Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,164,546 A→T 13.7% D151V (GAT→GTT)  nagZ → beta N‑acetyl‑glucosaminidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,164,5460AT13.7% 2046.5 / 81.8 733D151V (GAT→GTT) nagZbeta N‑acetyl‑glucosaminidase
Reads supporting (aligned to +/- strand):  ref base A (409/202);  new base T (2/117);  total (411/322)
Fisher's exact test for biased strand distribution p-value = 1.40e-45
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 759 total aligned reads displayed.

TTGGTTGATGGCCAGCGAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTAA  >  NC_000913/1164397‑1164695
                                                                                                                                                     |                                                                                                                                                     
 tggttgATGGCCAGCGAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGAt                                                                                                                                                      <  1:8806358/150‑1 (MQ=255)
  ggttgATGGCCAGCGAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATg                                                                                                                                                     <  1:14674524/150‑1 (MQ=255)
  ggttgATGGCCAGCGAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATg                                                                                                                                                     <  2:7940620/150‑1 (MQ=255)
   gttgATGGCCAGCGAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATgg                                                                                                                                                    <  2:11388996/150‑1 (MQ=255)
   gttgATGGCCAGCGAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTAAACATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATgt                                                                                                                                                    >  1:14080334/1‑149 (MQ=255)
    ttgATGACCAGCGAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGt                                                                                                                                                   <  1:2201054/150‑1 (MQ=255)
    atgATGGCCAGCGAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGt                                                                                                                                                   >  1:8481695/2‑150 (MQ=255)
     tgATGGCCAGCGAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTa                                                                                                                                                  <  2:13802111/150‑1 (MQ=255)
        tGGCCAGCGAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGc                                                                                                                                               <  2:14437269/150‑1 (MQ=255)
         ggCCAGCGAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCa                                                                                                                                              >  1:3279060/1‑150 (MQ=255)
          gCCAGCGAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCAt                                                                                                                                             >  2:1338595/1‑150 (MQ=255)
           ccAGCGAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATg                                                                                                                                            <  1:4633996/150‑1 (MQ=255)
                gAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGcc                                                                                                                                       <  1:6455156/150‑1 (MQ=255)
                gAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGcc                                                                                                                                       >  1:11605500/1‑150 (MQ=255)
                gAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGcc                                                                                                                                       <  1:1012333/150‑1 (MQ=255)
                 aaaTGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCg                                                                                                                                      <  1:1661493/150‑1 (MQ=255)
                 aaaTGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCg                                                                                                                                      <  1:10510349/150‑1 (MQ=255)
                   aTGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGa                                                                                                                                    <  2:8444288/150‑1 (MQ=255)
                    tGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGaa                                                                                                                                   >  1:8222972/1‑150 (MQ=255)
                    tGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGaa                                                                                                                                   <  1:14882001/150‑1 (MQ=255)
                          cTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGaaaa                                                                                                                             <  2:14836226/150‑1 (MQ=255)
                              ggATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGAc                                                                                                                         >  2:6491611/1‑150 (MQ=255)
                                  atTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAATGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCggg                                                                                                                     >  1:1864997/1‑150 (MQ=255)
                                   ttGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGa                                                                                                                    >  1:6966014/1‑150 (MQ=255)
                                       tatCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAaca                                                                                                                >  1:14302593/1‑150 (MQ=255)
                                        atCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTAGTGATGGTATGCATGAAGCCGGACTGAAAACGACCGGGAAacac                                                                                                               >  2:1677565/1‑150 (MQ=255)
                                           aGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTc                                                                                                            >  2:3119098/1‑150 (MQ=255)
                                           aGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTc                                                                                                            >  2:11148240/1‑150 (MQ=255)
                                              tttGCGCCTGTGCTGTATGTCGGGCATATCAGCGCGGCTATTGGGGAGCGTTATTATCATGCCGATCCAAAAAAAGCCCTGGAAATTGCCAGCCGTTTTATTGTTGGTATGCATGAAGCCGTAATGAAAACGACCGGGAAACACTTCCCa                                                                                                         <  2:9603691/150‑1 (MQ=255)
                                              tttGCGCCTGTGCTGGTGGTCGGGCATATCAGGGCGGCGTTTGGCGTGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGTTATGCATGAAGCGGTAATGAACACGACCGGGAAACACTTCCCa                                                                                                         <  2:9106031/150‑1 (MQ=255)
                                              tttGCGCCGGGGCGGGTTGTCGGGCATACCCGCGCGGCGATGGGCGTGCTTTCTTATCATGCCGATCAAAAAAAAGCCCTGGCATTGGCCCGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCa                                                                                                         <  2:7603215/150‑1 (MQ=255)
                                                      tgtgCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGTTGGTATGCATGTAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACgg                                                                                                 >  1:7404194/1‑150 (MQ=255)
                                                        tggtgtttGTCGGGCATAACCGCGGGGCGTTGGGCGTGCTTTCTTACCCTGCCTAACCACAAAAACCCCTGCCATTTGCCAGCGGTTTTTTTGTTGGTATGAATGAAGCCGTAATTAACACGACCGGGAAACACTTCCCAGGACACGGTg                                                                                               <  2:4106779/143‑1 (MQ=255)
                                                        tgCTGGATGTGGGGCATATCAGCGCGGCTATTGGCGTGCGTTCTTATCATGCCGATCCACAAAAAGCCCGGGCAAGTGCCCGCGGTTTTTTTGTTGGTTTGCATGAACCCGTTTTCAAAACGACCGGGAAACACTTCCCAGGACACGGTg                                                                                               <  2:11895779/150‑1 (MQ=255)
                                                          cTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCa                                                                                             >  2:10941931/1‑150 (MQ=255)
                                                           tGGATGTCGGGCATATCCGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGACCCACAAAAAGCCCGGGCAATTGCCAGCCGGTTTATTGTTGTTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAg                                                                                            <  2:10007328/150‑1 (MQ=255)
                                                             gATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTa                                                                                          >  2:2929080/1‑150 (MQ=255)
                                                             gATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTa                                                                                          >  2:4709731/1‑150 (MQ=255)
                                                                  ggggCATATCGGCGGGGGGTTTGGCGTGGGTTTTTATCATGCCGAACCACAAAACGCCCTGGCATTGGCCCGCGGTTTTTTTTTTGTTATCCATGACGCGGGACTCAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGc                                                                                     <  2:6857738/149‑1 (MQ=255)
                                                                  cGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGc                                                                                     >  1:3436436/1‑150 (MQ=255)
                                                                    ggCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAg                                                                                   >  1:5426111/1‑150 (MQ=255)
                                                                     gCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGa                                                                                  >  2:695289/1‑150 (MQ=255)
                                                                      cATAACCGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAACAGCCCGGGCAATGGCCCGCCGGTTTATTGTTGGTTTGCATTAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGAc                                                                                 <  2:9783613/150‑1 (MQ=255)
                                                                            aGCGCGGCGATTGGCGAGCGTTCTTATCACGCCGATCCACAAAAAGCCCTGGCCATTGCCAGCCGGGTTATGGAGGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTcacac                                                                           >  1:7866989/1‑150 (MQ=255)
                                                                             gcgcGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCCGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTcacaca                                                                          >  1:11095306/1‑150 (MQ=255)
                                                                               gcggcgATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGTTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACaaa                                                                        >  1:5968312/1‑150 (MQ=255)
                                                                               gcggcgATTGGCGAGCGTTCTTATCATGCCGATCCAAAAAACGCCCGGGAAATGGCCAGCGGGTTTTTTGTTGGTTTGCTTGACGCCGGAATGAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACaaa                                                                        <  2:10827401/150‑1 (MQ=255)
                                                                                 ggcttttGGCTAGCTTTCTTATCATGCCTATACAAAAAAAGCCCTGGCAATTGCCAGCCGTTTTATTGATGTTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGa                                                                      <  2:10153064/145‑1 (MQ=255)
                                                                                 ggcgATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGa                                                                      >  2:1459028/1‑150 (MQ=255)
                                                                                 ggcgATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGa                                                                      >  1:2994088/1‑150 (MQ=255)
                                                                                   cttttGGCGTGCTTTTTTATCATGCCTATCCAAAAAAAGCCCTGGATTTTGCCTGCGGTTTTTTTTTTGGTTTGAATTAAGCCGTAATGAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGaaa                                                                    <  2:3640688/147‑1 (MQ=255)
                                                                                   cttttGGCGAGCTTTCTTATCAGGCCGATCAAAAAAAAGCCCTGGAATTTGCCAGCGGTTTTATTGTTGGTATGCATGAAGCCGTAATGAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGaaa                                                                    <  2:6052609/147‑1 (MQ=255)
                                                                                      ttGGCGAGCGTTATTATCTTGCCTATCCAAAAAAAGCCCTGGAATTTGCCTGCGGTTTTTTTGATGTTATGAATTAAGCCGCAATTAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAacac                                                                 <  2:487799/150‑1 (MQ=255)
                                                                                       tGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACAcc                                                                >  1:13474921/1‑150 (MQ=255)
                                                                                         gCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGt                                                              >  2:15320474/1‑150 (MQ=255)
                                                                                          cGAGCTTTTTTATCTGGCCGACCCACAAAAAGCCCTGCAACTTGCCCGCTGGTTTTTGTTTGGTATGCTTGACGCGGCACTTAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTg                                                             <  2:5311138/150‑1 (MQ=255)
                                                                                              gtttattATCATGCCGATCAAAAAAAAGCCCTGGAATTTGCCAGCGGTTTTTTTTTTGGTTTGCTTGAAGCCGGAATTAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGAt                                                         <  2:12716227/145‑1 (MQ=255)
                                                                                               tttCTTATCATGCCGAACAAAAAAAAGCCCTGGAACTTGCCAGCGTTTTTTTTGTTGGTATGCATGAAGCCGTAATTAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATc                                                        <  1:4116846/149‑1 (MQ=255)
                                                                                               gTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATc                                                        >  1:11240675/1‑150 (MQ=255)
                                                                                               gTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATc                                                        >  1:9448067/1‑150 (MQ=255)
                                                                                                 tattATCATGCCGATCAAAAAAAAGCCCTGTAATTTGCCAGCGGTTTTTTTTTTGGTTTGCATTAAGCCGTATTTAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCa                                                      <  2:9480230/148‑1 (MQ=255)
                                                                                                  tttATCATGCCTAACCAAAAAAAGCCCTGGAATTTGCCTGCTGTTTTTTTGTTGTTATTCTTTACGCCGGAATCAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCAc                                                     <  1:15472461/149‑1 (MQ=255)
                                                                                                  attATCTTGCCGATCCAAAAAACGCCCTGGAAATGGCCTGCCGGTTTATTTTTGGTTTGCATGAAGCGGTAATCAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCAc                                                     <  1:9066247/149‑1 (MQ=255)
                                                                                                  attATCCTGCCCATCCACAAAATGCCCTGGCAATTGCCAGCGGTTTTATTGTTGTTCTGCATGATGCCGGAATCAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCAc                                                     <  2:12334174/149‑1 (MQ=255)
                                                                                                   ttATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACg                                                    >  2:8120909/1‑150 (MQ=255)
                                                                                                    tATCATGCCGATCCACAAAATGCCCTGGAATTTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGt                                                   <  2:11711468/150‑1 (MQ=255)
                                                                                                        aTGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCcac                                               >  2:10405380/1‑150 (MQ=255)
                                                                                                         tGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCcaca                                              >  2:3958785/1‑150 (MQ=255)
                                                                                                          gCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACaa                                             >  1:12679457/1‑150 (MQ=255)
                                                                                                             gATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCg                                          >  1:12048885/1‑150 (MQ=255)
                                                                                                              aacaacaAAAACCCCTGCAAATGGCCAGCGGTTTTTTTGTTGTTATGCATTACGCCGTAATTAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCgg                                         <  1:6247771/146‑1 (MQ=255)
                                                                                                              aTCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCgg                                         >  1:1523616/1‑150 (MQ=255)
                                                                                                                 cacaAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGgaga                                      >  1:12994729/1‑150 (MQ=255)
                                                                                                                 cacaAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGgaga                                      >  2:896849/1‑150 (MQ=255)
                                                                                                                 caaaaaacGCCCTGGCAATGGCCAGCGGTTTTTTTGAGGGTTGGCATGACGCCGTATTTAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGgaga                                      <  2:15003603/147‑1 (MQ=255)
                                                                                                                  acaAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGAt                                     >  1:7070137/1‑150 (MQ=255)
                                                                                                                   caAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGAtt                                    >  1:960583/1‑150 (MQ=255)
                                                                                                                        aGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGaa                                                                                                                               >  2:5807036/1‑54 (MQ=255)
                                                                                                                           ccgggCAATGGCCAGCCGGTTTATTGATGGTATGCATGACGCCGTAATGAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTaa                            <  2:7422468/147‑1 (MQ=255)
                                                                                                                            cTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTaaa                           >  2:4492742/1‑150 (MQ=255)
                                                                                                                             tGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAg                          >  2:1304832/1‑150 (MQ=255)
                                                                                                                              ggCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGACCACAAGCGGAGATTCGCGCTAAAGa                         >  1:13749773/1‑150 (MQ=255)
                                                                                                                               gCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGat                        >  2:2642885/1‑150 (MQ=255)
                                                                                                                               gCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGat                        >  2:1847105/1‑150 (MQ=255)
                                                                                                                                cAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGata                       >  2:14217483/1‑150 (MQ=255)
                                                                                                                                       cAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGt                >  1:3887551/1‑150 (MQ=255)
                                                                                                                                        aGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTc               >  1:10829040/1‑150 (MQ=255)
                                                                                                                                          ccGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCtt             >  1:3768984/1‑150 (MQ=255)
                                                                                                                                           cgttttATTGATGGTATGAATGAAGCCGGAATTAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTc            <  2:6262844/147‑1 (MQ=255)
                                                                                                                                           cGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTc            >  1:2600663/1‑150 (MQ=255)
                                                                                                                                            ggTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCa           <  2:2498564/150‑1 (MQ=255)
                                                                                                                                             gTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAg          >  1:10650348/1‑150 (MQ=255)
                                                                                                                                               tttttgttgGTATGCATGTTGCCGGTTTTATAACGACCTGTAAACACTTCCGATGACACGGTGCAGTAACGGCAGACTCACACTAAGAATAACCTTGCGATCCACGTCCACAAGCTTTTATTCTCGGTAAAGATATGTCGGTATTCAGtt        <  2:3322638/147‑1 (MQ=255)
                                                                                                                                               ttATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGtt        >  2:15420321/1‑150 (MQ=255)
                                                                                                                                                tatttattGTATCCATTAAGCCGTAATTAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTc       <  2:2150838/150‑1 (MQ=255)
                                                                                                                                                 tttGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTcc      <  2:14036119/149‑1 (MQ=255)
                                                                                                                                                  ttGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCt     >  1:5058747/1‑150 (MQ=255)
                                                                                                                                                  ttGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCt     >  1:10246652/1‑150 (MQ=255)
                                                                                                                                                   tGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCtt    >  1:4423481/1‑150 (MQ=255)
                                                                                                                                                    gATGGTTTGCATTACGCCGGAATGAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTa   <  2:10618738/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTGGTTGATGGCCAGCGAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTAA  >  NC_000913/1164397‑1164695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: