Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 454,873 C→G 5.0% intergenic (+84/‑260) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913454,8730CG 5.0% 2738.1 / 14.4 830intergenic (+84/‑260)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base C (325/437);  new base G (49/2);  total (390/440)
Fisher's exact test for biased strand distribution p-value = 2.11e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 850 total aligned reads displayed.

TGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCT  >  NC_000913/454724‑455022
                                                                                                                                                     |                                                                                                                                                      
 gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCt                                                                                                                                                       <  2:8176303/150‑1 (MQ=255)
 gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCt                                                                                                                                                       <  2:1859579/150‑1 (MQ=255)
 gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCt                                                                                                                                                       >  2:5376880/1‑150 (MQ=255)
  ggATGGGTTGCTGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGTAGCTGTAAGCATCGCTTAAAAACGCATTTGCAACTGTGGGCGCTTTTCCATTATGTTGCTAAAGTTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTg                                                                                                                                                      <  1:3307786/150‑1 (MQ=255)
    aaGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATGTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGa                                                                                                                                                    >  2:3733286/1‑150 (MQ=255)
     aGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAt                                                                                                                                                   <  2:5844627/150‑1 (MQ=255)
        gTTGCTGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAtttt                                                                                                                                                <  1:11006831/150‑1 (MQ=255)
         ttGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTg                                                                                                                                               <  1:10800243/150‑1 (MQ=255)
         ttGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTg                                                                                                                                               <  2:13117951/150‑1 (MQ=255)
         ttGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGATTTTg                                                                                                                                               >  2:9761491/1‑150 (MQ=255)
              ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCtt                                                                                                                                          >  2:4849244/1‑150 (MQ=255)
              ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGATTTTGCGCtt                                                                                                                                          >  2:6826255/1‑150 (MQ=255)
                acacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCtttt                                                                                                                                        >  2:5630742/1‑150 (MQ=255)
                acacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCtttt                                                                                                                                        <  1:2246868/150‑1 (MQ=255)
                 cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCttttt                                                                                                                                       <  2:11533818/150‑1 (MQ=255)
                   ccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGGTGGGTGTTGTCTGGATTTTGCGCTTTTTGc                                                                                                                                     >  1:6318134/1‑150 (MQ=255)
                    cGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGcc                                                                                                                                    <  1:477048/150‑1 (MQ=255)
                    cGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGcc                                                                                                                                    <  1:7456548/150‑1 (MQ=255)
                      tCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCa                                                                                                                                  <  1:277973/150‑1 (MQ=255)
                       cTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAg                                                                                                                                 <  2:2893673/150‑1 (MQ=255)
                        tttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTTTCGGCGCTTTTCCATTATGTTGCTAATGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGc                                                                                                                                <  1:7300464/150‑1 (MQ=255)
                           gCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAtt                                                                                                                             <  1:9417374/150‑1 (MQ=255)
                           gCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAtt                                                                                                                             <  2:9453993/150‑1 (MQ=255)
                            ccTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAATCGGCCTGCGGGCCGTTTTGTTTTTTGTGGATTTTGGGGTTTTTGCCCAGCAttg                                                                                                                            >  2:12181495/1‑149 (MQ=255)
                            ccTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTTTCTTGCTTTTGCGCTTTTTGCCCAGCAtta                                                                                                                            >  1:5177681/1‑149 (MQ=255)
                            ccTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTGATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTATTGGCTTTTTGCCCAgaaatc                                                                                                                            >  1:15057959/1‑145 (MQ=255)
                             ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCa                                                                                                                           <  1:12999302/150‑1 (MQ=255)
                              tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAg                                                                                                                          <  2:15379986/150‑1 (MQ=255)
                               ctcctcCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGa                                                                                                                         <  2:345592/150‑1 (MQ=255)
                               ctcctcCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGa                                                                                                                         <  2:7110022/150‑1 (MQ=255)
                                tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGAc                                                                                                                        <  1:3188062/150‑1 (MQ=255)
                                  ctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGa                                                                                                                      >  1:8767287/1‑150 (MQ=255)
                                   tccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGaa                                                                                                                     >  1:13350502/1‑150 (MQ=255)
                                   tccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTCTTGCTTTTTCGCTTTTTGCCCAGCAATAAGACGaa                                                                                                                     >  1:76291/1‑150 (MQ=255)
                                     ccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTTCGCTTTTTTCCCAGCAATCAGACGaaaa                                                                                                                   >  1:12343134/1‑150 (MQ=255)
                                            tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCg                                                                                                            >  1:9652054/1‑150 (MQ=255)
                                            tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCg                                                                                                            >  1:9577319/1‑150 (MQ=255)
                                              gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCggg                                                                                                          >  2:12332352/1‑150 (MQ=255)
                                               aGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCt                                                                                                                                                       >  2:14408574/1‑104 (MQ=255)
                                                 cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAAt                                                                                                       <  1:4381600/150‑1 (MQ=255)
                                                    gAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCTGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATtgt                                                                                                    <  1:14564629/150‑1 (MQ=255)
                                                    gAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATtgt                                                                                                    <  1:10016309/150‑1 (MQ=255)
                                                    gAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATtgt                                                                                                    <  2:10946683/150‑1 (MQ=255)
                                                        cATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaa                                                                                               <  2:6525353/150‑1 (MQ=255)
                                                            gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGATTTTTCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAAt                                                                                           >  2:10573264/1‑150 (MQ=255)
                                                             cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAATGATTTTATGAAAATCGGCCTGCGGGCCGTTTTGTTTTGTGTGGATTTTGGGCTTTTTGGCCAGCATTCAGACGAAAATTTGCCGGGAATTGTGTAAAAATa                                                                                          >  2:2426255/1‑150 (MQ=255)
                                                             cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATa                                                                                          <  2:7832044/150‑1 (MQ=255)
                                                                  aaaCGCATTTGAAACTTTCGGCGCTTTTCCTTTATGTTGCTAATTTTTTTTTGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGa                                                                                     <  1:5810921/150‑1 (MQ=255)
                                                                    aCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTTGATTTTGCGCTTTTTGCCCAGCATTCCGACGAAAATTTCCCGGGGATTGTGAAAAAATAAGCGACa                                                                                   >  1:13968992/1‑150 (MQ=255)
                                                                      gCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgc                                                                                 >  2:12744690/1‑150 (MQ=255)
                                                                        aTTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAATATTTTATTAAAAACGGCCGGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATTGCCCGGGATTTGTGAAAAAATACGCGACAgcgc                                                                               <  1:841828/150‑1 (MQ=255)
                                                                         tttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgcgcg                                                                              <  2:1016118/150‑1 (MQ=255)
                                                                            gCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTGTGGATTTTTGGCTTTTTTGCCCGCCATCAGACCAAAATTGCCCGGGGAATGTGAAAAAAAAAGCGACAGCGCGCac                                                                           >  1:13492169/1‑149 (MQ=255)
                                                                              aaCTGTCGGCGCTTTTCCAGGATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCACTa                                                                         >  2:8988574/1‑150 (MQ=255)
                                                                               ccTGTGGGCGCTTTTCAATTATTTTGCTAATTATTTTATTAAAATCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCTTTCAGACTAAAATTGCCGGGGAATTGTGAAAAAATACGCGACAGCGCGCAATaa                                                                        <  1:12041047/149‑1 (MQ=255)
                                                                                 tGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAcc                                                                      >  2:11247044/1‑150 (MQ=255)
                                                                                  gTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTTTGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAAATACGCGACAGCGCGCAATAAcc                                                                      <  2:6188815/150‑1 (MQ=255)
                                                                                   tCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGt                                                                    >  2:8400536/1‑150 (MQ=255)
                                                                                    cGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGtt                                                                   <  2:7574308/150‑1 (MQ=255)
                                                                                    cGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGtt                                                                   <  2:5182077/150‑1 (MQ=255)
                                                                                    cGGCGATTTTACTGTATGTTTCTAAAGATTTTATGAAAAACGGCGTGCGGGCCTTTTTTTTTTGTCTGTTTTTTGCGATTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGtt                                                                   <  1:9840126/150‑1 (MQ=255)
                                                                                         cTTTTCCAGTATGTTGCTAAAGATTTTATGAAACACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                                                              <  2:14105104/150‑1 (MQ=255)
                                                                                         cTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGGGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                                                              <  1:14262031/150‑1 (MQ=255)
                                                                                            ttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTc                                                           <  2:13526715/150‑1 (MQ=255)
                                                                                            ttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGACCAGCATTAAGACGAAAATTGCCCGGGAATTGTGAAAAAATAAGCGACAGCGCGCAAAAAACGTTCTCGACTc                                                           >  2:13265088/1‑150 (MQ=255)
                                                                                                  tATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaaa                                                     <  1:8906099/150‑1 (MQ=255)
                                                                                                     gTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTATGGATTTTGCGCTTTTTTCCCAGCAATCAGACGAAAAATGCCCGGGAAATGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTg                                                  >  2:11990063/1‑150 (MQ=255)
                                                                                                       tGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCGATAACCGTTCTCGACTCATAAAAGTGAt                                                <  1:8059939/150‑1 (MQ=255)
                                                                                                       tGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt                                                <  1:3699060/150‑1 (MQ=255)
                                                                                                         cTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                              <  2:319085/150‑1 (MQ=255)
                                                                                                          tAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGcc                                             >  2:10440355/1‑150 (MQ=255)
                                                                                                          tAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGcc                                             >  2:4521634/1‑150 (MQ=255)
                                                                                                           aaaGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCg                                            <  1:6104330/150‑1 (MQ=255)
                                                                                                            aaGATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTTTGTGGGTTTTTCGGTTTTTTGCCAGCATTGAGTCGAAAATTGCCCGGGAATTGTGAAAAAATAAGCGACAGCGCGCAATAAACGTTCTCGACTCATAAAAGTGATTCCGc                                           >  2:6656624/1‑150 (MQ=255)
                                                                                                            aaGATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGc                                           <  1:7078090/150‑1 (MQ=255)
                                                                                                              gATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCta                                         <  2:13404747/150‑1 (MQ=255)
                                                                                                               aTTTTATGAAAAACGGCCTGCGGGCGGTTTTGTTTTGTCTGGTTTTTGCGCTTTTTGCCCAGCTTTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCtat                                        <  1:4147068/150‑1 (MQ=255)
                                                                                                               aTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGaaaa                                                                                                                   <  1:15252178/76‑1 (MQ=255)
                                                                                                               aTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGaaaa                                                                                                                   >  2:15252152/1‑76 (MQ=255)
                                                                                                                 tttATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATaa                                      <  2:1573653/150‑1 (MQ=255)
                                                                                                                  ttATGAACAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAAt                                     <  2:14918785/150‑1 (MQ=255)
                                                                                                                    aTGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGc                                   <  1:13190335/150‑1 (MQ=255)
                                                                                                                      gAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAACGTTCTCGACACATAAAAGTGATGCCGCTATAATTCcg                                 >  2:13713149/1‑150 (MQ=255)
                                                                                                                       aaaaaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgc                                <  2:4991887/150‑1 (MQ=255)
                                                                                                                        aaaaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTGGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgcg                               <  2:6318134/150‑1 (MQ=255)
                                                                                                                              gCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAt                         <  1:15499410/150‑1 (MQ=255)
                                                                                                                                cTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAttt                       <  1:1922364/150‑1 (MQ=255)
                                                                                                                                cTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAttt                       <  1:11323482/150‑1 (MQ=255)
                                                                                                                                 tGCGGGCCGTTTTGTTTTGTCTTGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTTCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAACGTTCTCGAGTCATTAAAGTGATTGCGCTATAATGCCGCGTGCTATTTg                      >  2:8749993/1‑150 (MQ=255)
                                                                                                                                  gCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGa                     <  2:795146/150‑1 (MQ=255)
                                                                                                                                      gCCGTTTTTTTTTTTCTGGATTTTTCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTTTGAAAAAATACGCGAAAGCGCGCAATAACCCTTCTAGACTCATAAAAGTGATTCAGCTATAATTCCGCGTCCTATATTAAttc                 >  2:117970/1‑148 (MQ=255)
                                                                                                                                          ttttgttttgTCTGTATTTTGTGCTTTTTGCCCAGCATTCAGACTAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc             <  1:5570108/150‑1 (MQ=255)
                                                                                                                                          ttttgttttgTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc             >  2:927264/1‑150 (MQ=255)
                                                                                                                                          ttttgttttgTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc             >  1:6547413/1‑150 (MQ=255)
                                                                                                                                            ttgttttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCgg           <  2:4241680/150‑1 (MQ=255)
                                                                                                                                            ttgttttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCgg           <  1:9083541/150‑1 (MQ=255)
                                                                                                                                            ttgttttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCgg           >  1:10247746/1‑150 (MQ=255)
                                                                                                                                               ttttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgat        >  2:2234229/1‑150 (MQ=255)
                                                                                                                                               ttttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCAAAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgat        >  1:11869272/1‑150 (MQ=255)
                                                                                                                                                   gtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGAtt    >  1:11624585/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCT  >  NC_000913/454724‑455022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: