Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,019,460 G→A V359I (GTC→ATC)  fliK → flagellar hook‑length control protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,019,4600GA98.1% 369.5 / ‑2.5 105V359I (GTC→ATC) fliKflagellar hook‑length control protein
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (59/44);  total (59/46)
Fisher's exact test for biased strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

Only 100 of 105 total aligned reads displayed.

GTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTT  >  NC_000913/2019365‑2019554
                                                                                               |                                                                                              
gTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCAtct                                                                                             <  2:864109/99‑1 (MQ=255)
gTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCAtct                                                                                             >  2:1086887/1‑99 (MQ=255)
gTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCAtct                                                                                             >  1:1213386/1‑99 (MQ=255)
 tCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCAtctc                                                                                            >  1:1509899/1‑99 (MQ=255)
   agcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCtt                                                                                          >  2:1927201/1‑99 (MQ=255)
   agcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCtt                                                                                          >  2:2776119/1‑99 (MQ=255)
     cagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTa                                                                                        >  1:2462339/1‑99 (MQ=255)
     cagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTa                                                                                        <  1:1656880/99‑1 (MQ=255)
       gcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACa                                                                                      >  2:1565581/1‑99 (MQ=255)
       gcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACa                                                                                      >  2:1566043/1‑99 (MQ=255)
         agGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAg                                                                                    >  1:1320344/1‑99 (MQ=255)
         agGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAg                                                                                    >  1:1320424/1‑99 (MQ=255)
         agGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAg                                                                                    >  1:1320562/1‑99 (MQ=255)
           gCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAggg                                                                                  >  2:673540/1‑99 (MQ=255)
            ccGCTTCGCCGCAACAGCAAAGCCAACGCACAGCAAACCAGGAACCTCTGTCGCGGGACGACGTCGACACGCTGCCGGGTCCCATCTCTTTACAAGGGc                                                                                 >  2:2576759/1‑99 (MQ=255)
            ccGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGc                                                                                 <  2:1674120/99‑1 (MQ=255)
            ccGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGc                                                                                 <  1:2455234/99‑1 (MQ=255)
            ccGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGc                                                                                 <  1:2230306/99‑1 (MQ=255)
              gCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgt                                                                               <  2:370574/99‑1 (MQ=255)
                ttCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgtgt                                                                             <  1:2746192/99‑1 (MQ=255)
                ttCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgtgt                                                                             >  2:1482065/1‑99 (MQ=255)
                ttCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgtgt                                                                             <  1:2213965/99‑1 (MQ=255)
                    cagcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACa                                                                         <  1:2751663/99‑1 (MQ=255)
                       caacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGc                                                                      >  2:1794550/1‑99 (MQ=255)
                        aacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCa                                                                     >  2:1790299/1‑99 (MQ=255)
                           agcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACa                                                                  >  2:2753182/1‑99 (MQ=255)
                            gcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAg                                                                 >  1:2394674/1‑99 (MQ=255)
                            gcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAg                                                                 >  1:2820347/1‑99 (MQ=255)
                             caaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAgc                                                                >  2:1026067/1‑99 (MQ=255)
                              aaaGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAgcg                                                               <  1:1159602/99‑1 (MQ=255)
                              aaaGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAgcg                                                               <  1:935155/99‑1 (MQ=255)
                               aaGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAgcgg                                                              >  2:1546884/1‑99 (MQ=255)
                                aGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAgcggc                                                             <  1:212751/99‑1 (MQ=255)
                                   cAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGtt                                                          <  1:2841059/99‑1 (MQ=255)
                                   cAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGtt                                                          <  2:2586660/99‑1 (MQ=255)
                                   cAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGtt                                                          >  1:2456255/1‑99 (MQ=255)
                                      cGCACAGCAAACCATGAGCCGCTGGCGGGGGACGACGACGATACGCTTCCGGTTCCCATCTCTTGACAAGGGCGTGTAACAGGCAACNGCGGCGTGGat                                                       <  2:1743725/99‑1 (MQ=255)
                                         acaGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTACCAGGCAACAGCGGCGTTGATAtt                                                    >  2:1685773/1‑99 (MQ=255)
                                         acaGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtt                                                    >  1:2734630/1‑99 (MQ=255)
                                         acaGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtt                                                    >  1:2466762/1‑99 (MQ=255)
                                         acaGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtt                                                    >  1:2466779/1‑99 (MQ=255)
                                         acaGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtt                                                    >  2:464469/1‑99 (MQ=255)
                                         acaGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtt                                                    >  2:1913854/1‑99 (MQ=255)
                                           aGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtttt                                                  >  2:112190/1‑99 (MQ=255)
                                            gCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTc                                                 <  2:1667782/99‑1 (MQ=255)
                                            gCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTc                                                 >  1:231852/1‑99 (MQ=255)
                                            gCAAACCATGAACCTCTGGCGGGCGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTc                                                 <  2:2818884/99‑1 (MQ=255)
                                             cAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCg                                                >  1:161480/1‑99 (MQ=255)
                                             cAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCg                                                <  1:2856676/99‑1 (MQ=255)
                                              aaaCCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                                               <  2:661071/99‑1 (MQ=255)
                                              aaaCCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                                               <  2:661082/99‑1 (MQ=255)
                                              aaaCCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                                               >  2:1234877/1‑99 (MQ=255)
                                              aaaCCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                                               >  1:2122299/1‑99 (MQ=255)
                                              aaaCCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                                               >  1:790292/1‑99 (MQ=255)
                                                  cATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTaa                                           >  1:2165464/1‑99 (MQ=255)
                                                  cATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTaa                                           <  1:1010826/99‑1 (MQ=255)
                                                  cATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTaa                                           <  1:1516151/99‑1 (MQ=255)
                                                   aTGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAAc                                          >  1:1199182/1‑99 (MQ=255)
                                                     gAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGt                                        >  2:2124893/1‑99 (MQ=255)
                                                     gAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGt                                        >  2:2124757/1‑99 (MQ=255)
                                                      aaCCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTc                                       >  2:1880907/1‑99 (MQ=255)
                                                        ccTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCag                                     <  2:2576655/99‑1 (MQ=255)
                                                        ccTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCag                                     <  2:471927/99‑1 (MQ=255)
                                                        ccTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCag                                     <  2:293590/99‑1 (MQ=255)
                                                         ctctGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCaga                                    <  2:1270755/99‑1 (MQ=255)
                                                         ctctGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCaga                                    <  2:383614/99‑1 (MQ=255)
                                                          tctGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagag                                   <  1:597536/99‑1 (MQ=255)
                                                          tctGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagag                                   <  1:598152/99‑1 (MQ=255)
                                                          tctGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagag                                   <  1:2470699/99‑1 (MQ=255)
                                                             ggCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTa                                >  1:1074087/1‑99 (MQ=255)
                                                                gggggAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCa                             <  1:1238222/99‑1 (MQ=255)
                                                                gggggAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCa                             <  2:1439651/99‑1 (MQ=255)
                                                                   ggAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCg                          <  1:948363/99‑1 (MQ=255)
                                                                    gAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGt                         >  2:208143/1‑99 (MQ=255)
                                                                     aaGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTa                        <  1:2468574/99‑1 (MQ=255)
                                                                        acgacgATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATc                     >  2:2764419/1‑99 (MQ=255)
                                                                        acgacgATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATc                     >  2:1313964/1‑99 (MQ=255)
                                                                        acgacgATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATc                     <  2:1067532/99‑1 (MQ=255)
                                                                        acgacgATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATc                     >  2:2049683/1‑99 (MQ=255)
                                                                        acgacgATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATc                     >  2:2049217/1‑99 (MQ=255)
                                                                         cgacgaTACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATcc                    >  2:67767/1‑99 (MQ=255)
                                                                          gacgaTACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCcg                   <  1:2152253/99‑1 (MQ=255)
                                                                          gacgaTACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCcg                   >  2:2786494/1‑99 (MQ=255)
                                                                           acgaTACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCcgc                  <  1:1299931/99‑1 (MQ=255)
                                                                             gaTACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGt                <  2:2129573/99‑1 (MQ=255)
                                                                             gaTACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGt                <  1:1461543/99‑1 (MQ=255)
                                                                              aTACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTc               <  2:2443621/99‑1 (MQ=255)
                                                                               tACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCt              >  2:1474066/1‑99 (MQ=255)
                                                                                 cGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCttt            <  2:254716/99‑1 (MQ=255)
                                                                                   cTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTc          <  2:1064930/99‑1 (MQ=255)
                                                                                   cTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTc          >  1:2271379/1‑99 (MQ=255)
                                                                                    ttCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTcc         >  2:660791/1‑99 (MQ=255)
                                                                                    ttCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTcc         >  2:660858/1‑99 (MQ=255)
                                                                                    ttCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTcc         >  2:661112/1‑99 (MQ=255)
                                                                                     tCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTccc        >  1:2456398/1‑99 (MQ=255)
                                                                                      ccGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTcccc       >  2:2063915/1‑99 (MQ=255)
                                                                                         gTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCt    >  1:78990/1‑99 (MQ=255)
                                                                                         gTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCt    >  1:78982/1‑99 (MQ=255)
                                                                                          ttCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCtt   >  2:1252585/1‑99 (MQ=255)
                                                                                           tCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCttt  <  2:498937/99‑1 (MQ=255)
                                                                                               |                                                                                              
GTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTT  >  NC_000913/2019365‑2019554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑