Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 624,598 A→T 12.0% intergenic (‑88/‑287) fepB ← / → entC ferrienterobactin ABC transporter periplasmic binding protein/isochorismate synthase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913624,5980AT12.0% 2324.9 / 80.4 835intergenic (‑88/‑287)fepB/entCferrienterobactin ABC transporter periplasmic binding protein/isochorismate synthase 1
Reads supporting (aligned to +/- strand):  ref base A (484/214);  new base T (2/111);  total (488/347)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 871 total aligned reads displayed.

ATTCCTGAAAGCAAAAGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAA  >  NC_000913/624449‑624747
                                                                                                                                                     |                                                                                                                                                     
  tCCTGAAAGCAAAAGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAgg                                                                                                                                                     >  1:11282294/1‑150 (MQ=255)
   ccTGCAAGCAAAAGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCTCCCCAAAGGGGCGAggg                                                                                                                                                    <  2:12592429/150‑1 (MQ=255)
     tGAAAGCAAAAGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGtt                                                                                                                                                  >  1:5687609/1‑150 (MQ=255)
     tGAAAGCAAAAGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGtt                                                                                                                                                  >  2:14726864/1‑150 (MQ=255)
     tGAAAGCAAAAGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGtt                                                                                                                                                  >  2:3919535/1‑150 (MQ=255)
     tGAAAGCAAAAGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTACCCTCTCCCCAAAGGGGCGAgggga                                                                                                                                                  <  1:4258465/150‑3 (MQ=255)
           cAAAAGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTGTGGGGAGGAGGTTAGGGTg                                                                                                                                            >  1:14818457/1‑150 (MQ=255)
           cAAAAGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGa                                                                                                                                           <  1:8246130/150‑1 (MQ=255)
            aaaaGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGa                                                                                                                                           <  1:11706730/150‑1 (MQ=255)
            aaaaGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGa                                                                                                                                           >  2:5814169/1‑150 (MQ=255)
             aaaGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAg                                                                                                                                          <  2:13786937/150‑1 (MQ=255)
             aaaGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAg                                                                                                                                          <  2:2817798/150‑1 (MQ=255)
              aaGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAgg                                                                                                                                         <  2:9042759/150‑1 (MQ=255)
               aGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAggg                                                                                                                                        <  2:2151827/150‑1 (MQ=255)
                    tGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAAca                                                                                                                                   >  1:5339669/1‑150 (MQ=255)
                       tAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAAcagcc                                                                                                                                <  2:1809813/150‑1 (MQ=255)
                        aaTAGATGGGCGTTGCGGTTGTGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATACGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAAcagcca                                                                                                                               <  2:1243146/150‑1 (MQ=255)
                         aTAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAAcagccag                                                                                                                              >  1:12502222/1‑150 (MQ=255)
                           aGATGGGGGTTGCGGTAGAGGGGGGCGAGTATCAAAAATCAGCTTCCTTTTATTAATAAGTTTAAGGGCTTAATGAAAAATTCTACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTTGGGTGAGGGGAACAGCCAGCa                                                                                                                            <  2:1529040/150‑1 (MQ=37)
                              tgggCGTTGGGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCGGTTATTAATAAGGTTACGGGCGTATTGACAAAGTCGCCACCGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGCGGGTTAGGGTGAGGGGAACAGCCAGCACTg                                                                                                                         <  2:15053485/149‑1 (MQ=38)
                                 gggtggCGGTAGAGCGGGGCGCGTCTCACACATCCGCTTCCTGTTATTATTAAGGTTAAGGGCGTAATGACAAATTCGACAATGCGCACATTCCGTCCCCTCGCCCCTTTGGGGTGTGGGTTTGGGTGAGGGGAACAGCCAGCACTGGTg                                                                                                                      <  2:810710/145‑1 (MQ=38)
                                     tGCGGTTGTGGGGGGCTAGTCTCACAAATCTGGTTCCTTTTATTAATACGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCGCGCCCCTTGGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGaa                                                                                                                  <  2:10763131/150‑1 (MQ=37)
                                     tGCGGTAGAGCGGGGCGAGTCTCACAACTCAGCTTCCTGTTATTACTAAGGTTAAGGGCGTAATGACAACTTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGaa                                                                                                                  <  2:10452598/150‑1 (MQ=255)
                                        ggTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACAt                                                                                                               >  1:8529167/1‑150 (MQ=255)
                                        ggTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACAt                                                                                                               >  2:15492395/1‑150 (MQ=255)
                                         gTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACAtt                                                                                                              >  2:4439922/1‑150 (MQ=255)
                                            gagCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCACCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAAc                                                                                                           >  1:2890242/1‑150 (MQ=255)
                                             cgCGGGGCGAGTCTCACAAATCCGCTTCCTGTTATTAATACGGTTATGGGCGTAATGACAAATTCGCCAACGCGCACAATCCGTCCCCTCGCCCGTTTGGGGTGTGGGTTCGGGTGTGGGGAACAGCCAGCACTGGTGCGAACATTAAcc                                                                                                          <  2:3266321/149‑1 (MQ=255)
                                             agCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAAcc                                                                                                          >  1:4711408/1‑150 (MQ=255)
                                               cGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCt                                                                                                        >  1:199238/1‑150 (MQ=255)
                                                ggggCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTc                                                                                                       >  2:12857890/1‑150 (MQ=255)
                                                          tcACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGccc                                                                                             >  1:1448881/1‑150 (MQ=255)
                                                            acaAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGTGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACACTCACCCCAGCCCTc                                                                                           >  2:9193743/1‑150 (MQ=255)
                                                               aaTCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCAcc                                                                                        >  1:5064564/1‑150 (MQ=255)
                                                                aTCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCAccc                                                                                       >  2:14253898/1‑150 (MQ=255)
                                                                aTCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCAccc                                                                                       >  2:11707375/1‑150 (MQ=255)
                                                                aTCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCAccc                                                                                       >  2:14381995/1‑150 (MQ=255)
                                                                 cccgCTTCCTTTTATAAACAGGTTTATGGGCGTAATGACAACTCCCAAAACGCGCACACGCCGCCCCCCCGCCGCTTGGGGTTGTGGTTTTGGGTGGGGGCAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCt                                                                                      <  2:6605133/147‑1 (MQ=255)
                                                                    gCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGa                                                                                   <  2:8866954/150‑1 (MQ=255)
                                                                     cTTCCTTTTTTTAATATGTTTAAGGGCGTATTCACAAATTCGACAAAGCGCACAACGCGTCCCCTCGCCCCTTTGGGGAGTGGGTTTGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGaa                                                                                  <  2:13291085/150‑1 (MQ=25)
                                                                     cTTCCTTTTATTTATTTGGTCTCGGGCTTATTTACAAATACGAAAACGCGCACACGCCGTCCCCCGCCCCCTTGGGGGTGGGTGTTCGGGGGCGGGCAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGaa                                                                                  <  1:13710572/150‑1 (MQ=255)
                                                                           gTTATAAATAAGGTTAAGGGCGTAAACAAAAATTCCACAAAGCGCAAAATCCGTCCCCTCGCCCCTTTGGGCAGAGGTTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGgaga                                                                            <  2:7365007/150‑1 (MQ=255)
                                                                            ttattaATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGagag                                                                           >  1:15326186/1‑150 (MQ=255)
                                                                            ttattaATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGagag                                                                           >  1:3053973/1‑150 (MQ=255)
                                                                             tattaATACGGTTATGGGCTTAATGACAAATTCCACAAAGCGAACAAGCCGTCCCCTCGCCCGTTTGGGGTGCGGTTTTGGGTGTGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCTCCCTTCCAGGGTGAgg                                                                          <  1:1761573/150‑1 (MQ=255)
                                                                               ttcatacGGTTAAGGGCGTAATGACAACTTCGACAAAGCGCACAAGCCGTCCCCTCGCCCCTTTGGGGTGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAgggg                                                                        <  2:4994890/147‑1 (MQ=25)
                                                                               ttatttaGGTTATGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCGCCTTTGGGGAGTGGGTTAGGGTGTGGGGAACAGCCAGCACTGGTGCGATGATTTACCCTCTCCCCAGCCCTCACCCTGGAAGGGAGAgggg                                                                        >  2:13565394/7‑150 (MQ=255)
                                                                                 aataaGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGc                                                                      >  1:6182433/1‑150 (MQ=255)
                                                                                  ataaGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCa                                                                     >  1:4428065/1‑150 (MQ=255)
                                                                                   taaGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAg                                                                    >  1:12096097/1‑150 (MQ=255)
                                                                                   taaGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAg                                                                    >  1:9638965/1‑150 (MQ=255)
                                                                                    aaGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGa                                                                   >  1:10228100/1‑150 (MQ=255)
                                                                                       gTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACg                                                                >  2:358168/1‑150 (MQ=255)
                                                                                       gTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACg                                                                <  1:14292537/150‑1 (MQ=255)
                                                                                       gTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACg                                                                >  1:7206/1‑150 (MQ=255)
                                                                                         tttgggCTTATTGAAAATTTCTAAAACGCGCACAATCCGCCCCCGCGCCGTTTGGGGGTGTGGTTTTGGGTGTGGGCAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGgc                                                              <  1:13342661/147‑1 (MQ=255)
                                                                                          atgggCGTAATGACAACTTCGACAACGCGCACACTCCGTCCCCTCGCCCCTTGGGGGCGCGGGTTCGGGTGTGGGGAACCGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGgcg                                                             <  2:8551169/148‑1 (MQ=255)
                                                                                          aaGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGTGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGgcg                                                             <  2:9582841/150‑1 (MQ=255)
                                                                                          aaGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGgcg                                                             <  2:1122572/150‑1 (MQ=255)
                                                                                              gCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAgg                                                         >  1:13512721/1‑150 (MQ=255)
                                                                                                 tAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACa                                                      >  1:10610161/1‑150 (MQ=255)
                                                                                                    tGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATca                                                   >  1:790200/1‑150 (MQ=255)
                                                                                                      aCAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATcaca                                                 >  2:12103767/1‑150 (MQ=255)
                                                                                                      aCAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATcaca                                                 >  2:6170888/1‑150 (MQ=255)
                                                                                                      aCAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATcaca                                                 >  2:14499236/1‑150 (MQ=255)
                                                                                                        aaaTTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACAtt                                               >  1:4165753/1‑150 (MQ=255)
                                                                                                           ttCGACAACGCGCACAAGCCGCCCCCTCGCCCCTGTGGGGTGTGGGTTCGGGTGTGGGCAACAGCCAGCACTGGTGCGAACATTAACCCCCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTgcg                                            <  1:5859523/150‑1 (MQ=255)
                                                                                                           ttCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTgcg                                            >  2:244917/1‑150 (MQ=255)
                                                                                                              gACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCtt                                         >  1:6379564/1‑150 (MQ=255)
                                                                                                              gACAAAGCGCACAAACCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCAGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCtt                                         >  2:6918734/1‑150 (MQ=255)
                                                                                                               aCAACGCGCACAATCCGTCCCCTCGCCCGTTGGGGGTGTGGGTTCGGGTGTGGGGCACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTa                                        <  1:13911192/150‑1 (MQ=255)
                                                                                                                  acgcgcACAATCCGGCCCCGCGCCCCTTGGGGGAGAGGGTTTGGGTGCGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGc                                     <  2:421597/148‑1 (MQ=255)
                                                                                                                   aGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCg                                    >  1:3736636/1‑150 (MQ=255)
                                                                                                                     cgcACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGaa                                  >  1:2299826/1‑150 (MQ=255)
                                                                                                                        acaATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATcc                               >  1:6580640/1‑150 (MQ=255)
                                                                                                                          aaTCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCGGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCAt                             >  1:15518850/1‑150 (MQ=255)
                                                                                                                             cctaacccTCTCCCCAAAGGGGCGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCaa                          >  2:4694018/6‑150 (MQ=37)
                                                                                                                              cGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCaat                         >  1:13129910/1‑150 (MQ=255)
                                                                                                                                  cccTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATg                     >  1:9265042/1‑150 (MQ=255)
                                                                                                                                    cTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGTAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCt                   >  2:523004/1‑150 (MQ=255)
                                                                                                                                    cTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCt                   >  1:14981108/1‑150 (MQ=255)
                                                                                                                                     tCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCtt                  >  2:2967014/1‑150 (MQ=255)
                                                                                                                                     tCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCtt                  >  1:11031942/1‑150 (MQ=255)
                                                                                                                                     tCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCtt                  >  1:874727/1‑150 (MQ=255)
                                                                                                                                       gCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTtct                >  2:9783450/1‑150 (MQ=255)
                                                                                                                                       gCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTtct                >  1:12734953/1‑150 (MQ=255)
                                                                                                                                        ccccTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTtctc               >  2:270772/1‑150 (MQ=255)
                                                                                                                                         cccTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAgg                                                                          >  2:10949363/1‑90 (MQ=255)
                                                                                                                                         cccTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAgg                                                                          <  1:10949605/90‑1 (MQ=255)
                                                                                                                                           cTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCAtt            >  2:7666683/1‑150 (MQ=255)
                                                                                                                                            tttGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCAttt           >  2:10157323/1‑150 (MQ=255)
                                                                                                                                              tGGGGTGTGGTTTTGGGTGCGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTc         <  2:5377316/150‑1 (MQ=255)
                                                                                                                                               ggggAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAAc                                                                                                           <  1:7754934/51‑1 (MQ=255)
                                                                                                                                                gggAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCAttt           >  2:11929843/1‑146 (MQ=255)
                                                                                                                                                gggAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCAt       <  1:14155579/150‑1 (MQ=255)
                                                                                                                                                 ggAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCAtt      >  2:7953143/1‑150 (MQ=255)
                                                                                                                                                 ggAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCGGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCAtt      >  2:14089824/1‑150 (MQ=255)
                                                                                                                                                  gagaGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTg     >  1:2663565/1‑150 (MQ=255)
                                                                                                                                                  gagaGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTg     >  1:10008720/1‑150 (MQ=255)
                                                                                                                                                     agGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTaa  <  1:8989659/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTCCTGAAAGCAAAAGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAA  >  NC_000913/624449‑624747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: