Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,185,446 T→A 8.4% intergenic (‑145/+78) thiM ← / ← rcnR hydoxyethylthiazole kinase/transcriptional repressor of rcnA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,185,4460TA 8.4% 2319.4 / 25.2 790intergenic (‑145/+78)thiM/rcnRhydoxyethylthiazole kinase/transcriptional repressor of rcnA
Reads supporting (aligned to +/- strand):  ref base T (259/435);  new base A (92/2);  total (353/437)
Fisher's exact test for biased strand distribution p-value = 4.29e-33
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 809 total aligned reads displayed.

TGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTG  >  NC_000913/2185297‑2185595
                                                                                                                                                     |                                                                                                                                                     
tGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAAt                                                                                                                                                       <  2:4068285/150‑1 (MQ=255)
 gCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATc                                                                                                                                                      >  1:15215054/1‑150 (MQ=255)
  cATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCg                                                                                                                                                     <  2:10806094/150‑1 (MQ=255)
    tAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCa                                                                                                                                                   <  1:7406778/150‑1 (MQ=255)
    tAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCa                                                                                                                                                   <  1:9026768/150‑1 (MQ=255)
    tAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCa                                                                                                                                                   <  1:11516575/150‑1 (MQ=255)
       tttGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCagag                                                                                                                                                <  2:14998123/150‑1 (MQ=255)
       tttGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCagag                                                                                                                                                >  2:10301296/1‑150 (MQ=255)
       tttGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAATAGCagag                                                                                                                                                >  1:3401390/1‑150 (MQ=255)
         tGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAgagga                                                                                                                                              <  1:8049662/150‑1 (MQ=255)
         tGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAgagga                                                                                                                                              <  1:4661054/150‑1 (MQ=255)
            tCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAggagga                                                                                                                                           <  1:3537478/150‑1 (MQ=255)
            tCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTTCGATGATAAATAGCAGAggagga                                                                                                                                           >  1:11977396/1‑150 (MQ=255)
            tCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAATCGCAGAggagga                                                                                                                                           >  1:10832484/1‑150 (MQ=255)
               tGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGAAgg                                                                                                                                        >  2:10099648/1‑150 (MQ=255)
                 ccATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTTCGATGATAAATAGCAGAGGAGGATGGTa                                                                                                                                      >  1:10476936/1‑150 (MQ=255)
                 ccATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAATAGCAGAGGAGGATGGTa                                                                                                                                      >  1:5220146/1‑150 (MQ=255)
                    tAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATg                                                                                                                                   <  2:2935889/150‑1 (MQ=255)
                    tAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATg                                                                                                                                   >  1:15580361/1‑150 (MQ=255)
                    tAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGGTTCGATGATAAATCGCCGAGGCGGGTGGTAATg                                                                                                                                   >  2:1391655/1‑150 (MQ=255)
                      aCGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTc                                                                                                                                 <  1:15024889/150‑1 (MQ=255)
                         tGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAg                                                                                                                              <  1:9277262/150‑1 (MQ=255)
                          gaagaaGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAgc                                                                                                                             <  2:2624215/150‑1 (MQ=255)
                           aagaagCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAgcg                                                                                                                            <  2:7608037/150‑1 (MQ=255)
                            agaagCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGACGGGCACCCCGAGTCGTGTGGGTGCGCTGATAAATCGCCGAGGAGGATGGTAATGTCCCgccc                                                                                                                           >  1:3896252/1‑148 (MQ=255)
                            agaagCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAgcgc                                                                                                                           <  2:12403390/150‑1 (MQ=255)
                              aagCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAATAGCAGAGGAGGATGGTAATGTCCCGCGCAc                                                                                                                         >  1:1127751/1‑150 (MQ=255)
                                  aaTGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGt                                                                                                                     <  2:10521613/150‑1 (MQ=255)
                                  aaTGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATTATAAATAGCAGAGGAGGATGGTAATGTCCAGCGCAcgcgg                                                                                                                     >  1:247597/1‑149 (MQ=255)
                                  aaTGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAAAAGCCCAGGAGGCTGGTAATGGcccgcgcccgcgg                                                                                                                     >  1:14799723/1‑149 (MQ=255)
                                     gACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGt                                                                                                                  <  1:12923740/150‑1 (MQ=255)
                                        cTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTaaa                                                                                                               <  2:11762547/150‑1 (MQ=255)
                                        cTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGGGGTGATAAATAGCAGAGGAGGATGGTAATGTCCAGCGCACCCGTTGTaaa                                                                                                               >  2:212616/1‑150 (MQ=255)
                                        cTGGTGGTCCGTGACTTCACTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGGTTAGATGATAAAGCGCAGAGGAGGATGGTGAAGTCCCCCCCACCAGTTGTaaa                                                                                                               >  2:14407336/1‑150 (MQ=255)
                                         tggtggTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTTCGATTATAAAAAGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAAc                                                                                                              >  2:5874316/1‑150 (MQ=255)
                                           gtggtCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGa                                                                                                            <  1:40586/150‑1 (MQ=255)
                                           gtggtCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGa                                                                                                            <  1:7002260/150‑1 (MQ=255)
                                           gtggtCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGa                                                                                                            <  1:10330909/150‑1 (MQ=255)
                                           gtggtCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGa                                                                                                            <  2:6367970/150‑1 (MQ=255)
                                           gtggtCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGa                                                                                                            <  1:1858552/150‑1 (MQ=255)
                                            tggtCCGTGACTTCCCTACGCTGACATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTTCGCTGATAAATAGCAGAGGAGGATGGTAATGTCCAGCGCACGCGGTTTAAACGAg                                                                                                           >  1:7647179/1‑150 (MQ=255)
                                               tCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGCTGATAAAACGCAGAGGAGGATTGTAATGTCCAGCGCACGCGTTGTAAACGAGGaa                                                                                                        >  2:7946307/1‑150 (MQ=255)
                                                 cGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGaaaa                                                                                                      <  1:1769590/150‑1 (MQ=255)
                                                 cGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTTGGATGATAAAACGCAGCGGAGGATGGTAATGTCCAGCGCCCCCGGTGTAAACGAGGaaaa                                                                                                      >  1:6806902/1‑150 (MQ=255)
                                                 cGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGAAGATAAATCGCAGAGGAGGATGGTAAAGTCCCGCGCACCCGTTGTAAACGAGGaaaa                                                                                                      >  2:14095154/1‑150 (MQ=255)
                                                  gTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGaaaaa                                                                                                     <  1:14010542/150‑1 (MQ=255)
                                                   tGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAAc                                                                                                    <  1:2945718/150‑1 (MQ=255)
                                                   tGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAAc                                                                                                    <  1:15344092/150‑1 (MQ=255)
                                                     aCTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAGGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACgg                                                                                                  <  2:6747745/150‑1 (MQ=255)
                                                     aCTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTaa                                                                                                                <  1:1273848/136‑1 (MQ=255)
                                                     aCTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAATCGCAGCGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACgg                                                                                                  >  2:13757063/1‑150 (MQ=255)
                                                          ccTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGGTGATAAAACGCCGAGGAGGATGGTAATGTCCCGCGCACGCGGTGTAAACGAGGAAAAACGGCAGtt                                                                                             >  1:4940884/1‑150 (MQ=255)
                                                           cTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAATGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGttt                                                                                            <  2:8766584/150‑1 (MQ=255)
                                                                 tGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAATCGCAGAGGGGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAAt                                                                                      >  2:1156979/1‑150 (MQ=255)
                                                                   gCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGTGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATcg                                                                                    <  2:12893072/150‑1 (MQ=255)
                                                                        aTCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAAACGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTta                                                                               >  2:9079280/1‑150 (MQ=255)
                                                                         tCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAAACGCAGAGGAGGATGGTAATTTCCAGCGCACGCGTTGTAAAAGAGGAAAAACGGCAGTTTTACAAGAGCGTtaa                                                                              >  2:3318003/1‑150 (MQ=255)
                                                                          ccATATCAGGTGATACGGGTATTTCTCCGCCTTCACGCAGAAGGGCACCCCGAGTCTTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTtaat                                                                             <  1:9637890/150‑1 (MQ=255)
                                                                          ccAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTTCGATGATAAATAGCAGAGGAGGGTTGTAATGTACAGCGCAAGCGGTGTAAAACAGGAAAAAAGGCAGTTTTACAAACGCGTtaat                                                                             >  2:1422520/1‑150 (MQ=255)
                                                                            aGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAAACGCAGAGGGGGTTTGTAAATTACAGCGCACCCCGTGTAAAAGAGGAAAAAAGGCACTTTTAAAAGCGCGTtaataa                                                                           >  2:5495951/1‑150 (MQ=255)
                                                                             gATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATaaa                                                                          <  2:13319505/150‑1 (MQ=255)
                                                                                   gTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTAttt                                                                    >  2:2369275/1‑150 (MQ=255)
                                                                                     gATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGa                                                                  <  2:13558236/150‑1 (MQ=255)
                                                                                     gATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGa                                                                  <  2:6959311/150‑1 (MQ=255)
                                                                                       tACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGata                                                                <  2:1651894/150‑1 (MQ=255)
                                                                                          gggTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGatatat                                                             <  2:11188720/150‑1 (MQ=255)
                                                                                          gggTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGatatat                                                             <  2:15315295/150‑1 (MQ=255)
                                                                                          gggTATGTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGatatat                                                             <  2:9686788/150‑1 (MQ=255)
                                                                                           ggTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAAACGCAGAGGGGGTTGGTTAAGTGCAGCGCACGCGGTTTAAAAGATGGAAAACGGCAGTTTTACAAACGCGGTAATAAAATAATTGTTATATg                                                            >  2:4329913/1‑150 (MQ=255)
                                                                                             tATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTTCGATGATAAATAGCAGAGGAGGATGGTAATTTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTAAAAGCGCGTTAATAAATTATTTGATATATTaa                                                          >  2:3818842/1‑150 (MQ=255)
                                                                                                  ctcAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCCGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAAACGCGTTAATAAATTATTTGATAAATGAATCCAg                                                     >  2:8518105/1‑150 (MQ=255)
                                                                                                   tcAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATTATAAATCGCCGAGGGGGGTGGGAATGTGCAGCGCACGCGGTGTAAACGAGGGAAAACCGCAGTTTTAAAAGCGCGGTTAAAAATTATTTTTTATATTAATCCacc                                                    >  2:5746239/1‑148 (MQ=255)
                                                                                                     aGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCAc                                                  <  2:1790866/150‑1 (MQ=255)
                                                                                                        cTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCtt                                               <  1:10012456/150‑1 (MQ=255)
                                                                                                          tCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGAAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCa                                             <  2:4751566/150‑1 (MQ=255)
                                                                                                              gCAGAAGGGCACCCCGAGTCGTTTGGTTTCGATTATAAATAGCAGAGGAGGTTGGTAATTTGCAGCGCACCCGTTGTAAAAGATGAAAAAAGGCAGTTTTACAAAGGCGTTAATAAAATATTTTATATATTAATACAACACCTTAAGaaa                                         >  2:8274944/1‑149 (MQ=255)
                                                                                                                aGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTTTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGa                                       <  2:12026068/150‑1 (MQ=255)
                                                                                                                aGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGa                                       <  2:7176953/150‑1 (MQ=255)
                                                                                                                   aGGGCACCCCGAGTCGTTTGGTTGCGATGATAAAACGCAGAGGGGGATGGTAATGTCCAGCGCACGCGTTGTAAAAGAGGAAAAAACGCAGTTTTACAAACGCGTTAATAAATTATTTTATATATGAATACAGCACCTTCAGAACGACaa                                    >  2:4486550/1‑149 (MQ=255)
                                                                                                                        aCCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGa                               <  1:13493607/150‑1 (MQ=255)
                                                                                                                        aCCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGa                               <  1:9856599/150‑1 (MQ=255)
                                                                                                                           ccGAGTCGTTTGGTTTCGATGAAAAAACGCAGAGGAGGATGGTAAAGTCCAGCCCACGCCTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCATCAGAACGACATCCAGAtct                            >  2:12687823/1‑150 (MQ=255)
                                                                                                                            cGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGTAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGAtctt                           <  1:14369394/150‑1 (MQ=255)
                                                                                                                             gAGTCGTTTGGTTGCGATGATACATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGAtcttc                          <  1:8704286/150‑1 (MQ=255)
                                                                                                                             gAGTAGTTTGGTTGCAATGATATATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGAtcttc                          <  2:5021371/150‑1 (MQ=255)
                                                                                                                               gTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACTAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATcttctt                        <  2:13164084/150‑1 (MQ=255)
                                                                                                                               gTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATcttctt                        <  1:212616/150‑1 (MQ=255)
                                                                                                                                 cGTTTGGTTGCGATGATAAATCTCAGAGGACGATGGTAATGTCCAGCGCACGCGTTGAAAACGAGGAAAAACGGCAGATTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCa                      <  1:4474642/150‑1 (MQ=255)
                                                                                                                                 cGTTTGGTTGCGATGATAAATAGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCa                      <  2:15430234/150‑1 (MQ=255)
                                                                                                                                   tttGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACg                    >  2:14613793/1‑150 (MQ=255)
                                                                                                                                   tttGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACg                    >  2:7792741/1‑150 (MQ=255)
                                                                                                                                        ttGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTa               <  1:5672565/150‑1 (MQ=255)
                                                                                                                                           cGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCt            >  2:7390559/1‑150 (MQ=255)
                                                                                                                                            gatgatAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCGAGCACCTTGAGTACGACATCCAGATCTTCTTCAGGTTTTAGCTc           >  2:9363430/1‑150 (MQ=255)
                                                                                                                                            gatgatAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTc           <  1:13219342/150‑1 (MQ=255)
                                                                                                                                            gatgatAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTc           >  1:6017545/1‑150 (MQ=255)
                                                                                                                                             atgatAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCa          >  2:610324/1‑150 (MQ=255)
                                                                                                                                                  aaaTCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAATTTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATcccc     <  1:6252671/150‑1 (MQ=255)
                                                                                                                                                   aaTCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGt                   >  1:12249848/1‑135 (MQ=255)
                                                                                                                                                   aaTCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGt                   <  2:12249848/135‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTG  >  NC_000913/2185297‑2185595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: