Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 708,020 C→A 6.3% intergenic (+263/‑314) glnS → / → chiP glutamyl‑tRNA synthetase/chitoporin, uptake of chitosugars

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913708,0200CA 6.3% 2956.2 / 22.0 924intergenic (+263/‑314)glnS/chiPglutamyl‑tRNA synthetase/chitoporin, uptake of chitosugars
Reads supporting (aligned to +/- strand):  ref base C (369/453);  new base A (97/2);  total (469/455)
Fisher's exact test for biased strand distribution p-value = 1.29e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 958 total aligned reads displayed.

CTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGGTTGCT  >  NC_000913/707871‑708169
                                                                                                                                                     |                                                                                                                                                     
ctctGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATc                                                                                                                                                       <  1:15023605/150‑1 (MQ=255)
 tctGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATcc                                                                                                                                                      <  2:11308226/150‑1 (MQ=255)
 tctGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATcc                                                                                                                                                      <  1:9041656/150‑1 (MQ=255)
 tctGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGAAAACAACCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATcc                                                                                                                                                      <  1:5210217/150‑1 (MQ=255)
 tcggCAATCTATTGAGTGTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCTAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTGTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATcc                                                                                                                                                      <  2:1804434/147‑1 (MQ=38)
   tGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCat                                                                                                                                                    >  2:15517100/1‑150 (MQ=255)
     cAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataa                                                                                                                                                  <  2:10413530/150‑1 (MQ=255)
     cAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataa                                                                                                                                                  <  2:6252189/150‑1 (MQ=255)
     cAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTCTTCGCGAATTAATTACGCATCGCACTTTATACataa                                                                                                                                                  >  1:13765457/1‑150 (MQ=38)
       atatatTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTATTCGCGAATTAATTACGCTTAGCAAATTATACaaaaaa                                                                                                                                                >  2:3027946/1‑150 (MQ=37)
            ttGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTGTGCTTTTGTTGGCGAATTATTTGCGCGTAGCAATATATCCataaaaaaaaa                                                                                                                                           >  1:9279467/1‑149 (MQ=37)
               aGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATAAAAAAAATAAATTTa                                                                                                                                        >  2:6931795/1‑150 (MQ=255)
               aGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGATCAGCGGCGTGTTTTGTGGGTTTCTTAGCGAAATAATTCCGCGTCGCCATTTAACAATAAAAAAAAtata                                                                                                                                        >  2:14312793/1‑150 (MQ=25)
                gTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAAATACGCGTCGCAATTTATCCATAAAAAAAAATTaa                                                                                                                                       >  1:10012788/1‑150 (MQ=38)
                 tttGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTAGCGAAATAATTCCCCTTAGCAATTTATCCATAAAATAAATTTaaa                                                                                                                                      >  2:4351674/1‑150 (MQ=255)
                 gttGCGTGCGTTTGTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTaaa                                                                                                                                      <  1:7950029/149‑1 (MQ=255)
                  ttGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTaaaa                                                                                                                                     <  1:5560270/150‑1 (MQ=255)
                     cGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATaa                                                                                                                                  <  1:6853664/150‑1 (MQ=255)
                     cGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATaa                                                                                                                                  <  2:10664014/150‑1 (MQ=255)
                     cGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCGTTTCTTCGCGAATTAATTCCGCTTCGCAAATTATCCAAAAAAAAAATTTAaaaaaa                                                                                                                                  >  2:14732886/1‑150 (MQ=39)
                      gTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAAc                                                                                                                                 <  1:11130509/150‑1 (MQ=255)
                      gTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTAGCGAATTAATTACGCTTAGCAATTTATCCATAAAAAAAATTTAaaaaaaa                                                                                                                                 >  1:13539746/1‑149 (MQ=38)
                       tGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAAATAATTACGCTTCGCAAATTATACAAAAAAAAAATTTAaaaaaaaa                                                                                                                                >  1:1830716/1‑148 (MQ=38)
                         cTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACaaa                                                                                                                              <  2:6350521/150‑1 (MQ=255)
                          ttttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTATTAGCGAATTAATTACGCTTCGCAATTTATCAATAAAATAAAATTAAAATAaaaaaa                                                                                                                             >  2:13047672/1‑150 (MQ=38)
                            ttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACa                                                                                                                           >  2:5132055/1‑150 (MQ=255)
                            ttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAAATAATTACGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACaaaaaa                                                                                                                           >  2:15283529/1‑148 (MQ=255)
                              gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATa                                                                                                                         >  2:2599375/1‑150 (MQ=255)
                              gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTTTGGGCTTTTCTTCGCGAATTAATTCCGCTTCGCAAATTATACAAAAAAAAAAATTAAAATAACaaaaaaaa                                                                                                                         >  1:3605810/1‑146 (MQ=38)
                               tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATaa                                                                                                                        <  1:4718644/150‑1 (MQ=255)
                               tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTTTATGCTTTTGTTCGCGAAATAATTACGCTTAGCAAATTATACAAAAAAAAAATTTAAAATAACaaaaaaaaa                                                                                                                        >  1:13249988/1‑145 (MQ=37)
                                 ggCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTTTGTGCTTTTATTCGCGAAATAATTCAGCGTCGCAAGTTATGCAAAAAATAAATTTAAAATAACaaaaacttaat                                                                                                                      >  1:11806699/1‑143 (MQ=38)
                                  gCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCGTCGCGAATTAATTCCGCTTCGCAAATTATCCAAAAAATAAATTTAAAATAACAAAACATAAAta                                                                                                                     >  2:7551084/1‑150 (MQ=255)
                                   ccGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTTTCTGCGTTTCTTCGCGAAATAAATCCCCGTCGCAAATTAACCCAAAAATAAATTTAAAATAACAAAACAAAAAtaa                                                                                                                    >  2:7243040/1‑150 (MQ=255)
                                    cGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATtaaa                                                                                                                   <  2:7803084/150‑1 (MQ=255)
                                       aTAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTCTTCGCGAAATAATTCCGCTTCGCAATTTATACATAAAAAAAATTTAAAATAAAAAAACAGAATtaaataa                                                                                                                >  2:4757043/1‑150 (MQ=255)
                                         aaGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCTATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATaaaa                                                                                                              <  1:8132282/150‑1 (MQ=255)
                                         aaGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATaaaa                                                                                                              <  1:12959345/150‑1 (MQ=255)
                                         aaGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATaaaa                                                                                                              <  2:11736167/150‑1 (MQ=255)
                                          aGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAATCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGTATTAAGTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAAt                                                                                                             <  2:3440225/150‑1 (MQ=255)
                                            gCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTGTGCTTTTGTTCGCGAATTAATTACGCTTCGCAATTTATACAAAAAATAAATTTAAAATAACAAAAAATAATTAAATAAAAttt                                                                                                           >  2:1512349/1‑148 (MQ=255)
                                            gCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTTTTAGCGAATTAATTACGCTTCGCAAATTATACATAAAAAAAATTTAAAATAAAAAaaaaaaaataaaaaaaattt                                                                                                           >  1:216216/1‑148 (MQ=255)
                                            gCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGt                                                                                                           >  2:14072385/1‑150 (MQ=255)
                                            gCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGt                                                                                                           >  2:10089903/1‑150 (MQ=255)
                                            gCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTACGCGTCGCAATTTATCCATAAAAAAAATTTAAAATAAAAAAACATAATTAAATAAAATGt                                                                                                           >  2:4552076/1‑150 (MQ=255)
                                            gCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAAATCCGCGTCGCAATTTATCCATAAAATAAATTTAAAAAAACAAAACATAATTAAATAAAATGt                                                                                                           >  2:5472717/1‑150 (MQ=255)
                                              gTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTaa                                                                                                         <  1:11645841/150‑1 (MQ=255)
                                               ttCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTTAATTTACAAAACATAATTTATTATAATGTAAc                                                                                                        >  2:8956325/1‑150 (MQ=255)
                                                tCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAAAAACAAAAAATAATTAAATAAAATGTAAcc                                                                                                       >  2:11101213/1‑150 (MQ=255)
                                                 cACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCg                                                                                                      <  2:8430017/150‑1 (MQ=255)
                                                  aCGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTGTTCTTTTATTCGCGAATTAATTACGCTTAGCAATTTATACAAAAAATAAAATTAAAATAACAAAAAAAAATTAAATAAAAATTAACCGc                                                                                                     >  2:5767410/1‑150 (MQ=255)
                                                  aCGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGc                                                                                                     >  2:12023141/1‑150 (MQ=255)
                                                    gccgcATCCGGCAAGAAACAGCAAAAAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCtt                                                                                                   <  1:1698254/150‑1 (MQ=255)
                                                     ccgcATCCGGCAAGAAACAGCAAACAATCCAACACGCCGCGTTCAGAGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCttt                                                                                                  <  2:12229313/150‑1 (MQ=255)
                                                      cgcATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGTTTTc                                                                                                 <  2:3196601/150‑1 (MQ=255)
                                                      cgcATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTc                                                                                                 <  2:870626/150‑1 (MQ=255)
                                                            cGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTg                                                                                           <  1:14167617/150‑1 (MQ=255)
                                                             ggCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCAAAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCATTCATCTTGc                                                                                          >  2:12661621/1‑150 (MQ=255)
                                                                       ccgatctaaTCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCa                                                                                <  1:6478834/143‑1 (MQ=255)
                                                                       aGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAAAAAATTTAAAATAACAAAACATAATTTAATAAAATGTACCCGCTTTCATCTTGCTGGAATTTCa                                                                                >  2:9736285/1‑150 (MQ=255)
                                                                         cAAACAATCCACACCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACg                                                                              <  2:10276235/150‑1 (MQ=255)
                                                                         cAAACAATCCAACCCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACg                                                                              <  2:2732354/150‑1 (MQ=255)
                                                                               aTCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTa                                                                        <  1:5880628/150‑1 (MQ=255)
                                                                                tCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCGTTTCGTCGCCCATTAATTCCGCTTCGCAAGTTATCCAAAAAAAAAATTTAAAATAACAAAACAGAATTAAAAAAAATGTAACCGCGTTCAGCTTGCGGGAATTTCACGCGGTTAt                                                                       >  2:13351791/1‑150 (MQ=255)
                                                                                   aaaaCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCGTTTAttat                                                                    >  2:13594005/1‑148 (MQ=255)
                                                                                      aCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATtcttct                                                                 <  1:4444964/150‑1 (MQ=255)
                                                                                             gTTCAGCGGCGTTTTTTCTGCTTTTCGTCGCGAATTAATTACCCATAGCAAATTATACATAAAATAAATTTAAAATAAAAAAAAATAATTAAATAAAATGTAACCCCGTTAATCTTTCTGGAATTTAACGCTTTTATTCTTCTTCAAccc                                                          >  2:6761618/1‑150 (MQ=255)
                                                                                              ttCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCt                                                         <  2:7387908/150‑1 (MQ=255)
                                                                                                 aGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTc                                                      <  2:3743754/150‑1 (MQ=255)
                                                                                                        ttttttCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCa                                               >  1:2564280/1‑150 (MQ=255)
                                                                                                        ttttttCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCa                                               >  1:3336926/1‑150 (MQ=255)
                                                                                                            ttCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACt                                           >  1:4991785/1‑150 (MQ=255)
                                                                                                             tCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCTCGCTTTTATTGTTCTGCAAGCCTTTCATCCGCAAACtt                                          >  1:10870353/1‑150 (MQ=255)
                                                                                                             tCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACtt                                          >  1:4081184/1‑150 (MQ=255)
                                                                                                               tGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTaa                                        >  1:8741425/1‑150 (MQ=255)
                                                                                                               tGCTTTTCGTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTaa                                        <  2:205430/150‑1 (MQ=255)
                                                                                                                gCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCa                                                                                                >  1:12105327/1‑93 (MQ=255)
                                                                                                                gCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCa                                                                                                <  1:8532115/93‑1 (MQ=255)
                                                                                                                gCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAg                                       >  1:1540821/1‑150 (MQ=255)
                                                                                                                 cTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGc                                      >  1:10457988/1‑150 (MQ=255)
                                                                                                                 cTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGc                                      <  1:3027666/150‑1 (MQ=255)
                                                                                                                 cTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGc                                      <  2:4224137/150‑1 (MQ=255)
                                                                                                                   tttCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAATTTTAAAATATCTAAACTTAATTTAATAAATTGTAACCGGTTTCATGTTGCTGGAATTTCAGGCTTTTATTCTTCTGCTAGCCTTTCAAGCGCTATCTTTAGcgt                                    >  2:12365106/1‑148 (MQ=255)
                                                                                                                    ttcttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCtt                                   <  2:804756/150‑1 (MQ=255)
                                                                                                                       ttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTa                                >  1:7198794/1‑150 (MQ=255)
                                                                                                                          gcgAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACa                             <  2:393453/150‑1 (MQ=255)
                                                                                                                          gcgAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACa                             >  1:12867438/1‑150 (MQ=255)
                                                                                                                          gcgAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACa                             >  2:4925291/1‑150 (MQ=255)
                                                                                                                            gAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACaaa                           >  1:7511198/1‑150 (MQ=255)
                                                                                                                             aattaattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACaaaa                          <  2:12954734/150‑1 (MQ=255)
                                                                                                                                      cGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCaaa                 >  1:14873014/1‑150 (MQ=255)
                                                                                                                                          tCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTAttc                                                                     <  2:9281243/94‑1 (MQ=255)
                                                                                                                                             cAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAGTGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAATCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATAtgtg          >  1:15202067/1‑150 (MQ=255)
                                                                                                                                               aTTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCg        >  2:600055/1‑150 (MQ=255)
                                                                                                                                                tttATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCgg       <  2:12828803/150‑1 (MQ=255)
                                                                                                                                                tttATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCgg       >  1:971173/1‑150 (MQ=255)
                                                                                                                                                 ttATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGGt      <  2:2372085/150‑1 (MQ=255)
                                                                                                                                                 ttATCCATAAAATAAAATTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTATCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGGt      <  1:12365106/150‑1 (MQ=255)
                                                                                                                                                     ccATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGGTTGCt  <  1:14597883/150‑1 (MQ=255)
                                                                                                                                                     ccATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGGTTGCt  <  1:6232009/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGGTTGCT  >  NC_000913/707871‑708169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: