Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,185,435 G→T 7.3% intergenic (‑134/+89) thiM ← / ← rcnR hydoxyethylthiazole kinase/transcriptional repressor of rcnA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,185,4350GT 7.3% 2526.3 / 28.1 800intergenic (‑134/+89)thiM/rcnRhydoxyethylthiazole kinase/transcriptional repressor of rcnA
Reads supporting (aligned to +/- strand):  ref base G (255/458);  new base T (85/2);  total (340/460)
Fisher's exact test for biased strand distribution p-value = 4.22e-32
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 807 total aligned reads displayed.

GCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGC  >  NC_000913/2185286‑2185584
                                                                                                                                                     |                                                                                                                                                     
 cagGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGc                                                                                                                                                      <  1:9534121/150‑1 (MQ=255)
   ggTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCga                                                                                                                                                    <  2:8184870/150‑1 (MQ=255)
    gTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCgat                                                                                                                                                   >  2:6169445/1‑150 (MQ=255)
          ttGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATaaa                                                                                                                                             >  1:3551164/1‑150 (MQ=255)
          ttGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATaaa                                                                                                                                             >  1:11568342/1‑150 (MQ=255)
            gCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATc                                                                                                                                           <  2:10747607/150‑1 (MQ=255)
             cATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCga                                                                                                                                                    >  1:8440232/1‑140 (MQ=255)
             cATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCg                                                                                                                                          >  1:13319505/1‑150 (MQ=255)
                 gTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCaga                                                                                                                                      <  2:11718064/150‑1 (MQ=255)
                  tttGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAAAAGCagag                                                                                                                                     >  2:4835537/1‑150 (MQ=255)
                     gCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGGGGGTGGGCTGATAAAAAGCAgagggg                                                                                                                                  >  1:2713022/1‑148 (MQ=255)
                       tCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCa                                                                                                                                        >  2:12750235/1‑142 (MQ=255)
                       tCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAggagga                                                                                                                                <  2:6539588/150‑1 (MQ=255)
                       tCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAggagga                                                                                                                                <  1:15284384/150‑1 (MQ=255)
                       tCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTTCGATGATAAATAGCAGAtgagga                                                                                                                                >  1:14011574/1‑150 (MQ=255)
                       tCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGGGGTTTCGATGAGAAATCGCAGaggaggg                                                                                                                                >  2:7002260/1‑149 (MQ=255)
                         cTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATg                                                                                                                              <  2:774633/150‑1 (MQ=255)
                         cTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATg                                                                                                                              >  1:2002719/1‑150 (MQ=255)
                         cTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGGTGCGATGTTAAATAGCAGCGGCGGCTg                                                                                                                              >  2:173958/1‑150 (MQ=255)
                          tGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAATCGCAGAGGAGGATgg                                                                                                                             >  1:5023390/1‑150 (MQ=255)
                           gCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGt                                                                                                                            <  2:10201113/150‑1 (MQ=255)
                            ccATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTa                                                                                                                           <  1:10911099/150‑1 (MQ=255)
                            ccATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAATCGCAGAGGAGGATGGTa                                                                                                                           >  2:1145751/1‑150 (MQ=255)
                             cATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTaa                                                                                                                          <  1:3949639/150‑1 (MQ=255)
                               tAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGGTGCGATGATAAATAGCAGAGGAGGATGGTAATg                                                                                                                        >  2:2013879/1‑150 (MQ=255)
                                   gTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCa                                                                                                                    <  2:14262451/150‑1 (MQ=255)
                                   gTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAAACGCAGAGGAGGATGGTAATGTCCa                                                                                                                    >  1:15398569/1‑150 (MQ=255)
                                    tGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAg                                                                                                                   <  2:12224507/150‑1 (MQ=255)
                                      aagaagCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGGTAAAAAGCAGAGGAGGATGGTAGAGTCCcgcg                                                                                                                 >  2:13493607/1‑150 (MQ=255)
                                        gaagCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGGGGGTGCGATCAAAAATCGCACAGGAGGGTGGTAATGTCCACCGCa                                                                                                               >  2:14516795/1‑150 (MQ=255)
                                         aagCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCAc                                                                                                              <  1:460748/150‑1 (MQ=255)
                                         aagCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCAc                                                                                                              >  2:1308293/1‑150 (MQ=255)
                                         aagCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGAGGATAAAACGCCGAGGAGGATGGTAAAGTCCAGCGCAc                                                                                                              >  1:11919350/1‑150 (MQ=255)
                                             aaTGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGt                                                                                                          <  1:5748464/150‑1 (MQ=255)
                                              aTGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTTGTTTGGGTGTTTAATAGGAGAGGTGGTTGGTTTTGTGGAGGGCACGCGtt                                                                                                         >  1:6036579/1‑150 (MQ=255)
                                              aTGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAAAAGCAGAGGAGGATGGTAATTTCCAGCGCAcgcggt                                                                                                         >  2:3293015/1‑148 (MQ=255)
                                              aTGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTTCGATGTTAAATAGCAGAGGAGGGTGGTAATGTCCAGCGCCCGCGtt                                                                                                         >  1:7243118/1‑150 (MQ=255)
                                                gACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGt                                                                                                       <  1:12923740/150‑1 (MQ=255)
                                                 aCCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGGTGTTTAATGGCAGAGGTGGGTGGTAATTTGCAGCGCACGCGTTGTa                                                                                                      >  2:4346490/1‑150 (MQ=255)
                                                    tggtggTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTTCGATTATAAAAAGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAAc                                                                                                   >  2:5874316/1‑150 (MQ=255)
                                                      gtggtCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGa                                                                                                 <  1:6851683/150‑1 (MQ=255)
                                                      gtggtCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGa                                                                                                 <  1:10590375/150‑1 (MQ=255)
                                                      gtggtCCGTGACGTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGa                                                                                                 <  2:12565259/150‑1 (MQ=255)
                                                          tCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTTCGATGCTAAATCGCAGCGGAGGATGGTAATGTCCAGCCCACGCGTTGTAAACGAGGaa                                                                                             >  2:7946160/1‑150 (MQ=255)
                                                            cGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGGGATGATAAATCGCAGAGGAGGAGTGTAATGTCCAGCGCACGCGTTGTAAACGAGGaaaa                                                                                           >  1:2366855/1‑150 (MQ=255)
                                                            cGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGTTAAAACGCAGAGGAGGATGGTAAAGTCCAGCGCACGCGGTGTAAACGAGGaaaa                                                                                           >  1:12217400/1‑150 (MQ=255)
                                                            cGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATAGCAGAGGAGGATGGTAATGTCCAGCGCACCCGTTGTAAAAGAGGaaaa                                                                                           >  1:8990842/1‑150 (MQ=255)
                                                              tGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAAc                                                                                         <  1:2945718/150‑1 (MQ=255)
                                                               gACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACg                                                                                        >  2:5094017/1‑150 (MQ=255)
                                                                aCTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTTCGATTATAAATAGAAGAGGAGGGTGGTAATGTCCAGCGCACCCGTTGTTAAAGAGGaaaaaacg                                                                                       >  1:1696232/1‑147 (MQ=255)
                                                                 cTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGc                                                                                      <  2:13230867/150‑1 (MQ=255)
                                                                 cTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGGGATTATAAAAAGCAGAGGAGGATGGTTATTTCCCGCGCACGCGTTGTAAACGAGGaaaaaaagc                                                                                      >  1:4806222/1‑146 (MQ=255)
                                                                        aCGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTa                                                                               <  2:15364259/150‑1 (MQ=255)
                                                                        aCGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTa                                                                               <  2:9446978/150‑1 (MQ=255)
                                                                              gCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATcg                                                                         <  2:14113290/150‑1 (MQ=255)
                                                                                    tCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTTCGATGATAAAAAGCAGAGGAGGATGGTAAAGTCCAGCGCACGCGTTTTAAAAGAGGAAAAACGGCAGTTTTAAAAAAGCGTtaa                                                                   >  2:731651/1‑150 (MQ=255)
                                                                                    tCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGACACCCCGAGTCGTGTGGTTGGGATTATAAAAAGCAGAGGAGGGTGGTGATGTCCCGCGCCCGCGTTGTTAACGAGGGAAAAAGGCAGTTTTAAAATcgcggtta                                                                   >  1:6421786/1‑146 (MQ=255)
                                                                                      cAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTTGGGTGATAAATAGCAGAGGAGGATGGTTATGTCCAGCGCACCCCGTGTAAACGAGGAAAAAACGCAGGTTTTAAAAcgcggttaaa                                                                 >  1:10350937/1‑144 (MQ=255)
                                                                                       aGATCTGGTGTTAGGGGTATTTCTCAGCCTTCCCGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGTTGATAAATCGCAGAGGTGGATGGTAATGTCCTGCGCACGCGTTGTAAACGTGGAAAAACGGCAGTTTTACAATCGCGTtaataa                                                                <  1:11290223/150‑1 (MQ=255)
                                                                                       aGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAAACGCAGAGGGGGTTTGTAAATTACAGCGCACCCCGTGTAAAAGAGGAAAAAAGGCACTTTTAAAAGCGCGTtaataa                                                                >  2:5495951/1‑150 (MQ=255)
                                                                                        gATCAGGTGATACGGTTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCTATTATAAATCGCAGTGGAGGTTGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATaaa                                                               <  1:14801753/150‑1 (MQ=255)
                                                                                             ggTTATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGTGTCGTTTGGTTGCTATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAattatt                                                          <  1:3444739/150‑1 (MQ=255)
                                                                                             ggTGATTCGGGTATTTCTCAGCCTTCACGCAGTAGGGCACCGCGAGTCGTTTGGTTGCGATGATAAATCGGAGAGGTGGATGGTAATGTCCAGCGCACGCGTTGTTAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAattatt                                                          <  2:9708691/150‑1 (MQ=255)
                                                                                             ggTGATACGGGTATTTCTCAGCCTTCACGCAGTAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAattatt                                                          <  2:10486676/150‑1 (MQ=255)
                                                                                                gATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGa                                                       <  2:4139591/150‑1 (MQ=255)
                                                                                                  tACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGTGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGTTGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGata                                                     <  1:9194801/150‑1 (MQ=255)
                                                                                                  tACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGata                                                     <  2:8792352/150‑1 (MQ=255)
                                                                                                  tACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGata                                                     <  2:2609584/150‑1 (MQ=255)
                                                                                                     ggttATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGTTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGatatat                                                  <  1:4191421/147‑1 (MQ=255)
                                                                                                     gggTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTTCGATGATAAATAGCAGAGGAGGATGGTGATTTACCGCGCACGCGAGGGAAAACAGGAAAAAAGGCCCTTTTACAAACGCGGTAATAAAATAATTTttatat                                                  >  1:15081568/1‑150 (MQ=255)
                                                                                                     gggTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATTATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTTTTAACGAGGAAAAAAGGCAGTTTTACAATCGCGTTAATAAATTATTTGatatat                                                  >  2:461440/1‑150 (MQ=255)
                                                                                                      ggTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGGGTGGTTGCGATGATAAAACGCCGAGGAGGCGGGTACAGTCCCCCCCCCCCGGTGTAAACCCGGAAAAACCGCAGGTTTACAAGCGCGGTAAAAACATAGTTGGTATATg                                                 >  2:8364363/1‑150 (MQ=255)
                                                                                                             ctcAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAg                                          <  2:6904627/150‑1 (MQ=255)
                                                                                                              tcAGCCTTCACGCAGAAGGGCACCCCGAGGCGTGTGGTTGCGAGGCTAAATCGCAGAGGCGGCGGGTCATGTCCCGCGCCCGCGTTGTAAACGACGAAAAAAGGCAGTTTTACAAGCGCGGTAATAAATTAATTGATATATGAATCCAGc                                         >  2:15262459/1‑150 (MQ=255)
                                                                                                               cAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCa                                        <  2:2837872/150‑1 (MQ=255)
                                                                                                                   cTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCtt                                    <  1:10012456/150‑1 (MQ=255)
                                                                                                                     tCACGCAGAAGGGCACCCCGAGTCGTGTGGTTTCGATTATAAAAAGCAGAGGAGGATTGTAAATTCCAGCGCAAGCGTTTTAAAAGAGGAAAAAAGGCAGTTTTAAAAAAGCGGTAATAAAATATTTTATATATTAAAACAGCAACATCa                                  >  2:5742470/1‑150 (MQ=255)
                                                                                                                     tCACGCAGAAGGGCACCCCGAGACGTTTGGTTGCGATGATAAATCGCAGAGGAGTATGGTAATGTCCAGCGCACGCGATGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCa                                  <  2:11063418/150‑1 (MQ=255)
                                                                                                                      cACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAg                                 <  1:9916168/150‑1 (MQ=255)
                                                                                                                       aCGCAGAAGGGCACCCCGAGTCGTTTGGTTTCGATTATAAATAGCAGATGAGGATGTTAATTTACAGCGCACCCGTTTTAAAAGAGGAAAAAAGGGAGTTTTTAAATCGCGTTATTAAATTATTTGATATATTAATACAGCAACttaata                                >  2:13940670/1‑146 (MQ=255)
                                                                                                                        cGCAGAAGGGCACCCCGTGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGaa                               <  1:1785653/150‑1 (MQ=255)
                                                                                                                        cGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGaa                               <  2:13650363/150‑1 (MQ=255)
                                                                                                                        cGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGaa                               <  2:13769968/150‑1 (MQ=255)
                                                                                                                        cGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGaa                               <  2:15580361/150‑1 (MQ=255)
                                                                                                                         gCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAAc                              <  1:7543604/150‑1 (MQ=255)
                                                                                                                         gCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAAc                              <  2:9151629/150‑1 (MQ=255)
                                                                                                                            gAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGAc                           <  1:7970026/150‑1 (MQ=255)
                                                                                                                             aaGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACa                          <  2:755026/150‑1 (MQ=255)
                                                                                                                              aGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGTGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACAt                         <  2:12386419/150‑1 (MQ=255)
                                                                                                                                  cACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATAGCGTTAATAAATTATTTGATATATGAATACAGCACCTTCAGAACGACAACCAg                     >  2:8673122/1‑150 (MQ=255)
                                                                                                                                       cGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGTAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGAtctt                <  1:14369394/150‑1 (MQ=255)
                                                                                                                                        gAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGAtcttc               <  2:3931064/150‑1 (MQ=255)
                                                                                                                                        gAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGAtcttc               <  2:3551164/150‑1 (MQ=255)
                                                                                                                                          gTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATcttctt             <  2:2354534/150‑1 (MQ=255)
                                                                                                                                              tttGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACg         >  2:14846790/1‑150 (MQ=255)
                                                                                                                                              tttGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACg         >  1:9708688/1‑150 (MQ=255)
                                                                                                                                              tttGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACg         >  2:120092/1‑150 (MQ=255)
                                                                                                                                                 ggTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGttt      <  1:12701464/150‑1 (MQ=255)
                                                                                                                                                  gTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGtttt     <  1:13538762/150‑1 (MQ=255)
                                                                                                                                                     gCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGc  >  1:5392984/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGC  >  NC_000913/2185286‑2185584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: