255)
                          aTCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTCTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAg                                                                                                                           <  1:170849/150‑1 (MQ=255)
                           tCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAgg                                                                                                                          >  1:2172957/1‑150 (MQ=255)
                            cAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAggg                                                                                                                         >  2:1020722/1‑150 (MQ=255)
                              aGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCGGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCg                                                                                                                       >  1:246725/1‑150 (MQ=255)
                              aGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCg                                                                                                                       >  2:1358683/1‑150 (MQ=255)
                                cgcCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAg                                                                                                                     >  1:1031404/1‑150 (MQ=255)
                                 gcCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAgg                                                                                                                    >  2:901143/1‑150 (MQ=255)
                                 gcCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAgg                                                                                                                    <  1:1646900/150‑1 (MQ=255)
                                  ccTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGa                                                                                                                   >  1:1797069/1‑150 (MQ=255)
                                   cTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGAt                                                                                                                  <  2:1603088/150‑1 (MQ=255)
                                        gggTGGCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGgcg                                                                                                             <  1:1309764/150‑1 (MQ=255)
                                         ggTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGACAAGCAGGGCGAGGATCGgcgc                                                                                                            >  1:175172/1‑150 (MQ=255)
                                          gtgtCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCa                                                                                                           <  1:983841/150‑1 (MQ=255)
                                          gtgtCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGGCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACCACGCGTACCTCGGTGCCTCGCGGTGTTTTAAGCGACCGCCAAGCAGTGCGAGGATCGGCGCa                                                                                                           <  1:361743/150‑1 (MQ=255)
                                           tgtCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCaa                                                                                                          <  1:1383397/150‑1 (MQ=255)
                                            gtCATCGCGCTCTTCGCTGATTACTTGTTTTGTGACACTGTCACTTGACAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAAc                                                                                                         <  1:1771486/150‑1 (MQ=255)
                                            gtCATCGCGCGCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAAc                                                                                                         <  1:1787053/150‑1 (MQ=255)
                                                tCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCCCGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTg                                                                                                     >  2:1631713/1‑150 (MQ=255)
                                                   cgcTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGc                                                                                                  <  2:2004869/150‑1 (MQ=255)
                                                   cgcTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGc                                                                                                  >  2:425889/1‑150 (MQ=255)
                                                     ctctTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCaa                                                                                                >  2:87568/1‑150 (MQ=255)
                                                          cGCTGTATACTTGTTTTGTGACCCTGTCACTTGAAAGGGCGCTTCCCGCCAGTTGCAGCAGAGCATCAAACACCGCCTACCACGGTGCCTCGCGTTGTTTTATGCGACCGCCCAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCctt                                                                                           >  2:1071623/1‑150 (MQ=255)
                                                               tttACTTGGTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTc                                                                                      <  2:144869/150‑1 (MQ=255)
                                                                ttACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGACAAGCAGGGCGAGGATCGGCGCAACAATGAGCAATCCTTCTTTCg                                                                                     >  2:1939596/1‑150 (MQ=255)
                                                                 tACTTGTTTTGTGACACTGTCACTCGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGCTCGGCGCAACATTGCGCAATCCGTCTTTCGc                                                                                    >  2:631160/1‑150 (MQ=255)
                                                                  aCTTGTTTTGTGACACTTGCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGcc                                                                                   <  1:1397840/150‑1 (MQ=255)
                                                                  aCTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGcc                                                                                   <  1:1131566/150‑1 (MQ=255)
                                                                  aCTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGcc                                                                                   <  2:1286907/150‑1 (MQ=255)
                                                                     tGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGa                                                                                >  2:608738/1‑150 (MQ=255)
                                                                     tGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGa                                                                                <  1:706456/150‑1 (MQ=255)
                                                                         ttGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGt                                                                            <  1:1358683/150‑1 (MQ=255)
                                                                           gtgACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACAACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCCACATTGAGCAAACCTTCTTTCGCCGGATCGTcc                                                                          >  2:1101630/1‑149 (MQ=255)
                                                                             gACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGTCTGATCGTCAgg                                                                        >  2:1524979/1‑150 (MQ=255)
                                                                               cacTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCa                                                                      <  1:1100849/150‑1 (MQ=255)
                                                                                 cTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCCCGGTGCCTCGCGTTGTTTTAAGCGACCGCAAAGCAGGGCGAGGATCGGAGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGt                                                                    >  1:1611780/1‑150 (MQ=255)
                                                                                      aCTTGAAAGGGAGCTTCCCGCCAGTTGCGGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCt                                                               <  2:246725/150‑1 (MQ=255)
                                                                                      aCTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCt                                                               <  2:1917725/150‑1 (MQ=255)
                                                                                      aCTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCt                                                               <  1:295660/150‑1 (MQ=255)
                                                                                      aCTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCt                                                               >  2:1445209/1‑150 (MQ=255)
                                                                                        ttGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTa                                                             <  1:485967/150‑1 (MQ=255)
                                                                                         tGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTaa                                                            >  2:1696854/1‑150 (MQ=255)
                                                                                          gAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTaaa                                                           >  2:1928230/1‑150 (MQ=255)
                                                                                          gAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTaaa                                                           <  2:389660/150‑1 (MQ=255)
                                                                                          gAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTaaa                                                           <  1:1433171/150‑1 (MQ=255)
                                                                                          gAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTaaa                                                           <  1:2024438/150‑1 (MQ=255)
                                                                                            aaGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTCCCTCGCGTTGTTTTAAGCGCCCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGCTCGTCAGGCAGTACGCTtatata                                                         >  2:469295/1‑150 (MQ=255)
                                                                                              gggAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCCCGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACt                                                       >  2:52353/1‑150 (MQ=255)
                                                                                              gggAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACt                                                       <  2:57156/150‑1 (MQ=255)
                                                                                                gAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTcc                                                     <  2:222196/150‑1 (MQ=255)
                                                                                                 aGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCa                                                    <  2:2093820/150‑1 (MQ=255)
                                                                                                  gCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCTGATCGTCAGGCAGTACGCTTAAATACTCCAGa                                                  <  2:1031404/150‑1 (MQ=255)
                                                                                                   cTTCCCGCCAGTTGCATCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAAACCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGa                                                  <  1:847802/150‑1 (MQ=255)
                                                                                                   cTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGCCCGCCAAGAAGTGCGAGGATCGGCGCATCATTGAGCAATACTTCTTTCGCCAGATCGTCAGGCACTACGCTTAAATACTCCAGa                                                  >  2:1217111/1‑150 (MQ=255)
                                                                                                     tCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATa                                                >  2:266839/1‑150 (MQ=255)
                                                                                                     tCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATa                                                <  2:1727786/150‑1 (MQ=255)
                                                                                                         gCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATAGAGCAATCCTTCATTCGCCAGATCGTCAGCCACTACGCTTAAATAATCCAGATACAGc                                            >  1:263623/1‑150 (MQ=255)
                                                                                                           cAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCgg                                          <  1:688896/150‑1 (MQ=255)
                                                                                                                gcagcaGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGAt                                     <  1:867662/150‑1 (MQ=255)
                                                                                                                gcagcaGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGAt                                     <  2:1084290/150‑1 (MQ=255)
                                                                                                                   gcagAGCATCAAACAACGAGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACTCTTAAATACTCCAGATACAGCGGTAGATAAt                                  >  2:2120557/1‑150 (MQ=255)
                                                                                                                        gCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGc                             <  1:1820437/150‑1 (MQ=255)
                                                                                                                         cttaaaaCAACGCGAGGCACCGTGGTACGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCa                            >  1:747002/5‑150 (MQ=38)
                                                                                                                         cATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCa                            <  2:1153616/150‑1 (MQ=255)
                                                                                                                          aTCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCATTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAg                           <  1:1894577/150‑1 (MQ=255)
                                                                                                                          aTCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAg                           >  2:197802/1‑150 (MQ=255)
                                                                                                                            cAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGtt                         >  1:1304883/1‑150 (MQ=255)
                                                                                                                            aaaaCAACGCGAGGCACCGTGGTACGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTTAATACTCCAGATACAGCGGTAGATAATCGGGCAGtt                         >  2:1291716/2‑150 (MQ=255)
                                                                                                                             aaaCAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTc                        >  1:1014066/1‑150 (MQ=255)
                                                                                                                               acaacGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTccc                      <  2:1655301/150‑1 (MQ=255)
                                                                                                                               acaacGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTccc                      <  2:953832/150‑1 (MQ=255)
                                                                                                                                  acGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGAc                   <  2:508438/150‑1 (MQ=255)
                                                                                                                                  acGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGAc                   <  1:169827/150‑1 (MQ=255)
                                                                                                                                   cgcgTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACa                  >  1:1256585/1‑150 (MQ=255)
                                                                                                                                     cgTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAAt                >  2:636564/1‑150 (MQ=255)
                                                                                                                                         ccACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAg            >  1:201771/1‑150 (MQ=255)
                                                                                                                                           aCGGTGCCTCGAGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCTACATTGAGCTATCCTTCTTTCGCCTGTTCGTCAGGCAGTCCGCTTAAATACTCCAGATTGCGCGGTAGATTAGCGGGCAGTTCCCGACTCTCCAgat          >  1:381335/1‑148 (MQ=255)
                                                                                                                                              gTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCa       <  2:1856606/150‑1 (MQ=255)
                                                                                                                                                 ccTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCACTCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGc    >  1:1597187/1‑150 (MQ=255)
                                                                                                                                                  cTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGcc   <  1:554328/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGCCG  >  NC_000913/1536732‑1537028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: