Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4290TG26.4% 112.6 / 14.2 73intergenic (+70/‑89)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (10/43);  new base G (18/1);  total (28/45)
Fisher's exact test for biased strand distribution p-value = 4.96e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTG  >  NC_000913/422284‑422577
                                                                                                                                                 |                                                                                                                                                    
cgcgTGGATTGTTGTGCTGTTGATTGGCTGGTTGTTTAAACGCCGCCACGATCGTCAGCTGGCTTAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAATTTATCCGCCCCCGtcctc                                                                                                                                                  <  1:1409838/150‑1 (MQ=255)
cgcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTTAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctc                                                                                                                                                  <  2:210249/150‑1 (MQ=255)
cgcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctc                                                                                                                                                  >  2:1481680/1‑150 (MQ=255)
cgcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctc                                                                                                                                                  <  2:589015/150‑1 (MQ=255)
  cgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGgccgccg                                                                                                                                                >  2:349489/1‑149 (MQ=255)
        ttgttgTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCa                                                                                                                                          <  2:686521/150‑1 (MQ=255)
        ttgttgTGCGGTTGATTGGCTGGATGTTTAACCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTGCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCa                                                                                                                                          <  2:319273/150‑1 (MQ=255)
           ttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAat                                                                                                                                       <  1:1379599/150‑1 (MQ=255)
               ggtGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATcc                                                                                                                                   <  1:1548233/148‑1 (MQ=255)
               gCTGTTGATTGGCTGTATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATcc                                                                                                                                   <  2:943184/150‑1 (MQ=255)
                  gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGGCCCCAAATATcgtag                                                                                                                                >  1:875274/1‑146 (MQ=255)
                     gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCGGTCAgg                                                                                                                             >  2:1461766/1‑149 (MQ=255)
                     gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCtggaga                                                                                                                             >  2:221803/1‑145 (MQ=255)
                      aTTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGGCTCCCCCAAAAATCCTTCAgag                                                                                                                            >  1:1619291/1‑149 (MQ=255)
                       ttGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCGGCGGGCTCCTCCGCCCAAGATCCtgctgagg                                                                                                                           >  2:1539308/1‑143 (MQ=255)
                       ttGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCGCCAGTGTCCGTCAgagg                                                                                                                           >  1:1962029/1‑148 (MQ=255)
                       tgggCTGGAGGTTTAAACGCCGCCACGATCGTCAGCGGGCTGAAAACCAGTAAGCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatg                                                                                                                           <  1:367583/148‑1 (MQ=255)
                          gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGatgatg                                                                                                                        <  1:1415358/150‑1 (MQ=255)
                          gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCGGCCCGCAAGTATCGTTGAgatgggg                                                                                                                        >  1:988963/1‑147 (MQ=255)
                            tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCTGCGCGCCCACCTATCCTTCAGATgaggag                                                                                                                      >  1:817340/1‑150 (MQ=255)
                            tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAg                                                                                                                      <  1:216526/150‑1 (MQ=255)
                             ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGt                                                                                                                     <  1:1345875/150‑1 (MQ=255)
                             ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGGCCCCAAAGATCCGGCAGATGTAGAGt                                                                                                                     >  1:1254870/1‑150 (MQ=255)
                              gATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTg                                                                                                                    >  2:1615167/1‑150 (MQ=255)
                                 gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCTCACCCCACTTGCCTGCAGCTGATGAGTGGTc                                                                                                                 >  1:335391/1‑150 (MQ=255)
                                 gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATc                                                                                                                 <  1:976152/150‑1 (MQ=255)
                                 gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATCCTTCAGATGATGAGTGATc                                                                                                                 >  2:201492/1‑150 (MQ=255)
                                 gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCGCCGCCCCCAAATAACCTTCGGAGGTGGAGTGAtg                                                                                                                 >  1:1370905/1‑149 (MQ=255)
                                  tttAAACGCCGCCACGATCGTCAGCTGTCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCTGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATcc                                                                                                                <  1:1443220/150‑1 (MQ=255)
                                   ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCTCCTCCTCCCCCAAATATACTTAAGATGATGAGTGTTccg                                                                                                               >  2:104219/1‑149 (MQ=255)
                                      aaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCGGCCACCAAAAATCGTTGAGATGATGAGTGAGCCGGCa                                                                                                            >  2:843796/1‑150 (MQ=255)
                                                cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTGTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTTTCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                  <  1:1525088/150‑1 (MQ=255)
                                                  aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGc                                                                                                <  2:1998386/150‑1 (MQ=255)
                                                   tCGTCAGCTGGCGTAAAACATTTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCAGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCa                                                                                               <  1:1011401/150‑1 (MQ=255)
                                                    cGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCTCCGCCTCCCCCCAATTTCCTTCTGATGATGTGTGATCCTGCATTAGGCTATGGcta                                                                                              >  2:1717989/1‑148 (MQ=255)
                                                         gctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATAGCCGACAGATCATGAGTGAGGCAGCATTAGGAGATGGCAAGGTgg                                                                                         >  2:1829581/1‑149 (MQ=255)
                                                           gggctgAACACCAGTAATCCTTGCGTTCTGTACGCCGGTTCAGGAGGAAATCCTGATCCGGCTAATCCGAAAGATATCCGCCCCCGGCCTCCTCCCCCAAATATCCTACAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATc                                                                                       <  2:135773/149‑1 (MQ=255)
                                                            ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCCGAAGTCCTTCAGATGATGAGTGATGCTGCATTTGGATATGGCAGGGTGTTCa                                                                                      >  1:593479/1‑150 (MQ=255)
                                                                      cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTTTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatc                                                                            <  1:1481409/150‑1 (MQ=255)
                                                                      cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGTCCTGCTCCGGCCATGATCCTACAGATGGTGAGTGATAGTGGAGTAGTCATAGGAAAGGTCATGAGAttttaaga                                                                            >  2:1834604/1‑146 (MQ=255)
                                                                      cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatc                                                                            <  2:1051078/150‑1 (MQ=255)
                                                                                  cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCACATGTCCGTGAGAGGATGAGGGAGCCGGCAGTAGGGGATGGCCGGGGGATCCGATTTTCATCAGAGGGGAATTa                                                                >  2:1393020/1‑150 (MQ=255)
                                                                                  cGTTCTGTAAGCCGGTGCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTa                                                                <  2:1189150/150‑1 (MQ=255)
                                                                                   gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCGGCCTGCCCCAAATATCCATAAGATTATAAATTATACAGCATTAGGCTATTGAAAGTTGATCAGATTTTAATGACAGTTGAAatat                                                               >  2:1267235/1‑150 (MQ=255)
                                                                                      cTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCATCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                            <  1:118627/150‑1 (MQ=255)
                                                                                      cTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAAATCCTTCAGATGATGGGTGATCCGGCAGTAGGCGATGGCAAGGTGTTGAGATTTTCATCACAGGGGAATTatgat                                                            >  2:1599150/1‑150 (MQ=255)
                                                                                       tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCCAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCGTTGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgatt                                                           >  2:717924/1‑149 (MQ=255)
                                                                                          aaGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTa                                                        <  1:1047490/150‑1 (MQ=255)
                                                                                          aaGCCGGTGCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCAAAATATCCTGCAGATGATGAGTGATCATGCATTAGGCTATGGCAAGGTGTTCAGATTTTCATCACAGGGGAATTAGGATGTTa                                                        <  1:1916677/150‑1 (MQ=255)
                                                                                            gCCGGTTCAGGAGTAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGATTTATGATGTTaaa                                                      <  1:1511644/150‑1 (MQ=255)
                                                                                            gCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCCGGCCCCACAAATCCTGCAGAGGATGAGGGATGCTGCAGTGGGCTATGGAAGGGGGATCAGATTTTCATCACAGGGGAATTATGATGTTaaa                                                      >  2:1517758/1‑150 (MQ=255)
                                                                                                  tCATGAGGAAATCCTGATCCGTCTTTTCCTTAAGTTATCCGCCCCCGTCCTCCTCGCCCAAATATCCTTCAGATGATGAGTGATCCTTCATTATGCTATGGCAAGGTGATCAGATTTTCATCACTGGGGAATTATGATGTTAAATGCAtt                                                <  1:1481684/150‑2 (MQ=255)
                                                                                                  tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATg                                                <  1:1481680/150‑1 (MQ=255)
                                                                                                   cAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCCAATAAACTTAAGATGATGAGTGATACTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATTATGTTAAATTCAttt                                               >  2:1958866/1‑148 (MQ=255)
                                                                                                          aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGc                                        <  1:1564479/150‑1 (MQ=255)
                                                                                                                 gATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGcc                                 <  2:1254870/150‑1 (MQ=255)
                                                                                                                  aTCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGccc                                <  2:1370905/150‑1 (MQ=255)
                                                                                                                  aTCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGccc                                <  2:508488/150‑1 (MQ=255)
                                                                                                                  aTCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGccc                                <  2:247501/150‑1 (MQ=255)
                                                                                                                   tCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGcccc                               <  1:751507/150‑1 (MQ=255)
                                                                                                                   tCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGccccc                              >  2:187196/1‑150 (MQ=255)
                                                                                                                    ccGGCTATTCCGAAAGATATCCGCCCCCGCCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCGGCAGGTGccatg                              <  2:229851/150‑4 (MQ=255)
                                                                                                                      ggCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATACTGCATTATGCTATGGGAAGGTGATGAGATTTTCATAACAGTGGAATTATGATTTTTAATTAATTGAACCTGCCGGTGCCCCCaa                            >  2:489132/1‑149 (MQ=255)
                                                                                                                         tATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTg                         <  1:221803/150‑1 (MQ=255)
                                                                                                                         tATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTg                         <  2:47587/150‑1 (MQ=255)
                                                                                                                           ttCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGtt                       <  2:1619291/150‑1 (MQ=255)
                                                                                                                           ttCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGtt                       <  1:1539308/150‑1 (MQ=255)
                                                                                                                            tCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                      <  2:805174/150‑1 (MQ=255)
                                                                                                                               gAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGAGCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAc                   <  1:104219/150‑1 (MQ=255)
                                                                                                                                   gTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaag               <  1:1461766/150‑1 (MQ=255)
                                                                                                                                    ttATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagc              <  1:86624/150‑1 (MQ=255)
                                                                                                                                    ttATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagc              <  2:923858/150‑1 (MQ=255)
                                                                                                                                         cGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa         <  1:1834604/150‑1 (MQ=255)
                                                                                                                                          gCCCCCGTCCTCCTGCCCCAAATATCCTTCAGATGATGAGTGATCATGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATTGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAt        <  1:345997/150‑1 (MQ=255)
                                                                                                                                          gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATGTGTTAAACAAAGCAAAGAt        >  2:1482768/1‑150 (MQ=255)
                                                                                                                                           cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc       <  2:804749/150‑1 (MQ=255)
                                                                                                                                               cGTCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTATGCTATGGAAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt   <  2:1350172/150‑1 (MQ=255)
                                                                                                                                                gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGTAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg  <  1:1769359/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTG  >  NC_000913/422284‑422577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: