Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,151,888 T→G 14.8% intergenic (+37/‑51) acpP → / → fabF acyl carrier protein (ACP)/3‑oxoacyl‑[acyl‑carrier‑protein] synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,151,8880TG14.8% 1926.6 / 117.2 738intergenic (+37/‑51)acpP/fabFacyl carrier protein (ACP)/3‑oxoacyl‑[acyl‑carrier‑protein] synthase II
Reads supporting (aligned to +/- strand):  ref base T (356/255);  new base G (4/115);  total (361/377)
Fisher's exact test for biased strand distribution p-value = 6.22e-33
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 773 total aligned reads displayed.

AGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGT  >  NC_000913/1151739‑1152037
                                                                                                                                                     |                                                                                                                                                     
aGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGtt                                                                                                                                                       <  2:5567271/150‑1 (MQ=255)
  cTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGtttt                                                                                                                                                     >  1:4950208/1‑150 (MQ=255)
  cTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGtttt                                                                                                                                                     >  2:15550627/1‑150 (MQ=255)
   tGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTa                                                                                                                                                    <  2:5814590/150‑1 (MQ=255)
         tGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCtttt                                                                                                                                              <  2:926578/150‑1 (MQ=255)
          ggCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCttttt                                                                                                                                             <  2:13232227/150‑1 (MQ=255)
            ctctGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTGGGATCTTTTTGt                                                                                                                                           <  1:1686879/150‑1 (MQ=255)
                ggAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCa                                                                                                                                       >  1:10220173/1‑150 (MQ=255)
                ggAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCa                                                                                                                                       >  1:1878635/1‑150 (MQ=255)
                  aagaagAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACt                                                                                                                                     >  1:4652741/1‑150 (MQ=255)
                  aagaagAGTTTGATACTGAGAGTCCGGACGAAGGAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTGGGATCTTTTTGTCCCACt                                                                                                                                     <  1:8974795/150‑1 (MQ=255)
                     aagaGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGATAATCACCACCGTTCAGGCTGCGATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGa                                                                                                                                  <  2:8093079/150‑1 (MQ=255)
                      agagTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGaa                                                                                                                                 <  2:6511172/150‑1 (MQ=255)
                       gagTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAAt                                                                                                                                >  2:944801/1‑150 (MQ=255)
                          tttGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCAt                                                                                                                             >  2:13887657/1‑150 (MQ=255)
                           ttGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCCGGCGGTCGTTCGACCGCCTGAGTGGGATCTTTTTGTCCCACTAGAATCAtt                                                                                                                            <  1:5567162/150‑1 (MQ=255)
                           ttGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCAtt                                                                                                                            >  1:10990195/1‑150 (MQ=255)
                              aTACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCAttttt                                                                                                                         >  2:629329/1‑150 (MQ=255)
                               tACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCAtttttt                                                                                                                        <  2:14038897/150‑1 (MQ=255)
                               tACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCAtttttt                                                                                                                        >  2:10759282/1‑150 (MQ=255)
                                aCTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTtc                                                                                                                       <  2:5260117/150‑1 (MQ=255)
                                aCTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTGGGATCTTTTTGTCCCACTAGAATCATTTTTtc                                                                                                                       <  1:15546267/150‑1 (MQ=255)
                                 cTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTtcc                                                                                                                      <  2:4890903/150‑1 (MQ=255)
                                 cTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTtcc                                                                                                                      <  2:1127806/150‑1 (MQ=255)
                                 cTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTGGGATCTTTTTGTCCCACTAGAATCATTTTTtcc                                                                                                                      <  1:3717884/150‑1 (MQ=255)
                                     gaTTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTTAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTtccctcc                                                                                                                  <  2:8343802/150‑1 (MQ=255)
                                       ttCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTGGGATCTTTTTGTCCCACTAGAATCATTTTTTccctccct                                                                                                                <  1:6714918/150‑1 (MQ=255)
                                        tCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTg                                                                                                               >  2:7196776/1‑150 (MQ=255)
                                        tCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTg                                                                                                               <  2:9784542/150‑1 (MQ=255)
                                          cGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTGGGATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTgga                                                                                                             <  1:10040151/150‑1 (MQ=255)
                                           ggACGAAGAAGCTGAGAAAATCCCCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTggag                                                                                                            <  2:12452977/150‑1 (MQ=255)
                                           ggACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTggag                                                                                                            >  1:5682326/1‑150 (MQ=255)
                                              cGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGAc                                                                                                         >  1:9615985/1‑150 (MQ=255)
                                                 agaagCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACaaa                                                                                                      <  2:7780389/150‑1 (MQ=255)
                                                 agaagCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACaaa                                                                                                      >  1:7711841/1‑150 (MQ=255)
                                                  gaagCTGAGAAACTCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAAc                                                                                                     <  2:7682441/150‑1 (MQ=255)
                                                  gaagCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTGTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAAc                                                                                                     >  1:11505799/1‑150 (MQ=255)
                                                   aagCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACg                                                                                                    >  1:4146261/1‑150 (MQ=255)
                                                      cTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACgtgt                                                                                                 >  1:12999259/1‑150 (MQ=255)
                                                      atGAGAAAATCACCAACGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGAGAACATCTCCAGGCGGTCGTTCGACCGCCACAGATATATCTTTATCTCCCACAAGAAACATATTTACCCTACCTGTAGGACaaaagaga                                                                                                 >  2:13628462/2‑145 (MQ=255)
                                                       tGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTc                                                                                                >  1:8771688/1‑150 (MQ=255)
                                                        gagaAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTAt                                                                                                                                                   <  1:14905466/98‑1 (MQ=255)
                                                         agaAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTa                                                                                              >  1:13996811/1‑150 (MQ=255)
                                                          gaAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTaa                                                                                             <  2:6944530/150‑1 (MQ=255)
                                                            aaaTCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGc                                                                                           <  2:4656080/150‑1 (MQ=255)
                                                              aTCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGcgt                                                                                         >  2:14944781/1‑150 (MQ=255)
                                                                 accaccGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGcgtcgt                                                                                      <  2:8607029/150‑1 (MQ=255)
                                                                 accaccGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGcgtcgt                                                                                      <  2:12977885/150‑1 (MQ=255)
                                                                       gTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTGTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGtt                                                                                <  1:225923/150‑1 (MQ=255)
                                                                               tGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCgg                                                                        >  1:12473682/1‑150 (MQ=255)
                                                                               ggCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCgg                                                                        >  2:6799383/2‑150 (MQ=255)
                                                                               ggCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATATTTTTGTACCACTAGAATCAATTTTTCCCACCCAGGAGGACCAACGTGTATAAGCGTCGTGTAGTTGTGACCgg                                                                        >  1:13490824/2‑150 (MQ=255)
                                                                                  cATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACt                                                                     >  2:12313109/1‑150 (MQ=255)
                                                                                   aTTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCCGGCGGTCGTTCGACCGCCTGAGTGGGATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTg                                                                    <  1:13194418/150‑1 (MQ=255)
                                                                                   aTTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAGGCGTCGTGTAGTTGTGACCGGACTg                                                                    >  2:297187/1‑150 (MQ=255)
                                                                                    ttGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTgg                                                                   >  2:7368447/1‑150 (MQ=255)
                                                                                        ttACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGTCCGGACTGGGCAt                                                               >  1:5352302/1‑150 (MQ=255)
                                                                                        ttACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAt                                                               <  2:12814025/150‑1 (MQ=255)
                                                                                          aCATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAtgt                                                             <  1:10759282/150‑1 (MQ=255)
                                                                                            aTCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCCtgttg                                                           >  1:3250990/1‑150 (MQ=255)
                                                                                            aTCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAtgttg                                                           <  2:3236573/150‑1 (MQ=255)
                                                                                            aTCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAtgttg                                                           >  1:14148774/1‑150 (MQ=255)
                                                                                             tCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTGGATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAtgttgt                                                          <  1:4368316/150‑1 (MQ=255)
                                                                                              cAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGtc                                                         >  2:259209/1‑150 (MQ=255)
                                                                                              cAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTGGGATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGtc                                                         <  1:629329/150‑1 (MQ=255)
                                                                                               aaCGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATATTTTTGTCCCACTAGAATCATTTTTTACCTCCCAGGAGGGCAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGtct                                                        >  1:2837647/1‑150 (MQ=255)
                                                                                                aCGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATGTTTTTGTCCCACTAGTATAATTTTTTACCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTTGGCATGTTGtctc                                                       >  1:8179954/1‑150 (MQ=255)
                                                                                                aCGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTGGGATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGtctc                                                       <  1:9358479/150‑1 (MQ=255)
                                                                                                 cGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCGGAGTTTTATCTTTTTTTCCCACTAGAATCAATTTTTGCCTCCCTGGAGGACAAACGTGTCTAAGCGGCGTGTAGTTGTGACAGGGCTGGGCATGTTGtctac                                                      >  1:10489025/1‑148 (MQ=255)
                                                                                                  ggCCACCAGGCGTAAGTGAACATCTCCCGGCGGTCGTTCGACCGCCTGAGGGGGATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCt                                                     <  1:7018/150‑1 (MQ=255)
                                                                                                  ggCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACGAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGGCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCt                                                     >  1:8851384/1‑150 (MQ=255)
                                                                                                  ggCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTCTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCt                                                     <  1:14438034/150‑1 (MQ=255)
                                                                                                     caccaGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTc                                                  <  2:11602729/150‑1 (MQ=255)
                                                                                                     caccaGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACAAGAATCATTTTTTACCACCCGTGAGGACAAACGTGTCTAAGCGTAGTGTAGTTGTGACCGGACTGGGCCTGTTGTCTCCTGTc                                                  >  1:12070819/1‑150 (MQ=255)
                                                                                                      accaGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCg                                                 <  2:10567892/150‑1 (MQ=255)
                                                                                                      accaGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCGGAGTTTTATATTTTTGTCCCACTAGAATAATTTTTTAACACCCAGGTGGACAAACGTGTCTAAGCGGCGTGTAGTTGTGTCCGGACATGGCAGGTTGTATCCTGTCg                                                 >  1:3900130/1‑150 (MQ=255)
                                                                                                       ccaGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTGGGATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCgg                                                <  1:15417107/150‑1 (MQ=255)
                                                                                                        caGGCGTTAGTGACCATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACACACGTGTCTTAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGc                                               <  2:9613539/150‑1 (MQ=255)
                                                                                                              tAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATAcc                                         <  2:14477946/150‑1 (MQ=255)
                                                                                                               aaGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCg                                        <  2:8854623/150‑1 (MQ=255)
                                                                                                                 gTGAACATCTCCCGGCGGTCGTTCGACCGCCTGAGGGGGAGCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTa                                      <  1:5885823/150‑1 (MQ=255)
                                                                                                                   gAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTaga                                    <  1:7329876/150‑1 (MQ=255)
                                                                                                                       aTCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCt                                >  2:12090/1‑150 (MQ=255)
                                                                                                                        tctcCTGGCGGTCGTTCGACCGCCTGATTTTTATCTTTTTGTCCCACTAGATTCATTTTTTCCCTCCCTGGAGGACAATCGTGTCTAAGCTTCGTGTTGTTGTGACCTGACTGGGGATGTTGTCTACTGTCGGCAATACCGTAGAGTCTa                               <  2:7500585/150‑1 (MQ=255)
                                                                                                                        tctcCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTa                               <  2:4421591/150‑1 (MQ=255)
                                                                                                                            cAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTTTCCCACTAGAATCATTTTTTACCACCCAGGAGGACAAAAGAGTCTAAGCGTCGTGTAGTTTTGACCGGAATGGGCATGTTGTCTACTGTCGGCAATAACCTAGAGTCTAccag                           >  1:9746323/1‑148 (MQ=255)
                                                                                                                            cAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGGGTCTAAGCGTCGTGTAGTGGTGACCGGACTGGGCAGGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTg                           >  2:14712298/1‑150 (MQ=255)
                                                                                                                              ggCGGTCGAACGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGa                         >  1:3449558/1‑150 (MQ=255)
                                                                                                                              ggCGGTCGAACGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGACAATACCGTAGAGTCTACCTGGa                         >  2:1984677/1‑150 (MQ=255)
                                                                                                                                  gTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTa                                                                                   >  2:4205090/1‑88 (MQ=255)
                                                                                                                                    cGTTCGACCGCCTGAGTGGGATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGctc                   <  1:2466660/150‑1 (MQ=255)
                                                                                                                                      ttCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTg                 >  1:5136955/1‑150 (MQ=255)
                                                                                                                                      ttCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTg                 <  2:3583032/150‑1 (MQ=255)
                                                                                                                                        cGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCt               >  1:848452/1‑150 (MQ=255)
                                                                                                                                        cGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCt               <  2:1539160/150‑1 (MQ=255)
                                                                                                                                         gACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCtt              >  2:11959797/1‑150 (MQ=255)
                                                                                                                                            cGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGcc           <  1:12836894/150‑1 (MQ=255)
                                                                                                                                             gCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTACCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCg          <  2:10163220/150‑1 (MQ=255)
                                                                                                                                                   gTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCaga    <  2:3900130/150‑1 (MQ=255)
                                                                                                                                                    ttttATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCAGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCagag   >  1:11778295/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGT  >  NC_000913/1151739‑1152037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: