Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,234 C→A 17.1% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2340CA17.1% 1449.1 / 79.2 675intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (252/266);  new base A (83/54);  total (355/320)
Fisher's exact test for biased strand distribution p-value = 1.61e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 693 total aligned reads displayed.

GTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177085‑2177383
                                                                                                                                                     |                                                                                                                                                     
gTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTGTTCGATTTc                                                                                                                                                       >  2:6294970/1‑150 (MQ=255)
 ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                      <  2:14466829/150‑1 (MQ=255)
 ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                      >  2:5966631/1‑150 (MQ=255)
     cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTTCGAGTTCcaaaa                                                                                                                                                  >  2:13035086/1‑149 (MQ=255)
      aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAata                                                                                                                                                 >  1:6491640/1‑150 (MQ=255)
      aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCaaaaaa                                                                                                                                                 >  2:3041910/1‑148 (MQ=255)
       gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAatat                                                                                                                                                >  2:11547439/1‑150 (MQ=255)
       gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAatat                                                                                                                                                >  2:12230562/1‑150 (MQ=255)
        cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                               >  2:13473421/1‑150 (MQ=255)
        cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                               >  2:15120543/1‑150 (MQ=255)
          gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTCCAAAATATAAt                                                                                                                                             >  1:3539604/1‑150 (MQ=255)
           tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATg                                                                                                                                            <  2:14605763/150‑1 (MQ=255)
                accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatt                                                                                                                                       <  1:5335857/150‑1 (MQ=255)
                 ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATaattaaatta                                                                                                                                      >  2:1866712/1‑150 (MQ=255)
                  caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATTAAattat                                                                                                                                     >  2:10589469/1‑150 (MQ=255)
                  caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                     <  1:100041/150‑1 (MQ=255)
                  caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATGAAattat                                                                                                                                     >  1:13842218/1‑150 (MQ=255)
                   accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAATAATGAAaatatt                                                                                                                                    >  2:7930407/1‑150 (MQ=255)
                   accacTACCATCGTTACGAGATTGAGAATATTGAATGCCGGAACCGCATAACCCCCGCGCTGTGCGTTGTACAGCATCTGCATTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                    <  2:4784231/150‑1 (MQ=255)
                    ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                   <  2:12456831/150‑1 (MQ=255)
                       cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtt                                                                                                                                <  2:8996478/150‑1 (MQ=255)
                        ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGttt                                                                                                                               >  2:9640371/1‑150 (MQ=255)
                        ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                               >  2:5262377/1‑150 (MQ=255)
                        ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATGAAAATATtttttt                                                                                                                               >  2:3383738/1‑150 (MQ=255)
                             tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGAATTAAAAATATAATGAAAAAAATTGTTTTTaaa                                                                                                                          >  2:5727335/1‑150 (MQ=255)
                               gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAata                                                                                                                        >  1:2112890/1‑150 (MQ=255)
                                  tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                     <  2:7518610/150‑1 (MQ=255)
                                    gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCgat                                                                                                                   >  2:14819998/1‑149 (MQ=255)
                                    gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAATTATTTTTTTTTATATATCgag                                                                                                                   >  1:13037562/1‑150 (MQ=255)
                                            gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                           <  2:6479866/150‑1 (MQ=255)
                                            gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                           <  1:2022375/150‑1 (MQ=255)
                                            gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAAATAAATTATTTTTTTTTAAATATAGAGAttaagaa                                                                                                           >  1:9291243/1‑144 (MQ=255)
                                            gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATTAAAATATTTTTTTTTAAAAATCGAGATAACGAt                                                                                                           >  1:14070792/1‑150 (MQ=255)
                                            gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTCAAAATATAATGAAAATATTTTTTTTTAAATATAGAGATAAAGAt                                                                                                           >  1:3852939/1‑150 (MQ=255)
                                             catatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                          <  2:6041887/149‑1 (MQ=255)
                                               tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTAAATTATTTTTTTTTTAATATAGAGATAACGATcac                                                                                                        >  1:14473945/1‑150 (MQ=255)
                                                 ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAAAATAATGAAAATATTTGTTTTTTAATATAGAGATAAACATAACaa                                                                                                      >  1:11260059/1‑150 (MQ=255)
                                                 ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTACAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAAATATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                      >  2:12607544/1‑150 (MQ=255)
                                                    aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAATATAATGAAAATAATTTTTTTTAAAAATAGAGATAAAGATAACaaaaa                                                                                                   >  2:6729334/1‑150 (MQ=255)
                                                    aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                   <  1:3210954/150‑1 (MQ=255)
                                                    aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                   <  1:8943513/150‑1 (MQ=255)
                                                    aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                   >  2:13925617/1‑150 (MQ=255)
                                                     aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                                  <  1:597853/150‑1 (MQ=255)
                                                        ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGGTTTCGATTTGAAAAAATAATTAAAATATTTTTTTTTTAATATAGAGATAAAGATACCAAAAAAGAc                                                                                               >  1:1354269/1‑150 (MQ=255)
                                                           gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAAAAAAGAAAATATTTTTTTTTTAATATAGATGAAAAGATCACAAAAAACACaaa                                                                                            >  1:6974003/1‑149 (MQ=255)
                                                                gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATTACGGTCACAAAAAAGAAAATATGaa                                                                                       >  1:8245283/1‑150 (MQ=255)
                                                                   tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAAATAAAATATTTTTTTTTAAATATAGAGATAAAGATCAAAAAAAAAACAATAAGaaaaa                                                                                    >  1:15238201/1‑149 (MQ=255)
                                                                     aCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAatta                                                                                  <  2:8221640/150‑1 (MQ=255)
                                                                       cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt                                                                                <  2:4592125/150‑1 (MQ=255)
                                                                       cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt                                                                                <  2:3527813/150‑1 (MQ=255)
                                                                        gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAAAGAAATTATTTTTTTTTAAAAATCGAGATAACGATAACAAAAACGAAAAAATTAAAATTATTc                                                                               >  1:2316965/1‑150 (MQ=255)
                                                                        gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAATTATCACAAAAACGACAATATTAAAATTATTc                                                                               >  2:15509713/1‑150 (MQ=255)
                                                                        gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAAAGTAAATATTTTTTTTTAAATATCGAGATAACGAACACAAAAAAGACAATATTAAAATTAATc                                                                               >  2:238931/1‑150 (MQ=255)
                                                                          cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAAAGAAATTATTTGTTTTTAAATATCGAGATAAAGATCAAAAAAAACAAAAAATGAAAAATATTCga                                                                             >  1:8169333/1‑150 (MQ=255)
                                                                           gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgag                                                                            <  2:12080435/150‑1 (MQ=255)
                                                                           gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTCAAAATATAAAGAAAATATTTGTTTTTAAAAATAGATAAAACGAACACAAAAACGACAAAATTGAAATTATTagag                                                                            >  2:5499713/1‑150 (MQ=255)
                                                                             gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgagga                                                                          <  2:6939449/150‑1 (MQ=255)
                                                                             gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgaggc                                                                          >  2:11363015/1‑149 (MQ=255)
                                                                               tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGt                                                                        <  2:2636589/150‑1 (MQ=255)
                                                                               tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAAGTATTCGAGGAGt                                                                        >  2:7697318/1‑150 (MQ=255)
                                                                                         tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                              <  2:4376962/150‑1 (MQ=255)
                                                                                         tCAGCATCTGCTTTGTCGATACCACGTAAATTTTCATATCCTGTCTTTTGTTTTCTATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                              <  1:8683385/150‑1 (MQ=255)
                                                                                             cATCTGCTTTGTCGATACCACGTACATTTTCATAGCCGGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                          <  2:8669000/150‑1 (MQ=255)
                                                                                              aTCTGGTTTGTCCATACACCGTAAATTTTAATATCCTGTGGTTTGTTTTCGATTAAAAAATATAATTAAATTATTTTTTTTTAAATTTCGATATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  1:14823728/150‑1 (MQ=255)
                                                                                               tCTGCTTGGTCGTTACCACGTAATTTTTCTTATCCTGTCTTTTGTTTTCGATTAAAAAATATAATGAAATTTTTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACg                                                        <  2:11764069/150‑1 (MQ=255)
                                                                                                 tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGc                                                      <  2:2139402/150‑1 (MQ=255)
                                                                                                   cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCt                                                    >  2:10092040/1‑150 (MQ=255)
                                                                                                     ttGTCTATACCACTTACTTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTcc                                                  <  2:9383863/150‑1 (MQ=255)
                                                                                                      tttCGATACAACGTACATTTTAATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTccc                                                 <  2:13655820/148‑1 (MQ=255)
                                                                                                           aTACCACTTAAATTTTCTTAGCCTGTGTTTTTTTTTGTTTTTAAAAATATATTTAATTTTTTTTTTTTTAATTATCGTTATAAGTATAACAAAAACGACAATATGAAAATTATTCTAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAg                                            <  1:10735773/150‑1 (MQ=255)
                                                                                                            tACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAAAATGAAATTATTTGTTTTTAAAAATAGAGATAACGATCACAAAAACGACAAAAAGAAAATTATTAGAGGAGTGTAAGGCCAAAAAAAGGCCACCCCCTAgg                                           >  2:10468323/1‑150 (MQ=255)
                                                                                                               cACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGGGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                        >  2:10287162/1‑150 (MQ=255)
                                                                                                                aCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                       >  2:12777952/1‑150 (MQ=255)
                                                                                                                 cGTACATTTTCATACCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                      <  2:4301866/150‑1 (MQ=255)
                                                                                                                     ctttttCATATCCTGTCTTTTGTTTTCTATTTCAAAATATAATTAAATTTTTTTTTTTAAAATATCGAGAAAACTAACACAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                  <  1:2591337/148‑1 (MQ=255)
                                                                                                                     cATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                  <  2:2786680/150‑1 (MQ=255)
                                                                                                                      tttttCTTAGCCTGTCGTTTGTTTGCTTTTTAAAAATATCATTAATTTTTTTTTTTTTATATCGCGCTATACCGATCACAAACCCGACAATATGAAAATTTTTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                                 <  1:11793663/149‑1 (MQ=255)
                                                                                                                      aTTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACAATATGAAAATTATTAGAGGCGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGAAGCCGTAg                                 >  2:3180350/1‑150 (MQ=255)
                                                                                                                         ttCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaa                              <  2:14104468/150‑1 (MQ=255)
                                                                                                                            atatCCTGTCGTGTGTTTTAGATTTCAAAATAAAAATAAAATATTTGTTTTTAAATATAGAGATAAAGAAAACAAAAAACAAAAAAATAAAAATATTAGAGGAGTGAAAGGCAAAAAAAACTCCACCCCATAGGGCAGCCCGAGCCAAGt                           >  2:9211802/1‑150 (MQ=255)
                                                                                                                                ccTTTCTTTTGTTTTCTTTTTCAAAATATATTGAAATTTTTTTTTTTTAAATATCTATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                       <  1:5174877/150‑1 (MQ=255)
                                                                                                                                ccTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTTTTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                       <  2:492098/150‑1 (MQ=255)
                                                                                                                                    tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGTTAACGATCACAAAATCGACAATATGAAAATTATTCGTGGTGTGAAAGGCTATAAATCGGCCTCCCGATAGGGAAGCCGTAGCAATGTGCGCgtgt                   >  2:8461637/1‑150 (MQ=255)
                                                                                                                                     cttttgtttTCTATTTCAAAATATAATTAAATTTTTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                  <  1:6224396/148‑1 (MQ=255)
                                                                                                                                      tttttttttCTATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                 <  1:9865025/149‑1 (MQ=255)
                                                                                                                                      gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                 <  2:3879137/150‑1 (MQ=255)
                                                                                                                                         tgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa              <  2:7250060/150‑1 (MQ=255)
                                                                                                                                          gtttTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCTAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             <  1:4760289/150‑1 (MQ=255)
                                                                                                                                           ttttCGATTTCAAAATATAATGAATTTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg            <  2:4032375/150‑1 (MQ=255)
                                                                                                                                           ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCACAGAGCGCGTGTTTTTATg            >  1:774/1‑150 (MQ=255)
                                                                                                                                           ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg            >  1:445551/1‑150 (MQ=255)
                                                                                                                                            tttCTATTTCAAAATATAATGAAATTTTTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc           <  1:7359972/150‑1 (MQ=255)
                                                                                                                                            tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAg                                            <  2:11071757/117‑1 (MQ=255)
                                                                                                                                            tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc           >  2:1197681/1‑150 (MQ=255)
                                                                                                                                              tctaTTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg         <  1:101815/147‑1 (MQ=255)
                                                                                                                                                  tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg     >  1:1642628/1‑150 (MQ=255)
                                                                                                                                                   ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc    >  1:5593548/1‑150 (MQ=255)
                                                                                                                                                    tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg   <  1:12539640/150‑1 (MQ=255)
                                                                                                                                                    tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg   >  2:15342639/1‑150 (MQ=255)
                                                                                                                                                    tCAAAATATAATGAAATTATTTGTTTTAAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg   <  2:14357144/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177085‑2177383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: