Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,270 G→T 5.0% intergenic (‑66/+242) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2700GT 5.0% 2070.7 / 9.8 721intergenic (‑66/+242)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (319/330);  new base T (8/62);  total (327/394)
Fisher's exact test for biased strand distribution p-value = 2.63e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 751 total aligned reads displayed.

GAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATA  >  NC_000913/2177121‑2177419
                                                                                                                                                     |                                                                                                                                                     
gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATagag                                                                                                                                                       >  2:8307503/1‑150 (MQ=255)
     tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAc                                                                                                                                                  <  1:6311056/150‑1 (MQ=255)
      gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACg                                                                                                                                                 >  2:13973083/1‑150 (MQ=255)
        gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGAt                                                                                                                                               >  1:13077743/1‑150 (MQ=255)
          atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                                                             <  1:13598828/150‑1 (MQ=255)
              tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTAAAATATTTTTTTTTAAATATAGAGTTAACGATCACaaa                                                                                                                                         >  1:7380218/1‑150 (MQ=255)
                aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACaaaaa                                                                                                                                       >  2:11868581/1‑150 (MQ=255)
                aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGATAACaaaaa                                                                                                                                       >  1:4584620/1‑150 (MQ=255)
                 aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                                                                      <  2:6908831/150‑1 (MQ=255)
                    ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAc                                                                                                                                   <  2:14533013/150‑1 (MQ=255)
                     cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACa                                                                                                                                  >  1:7784984/1‑150 (MQ=255)
                     cGGAACCGCATAACCGCCGCGCGGTGCGTTGTTCAGCATCTGCTGTGGCGATACCACGTACATTTTCATATCCTGTCGTTTGGTTTCGATTTCAAAATATAATGAAAATATTTGGTGTTAAATAACGAGAGAACGATCACAAAAACGACa                                                                                                                                  >  2:15508630/1‑150 (MQ=255)
                      ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTTTTTTTTAATATAGAGATTACGATAACAAAAACGaaaa                                                                                                                                 >  1:10194244/1‑150 (MQ=255)
                         aCCGCATACCCGGCGCGCTGTGCGTTGTTCAGCATGTGCTTTGTCGATACCACGTACTTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGTTAACGATCACAAAAACGACAatat                                                                                                                              <  2:11065434/150‑1 (MQ=255)
                          ccGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATAACAAAAACGACAatatt                                                                                                                             >  2:15358563/1‑149 (MQ=255)
                            gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTAAAAATATAATTAAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAAAGACAATATGaa                                                                                                                           >  1:1229907/1‑150 (MQ=255)
                             cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAAAGACAATATGaaa                                                                                                                          >  2:9094663/1‑150 (MQ=255)
                             cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATGAAATTATTTGTTTTTAAATATAGAGGTAACGATCACAAAAAAGACAAAAAGaaa                                                                                                                          >  1:879396/1‑150 (MQ=255)
                             cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAAAGAAATTATTTGTTTTTAAATATAGAGATAAAGATAACAAAAACGACAAAATTaaa                                                                                                                          >  1:9451562/1‑150 (MQ=255)
                             cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTAATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGaaa                                                                                                                          <  2:5398866/150‑1 (MQ=255)
                              aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAATATAATGAAAATATTTTTTTTTAAATATAGAGATAAACATCACAAAAACGAAAAAATGaaaa                                                                                                                         >  2:4170409/1‑150 (MQ=255)
                                aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTGAAAATATTAAGTAATTATTTTTTTTTTAATATAGAGATAAAGATAAAAAAAAAGACAATAAGAAAatt                                                                                                                       >  1:7145569/1‑150 (MQ=255)
                                   cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATAATTAAAATATTTTTTTTTTAATTTAGGGATAACGAAAACAAAAAACAAACAAAGAAAattatt                                                                                                                    >  1:213200/1‑150 (MQ=255)
                                    gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAAAGTAAATATTTTTTTTTAAATATCGAGATAACGAACACAAAAAAGACAATATTAAAATTAATc                                                                                                                   >  2:238931/1‑150 (MQ=255)
                                         gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgagga                                                                                                              <  2:6183544/150‑1 (MQ=255)
                                         gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgaggc                                                                                                              >  2:11363015/1‑149 (MQ=255)
                                           tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGt                                                                                                            <  2:2636589/150‑1 (MQ=255)
                                            gtgCGTTGTTCAGCATCCGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTg                                                                                                           <  2:5564066/150‑1 (MQ=255)
                                                 ttgttCAGCATCTGCTTTGTCGTTACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGATTTGAAAATATAATTAAATTATTTGTTTTTTAATATGGAGTTAAAGTTCTCAAAAAAGTCAATATGTAATTTTTTGGTGGTGTGTAAgg                                                                                                      >  2:8633878/1‑150 (MQ=255)
                                                   gttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCa                                                                                                    <  2:716563/150‑1 (MQ=255)
                                                    ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAAAATAAAGAAATTATTTGTTTTTAAATATCGCGCGAACCAGCACACAAACCACAAAATGGAAATTATTCGAGGCGGGAAAGGccc                                                                                                   >  2:95737/1‑148 (MQ=255)
                                                    ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAATAAAAATAAAATATTTTTTTTTATATATATAGATAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAATTAGATGGGTGAAAGTCaa                                                                                                   >  1:14318682/1‑150 (MQ=255)
                                                     tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAATATAAAGAAAATATTTGTTTTTAAATATAGAGATAACGATAACAAAAAAGACAAAATTAAAATTATTAGAGGAGTGAAAGGCaaa                                                                                                  >  2:4576661/1‑150 (MQ=255)
                                                     tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                                                                  >  2:3527682/1‑150 (MQ=255)
                                                        gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAAAATAATTAAATTATTTTTTTTTTAATATAGAGATAAAGATAAAAAAAAAGAAAATATTTAAAATATTTGTGGAGTTAAATGGaaaaaa                                                                                               >  1:8205993/1‑150 (MQ=255)
                                                          aTCTGCTTTGTCGATACCACGTACTTTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATATTTAAATTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                                             <  1:8633075/150‑1 (MQ=255)
                                                          aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTTTTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                                             <  2:8846706/150‑1 (MQ=255)
                                                          aTCTGATTTGTCGAAACCACTTAATTTTTCATATCCTGTCTTTTTTTTTCTTTTTAAAAATATAATTAATTTATTTTTTTTTAAATATCGATATAACTATAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                                             <  1:1412207/150‑1 (MQ=255)
                                                                 ttttCGATACCACTTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTcc                                                                                      <  2:3049990/147‑1 (MQ=255)
                                                                 ttGTCTATACCACTTACTTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTcc                                                                                      <  2:9383863/150‑1 (MQ=255)
                                                                  tttCGATACAACGTACATTTTAATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTccc                                                                                     <  2:13655820/148‑1 (MQ=255)
                                                                        tACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAATTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCgg                                                                               <  2:2964316/150‑1 (MQ=255)
                                                                           cACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGGGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                                                            >  2:10287162/1‑150 (MQ=255)
                                                                            aCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTTTTTTTTTTTAAATATAGAGATAAAGATCAACAAAACGAGAAAATTGAAATTATACGGGGAGGGAAAGGGAATAAAACGGCCTCCGGATGGGGGAg                                                                           >  2:5810554/1‑150 (MQ=255)
                                                                            aCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                                                           >  2:12777438/1‑150 (MQ=255)
                                                                             cGTACATTTTCATCTCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTAAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                                                          <  2:7341632/150‑1 (MQ=255)
                                                                             cGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                                                          <  2:11681755/150‑1 (MQ=255)
                                                                              gaaCATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                                                         <  2:10516438/148‑1 (MQ=255)
                                                                              gTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                                                         <  2:13504911/150‑1 (MQ=255)
                                                                                 cATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                                                      <  2:6566188/150‑1 (MQ=255)
                                                                                    tttCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTGGCGGGGTGTAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCa                                                                   >  2:12054473/1‑150 (MQ=255)
                                                                                     ttCATATCCTGTCGTTTTTTTTCGATTTAAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaa                                                                  <  2:1774334/150‑1 (MQ=255)
                                                                                          atCCTGTCTTTTGTTTTCTTTTAAAAAATTTAATGAATTTTTTTTTTTTTAAATATCTATAAAACGAACAAAAAAACTAAAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgc                                                             <  1:6676894/150‑1 (MQ=255)
                                                                                            ccTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTTTTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                                                           <  2:492098/150‑1 (MQ=255)
                                                                                                tCGTTTTTTTTCTATTACAAAATATAATTAATTTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                                                       <  1:9211802/150‑1 (MQ=255)
                                                                                                   ttttttttAGATTTAAAAATATAATTAAATTATTTTTTTTTAAATATCTATATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                                                    <  1:6013216/150‑1 (MQ=255)
                                                                                                      gtttTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCTAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                                                 <  2:6015876/150‑1 (MQ=255)
                                                                                                        tttctatttctaTATATTTTTATATTATTTGTTTTAAAATATCGTTATAACGTTAACAAAAACGACAATATGAAAATTATTCGAGGTTTGAATGGCAAAAAAACGGCCTACCGATAGGGAAGCCGTAGCAAAGTGCGCTTGTTTTTATGc                                               <  1:2362397/150‑1 (MQ=255)
                                                                                                        tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAg                                                                                >  1:11071757/1‑117 (MQ=255)
                                                                                                        tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTGTTTATGc                                               >  1:13933811/1‑150 (MQ=255)
                                                                                                         ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                                              >  2:3958405/1‑150 (MQ=255)
                                                                                                           cGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg                                            >  2:12548067/1‑150 (MQ=255)
                                                                                                            gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                                           <  2:3241305/150‑1 (MQ=255)
                                                                                                             aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTACCGATAGGGAAGCCGTAGAAAAGTGCGCGTGTTTTTATGCCGGAt                                          >  1:10638065/1‑150 (MQ=255)
                                                                                                                tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                       >  2:780614/1‑150 (MQ=255)
                                                                                                                tCAAAATATAATGAAATTATTTGTTTTAAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                       <  2:14357144/150‑1 (MQ=255)
                                                                                                                  aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt                                     <  1:5497862/150‑1 (MQ=255)
                                                                                                                  aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACTACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt                                     >  1:3385809/1‑150 (MQ=255)
                                                                                                                  aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt                                     >  1:1132220/1‑150 (MQ=255)
                                                                                                                  aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt                                     >  2:12260108/1‑150 (MQ=255)
                                                                                                                    aaTATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt                                   <  2:2115300/150‑1 (MQ=255)
                                                                                                                       ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                <  2:8169333/150‑1 (MQ=255)
                                                                                                                           tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa                            >  1:13001529/1‑150 (MQ=255)
                                                                                                                           tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa                            <  1:4249612/150‑1 (MQ=255)
                                                                                                                            gAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc                           >  2:1510847/1‑150 (MQ=255)
                                                                                                                            gAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc                           >  1:11656203/1‑150 (MQ=255)
                                                                                                                             aaaTTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACg                          <  1:9707277/150‑1 (MQ=255)
                                                                                                                             aaaTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACg                          <  2:10856561/150‑1 (MQ=255)
                                                                                                                             aaaTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACg                          >  1:2660981/1‑150 (MQ=255)
                                                                                                                              aaTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGt                         >  1:1539903/1‑150 (MQ=255)
                                                                                                                              aaTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGt                         >  1:4917087/1‑150 (MQ=255)
                                                                                                                                   tttGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAg                    <  1:10001518/150‑1 (MQ=255)
                                                                                                                                     tGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTg                  <  1:9017094/150‑1 (MQ=255)
                                                                                                                                      gTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                       >  2:4373643/1‑128 (MQ=255)
                                                                                                                                       tttttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCt                >  1:3710530/1‑150 (MQ=255)
                                                                                                                                       tttttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCt                <  1:1866712/150‑1 (MQ=255)
                                                                                                                                       tttttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCt                <  2:14132958/150‑1 (MQ=255)
                                                                                                                                         tttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATCCGTGGCAGTGCTAt              <  2:7744496/150‑1 (MQ=255)
                                                                                                                                         tttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTAt              <  1:12560533/150‑1 (MQ=255)
                                                                                                                                         tttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTAt              <  2:14855821/150‑1 (MQ=255)
                                                                                                                                          ttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATg             <  1:3175699/150‑1 (MQ=255)
                                                                                                                                          ttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATg             >  1:5233239/1‑150 (MQ=255)
                                                                                                                                          ttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATg             >  2:7608385/1‑150 (MQ=255)
                                                                                                                                            aaaTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTa           <  2:13093144/150‑1 (MQ=255)
                                                                                                                                            aaaTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTa           <  1:8426867/150‑1 (MQ=255)
                                                                                                                                            aaaTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTa           <  1:15038031/150‑1 (MQ=255)
                                                                                                                                             aaTATCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAg          <  2:10039926/150‑1 (MQ=255)
                                                                                                                                                atCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCa       >  1:14807658/1‑150 (MQ=255)
                                                                                                                                                 tCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCAt      >  2:4732595/1‑150 (MQ=255)
                                                                                                                                                    agaTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGAt   >  2:12969314/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATA  >  NC_000913/2177121‑2177419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: