Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 580,727 G→T 7.1% intergenic (‑282/‑107) ylcI ← / → nohD DUF3950 family protein, DLP12 prophage/DLP12 prophage; DNA packaging protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913580,7270GT 7.1% 2490.5 / 40.3 792intergenic (‑282/‑107)ylcI/nohDDUF3950 family protein, DLP12 prophage/DLP12 prophage; DNA packaging protein
Reads supporting (aligned to +/- strand):  ref base G (418/291);  new base T (5/78);  total (423/369)
Fisher's exact test for biased strand distribution p-value = 2.64e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 808 total aligned reads displayed.

CGTTGTGACATGTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGA  >  NC_000913/580578‑580876
                                                                                                                                                      |                                                                                                                                                     
     tgACATGTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGtattt                                                                                                                                                  >  2:11080757/1‑150 (MQ=255)
      gACATGTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGtattta                                                                                                                                                 <  1:10981418/150‑1 (MQ=255)
           gTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTaa                                                                                                                                            >  1:7590048/1‑150 (MQ=255)
              gatgatAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAAt                                                                                                                                         >  2:4148876/1‑150 (MQ=255)
               atgatAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATa                                                                                                                                        >  1:10288611/1‑150 (MQ=255)
                 gatAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAcc                                                                                                                                      >  2:14656625/1‑150 (MQ=255)
                  atAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAccc                                                                                                                                     <  1:5618390/150‑1 (MQ=255)
                    aaTTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAccccc                                                                                                                                   >  1:4317829/1‑150 (MQ=255)
                    aaTTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAccccc                                                                                                                                   >  2:7031805/1‑150 (MQ=255)
                    aaTTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAccccc                                                                                                                                   <  1:8102091/150‑1 (MQ=255)
                     attattATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCt                                                                                                                                  <  1:5795014/150‑1 (MQ=255)
                        attatCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTAGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGaa                                                                                                                               <  1:13522045/150‑1 (MQ=255)
                          tatCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGaaaa                                                                                                                             <  1:1523195/150‑1 (MQ=255)
                          tatCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGaaaa                                                                                                                             <  2:15249478/150‑1 (MQ=255)
                          tatCATTTTGTGGGCCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGaaaa                                                                                                                             >  1:6147260/1‑150 (MQ=255)
                            tCATTTTGTGTGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACACCCTGAaaaga                                                                                                                           >  2:1729144/1‑150 (MQ=255)
                             cATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaa                                                                                                                          <  2:9438978/150‑1 (MQ=255)
                             cATTTTGGGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaatgaa                                                                                                                          <  1:6323169/150‑1 (MQ=255)
                              aTTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaa                                                                                                                         <  2:13158718/150‑1 (MQ=255)
                              aTTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaa                                                                                                                         <  1:9709966/150‑1 (MQ=255)
                              aTTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaa                                                                                                                         <  1:4666917/150‑1 (MQ=255)
                              aTTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaa                                                                                                                         <  1:4053281/150‑1 (MQ=255)
                              aTTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaa                                                                                                                         <  1:10798925/150‑1 (MQ=255)
                              aTTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaa                                                                                                                         <  1:12374307/150‑1 (MQ=255)
                              aTTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaa                                                                                                                         <  2:9146107/150‑1 (MQ=255)
                               ttttGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaag                                                                                                                        >  2:2108318/1‑150 (MQ=255)
                               ttttGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTAATTAAAATACCCCCTGAaaagaaag                                                                                                                        >  1:2893805/1‑150 (MQ=255)
                                   gtgGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGaaa                                                                                                                    <  2:10299984/150‑1 (MQ=40)
                                      ggTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGa                                                                                                                 >  2:11027404/1‑150 (MQ=39)
                                      ggTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGa                                                                                                                 >  1:5027969/1‑150 (MQ=39)
                                        tCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTaaaa                                                                                                                                          <  2:12649499/123‑1 (MQ=40)
                                          cTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAgg                                                                                                             >  1:14453454/1‑150 (MQ=39)
                                                ggCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGa                                                                                                       >  2:777456/1‑150 (MQ=39)
                                                  cGATCCGACTGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGTTGTTTCCGTTATTCTTCGTCGTAACTTAATTTATTTATTTAAAATACCCCCTGAAAAGAAAGTAAACGACAGGTGCTGaaa                                                                                                     <  2:4707146/150‑1 (MQ=11)
                                                   gATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAg                                                                                                    >  2:8892288/1‑150 (MQ=38)
                                                    aTCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGACACGACAGGTGCTGAAAGc                                                                                                   >  2:2937827/1‑150 (MQ=38)
                                                      ccGACAGTTTACGGGGCGGCGACCTCGCGGGTTTTCTCTATTTATGAAAATTTTCCGGTTTAAGTTGTTTCCGTTCTTCTTCGTCGTAAATTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGa                                                                                                 <  2:10065166/150‑1 (MQ=255)
                                                        gACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaa                                                                                                                         >  1:11189189/1‑124 (MQ=38)
                                                           aGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTCTTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCttt                                                                                            >  2:11807430/1‑150 (MQ=255)
                                                             gTTCCGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGTTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCttttt                                                                                          <  2:5248883/150‑1 (MQ=255)
                                                             gTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCttttt                                                                                          <  2:5006279/150‑1 (MQ=40)
                                                              ttACGGGGCGGCGACCTCGCGGGTTTTCGCTCTTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTg                                                                                         >  2:200019/1‑150 (MQ=255)
                                                                     gcggcgACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTg                                                                                  >  1:13817870/1‑150 (MQ=255)
                                                                     gcggcgACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTCCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTg                                                                                  <  2:6559564/150‑1 (MQ=255)
                                                                       ggcgACCTCGCGGTTTTTCGCTATTTATGAAATTTTACCGGTTTAAGTTGTTTCCGTTCTTCTTCGTCTTAAATTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGACACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTc                                                                                <  2:7988015/150‑1 (MQ=14)
                                                                       ggcgACCTCGCGGTTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCTTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTc                                                                                <  2:6063336/150‑1 (MQ=255)
                                                                         cgACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGt                                                                              >  2:8194241/1‑150 (MQ=255)
                                                                          gACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACGGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGtt                                                                             >  2:3173155/1‑150 (MQ=255)
                                                                          gACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGtt                                                                             >  2:3662331/1‑150 (MQ=255)
                                                                            ccTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAATGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTc                                                                           <  1:14042627/150‑1 (MQ=255)
                                                                             cTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAATAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTAGtttac                                                                          >  1:12654353/1‑148 (MQ=255)
                                                                              tCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCt                                                                         >  1:3152135/1‑150 (MQ=255)
                                                                              tCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCt                                                                         <  2:14940355/150‑1 (MQ=255)
                                                                              tCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGCTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCt                                                                         >  2:12363372/1‑150 (MQ=255)
                                                                                  ggtttttCTCTATTTATCAAAATTGTCCGTTTAATGTTTTTCCCTTTCTTAGGGGTCTTAACTTATTTTTTTTATTTAAAAAACCCCCTTAAAACAACGGACACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTtct                                                                     <  1:2427478/147‑1 (MQ=2)
                                                                                  gggTTTTCGCTATTTATGAAAATTTGCCGGTTTAAGGGGTTACCTTACTCCTCCGTCGTAAATTAATGTATTTATTTAAAATACCCCCTGAAAAGAACGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTtct                                                                     <  2:13632/150‑1 (MQ=14)
                                                                                  gggTTGTCTCTTTTTATGAAAATTTGCCGGTTTATGGTGTTTCCGTTCTTCTTCGTCGTAACTTAAGGTATTTATTTAAAATACCCCCTGAAAAGAACGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTtct                                                                     <  2:12085173/150‑1 (MQ=14)
                                                                                   ggTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACATAATGTATTTATTAAAAATACCCCCTGAAAACAAAGGAAACGACAGGAGCTGAAAGCGACCTTTATGGCCTCTGTCGTTACCATtcac                                                                    >  1:7957423/1‑148 (MQ=14)
                                                                                       ttctatATTTATGAAAATTTTCCTTTTTAAGGTGTTACCTTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAATAATGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGtt                                                                <  2:13364216/145‑1 (MQ=14)
                                                                                       ttCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGtt                                                                >  2:9895502/1‑150 (MQ=255)
                                                                                         cGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACACCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTATCTGtttt                                                              >  1:1475524/1‑150 (MQ=255)
                                                                                            tATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaa                                                                                                                         >  2:6762746/1‑88 (MQ=255)
                                                                                              tttATTAAAATTTTCCGGTTTAAGGTGTTTCCGTTGTTCGTCGTGGTAACTTAATTTTTTTATTTAAAATACCCCCTCAAAACAACGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTcc                                                         <  2:5713580/150‑1 (MQ=14)
                                                                                              tttATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTcc                                                         >  2:5651719/1‑150 (MQ=255)
                                                                                                ttttaaatTTTTCCGTTTTATGGTGTTTCCTTTCTTTTTCGTCTTAAATTAATTTATTTATTTAAAAAACCCCCTCAAAATAAAGCAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGt                                                       <  1:10019773/146‑1 (MQ=14)
                                                                                                tATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGt                                                       >  2:3947924/1‑150 (MQ=255)
                                                                                                tATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGt                                                       <  2:3408274/150‑1 (MQ=255)
                                                                                                  tGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTgg                                                     <  2:1144062/150‑1 (MQ=255)
                                                                                                   gAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGa                                                    >  1:1063458/1‑150 (MQ=255)
                                                                                                    aaaaTTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGaa                                                   >  1:7260978/1‑150 (MQ=255)
                                                                                                    aaaaTTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAAATTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGaa                                                   <  2:5908509/150‑1 (MQ=255)
                                                                                                     aaaTTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGTCCTCTGTCGTTTCCTTTCTCTGTTTTTGTAAGTGGAAt                                                  >  1:3783138/1‑150 (MQ=255)
                                                                                                         tttCCGGTTTATGGTGTTTCCGTTCTTCTTCGTCTTAACTTAATGTATTTATTTAAAATACCCCATGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGc                                              <  2:14141229/150‑1 (MQ=255)
                                                                                                              ggTTTATGGTTTTTCCTTTCTTCTCCTTCTTAAATTAATTTATTTATTAAAAATACCCCCTCAAAATAAAGTAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATgg                                         <  1:12945892/150‑1 (MQ=14)
                                                                                                                tttATGGTTTTCCCTTTCTTCTTCGTCTTAACTTATTGTATTTATTTAAAATACCCCCTTAAAACAAAGCACACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGaa                                       <  1:2236663/150‑1 (MQ=14)
                                                                                                                tttATGGTGTTTCGGTTCTTCTTGGTCGTAACTTAATTTATTTATTTAAAACACCCCCTTAAAATAATGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGaa                                       <  1:6989508/150‑1 (MQ=17)
                                                                                                                tttATGGTGTTTCCTTTCTCCTTCGTCGTAACTTAATTTATTTATTTAAAATACCCCCTCAAAATAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGaa                                       <  1:8500397/150‑1 (MQ=17)
                                                                                                                tttAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGaa                                       >  1:15483432/1‑150 (MQ=255)
                                                                                                                tttAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGaa                                       >  1:5197515/1‑150 (MQ=255)
                                                                                                                 ttAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAg                                      <  2:15475209/150‑1 (MQ=255)
                                                                                                                    tggTTTTTCTTTTGTTCTTCGTCTAAACTTAATTTATTTATTTAAAAAACCCCATGAAAATAACGCACACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTca                                   <  1:6902640/149‑1 (MQ=14)
                                                                                                                     ggTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTcaa                                  >  2:2421948/1‑150 (MQ=255)
                                                                                                                      gtgtTCCGGTTCTTCTTCGTCGTAACTTATTTTATTTATTTAAAATACCCCCTCAAAATAAAGCAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTcaac                                 <  1:1700771/150‑1 (MQ=14)
                                                                                                                         tttCCGTTCTTCTTCGTCTTAACTTAATTTATTTATTTAAAATACCCCCTGAAAAGAAAGTAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACaaa                              <  2:11240291/150‑1 (MQ=255)
                                                                                                                          ttCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACaaaa                             >  2:14539783/1‑150 (MQ=255)
                                                                                                                           ttacGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGTAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACaaaa                             <  2:13910566/147‑1 (MQ=255)
                                                                                                                           tCCGTTCTTCTTCTACTTAAATTAATGTATTTATTAAAAAAACCCCCTGAAAACAAAGCAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACaaaaa                            <  1:2537473/150‑1 (MQ=14)
                                                                                                                           tCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGGAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACaaaaa                            >  2:7341475/1‑150 (MQ=255)
                                                                                                                               gTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGTCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAagca                         >  2:10471098/1‑150 (MQ=255)
                                                                                                                               gTTCTTCTTCGACGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAACGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAagca                         <  2:10761243/150‑1 (MQ=255)
                                                                                                                                 tcttcttcGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAagcagc                       >  1:6626606/1‑150 (MQ=255)
                                                                                                                                  cttcttcGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAGAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAgct                      >  1:9132923/1‑150 (MQ=255)
                                                                                                                                    tcttcGTCGTAACTTAATGTATTTATTTAAAATACCCCCTTAAAATAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAgctgg                    <  1:9891955/150‑1 (MQ=255)
                                                                                                                                       tcgtcgTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAgctggctg                 >  2:12855754/1‑150 (MQ=255)
                                                                                                                                          tcgtAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACa              >  1:12044705/1‑150 (MQ=255)
                                                                                                                                                cTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCg        >  1:3898044/1‑150 (MQ=255)
                                                                                                                                                   aaTTTATTTATTTAAAATACCCCCTTAAAAGAAAGTAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTg     <  1:11502325/150‑1 (MQ=255)
                                                                                                                                                      gTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGa  >  2:2602236/1‑150 (MQ=255)
                                                                                                                                                      gTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGa  <  2:8564461/150‑1 (MQ=255)
                                                                                                                                                      gTATTTATTTAAAATACCCCCTCAAAATAAAGCAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGa  <  1:3464879/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CGTTGTGACATGTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGA  >  NC_000913/580578‑580876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: