Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,662,370 A→C 5.9% K605N (AAA→AAC ynfF → S‑ and N‑oxide reductase, A subunit, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,662,3700AC 5.9% 1891.4 / 21.5 592K605N (AAA→AACynfFS‑ and N‑oxide reductase, A subunit, periplasmic
Reads supporting (aligned to +/- strand):  ref base A (434/109);  new base C (2/47);  total (436/156)
Fisher's exact test for biased strand distribution p-value = 1.29e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 616 total aligned reads displayed.

TACTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCAGATACCTG  >  NC_000913/1662221‑1662519
                                                                                                                                                     |                                                                                                                                                     
  cTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAAc                                                                                                                                                     >  2:121353/1‑150 (MQ=255)
       tGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCg                                                                                                                                                >  2:8928569/1‑150 (MQ=255)
          tGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGa                                                                                                                                             >  1:8681383/1‑150 (MQ=255)
           gAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGAt                                                                                                                                            >  1:14376604/1‑150 (MQ=255)
            aGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATc                                                                                                                                           >  1:9115569/1‑150 (MQ=255)
             gTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCt                                                                                                                                          >  1:10295668/1‑150 (MQ=255)
              tGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCtt                                                                                                                                         >  1:6439956/1‑150 (MQ=255)
               taaGTCGCAAAACGCTTTGGACCAGACGTTTATAAAACCTTTACTGTTGGTCGCAGTCAGCATGAATGTATCAAATATCTCCTTGCTAAAACGAAGTAAGTTAACCCTGAGATGCCCGACTACGAGGTGTTGAAAACGACCGGGATCttt                                                                                                                                        <  2:12707288/149‑1 (MQ=255)
                  gTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAg                                                                                                                                     >  1:11039268/1‑150 (MQ=255)
                     gCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGaaa                                                                                                                                  >  2:281334/1‑150 (MQ=255)
                      ccAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAACACGACCGGGAGCTGTAAGaaaa                                                                                                                                 >  2:13941676/1‑150 (MQ=255)
                        aaaCGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATTGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGaaaaaa                                                                                                                               >  1:13662368/1‑150 (MQ=255)
                        aaaCGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGaaaaaa                                                                                                                               >  1:1881288/1‑150 (MQ=255)
                         aaCGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAAt                                                                                                                              >  1:9774294/1‑150 (MQ=255)
                             cTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGccc                                                                                                                          <  2:9192468/150‑1 (MQ=255)
                              ttAGGCCCAGCGTTTTATCAAACCTTTACTGACGGCCGCAGTCAGCATTAATGTAACAAATATCTCCTTGCGAAAACGACGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAACACGACCGGGATCTTTAAGAAAAAATGCCCg                                                                                                                         <  2:1300218/150‑1 (MQ=255)
                              ttAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGCTGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCg                                                                                                                         >  1:12440749/1‑150 (MQ=255)
                              ttAGGACCAGACGTTTATCAAACCATTACTGAAGGTCGCAGTCAGCATGAATGGATCAAAAATATCCATGCGAAAACGAAGGAACGTAACCCTGAGATGACCGACAACGAGGAGATGAAAACGACCGGGAGCTTTAATAAAAAATGCCCg                                                                                                                         >  1:339007/1‑150 (MQ=255)
                                  gACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGgaag                                                                                                                     >  2:14866335/1‑150 (MQ=255)
                                     cAGACTTTTATAAAACATTTACTGACGGTCGAATTCAGAATTAATGGAAAAAATATCTCCATGCTAAAACGAAGGAACTTAACCCTGAGATGCCCGAATACGCGGTGTTGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAac                                                                                                                  <  2:9774294/150‑1 (MQ=255)
                                       gACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAacac                                                                                                                >  2:383424/1‑150 (MQ=255)
                                          gTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTAc                                                                                                             >  2:184242/1‑150 (MQ=255)
                                          gTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTAc                                                                                                             >  2:15184157/1‑150 (MQ=255)
                                          gTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTAc                                                                                                             >  2:8151619/1‑150 (MQ=255)
                                           tttATAAACCCTTTACTTATGGTCGCAGTCTGAATTATTGTATCAAATATCTCCAGGCTAAAACGACGGAACTTAACCCTTTTATGCCCGACTACGCGGATTTGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACg                                                                                                            <  2:5993151/150‑1 (MQ=255)
                                             taaaaaaCCTTTACTGATGGTCGCTGTCAGCATGATTGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTTATAGGCCCGACTACGAGTATTTTAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTc                                                                                                          <  2:7765050/146‑1 (MQ=255)
                                              aTCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCg                                                                                                         >  1:789377/1‑150 (MQ=255)
                                               tCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGc                                                                                                        >  2:3210567/1‑150 (MQ=255)
                                               tCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGc                                                                                                        >  2:51827/1‑150 (MQ=255)
                                               tCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGc                                                                                                        >  2:10289580/1‑150 (MQ=255)
                                               tCAAACCTTTACTGAAGGTCGCAGTCACCATGAATGGATCAAATAACTCCATGCCAAAACGAAGGAACGTAACCCTCAGAAGCCCGACAACGACGAGATGAAAACGACCGGGATCAATAAGAAAAAAAGCCCGCAacaacacaacaacgc                                                                                                        >  2:13455859/1‑145 (MQ=255)
                                                aaaaCCTTTAGTGATGTTCGCTTTAATCTTTATTGTATAAATTATCTCCTTGCTAAAACTAAGTAAGGTAACCCTGTTTTGCCGGACTAGGTGGTGATTAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCt                                                                                                       <  2:467543/149‑1 (MQ=255)
                                                 aaaCCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCtt                                                                                                      >  1:14153308/1‑150 (MQ=255)
                                                 aaaCCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCtt                                                                                                      >  2:12815320/1‑150 (MQ=255)
                                                      tttACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCcgc                                                                                                 >  2:3502279/1‑150 (MQ=255)
                                                       ttACTGATGGTCGCAGTCATCATTTTTGTAAAAAATCACCCCTTGCCAAACCGTCGGACCGTACCCGTGATTTGCCGGACTACGCTGATCTTAACACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCcgcg                                                                                                <  2:2934803/150‑1 (MQ=255)
                                                       ttACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCcgcg                                                                                                >  1:1497138/1‑150 (MQ=255)
                                                          cTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCAt                                                                                             >  2:6670411/1‑150 (MQ=255)
                                                            gAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTc                                                                                           >  1:750347/1‑150 (MQ=255)
                                                               ggTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGt                                                                                        >  1:5496414/1‑150 (MQ=255)
                                                                gTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTg                                                                                       >  1:1693779/1‑150 (MQ=255)
                                                                gTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTg                                                                                       >  2:7202409/1‑150 (MQ=255)
                                                                gTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTg                                                                                       >  1:8055801/1‑150 (MQ=255)
                                                                 tCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGa                                                                                      >  1:8540380/1‑150 (MQ=255)
                                                                 tCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGa                                                                                      >  1:2776738/1‑150 (MQ=255)
                                                                   gCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGCTGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAg                                                                                    >  2:10924008/1‑150 (MQ=255)
                                                                     aGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGAt                                                                                  >  1:4103292/1‑150 (MQ=255)
                                                                      gTCAGCATTAATGTATCAAATATCTCCATGCTAAAACGACGGAACGTAACCCTGAGTTGCCCGACTACGAGGATATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATc                                                                                 <  2:7581620/150‑1 (MQ=255)
                                                                       tCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATcc                                                                                >  2:11959883/1‑150 (MQ=255)
                                                                         agatttaaTGTATAAAATATCTCAATGCTAAAACGACGTAACTTAACCCTGAGAGGCCCGACTAGGTGGAGATGAACACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCcac                                                                              <  2:650728/144‑1 (MQ=255)
                                                                           cATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAg                                                                            >  1:3005654/1‑150 (MQ=255)
                                                                           cATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAg                                                                            >  2:3969347/1‑150 (MQ=255)
                                                                            aTGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAgg                                                                           >  1:12652982/1‑150 (MQ=255)
                                                                            aTGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAgg                                                                           >  1:10162390/1‑150 (MQ=255)
                                                                              gAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCa                                                                         >  1:5770513/1‑150 (MQ=255)
                                                                              gAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCa                                                                         >  1:10893150/1‑150 (MQ=255)
                                                                              gAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCa                                                                         >  1:4631056/1‑150 (MQ=255)
                                                                                ttttaaaaaaTATCTCCATGCTAAAACGACGTAACTTAACCCGGTGATGCCCTACTACGTGTAGATTAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCaaa                                                                       <  1:11918334/143‑1 (MQ=255)
                                                                                aTGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCaaa                                                                       >  2:11664286/1‑150 (MQ=255)
                                                                                    aTCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCg                                                                   >  2:14210703/1‑150 (MQ=255)
                                                                                     taaaaTACCTCCCTGCTAAAACTAAGGAACTTAACCCTGTTTTGCCCTACTACGTGTTTATTAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGt                                                                  <  1:2471061/148‑1 (MQ=255)
                                                                                     tCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGt                                                                  >  2:6912239/1‑150 (MQ=255)
                                                                                      aaattatCTCATTGCTAAAACTAAGTAACTTAACCCTTAGTTGCCCGACTACGTGGAGATTAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGtt                                                                 <  1:7777862/146‑1 (MQ=255)
                                                                                      aaaaTATCACATTGCTAAAACGACGGAACTAAACCCTTATTGGCCCGACCCCGTGTATTTCAACACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGtt                                                                 <  1:1533072/149‑1 (MQ=255)
                                                                                       aaaTATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTg                                                                >  2:1111268/1‑150 (MQ=255)
                                                                                        aaTATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGa                                                               >  2:5456356/1‑150 (MQ=255)
                                                                                         atatCTCCATGCGAAACCGACGGAACGTAACCCTGAGAGGCCCGACTCCGCGTAGATTAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGaa                                                              <  1:6194889/150‑1 (MQ=255)
                                                                                              tcCATGCTAAAACGAAGGAACGTAACCCTGTGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAacac                                                         <  2:9005110/150‑1 (MQ=255)
                                                                                              tcCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAacac                                                         >  2:11313813/1‑150 (MQ=255)
                                                                                                   gctaaaaCTAAGTAACTTAACCCTGAGATGCCCTACTACGTTTATTTGAACACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCg                                                    <  2:1625851/147‑1 (MQ=255)
                                                                                                    ctaaaaCCACGGAATGAAACCCTGAGCGGCCCGACTCCGTGGAGATTAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCgg                                                   <  2:4158320/148‑1 (MQ=255)
                                                                                                     gAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCggg                                                  >  1:8350458/1‑150 (MQ=255)
                                                                                                     gAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCggg                                                  >  1:7271484/1‑150 (MQ=255)
                                                                                                     gAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCACGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCggg                                                  >  1:4338041/1‑150 (MQ=255)
                                                                                                      aaaaCGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCgggg                                                 >  1:3935793/1‑150 (MQ=255)
                                                                                                        aaCGACGGAAGTTAACCCTTATTTGCCCGAATCCGCGGATTTGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGaa                                               <  2:2016958/150‑1 (MQ=255)
                                                                                                           gAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAAt                                            >  2:1539425/1‑150 (MQ=255)
                                                                                                            aaGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATc                                           >  2:11505234/1‑150 (MQ=255)
                                                                                                            aaGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATc                                           >  1:7066935/1‑150 (MQ=255)
                                                                                                              tgAACGTACCCCTGATCTCCCCGACTACGTGGATTTTAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGa                                         <  1:12163457/149‑1 (MQ=255)
                                                                                                                 aCGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAAt                                      >  1:2189473/1‑150 (MQ=255)
                                                                                                                   gTAACCGTGATCTGCCCGACTACGTGGTTTTGAACACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAAttt                                    <  2:14581566/150‑1 (MQ=255)
                                                                                                                      ccccTGAGATGCCCGACTACGAGGAGATTAACACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTAtt                                 <  1:9441561/149‑1 (MQ=255)
                                                                                                                       cccTTAGATGCCCGACTACGTGGATATTAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTc                                <  2:2505552/150‑1 (MQ=255)
                                                                                                                        ccTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCt                               >  1:5095799/1‑150 (MQ=255)
                                                                                                                           gatttGCCCGACTAGTTGGATATTAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGaa                            <  1:12988709/146‑1 (MQ=255)
                                                                                                                            agaTGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAAc                           >  1:13246455/1‑150 (MQ=255)
                                                                                                                             gaTGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGaaag                          >  2:9540850/1‑148 (MQ=255)
                                                                                                                                 cccGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTg                      >  1:10598605/1‑150 (MQ=255)
                                                                                                                                   cGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTAATTCTGAACGACAGGc                    >  1:4800/1‑150 (MQ=255)
                                                                                                                                   agACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACTTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGc                    >  2:12446782/2‑150 (MQ=255)
                                                                                                                                    gACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCg                   >  2:6244269/1‑150 (MQ=255)
                                                                                                                                      cTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGaa                 >  1:9556692/1‑150 (MQ=255)
                                                                                                                                      cTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGaa                 <  2:8500906/150‑1 (MQ=255)
                                                                                                                                           tggatATTAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCTAAATTTATTCTGAACGACTGGCGAAGATTg            <  1:116387/149‑1 (MQ=255)
                                                                                                                                           aggagATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTg            >  2:4498001/1‑150 (MQ=255)
                                                                                                                                            ggagATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGc           >  1:14621552/1‑150 (MQ=255)
                                                                                                                                             catattaaaaCGACCGGTAAATTTAATAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTCAAGATCCACAGGCAAACCCGTTTAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCa          <  1:11447916/144‑1 (MQ=255)
                                                                                                                                               gaTGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCAGa        >  1:3651026/1‑150 (MQ=255)
                                                                                                                                                  taaaaCGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCAGATAc     <  1:13992402/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TACTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCAGATACCTG  >  NC_000913/1662221‑1662519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: