Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,133 T→A 5.6% noncoding (104/171 nt)
E15V (GAG→GTG) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,1330TA 5.6% 1662.5 / 11.0 610noncoding (104/171 nt)
E15V (GAG→GTG) 
rttR
tpr
rtT sRNA, processed from tyrT transcript
protamine‑like protein
Reads supporting (aligned to +/- strand):  ref base T (254/283);  new base A (51/3);  total (324/286)
Fisher's exact test for biased strand distribution p-value = 1.46e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 634 total aligned reads displayed.

GTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGA  >  NC_000913/1286984‑1287282
                                                                                                                                                     |                                                                                                                                                     
   gAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAATCAGCGCTGCGCGCATCGCCCATCGGGTCGTTGCCTGCGGCAACGctctc                                                                                                                                                    >  1:12926228/1‑150 (MQ=255)
   gAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTGGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGGTGCCTGCGGCAACGctctc                                                                                                                                                    >  2:7420961/1‑150 (MQ=255)
     aGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCtctctc                                                                                                                                                  >  2:2862746/1‑150 (MQ=255)
             aTCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGgcgc                                                                                                                                          >  1:1966906/1‑150 (MQ=255)
              tCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGGCGGCGGCAACGCTCACAAGCTGGCGCt                                                                                                                                         >  1:6656590/1‑150 (MQ=14)
               ccgtCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGCCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTc                                                                                                                                        <  2:9413062/147‑1 (MQ=255)
               ccTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTc                                                                                                                                        <  2:14923870/150‑1 (MQ=255)
                 ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGAGGCAAAGCTCTCTCGCTGgcgcgaga                                                                                                                                      >  1:5033118/1‑146 (MQ=255)
                 ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                      >  2:6835289/1‑150 (MQ=255)
                 ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                      >  1:12540333/1‑150 (MQ=255)
                     ccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCg                                                                                                                                  >  2:5081469/1‑150 (MQ=255)
                     ccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGGCg                                                                                                                                  >  1:4803634/1‑150 (MQ=255)
                     ccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCATCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCg                                                                                                                                  >  1:6400771/1‑150 (MQ=255)
                        cATGGGCAGAATAGTTCACTGAGGATTCGCTTGAGAGTTCAGGGCCTTTTGCAAGTGATGGTGGTGGGGGAAGGATTACTCCGCGCTGCGCGCTTCCCCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAAc                                                                                                                               <  1:146545/150‑1 (MQ=14)
                              cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGGAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                                                                                                                         >  2:8698263/1‑150 (MQ=255)
                              cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                                                                                                                         <  2:12540333/150‑1 (MQ=255)
                              cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                                                                                                                         <  1:11296257/150‑1 (MQ=255)
                                   tatTTGATTTCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAg                                                                                                                    <  2:8223170/150‑1 (MQ=255)
                                   tatTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACACACGCTGGCGCTCGAGTCGAACCGTTGTCGaaa                                                                                                                    >  1:1685184/1‑149 (MQ=14)
                                     tttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCACTCACGCAGGCGCACGAGTCGAACCATGGTCGAAGcg                                                                                                                  >  2:5279323/1‑149 (MQ=14)
                                       tGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtc                                                                                                                <  2:3553072/150‑1 (MQ=255)
                                        gATTGCGGATTCGATTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtct                                                                                                               <  2:11697821/150‑1 (MQ=255)
                                             cGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATcc                                                                                                          <  1:7796623/150‑1 (MQ=255)
                                              ggATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCCACGCTCTCACGCTGGCGCTCGAGTGGTACCGTGGTCGAAGCGTCTCATCCt                                                                                                         >  1:12708937/1‑150 (MQ=14)
                                              ggATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCTCTCGCTGGCGCTCGAGTAGAACCATGGTCGAAGCTTCTCATACt                                                                                                         >  2:1838089/1‑150 (MQ=255)
                                                  tCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTccc                                                                                                     >  2:13960248/1‑150 (MQ=255)
                                                    gCTTGAGAGTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCg                                                                                                   <  2:12885682/150‑1 (MQ=255)
                                                    gCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTTGTCGAAACGTCACATCCTTccccc                                                                                                   >  2:219126/1‑149 (MQ=255)
                                                    gCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCGCGACTCGAACCTTGGTCGAAGCTTCTCACCCTTCCCCg                                                                                                   >  2:7717087/1‑150 (MQ=255)
                                                      ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCa                                                                                                 <  1:8425368/150‑1 (MQ=255)
                                                      ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACCCTCACTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAAACTTCCCCCCa                                                                                                 >  1:1097179/1‑150 (MQ=14)
                                                         agatTTCCGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATgg                                                                                              <  1:3575034/150‑1 (MQ=255)
                                                          gagTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAATGATTACTCAGCGCCGCGCGCTTCGCCCTACGGGTCGTAGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                             <  2:10724281/150‑1 (MQ=255)
                                                             ttCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCACTCACGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTATAAACCATACCCGCAAGGGCAg                                                                                          >  1:12454050/1‑150 (MQ=14)
                                                             ttCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAAAGCTCTCTCGCTGGCGCTCGAGTCGAACCATGGTCGAATCGTCTCATACATCCCCCCAGGGGCac                                                                                          >  1:14287917/1‑149 (MQ=14)
                                                              tCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGa                                                                                         <  2:1354209/150‑1 (MQ=255)
                                                                aGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                      >  2:10465916/1‑103 (MQ=38)
                                                                aGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCCGCCACCCAATCACCCAGGCGCGCCACTCGAACCATGGTCGAAACATCTCAAAAATACCCGCAAGGGCAGAat                                                                                       >  1:11792646/1‑150 (MQ=9)
                                                                 gggACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAata                                                                                      <  1:5250864/150‑1 (MQ=39)
                                                                   gACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAtt                                                                                    >  2:14151537/1‑150 (MQ=38)
                                                                    tcTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAttt                                                                                   <  1:13595736/149‑1 (MQ=37)
                                                                    aCTTTTGTATGTGATGGTGGTTGGGGATGGATGACTCCGCGCGGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAttt                                                                                   <  1:624461/150‑1 (MQ=11)
                                                                       tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCAAGCAGGCGCTAGATAACAAACTTGGTCGAATCATAAAATACTTCCCCGCATGGGCAGAATATTTAAt                                                                                >  2:1711489/1‑150 (MQ=11)
                                                                       tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAAAGCTCTCTAGCATGCGCTCGAGTCGCACCTTGGTAGTAGCATCTAAAACATGCCCCCAAGGGGAGAAAAATTaaa                                                                                >  1:339087/1‑149 (MQ=2)
                                                                          gAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGc                                                                             <  1:2076373/150‑1 (MQ=39)
                                                                               tGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTGGGTGGCGGTCGAGTCGAACCTTGGTGGAAGCTTGTCATGGTTCCCCGCATGGGGAGAATATTTAATTGGGGGtt                                                                        >  2:13372939/1‑150 (MQ=11)
                                                                                gATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTc                                                                       <  1:2648828/150‑1 (MQ=37)
                                                                                gATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTc                                                                       <  1:14914937/150‑1 (MQ=37)
                                                                                       gtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCGTGCGGCAACGATCTCTCGCTTGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                <  1:8934217/150‑1 (MQ=255)
                                                                                       gtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACTCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAt                                                                                                            <  1:10354344/106‑1 (MQ=17)
                                                                                       gtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAt                                                                                                            <  1:10073955/106‑1 (MQ=32)
                                                                                       gtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGCGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAt                                                                                                            >  2:10073955/1‑106 (MQ=17)
                                                                                              tatgATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAg                                                         <  1:5475942/147‑1 (MQ=255)
                                                                                              aaGGATGCCTCAGCGCGGCGCGCTTCGCCCGTCGGGTCGGTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAg                                                         <  2:14319568/150‑1 (MQ=14)
                                                                                                      cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtttt                                                 <  1:13933507/150‑1 (MQ=255)
                                                                                                      cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGGCGAACCTTGGTCGAAGCTTCTCa                                                                                                             >  2:14550310/1‑90 (MQ=18)
                                                                                                      cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtttt                                                 <  1:3757251/150‑1 (MQ=255)
                                                                                                      cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTACCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCCTCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACgttt                                                 >  1:10649788/1‑150 (MQ=255)
                                                                                                          gcgcTGCGCGCTTCGCCCTTCGGGTCTTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCTTAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                             <  1:9387222/150‑1 (MQ=255)
                                                                                                              tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTg                                                                   <  1:7558766/124‑1 (MQ=39)
                                                                                                                cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGCACCGTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtg                                       <  1:6932889/150‑1 (MQ=255)
                                                                                                                 gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCACCGCTCTCACGCTGGCGCGCGAGTCGAACCGTGGTCGTAGCATCTCATCCATCCCCGCCGGGGGAGAATATTTAAGTGCGGCGTAGGGGGGAAGTTAAGGGACATTTTCAAGTGAtgg                                      >  2:6656584/1‑150 (MQ=9)
                                                                                                                     ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGTAAGTGAtggtggt                                  >  2:2486821/1‑150 (MQ=255)
                                                                                                                     ttCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCGTGGTCGAAGCTTCTCATACTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGCTGGGGAGTTCAGGGACTTTTTAAAGTGAtggtggt                                  >  2:3310290/1‑150 (MQ=11)
                                                                                                                      tCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGGGTCGAACCTTGGTCGAAGCTTCTAATCCTTCCCCGCATGGGCCGAATAATTAATTGCGGATTCGGGGGGCAGTTCAGGGTCTTTTGAGAGGGAtggtgggg                                 >  2:6466358/1‑148 (MQ=14)
                                                                                                                       cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggtgg                                <  1:1201571/150‑1 (MQ=255)
                                                                                                                       cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggtgg                                <  2:7964584/150‑1 (MQ=255)
                                                                                                                         cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAat                                                                                       <  1:8782029/93‑1 (MQ=18)
                                                                                                                         cccTTCGGGTCGTTGCCTGCGGCAAAGCTCACACGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCATGGGCAGAATATTTAATTTCGGATTCGTTGGGAAGGTCAGGGAGTTTTGAAAGTGATGGTGGTgggg                              >  2:10899939/1‑150 (MQ=17)
                                                                                                                           cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTAAAATACATCCCCCCATGGGCAGAATATTTAATTTCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGa                            >  2:6000091/1‑150 (MQ=17)
                                                                                                                            ttCGGGTCGTCGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCGTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                           <  1:12271169/150‑1 (MQ=255)
                                                                                                                               agagcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGa                        >  1:9844517/5‑150 (MQ=255)
                                                                                                                                ggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTTGGGGAAGGAt                       >  2:13712039/1‑150 (MQ=255)
                                                                                                                                 gTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGCGGGGGAAGGAtt                      <  1:1851566/150‑1 (MQ=255)
                                                                                                                                 gTCGTTGCCAGCGGCAACGCTCTCTCGCTGACGCTCGAGGCGAACCTTGGTCGAAGCTTCTCATACTTCCCCGCAGGGGCAGAATAATTGATTGCGGATTCGGTGGGAAGTTCAGGGACGTTTGAAAGTGATGGTGGTGGGGGAAGGAtt                      >  2:8919228/1‑150 (MQ=11)
                                                                                                                                  tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTc                     <  1:8151644/150‑1 (MQ=255)
                                                                                                                                  tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAATCTTCTCAACCTTCACCGCATGGGCAGAATATTAAATTGCGGAATCGGTTTGAAGATCAGGGTCTTGTGAAAGTGATGGTGGTTGGGGAAGGAGTc                     >  2:14739970/1‑150 (MQ=14)
                                                                                                                                  tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTc                     <  2:15573625/150‑1 (MQ=255)
                                                                                                                                  tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTc                     <  1:13204854/150‑1 (MQ=255)
                                                                                                                                  gcGTTGCCGCAGGCAACGCTCTCTCGCAGGCGCTCGAGTCGAACCATGGTCGAAGCTTCACAAACATCCCCCCAAGGGAAGAATAGTTAAATGCGGATTCGTTGGGAAGTTCAGGGACGTTTGAAAGTGATGGGGGTGGGGGAAGGAtta                     >  1:4053749/2‑149 (MQ=255)
                                                                                                                                    gTTGCCTTCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGACCCGTTGCCTAATCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGa                   <  2:11970661/150‑1 (MQ=17)
                                                                                                                                    gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTTGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAg                                                         <  1:1410130/112‑1 (MQ=255)
                                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGACCCTTGGTCGAAGCTTATCATCCTTCCCCGCATGGGCAGAATATTTAAATGCGGATTAGTTGGGGAGGTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGaa                  >  1:2347442/1‑150 (MQ=14)
                                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTggg                               >  2:15223826/1‑137 (MQ=255)
                                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCATGGTCGAAGCATCTAAACCTTCCCCGCAGGGGCAGAATATTTAATTGCGGATTCGTTTGGAAGTTCAGGGACTTTTGTAAGTGATGGTGGTGGGGGAGGGATTCGaa                  >  1:3168488/1‑150 (MQ=14)
                                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTgg                                                                  >  2:8326479/1‑102 (MQ=25)
                                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCTGCCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGaa                  <  1:575140/150‑1 (MQ=255)
                                                                                                                                      tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAc                 <  2:4082192/150‑1 (MQ=255)
                                                                                                                                        ccTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                                                                                                                         <  2:9711059/44‑1 (MQ=38)
                                                                                                                                        ccTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTATAAAACTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGTAGGATTCGAACCt               >  1:15287546/1‑150 (MQ=17)
                                                                                                                                         cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCtt              >  2:4687750/1‑150 (MQ=255)
                                                                                                                                         cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCtt              >  2:3709226/1‑150 (MQ=255)
                                                                                                                                         cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCtt              >  2:12313326/1‑150 (MQ=255)
                                                                                                                                         cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCtt              <  2:11330530/150‑1 (MQ=255)
                                                                                                                                         cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCATGGGCAGAAGATTTACTGGCGGATTCGATGGGACGGGCAGGGACTTTTGAAATTGATGGTGGGGGGGGAAGGATTCGAACCtt              >  2:9153640/1‑150 (MQ=17)
                                                                                                                                          tGCGGCAACGCTCTCGCGCTGTCGCTCGAGTCTAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc             <  1:10203260/150‑1 (MQ=255)
                                                                                                                                            cGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGa           >  2:4509414/1‑150 (MQ=255)
                                                                                                                                             ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGaa          <  1:9075593/150‑1 (MQ=255)
                                                                                                                                                  cGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGATTCTCATCCTTACCCGCATGGGCAGAATATTTAATTGCGGATTCGTTTGGAAGTTCAGTGACTGTTTAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGa     >  2:5592823/1‑150 (MQ=18)
                                                                                                                                                   gCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCATCCCCGCATGGGCAGAATATTTAATTTCGGATTAGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAt    >  1:11493801/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGA  >  NC_000913/1286984‑1287282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: