Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 708,015 T→A 7.7% intergenic (+258/‑319) glnS → / → chiP glutamyl‑tRNA synthetase/chitoporin, uptake of chitosugars

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913708,0150TA 7.7% 2871.1 / 32.6 946intergenic (+258/‑319)glnS/chiPglutamyl‑tRNA synthetase/chitoporin, uptake of chitosugars
Reads supporting (aligned to +/- strand):  ref base T (366/469);  new base A (101/3);  total (474/472)
Fisher's exact test for biased strand distribution p-value = 1.83e-29
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 970 total aligned reads displayed.

ATGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGG  >  NC_000913/707866‑708164
                                                                                                                                                     |                                                                                                                                                      
aTGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCTTTCACGCCGCATCCGGCAATAAACAGCAAACAATCAAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAAt                                                                                                                                                        <  1:4299642/150‑1 (MQ=255)
    tctcTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTAt                                                                                                                                                    <  1:8640540/150‑1 (MQ=255)
     ctctGCAATATTTTGAGTTTGCGTGCTTTTGTAGGCCGGATATGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATc                                                                                                                                                   <  1:9177349/150‑1 (MQ=255)
     ctctGAAATATATTGTGTTGGCGTGCTTTTGTAGGCCGGATAAGGCGTCCACGCCGCATCCGGCAAGAAACAGAAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATc                                                                                                                                                   <  1:7039148/150‑1 (MQ=38)
      tctGCATTATTTTGAGTTTGCGTGCTTGTGTAGGCCGGATACGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAACCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATGTATcc                                                                                                                                                  <  2:1804483/150‑1 (MQ=38)
      tctGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGTGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATcc                                                                                                                                                  <  1:3123775/150‑1 (MQ=255)
       ctGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTCTTAGCGAATTAATTCCGCGTCGCAATTTATCCa                                                                                                                                                 >  1:4488348/1‑150 (MQ=255)
        tGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAATCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTAGCGAATTAATTCCGCTTCGCAATTTATCCat                                                                                                                                                >  1:12768844/1‑150 (MQ=255)
        tGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTTTCTGCTTTTCGTAGCGAATTAAATCCGCTTCGCAATTTATCCat                                                                                                                                                >  1:2608473/1‑150 (MQ=255)
          cAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAAATAATTCCGCTTCGCAATTTATCCataa                                                                                                                                              >  2:4603443/1‑150 (MQ=255)
          cAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTCTTCGCGAATTAATTACGCATCGCACTTTATACataa                                                                                                                                              >  1:13765457/1‑150 (MQ=38)
               tatTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataaaataa                                                                                                                                         >  1:3356193/1‑150 (MQ=255)
                atTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataaaataaa                                                                                                                                        <  2:12509550/150‑1 (MQ=255)
                atTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTGTTCGCGAATTAATTACGCGTCGCAAATTTTCCaaaaaataaa                                                                                                                                        >  1:13535705/1‑150 (MQ=38)
                 ttGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAaaataaat                                                                                                                                       >  2:12882063/1‑150 (MQ=255)
                 ttGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAaaataaat                                                                                                                                       >  2:4445540/1‑150 (MQ=255)
                 ttGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCATCGCAATTTATCCATAaaataaat                                                                                                                                       >  1:6308402/1‑150 (MQ=255)
                  tGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAAtt                                                                                                                                      <  2:6040954/150‑1 (MQ=255)
                  tGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAAtt                                                                                                                                      >  1:9922803/1‑150 (MQ=255)
                  tGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAAtt                                                                                                                                      >  2:14123562/1‑150 (MQ=255)
                  tGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAAtt                                                                                                                                      >  2:15526759/1‑150 (MQ=255)
                   gAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAAttt                                                                                                                                     <  1:15504599/150‑1 (MQ=255)
                   gAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAAttt                                                                                                                                     >  1:10195668/1‑150 (MQ=255)
                   gAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTCTTCGCGAAATAATTACGCTTCGCAAATTATCCATAAAATAAAttt                                                                                                                                     >  1:220097/1‑150 (MQ=255)
                     gTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTaa                                                                                                                                   <  2:4882002/150‑1 (MQ=255)
                     gTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAAATACGCGTCGCAATTTATCCATAAAAAAAAATTaa                                                                                                                                   >  1:10012788/1‑150 (MQ=38)
                       ccgatctcTTTTGTCGGCCGGATAAGGCGTACCCGCCGCAACCGCCAAGAAACAGCAAACAACCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTaaaa                                                                                                                                 <  1:4846338/143‑1 (MQ=255)
                          ggTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATaa                                                                                                                              <  1:15260037/149‑1 (MQ=255)
                          cGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATaa                                                                                                                              >  2:12185322/1‑150 (MQ=255)
                           gTGCTTTTGTAGGCCGTATAAGGCGTTCACGCCGCATCCGGAAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAAc                                                                                                                             <  1:15091458/150‑1 (MQ=255)
                           gTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAAc                                                                                                                             <  1:3777816/150‑1 (MQ=255)
                            tGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACa                                                                                                                            >  2:6285437/1‑150 (MQ=255)
                            tGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTCTTCGCGAATTAATTACGCGTCGCAATTTATCCAAAAAAAAAAATTAAAATAACa                                                                                                                            >  2:10512446/1‑150 (MQ=38)
                               ttttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAATCGCCGCGTTCAGCGGCGTTTTTTGTGGTTTTTTTGGGGTATTAATTGCGGTTGGGAATTTTTACATAAAATAAATTTTATATAACaaaa                                                                                                                         >  1:1990322/1‑150 (MQ=25)
                               ttttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTTCTTTTCTTCGCGAATTAATTACGCTTCGCAATTTATACATAAAATAAAATTAAAATAaaaaaa                                                                                                                         >  2:13792602/1‑150 (MQ=39)
                               ttttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCAAAAAATAAATTTAAAATAACaaaa                                                                                                                         >  2:12358077/1‑150 (MQ=255)
                                   gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATa                                                                                                                     <  2:6362782/150‑1 (MQ=255)
                                    tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATaa                                                                                                                    <  1:1746940/150‑1 (MQ=255)
                                    tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATaa                                                                                                                    <  2:2445248/150‑1 (MQ=255)
                                    tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTTTATGCTTTTGTTCGCGAAATAATTACGCTTAGCAAATTATACAAAAAAAAAATTTAAAATAACaaaaaaaaa                                                                                                                    >  1:13249988/1‑145 (MQ=37)
                                     ttgCCGGATGCGGCGTGAACGCCTTATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCGTCGCAATTTATCCATAAAATAAAATTAAAATAACAAAACATAAt                                                                                                                   >  2:7452458/3‑150 (MQ=255)
                                     aGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTGTGCTTTTCTTGGCGAATTAATTCCGCTTCGGAATTTATCCATAAAATAAATTTAAAATAACAAAACATAAt                                                                                                                   >  1:8322090/1‑150 (MQ=255)
                                     aGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAAt                                                                                                                   <  2:13195449/150‑1 (MQ=255)
                                     aGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTAGCAATTTATCCATAAAATAAATTTAAAATAACAAAAaataat                                                                                                                   >  2:8441624/1‑150 (MQ=255)
                                      ggCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTTTCCATAAAATAAATTTAAAATAACAAAAAATAAtt                                                                                                                  >  2:7562242/1‑150 (MQ=255)
                                       gCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATta                                                                                                                 >  2:7820133/1‑150 (MQ=255)
                                         cGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTCTTCGCGAATTAATTCCGCGTCGCCAATTATACAAAAAATAAATTTAAAATAACAAAACAtaaataaa                                                                                                               >  1:5113817/1‑150 (MQ=255)
                                         cGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCAGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATtaaa                                                                                                               <  2:10783086/150‑1 (MQ=255)
                                            aTAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATtaaataa                                                                                                            <  1:739658/150‑1 (MQ=255)
                                            aTAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTCTTCGCGAATTAATTCCGCTTCGCAAGTTATCCAAAAAAAAAATTTAAAATAAAAAAACataaataaataa                                                                                                            >  2:11874274/1‑150 (MQ=255)
                                              aaGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATaaaa                                                                                                          <  1:12958973/150‑1 (MQ=255)
                                                       aCGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTGTTGCTGCGGGTCGTCGCGCAGTAATTCCGCGGCGCACGTTCTCCCGCCAATAAATGTCAAATAACAAAACAGAATTAAATAAAATGTAccccc                                                                                                 >  2:4252613/1‑148 (MQ=255)
                                                         gccgcATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATTCTTTTCTTAGCGAATTAATTACGCTTAGCAATTTATACAAAAAAAAAAATTAAAATAAAAAAAAATAATTAAATAAAATGTAACAGCtt                                                                                               >  2:14760315/1‑150 (MQ=255)
                                                         gccgcATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTTTCTGCTTTTCTTCGCGAATTAATTAAGCTTCGCAATTTATACATAAAATAAATTTAAAATAACAAAAAATAATTAAATAAAATGTAACAGcgt                                                                                               >  2:55179/1‑148 (MQ=255)
                                                          ccgcATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCttt                                                                                              <  2:11518770/150‑1 (MQ=255)
                                                          ccgcATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTTTGTGCTTTTCTTCGCGAAATTATTACGCTTCGCAATTTATACAAAAAAAAAATTTAAAAAAAAAAAAAAAAATTAAATAAAAAGTAAACGcggt                                                                                              >  1:752977/1‑147 (MQ=255)
                                                                 cGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTg                                                                                       <  1:14167617/150‑1 (MQ=255)
                                                                  ggCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGc                                                                                      >  2:8765384/1‑150 (MQ=255)
                                                                   gCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTTCTTTTCTTCGCGAATTAATACCGCTTCGCAAATTATCCAAAAAATAAATTTAAAATTACAAAAAATAAAAAAATAAAATGTAACCGCTTTAATCTTGCt                                                                                     >  2:3489539/1‑150 (MQ=255)
                                                                    cAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTg                                                                                    >  2:1036392/1‑150 (MQ=255)
                                                                             gCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAAATCCGCATCGCAATATATCCATAAAATAAATTTAAAATAACAAAAAATAAATAAAAAAAATGTAAACGCTGTCATCTTGCTGGAATTTAAc                                                                           >  2:15132115/1‑150 (MQ=255)
                                                                                      ccAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTAtt                                                                 <  2:265158/150‑1 (MQ=255)
                                                                                      ccAAAACGCCGCGTTCAGCGGCGAATTTTCTGCATTTCTTCGCGAAATAATTCCGCTTCGCAAATTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATCTAACCGCTTTCAACTTGCTGGAATTTCACGC‑TTTTAtt                                                                 <  2:3794642/150‑1 (MQ=255)
                                                                                       cAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTAttc                                                                <  2:504583/150‑1 (MQ=255)
                                                                                        aaaaCGCCGCGTTCAGCGGCGTTTTTTCTGCATTTCATCGCGAAATAAATCCGCATACCCAATTATCCAAAAAATAAAATTAAAATAAAAAAACAAAAATAAAAAAAAATAAAACCCGTTAATCATGCTGGAATATAACGCATTTTAtaa                                                                >  2:6942543/1‑148 (MQ=255)
                                                                                         aaccgccgcGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTAttctt                                                              <  1:5423810/147‑1 (MQ=255)
                                                                                         aaaCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTAttctt                                                              <  1:12289145/150‑1 (MQ=255)
                                                                                           aCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATtcttct                                                            <  2:9312317/150‑1 (MQ=255)
                                                                                           aCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATtcttct                                                            <  2:5773790/150‑1 (MQ=255)
                                                                                               cgcgTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCaa                                                        <  1:5625795/150‑1 (MQ=255)
                                                                                                gcgTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCGTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATTTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAg                                                       >  2:978227/1‑150 (MQ=255)
                                                                                                 cgTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTACTTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGc                                                      <  2:8983821/150‑1 (MQ=255)
                                                                                                  gTTCAGCGGCGTTTTTTCTGCTTTTCGTCGCGAATTAATTACCCATAGCAAATTATACATAAAATAAATTTAAAATAAAAAAAAATAATTAAATAAAATGTAACCCCGTTAATCTTTCTGGAATTTAACGC‑TTTTATTCTTCTTCAAccc                                                     >  2:6761618/1‑150 (MQ=255)
                                                                                                     cAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCttt                                                  <  2:2794777/150‑1 (MQ=255)
                                                                                                     cAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCttt                                                  <  2:1296344/150‑1 (MQ=255)
                                                                                                      aGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTc                                                 <  1:6513764/150‑1 (MQ=255)
                                                                                                       gcggcgTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCa                                                <  2:7284739/150‑1 (MQ=255)
                                                                                                          gctttttttCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATACTTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAAcc                                             <  1:11743360/147‑1 (MQ=255)
                                                                                                            gTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCATCCGc                                           >  2:10150586/1‑150 (MQ=255)
                                                                                                                    tGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTaa                                   >  2:11165336/1‑150 (MQ=255)
                                                                                                                     gCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAg                                  <  1:12071909/150‑1 (MQ=255)
                                                                                                                      cTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGc                                 <  1:6046386/150‑1 (MQ=255)
                                                                                                                         ttcttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCtt                              <  2:12742273/150‑1 (MQ=255)
                                                                                                                         ttcttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCtt                              <  1:14568015/150‑1 (MQ=255)
                                                                                                                           cttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGt                            <  2:15335075/150‑1 (MQ=255)
                                                                                                                             tcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTaa                          <  2:4016466/150‑1 (MQ=255)
                                                                                                                                cgAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACaa                       >  2:3254073/1‑150 (MQ=255)
                                                                                                                                 gAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACaaa                      <  1:886599/150‑1 (MQ=255)
                                                                                                                                 gAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACaaa                      >  2:8082388/1‑150 (MQ=255)
                                                                                                                                 gAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACaaa                      <  2:5283108/150‑1 (MQ=255)
                                                                                                                                    ttaattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAat                   <  1:10826944/150‑1 (MQ=255)
                                                                                                                                    ttaattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAat                   <  1:952649/150‑1 (MQ=255)
                                                                                                                                       attCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAatcat                >  1:8075049/1‑150 (MQ=255)
                                                                                                                                        ttCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAatcatc               <  2:207930/150‑1 (MQ=255)
                                                                                                                                        ttCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAatcatc               <  2:13535705/150‑1 (MQ=255)
                                                                                                                                          ccGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCaa             >  1:1138346/1‑150 (MQ=255)
                                                                                                                                           cGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCATCCGCAGACTTAAGCCTTGTAACAAAAATCATCaaa            >  1:14873025/1‑150 (MQ=255)
                                                                                                                                            gCTTCGCAATTTATCCATAAAATAAATTTAAAATAACACAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCaaaa           <  2:14532201/150‑1 (MQ=255)
                                                                                                                                               tCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAatat        <  1:11111446/150‑1 (MQ=255)
                                                                                                                                                cGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATAtg       >  2:3067916/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
ATGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGC‑TTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGG  >  NC_000913/707866‑708164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑