Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 151,688 C→G 9.3% V182L (GTA→CTA)  yadL ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913151,6880CG 9.3% 2494.5 / 35.5 793V182L (GTA→CTA) yadLputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (436/265);  new base G (2/86);  total (439/354)
Fisher's exact test for biased strand distribution p-value = 1.18e-30
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 811 total aligned reads displayed.

AAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGA  >  NC_000913/151539‑151837
                                                                                                                                                     |                                                                                                                                                     
aaaaCAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTAc                                                                                                                                                       >  2:5071043/1‑150 (MQ=255)
aaaaCAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTAc                                                                                                                                                       >  1:8078994/1‑150 (MQ=255)
 aaaCAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACg                                                                                                                                                      <  1:3299643/150‑1 (MQ=255)
   aCAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGt                                                                                                                                                    <  2:6041248/150‑1 (MQ=255)
     aGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGttt                                                                                                                                                  <  2:2173656/150‑1 (MQ=255)
       tAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTa                                                                                                                                                <  2:6664098/150‑1 (MQ=255)
        aaTGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTAt                                                                                                                                               <  2:10448328/150‑1 (MQ=255)
            acagaaacagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTAttttt                                                                                                                                           <  1:948961/150‑1 (MQ=255)
            acagaaacagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTAttttt                                                                                                                                           >  1:4705731/1‑150 (MQ=255)
             cagaaacagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTAtttttt                                                                                                                                          <  2:3669240/150‑1 (MQ=255)
              agaaacagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTGAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTAttttttt                                                                                                                                         >  2:2912755/1‑150 (MQ=255)
              agaaacagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTAttttttt                                                                                                                                         <  2:7634812/150‑1 (MQ=255)
              agaaacagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTAttttttt                                                                                                                                         <  1:1963942/150‑1 (MQ=255)
              agaaacagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTAttttttt                                                                                                                                         >  1:12048000/1‑150 (MQ=255)
              agaaacagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACAGTGTTTACGGTTTTAttttttt                                                                                                                                         >  2:10691892/1‑150 (MQ=255)
               gaaacagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTc                                                                                                                                        <  1:13733141/150‑1 (MQ=255)
                   cagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACg                                                                                                                                    <  2:8710039/150‑1 (MQ=255)
                    agaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGa                                                                                                                                   <  2:1300123/150‑1 (MQ=255)
                    agaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGa                                                                                                                                   >  1:6545120/1‑150 (MQ=255)
                      aaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGAcc                                                                                                                                 <  2:8423063/150‑1 (MQ=255)
                        ttattCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTAGAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAg                                                                                                                               >  1:15491360/1‑150 (MQ=255)
                          attCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGc                                                                                                                             <  2:12628050/150‑1 (MQ=255)
                          attCTCGTCATCAGGTTACGTTGAGTGGGATGCTTATTTAGTTCCTGATTCTGTTAGTCTGTTAGTTATTGGTTACTGTAATTGTGGCTGGTGCAGAAATCTTACCCGCAGTGACTTTGTTTACGTTTTTATTTTTTTCCACGACCAGGc                                                                                                                             <  1:14374854/150‑1 (MQ=255)
                           ttCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCg                                                                                                                            >  2:6136542/1‑150 (MQ=255)
                           ttCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCg                                                                                                                            >  2:6877249/1‑150 (MQ=255)
                              tctTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGc                                                                                                                         >  1:8578949/1‑150 (MQ=255)
                              tctTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGc                                                                                                                         >  1:10037569/1‑150 (MQ=255)
                              tctTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGc                                                                                                                         >  2:1188175/1‑150 (MQ=255)
                                   atcaGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCa                                                                                                                    >  2:7880716/1‑150 (MQ=255)
                                     caGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATc                                                                                                                  <  1:756874/150‑1 (MQ=255)
                                        cTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTAGTGTAAATGTGGCTGGTGCAGAAATCTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGa                                                                                                               >  1:10880826/1‑150 (MQ=255)
                                         ttACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCggtg                                                                                                              >  2:970/1‑148 (MQ=255)
                                         ttACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGat                                                                                                              >  1:7330919/1‑150 (MQ=255)
                                         ttACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGat                                                                                                              >  1:132185/1‑150 (MQ=255)
                                             gTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGataata                                                                                                          >  2:13708492/1‑150 (MQ=255)
                                             gTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGataata                                                                                                          >  2:1340748/1‑150 (MQ=255)
                                             gTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAACGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGataata                                                                                                          >  1:4054206/1‑150 (MQ=255)
                                              ttGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGAtaataa                                                                                                         <  1:4353802/150‑1 (MQ=255)
                                               tGAGTGGGATGCATCTCTAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAATTGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATaaa                                                                                                        <  2:13205636/150‑1 (MQ=255)
                                               tGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATaaa                                                                                                        <  1:2682852/150‑1 (MQ=255)
                                               tGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTAGAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATaaa                                                                                                        <  1:6045483/150‑1 (MQ=255)
                                                gAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAAc                                                                                                       >  2:2418356/1‑150 (MQ=255)
                                                gAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAAc                                                                                                       <  1:8735692/150‑1 (MQ=255)
                                                     ttttGCATATTTTTTTCGTTAATCTGTATGTCTTTTTTTTATAGTTTACTGTATTTGTGGCTGGTGCATAAAACTTACCCGCTGTGACTTTTTTTAGGTTTTTATTTTTTCCCACGACCAGGCGTGCACTCTTCGGATAATAAACGACCg                                                                                                  <  2:8197996/147‑1 (MQ=255)
                                                     ggATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCg                                                                                                  >  1:13949183/1‑150 (MQ=255)
                                                           atatatAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTgg                                                                                            >  1:5301093/1‑150 (MQ=255)
                                                           atatatAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTgg                                                                                            >  2:5842809/1‑150 (MQ=255)
                                                                 aGTTCCTCAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAg                                                                                      >  2:2661547/1‑150 (MQ=255)
                                                                  gTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGt                                                                                     >  2:4378034/1‑150 (MQ=255)
                                                                   ttCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGGAGAAAACTTACCCGCAGTGAGATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCAGTCATCTGATAATAATCGACCGCTTTGGCTGCAGTg                                                                                    >  2:14889140/1‑150 (MQ=255)
                                                                     ccTGAATCTGTATGTCTGTTAGTTTTAGTTTACTGTAATTGTGGCTGGTGCAGAAAACTTACCCGCTGTTACATTGTTTACGTTTTTATTTTTTTCCACGACCCGGCGTGCACTCATCGTATAATAAACGACCGCATTGGCTGCAGTGga                                                                                  <  2:3244914/150‑1 (MQ=255)
                                                                     catGAATCTTTAAGTCTTTTAGTTATAGTTTACTGTAATTGTGGATGGTGAAGAAAACTTACCCGAATTGACTTTTTTTACTTTTTTTTTTTTTTCCACGACAAGGCGTGCACTCATCTGATAATAAACGACCGCATTGGCTGCAGTGga                                                                                  <  2:11322800/148‑1 (MQ=255)
                                                                      cTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAATCTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTggtg                                                                                 >  2:7226311/1‑148 (MQ=255)
                                                                      cTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGAACAGGCGTGCACTCATCGGATAATAAAAGACCGCATTGGCTGAAGTGgag                                                                                 >  2:10888922/1‑150 (MQ=255)
                                                                       tGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAATTGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTACACGACCAGGCGTGCACTCATCGGTTAATAAACGACCGCATTGGCTGCAGTGgaga                                                                                >  1:12569832/1‑150 (MQ=255)
                                                                        gAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGAt                                                                               >  1:564147/1‑150 (MQ=255)
                                                                         aaTCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGAtt                                                                              <  2:3922930/150‑1 (MQ=255)
                                                                              gTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTAc                                                                         >  2:740352/1‑150 (MQ=255)
                                                                                   tCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCtt                                                                    >  1:6254522/1‑150 (MQ=255)
                                                                                   tCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCtt                                                                    >  2:1769589/1‑150 (MQ=255)
                                                                                   tCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTACCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCtt                                                                    <  2:4768444/150‑1 (MQ=255)
                                                                                    gtgtttgttATCGGTTACTGTAAATGTGGGTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTAGGTTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTtg                                                                   <  2:15312480/149‑1 (MQ=255)
                                                                                    gtGTTAGTTATCGGTTACTGTAATTGGGGGTGGTGCAGAAAACTTACCCGCCGTGACTTTGTTTCGGTTTTTATTTTTTTCCACGACCCGGCGGGCACCCATCGGATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTtg                                                                   <  2:12247466/149‑1 (MQ=255)
                                                                                      gttagttaTAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTtgtg                                                                 >  1:10368587/1‑150 (MQ=255)
                                                                                           ttaTAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCt                                                            >  1:5060773/1‑150 (MQ=255)
                                                                                           ttaTAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCt                                                            >  1:11522436/1‑150 (MQ=255)
                                                                                           ttaTAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGAGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTCCAGTGGAGATTGTTACCTCTTGTGATGCt                                                            >  2:9250021/1‑150 (MQ=255)
                                                                                             tttgtttACTGTAATTGTGGCTGGTGAATAAAACTAACCCGCTGTTACTTTTTTTTCGTTTTTATTTTTTTCCACGACCTGGCGTGCACACATCGTATAAAAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTgg                                                          <  1:8323354/145‑1 (MQ=255)
                                                                                             ataGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTgg                                                          >  2:14494582/1‑150 (MQ=255)
                                                                                                 gTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGtt                                                      >  1:14942448/1‑150 (MQ=255)
                                                                                                      tGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTAcaca                                                 >  2:7989254/1‑150 (MQ=255)
                                                                                                        tAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGGGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGc                                               >  1:7853444/1‑150 (MQ=255)
                                                                                                             gtgGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAAt                                          >  1:3039428/1‑150 (MQ=255)
                                                                                                                gCTGGTGCATAAAACTTACCCGCTGTGACATTTTTTACGGTTTTATTTTTTCCCACGCCCTGGCGTGCACTCATGGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATtgt                                       <  1:15258392/150‑1 (MQ=255)
                                                                                                                gCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATtgt                                       >  2:12011213/1‑150 (MQ=255)
                                                                                                                gCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATtgt                                       >  2:15527054/1‑150 (MQ=255)
                                                                                                                 cTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATtgtg                                      <  2:14088825/150‑1 (MQ=255)
                                                                                                                  tGGTGCAGAAAACTTACCCGCAGTTACTTTTTTTAGGTTTTTATTTTTTCCCACGACCAGGCGTGCACTCATCGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTgtgt                                     <  1:9996358/150‑1 (MQ=255)
                                                                                                                   ggTGCATAAAACTTACCCGCTGTGACTTTGTTTTGGTTTTTATTTTTTTCCACGACCAGGGGTGCACTCTTGGTATAAAAAACGACCGCATTGGCTGCGGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGtt                                    <  1:9753461/150‑1 (MQ=255)
                                                                                                                   ggTGCAGAAAACTTCCCCGCTGTGACTTTGTTTAGGGTTTTTTTTTTTTCCCCGACCGGGGGTGCACTCCGCGGATACTACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGtt                                    <  1:11746443/150‑1 (MQ=255)
                                                                                                                      gCATAAAACTTCCCCGCAGTGACATTGTTTCCGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCa                                 <  2:12481982/150‑1 (MQ=255)
                                                                                                                        aGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACt                               >  2:1941023/1‑150 (MQ=255)
                                                                                                                         gAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTc                              >  1:6883034/1‑150 (MQ=255)
                                                                                                                             aCTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTg                          >  1:9141127/1‑150 (MQ=255)
                                                                                                                               ttACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGtt                        >  2:13934143/1‑150 (MQ=255)
                                                                                                                                  cccGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGGACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGcc                     >  1:13487001/1‑150 (MQ=255)
                                                                                                                                   ccGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCg                    >  2:11702840/1‑150 (MQ=255)
                                                                                                                                   ccGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCg                    >  2:4159932/1‑150 (MQ=255)
                                                                                                                                   ccGAAGTTACATTTTTTACTTTTTAATTTTTTACCACCACCAGGCTTGCAAAAATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCg                    <  1:1739382/150‑1 (MQ=255)
                                                                                                                                     gCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGa                  >  1:2200890/1‑150 (MQ=255)
                                                                                                                                        gTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGtt               >  1:3640797/1‑150 (MQ=255)
                                                                                                                                         tGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTa              <  2:10231285/150‑1 (MQ=255)
                                                                                                                                          taaaTTGTTTAGGGTTTTATTTTTTCCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTAc             <  2:6344305/147‑1 (MQ=255)
                                                                                                                                           actttttttAGGTTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACa            <  2:5618898/144‑1 (MQ=255)
                                                                                                                                               tGTTTACGGTTTTATTTTTTTCCACGACCCGGCGTGCACTCATCGGATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGc        <  1:11440413/150‑1 (MQ=255)
                                                                                                                                                gTTTAGTGTTTTATTTTTTCCCACTACCCGGCGGGCACCCATCGTTTAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCt       <  2:5309107/150‑1 (MQ=255)
                                                                                                                                                 tttACGGTTTTAGTTATTTCCCCGACCAGGCGTGCACTCAGCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTc      <  1:8441563/150‑1 (MQ=255)
                                                                                                                                                  ttACGGTTTTATTTTTTCCCACGACCCGGCTTGCACTAATCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTcc     <  1:5798377/150‑1 (MQ=255)
                                                                                                                                                   tACGGTTTTATTTTTGTCCACGACCAGGCGTGCACTCCTCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCa    <  2:9020187/150‑1 (MQ=255)
                                                                                                                                                    aCGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAg   >  2:9430126/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGA  >  NC_000913/151539‑151837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: