Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	756,968	C→G	H354Q (CAC→CAG)	sdhA →	succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	756,968	0	C	G	100.0%	303.3 / NA	88	H354Q (CAC→CAG)	sdhA	succinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (42/46);  total (42/46)

CCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGC  >  NC_000913/756822‑757112                                                                                                                                                   |                                                                                                                                                 ccGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTAc                                                                                                                                               <  2:900249/150‑1 (MQ=255)   gTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACat                                                                                                                                             <  1:1397224/150‑1 (MQ=255)        ggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATgg                                                                                                                                        <  2:1685167/150‑1 (MQ=255)          ccACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGc                                                                                                                                      >  1:550534/1‑150 (MQ=255)           cacaCGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCg                                                                                                                                     >  2:1164835/1‑150 (MQ=255)           cacaCGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCg                                                                                                                                     >  2:1534850/1‑150 (MQ=255)            acacGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCTGTGAAAGACCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCgg                                                                                                                                    >  1:351541/1‑150 (MQ=255)            acacGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCgg                                                                                                                                    <  2:1399925/150‑1 (MQ=255)             cacGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGt                                                                                                                                   <  1:551998/150‑1 (MQ=255)                   aaactgaaactCGATCACCTGGGTAAAGAAGTGCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCg                                                                                                                             <  1:1455322/150‑1 (MQ=255)                       tgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCa                                                                                                                         >  1:1562107/1‑150 (MQ=255)                       tgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCa                                                                                                                         >  2:1542493/1‑150 (MQ=255)                       tgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCa                                                                                                                         <  2:1077148/150‑1 (MQ=255)                        gaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTTAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGTCGGAATACCGCCCat                                                                                                                        <  1:1582890/150‑2 (MQ=255)                          aactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTAGCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAg                                                                                                                      <  2:1931430/150‑1 (MQ=255)                          aactCGATCACCTGGGGAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAg                                                                                                                      <  2:175850/150‑1 (MQ=255)                           actCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGt                                                                                                                     <  2:1430574/150‑1 (MQ=255)                           actCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGt                                                                                                                     >  1:558677/1‑150 (MQ=255)                           actCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGGGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGt                                                                                                                     <  2:1813083/150‑1 (MQ=255)                                aTCACCTGGGTTAAGAAGTTCTCGATTCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCg                                                                                                                <  2:546898/150‑1 (MQ=255)                                  cACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGt                                                                                                              >  2:982385/1‑150 (MQ=255)                                    ccTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCa                                                                                                            <  1:1643576/150‑1 (MQ=255)                                     cTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGAGCGGTATTCCGACCAAAGTTACCGGTCAg                                                                                                           >  1:318622/1‑150 (MQ=255)                                      tGGGTAAAGAAGTTCTCGTATCCCGTCTGCCGGGTATCCTGGAGCGTTCCCGTACCTTCGCTCACGTCGATCCGGGGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAgg                                                                                                          <  2:1217465/150‑1 (MQ=255)                                            aagaagTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactga                                                                                                    >  1:1988537/1‑150 (MQ=255)                                             agaagTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCcctgac                                                                                                   >  1:1842906/1‑150 (MQ=255)                                              gaagTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactgact                                                                                                  >  2:26027/1‑150 (MQ=255)                                                 gTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGAGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACtgtg                                                                                               <  2:318622/150‑1 (MQ=255)                                                 gTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCACACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCCACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACtgtg                                                                                               >  1:1720211/1‑150 (MQ=255)                                                    ctcGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAAt                                                                                            >  2:1997050/1‑150 (MQ=255)                                                     tcGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATg                                                                                           <  1:647523/150‑1 (MQ=255)                                                       gAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTTCCGGTCAGGCACTGACTGTGAATgag                                                                                         >  1:323423/1‑150 (MQ=255)                                                         aTCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGaa                                                                                       >  2:655534/1‑150 (MQ=255)                                                          tCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGaaa                                                                                      <  1:631499/150‑1 (MQ=255)                                                               tCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGa                                                                                 >  1:758500/1‑150 (MQ=255)                                                                 tGCCTGTTATCCTGGAGCTTTCACGTCCCTTCGCTCACGTCGATCCGTTGAAAGAGCCGATTCCGGTTTTCCCAACCTGTCAGTACATGATGGGCTGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGGGAATGAGAAAGGCGAAg                                                                               <  2:523699/150‑1 (MQ=255)                                                                 tGCCGGGTATCCTGTAGCTTTCCCGTACCTTCGCTCACGTCGATACGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGGCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAg                                                                               <  2:530991/150‑1 (MQ=255)                                                                 tGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAg                                                                               <  1:619986/150‑1 (MQ=255)                                                                 tGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGACACTGACTGTGAATGAGAAAGGCGAAg                                                                               <  2:1911497/150‑1 (MQ=255)                                                                   ccGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATTATGGGCGGTATTCCGACCAAAGTTACCGTTCAGGCACTGACTGTGAATGAGAAAGGCGAAgct                                                                             >  2:1530439/1‑148 (MQ=255)                                                                    cGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGATTGAGAAAGGCGAAGAtg                                                                            >  1:1375179/1‑150 (MQ=255)                                                                    cGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGAtg                                                                            <  2:663696/150‑1 (MQ=255)                                                                    cGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGAtg                                                                            <  2:569881/150‑1 (MQ=255)                                                                     gggTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGAtgt                                                                           >  1:676092/1‑150 (MQ=255)                                                                       gTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTgg                                                                         >  1:1298271/1‑150 (MQ=255)                                                                          tCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGgttg                                                                      <  1:641338/150‑1 (MQ=255)                                                                            cgggAGCGTTCCCGTACCTTCGCTCCCGGCGATCCGGGGAAAGAGCCGCTTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGgttgtt                                                                    <  2:1247981/148‑1 (MQ=255)                                                                            cTGGAGCTTTCCCGTACGTTCGCTCAGGTCGATCCGGGGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGgttgtt                                                                    <  2:920420/150‑1 (MQ=255)                                                                             tGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAACGGCGATGATGTGGTTGTTc                                                                   >  1:603528/1‑150 (MQ=255)                                                                             tGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTc                                                                   >  1:726714/1‑150 (MQ=255)                                                                             tGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTc                                                                   >  1:582223/1‑150 (MQ=255)                                                                              ggAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTcc                                                                  >  2:637336/1‑150 (MQ=255)                                                                              ggAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTcc                                                                  >  2:1373956/1‑150 (MQ=255)                                                                                 gCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGAATCCGGTTATCCCAACCTGTCAGTACAGGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCggg                                                               <  1:1123142/150‑1 (MQ=255)                                                                                  cTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGa                                                              <  2:666040/150‑1 (MQ=255)                                                                                          tACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGc                                                      >  1:825902/1‑150 (MQ=255)                                                                                                cGCTCACGTCGATCCGGGGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTgg                                                <  1:1146168/150‑1 (MQ=255)                                                                                                cGCTCACGTCGATCCGGCGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTgg                                                <  2:1951921/150‑1 (MQ=255)                                                                                                 gCTCACGTCGATCCGGGGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGt                                               <  1:714918/150‑1 (MQ=255)                                                                                                      cGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGTTGAAAt                                          >  1:406334/1‑150 (MQ=255)                                                                                                         cGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGc                                       >  1:1571641/1‑150 (MQ=255)                                                                                                         cGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATCAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGc                                       >  2:564389/1‑150 (MQ=255)                                                                                                          gATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGATTGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCt                                      >  2:1511561/1‑150 (MQ=255)                                                                                                          gATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCt                                      >  1:487252/1‑150 (MQ=255)                                                                                                           aTCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCtt                                     <  2:1720211/150‑1 (MQ=255)                                                                                                            tCCGGGGAAAGCGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTtg                                    <  2:351541/150‑1 (MQ=255)                                                                                                             ccGGTGAACGAGCCGAGTACGGTTATCCCAACCGCTCAGTACATGCGGGGCGGTACCCCGACCAAAGCTACCGTTCTGGGACTGACTGTGAATGAGAACGGCGAAGAGGTGGTGGTTCCGGGACTGTTTGCCGTTGGTGTACTCGcgggt                                   <  2:94326/150‑5 (MQ=255)                                                                                                             ccGGGGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTtgt                                   <  2:718123/150‑1 (MQ=255)                                                                                                              cGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCCCTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTtgtg                                  >  2:1325541/1‑150 (MQ=255)                                                                                                              cGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGATAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTtgtg                                  >  2:1272006/1‑150 (MQ=255)                                                                                                              cGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTtgtg                                  <  1:805679/150‑1 (MQ=255)                                                                                                               ggTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTgtgt                                 >  1:87595/1‑150 (MQ=255)                                                                                                                gTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTa                                <  1:768339/150‑1 (MQ=255)                                                                                                                  gAAATAGACGATTCCGGTTATCCCAACCTGTCAGTTCATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCTAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATc                              <  1:1176016/150‑1 (MQ=255)                                                                                                                    aaGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGGCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCgg                            <  1:886600/150‑1 (MQ=255)                                                                                                                      gagCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTa                          <  1:1164835/150‑1 (MQ=255)                                                                                                                       agCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTac                         >  1:599948/1‑150 (MQ=255)                                                                                                                        cccGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTaca                        <  1:512284/149‑1 (MQ=255)                                                                                                                         ccGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTacac                       <  1:174418/150‑1 (MQ=255)                                                                                                                            aTTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGgc                    <  1:566579/150‑1 (MQ=255)                                                                                                                            aTTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGgc                    <  2:758500/150‑1 (MQ=255)                                                                                                                                    tATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCg            >  2:1750996/1‑150 (MQ=255)                                                                                                                                      tCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTc          <  2:620802/150‑1 (MQ=255)                                                                                                                                      tCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGAGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTc          >  1:1358990/1‑150 (MQ=255)                                                                                                                                       cccAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCt         >  1:110580/1‑150 (MQ=255)                                                                                                                                       cccAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTCGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCt         >  2:2020886/1‑150 (MQ=255)                                                                                                                                       cccAACCTGTCAGTACATGATGGGCGGTAATCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTAGCCGTTGGTGAAATCGCTTGTGTATCGGTACACCGCGCTAACCGTCt         >  2:286954/1‑150 (MQ=255)                                                                                                                                              tGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGggcggc  >  1:1062827/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                 CCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGC  >  NC_000913/756822‑757112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑