Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,304,649 C→T 6.8% intergenic (+256/+459) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,6490CT 6.8% 1702.8 / 81.5 621intergenic (+256/+459)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base C (227/338);  new base T (14/27);  total (255/366)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 636 total aligned reads displayed.

GGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACG  >  NC_000913/2304500‑2304797
                                                                                                                                                     |                                                                                                                                                    
ggCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTc                                                                                                                                                      <  2:8736208/150‑1 (MQ=255)
 gCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCg                                                                                                                                                     >  1:5157186/1‑150 (MQ=255)
 gCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCg                                                                                                                                                     >  1:3076440/1‑150 (MQ=255)
 gCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCg                                                                                                                                                     <  1:1643733/150‑1 (MQ=255)
 gCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCAACCCCGTAGGTCg                                                                                                                                                     >  1:10049971/1‑150 (MQ=255)
   cTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGa                                                                                                                                                   <  1:3548583/150‑1 (MQ=255)
   cTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGa                                                                                                                                                   <  1:12820977/150‑1 (MQ=255)
   cTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCAGAGGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGa                                                                                                                                                   >  1:11440974/1‑150 (MQ=255)
   cTACACAGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGa                                                                                                                                                   <  2:4071375/150‑1 (MQ=255)
    tACACCGCTGTGAAGTGCGCCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTc                                                                                                                                                      <  1:14957125/146‑1 (MQ=255)
     acacCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATa                                                                                                                                                 >  1:10894692/1‑150 (MQ=255)
       acCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGCGCCGGAAGCCGGAGGCGACGCGGGCGCGTCTGCTCAGGCCTCCACCGCTGGGACGTGCGCCACCCCGTAGGTCGGagccg                                                                                                                                               >  2:15152148/1‑146 (MQ=11)
        ctgccgtgAAGTGCGGCACCCCGTAGGTCTGACAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGGGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCCCCCCGTAGGTTGGATAAgg                                                                                                                                              <  1:14305543/145‑1 (MQ=11)
        ccGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACGTCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAgg                                                                                                                                              <  2:743488/150‑1 (MQ=255)
        ccGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAgg                                                                                                                                              <  2:7215412/150‑1 (MQ=255)
        ccGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACAGCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAgg                                                                                                                                              <  2:6583067/150‑1 (MQ=255)
        ccGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTAACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAgg                                                                                                                                              <  1:7709991/150‑1 (MQ=255)
         cGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGAtgcggc                                                                                                                                             >  2:2844850/1‑145 (MQ=255)
         cGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGc                                                                                                                                             >  1:4502881/1‑150 (MQ=255)
          gccgtgAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCg                                                                                                                                            <  2:5904854/147‑1 (MQ=37)
          gCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCg                                                                                                                                            <  1:8436053/150‑1 (MQ=255)
           cagtgAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCgg                                                                                                                                           <  2:9708450/148‑1 (MQ=255)
           cTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCgg                                                                                                                                           <  2:13503011/150‑1 (MQ=40)
              tgAAGTGCTCCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTa                                                                                                                                        >  2:12382667/1‑150 (MQ=255)
              tgAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTa                                                                                                                                        <  2:6843614/150‑1 (MQ=40)
                aaGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcg                                                                                                                                      >  2:11623147/1‑150 (MQ=255)
                 aGTGCGGCACCCCGTAGGTCCGACAAGGCGGTCACGCCGCATCCGACATCAAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTAcgc                                                                                                                                     <  2:2454220/150‑1 (MQ=255)
                 aGTGCGGCACCCCGTAGGTCAGACAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgc                                                                                                                                     <  2:13395549/150‑1 (MQ=255)
                 aGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTAcgc                                                                                                                                     <  1:13411331/150‑1 (MQ=255)
                 aGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgc                                                                                                                                     >  1:5461686/1‑150 (MQ=255)
                 aGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgc                                                                                                                                     <  2:6387706/150‑1 (MQ=255)
                  gttcGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCAGCTGCGACGCGGCCGCGGCTGAACCGGCCTCCACCGCGGTGAAGTGCTCCACCCCGGCGGTCGGAGAAGGCGGTTCcgcc                                                                                                                                    >  2:13644768/4‑150 (MQ=14)
                  gTGCGGCACCCCGTAGGTCTGATAAGGCGGTCACGCCGCATCCGACATCCAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTAcgcc                                                                                                                                    <  1:14205345/150‑1 (MQ=255)
                  gTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCAACCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTAcgcc                                                                                                                                    <  2:10474485/150‑1 (MQ=255)
                   tGCGGCACCCCGTAGGTCAGACGAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTc                                                                                                                                                      >  1:15470307/1‑131 (MQ=21)
                   tGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgccg                                                                                                                                   >  2:14205282/1‑150 (MQ=255)
                   tGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgccg                                                                                                                                   <  2:15569941/150‑1 (MQ=255)
                     cGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACTGGAGAGAAGTGGTCAACCCCGAAGGACGGAGACGGCGGGGACGCCGCa                                                                                                                                 >  1:10215381/1‑150 (MQ=11)
                     cGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTc                                                                                                                                                      >  1:14565102/1‑129 (MQ=35)
                     cGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCa                                                                                                                                 >  1:884876/1‑150 (MQ=255)
                      ggCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGa                                                                                                                                                   >  1:2604435/1‑131 (MQ=37)
                      ggCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCAt                                                                                                                                >  1:5926498/1‑150 (MQ=255)
                      ggCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTAGGGTGAGGGGGTTAcgccgcct                                                                                                                                >  1:9325278/1‑148 (MQ=255)
                          ccccGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGa                                                                                                                            <  2:11811068/150‑1 (MQ=255)
                          ccccGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGa                                                                                                                            <  2:1816112/150‑1 (MQ=255)
                             cGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACAt                                                                                                                         >  1:7961165/1‑150 (MQ=255)
                              gTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGTGGCGTGTTTTCAGGACTACACCGCTGTGAAGTGCTGCACACCGTTGGTAGGATAAGGGGGTTAAGCCGCATCCGACATc                                                                                                                        >  1:5692997/1‑150 (MQ=11)
                                  gTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCCACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAg                                                                                                                                               <  2:14911530/123‑1 (MQ=255)
                                  gTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGACTAATAAGGCCTACACCCCAGTGAAGTGCTCCACCCCGGAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAAc                                                                                                                    >  1:419924/1‑150 (MQ=14)
                                   tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg                                                                                                                   <  2:5664606/150‑1 (MQ=39)
                                   tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCATATCAGGACAACACCGCTGTGAAGAGCTCCACCCCGAAGGTCTGAAAAGCCGGGGACGCCGCATACGACATCCAACg                                                                                                                   >  1:2289720/1‑150 (MQ=11)
                                   tCAGACAAGGCGGTCACGCCGCATCCGACATACAACGCCCGAGACGGTTGCATGTAGCGACGCAGGCGCCGAGTAGCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGGTAAGCCGGTTACGCCGCATCCGACagagacgc                                                                                                                   >  1:9204399/1‑143 (MQ=14)
                                    cAGACAAGGCGGTCACGCCGCATCCGTCATCCTCCGCCCGAGCCGGTTGCCTGAGGCGACGCTGGCGCGTCTTATCTGGCCTACACCGCGGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGc                                                                                                                  <  2:4961512/150‑1 (MQ=11)
                                        tacggCGGTCACGCCGCCTCCGACATCCAACGCCCGAGCCGGTGGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCaa                                                                                                              <  2:6680759/147‑1 (MQ=255)
                                        cccgccGGACACGCCGCACCCGACATCCAACGCCCGCGCCGGTTGCCTGTTGCGACGCTGTCGAGTCTTATCCGGCTTAGACCTTTGTTATGTGGTCCACCCGTTTGGTCTTATTATGTGGTTACGCCGCATCCGACATCTAACGCCCaa                                                                                                              <  1:6406238/145‑1 (MQ=2)
                                         aaGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCAt                                                                                                                                >  2:1275824/1‑131 (MQ=34)
                                          aGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCAt                                                                                                                                <  2:14267867/130‑1 (MQ=25)
                                          aGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATc                                                                                                                        >  1:3798111/1‑138 (MQ=34)
                                          aGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGc                                                                                                            <  2:13161092/150‑1 (MQ=34)
                                          aGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGc                                                                                                            >  2:10100502/1‑150 (MQ=34)
                                          aGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGc                                                                                                            <  2:15462332/150‑1 (MQ=34)
                                          aGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCGGAGGCGACGCTGGCGCGGCTTATCAGGCCGACCCCGCGGTGAAGTGCGCCCCCCCGTAGGTCGGCGAAGGCGGGGACGCCGCAGCCGCCCTCGAACGCCCCAGc                                                                                                            >  1:6680750/1‑150 (MQ=11)
                                               gTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATTCGACGCTGGCGCGTCTTATAAGGCCAACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAATGCGGTTAAGCCGCATACGACATCAAACGCCCAAGCCggat                                                                                                       >  1:863410/1‑148 (MQ=11)
                                               gTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGtt                                                                                                       <  1:11696550/150‑1 (MQ=34)
                                               gTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGACTGATGAGACGCAGGCGCGTCTAATCACGCCTACACAGCTGTGAAGTGCTCCACCCCGGAGGTCGGTTAAGGCGGGTACGCCGCGTCCGCCATCTACCGCCCCGGCCGGtt                                                                                                       >  1:14888091/1‑150 (MQ=11)
                                                tCACGCCGCAGCCGACATCCAACGCCCGAGCCTGTTGCCTGATGCGACTCTGGCGCGTTTTATTTGGGCTTGTCGTCTGTGATTTTCTCCTCCCGGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCTAGCCGGTTg                                                                                                      <  1:1380221/150‑1 (MQ=11)
                                                  aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                    <  1:11195814/150‑1 (MQ=32)
                                                  aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                    <  2:8377430/150‑1 (MQ=32)
                                                  aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATAAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                    >  1:10601378/1‑150 (MQ=17)
                                                   cgccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCt                                                                                                   <  2:3347315/150‑1 (MQ=32)
                                                   cgccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCt                                                                                                   <  1:1476656/150‑1 (MQ=32)
                                                    gccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgcc                                                                                                                                    <  2:4813957/116‑1 (MQ=32)
                                                     ccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgc                                                                                                                                     <  2:3058690/114‑1 (MQ=32)
                                                     ccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                                                                                                 >  2:11340448/1‑150 (MQ=32)
                                                      cgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACGTCTAACGCCCAAGCCGGTTGCCTGAt                                                                                                <  1:12138578/150‑1 (MQ=17)
                                                      cgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGAGACGCTGGCGCGTCTTATCAGGCCTAAACCGCTGTGAAGTGGTAAACACCGTAGGTAGGATAATTCGGTTAAGCCGCATCCGACATCTAAAGCCCAAGCCGGTTGCCTGAt                                                                                                >  1:7225962/1‑150 (MQ=11)
                                                       gcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATg                                                                                               <  1:215031/150‑1 (MQ=32)
                                                         aTCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                                                                                                 >  2:12202483/1‑146 (MQ=32)
                                                         aTCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTACCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCg                                                                                             <  1:15229008/150‑1 (MQ=17)
                                                          tCCGACATCCAACGCCCGAGCCGGTTGCCTGATTCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGa                                                                                            <  2:1988796/150‑1 (MQ=17)
                                                           ccGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGAc                                                                                           <  2:9374883/150‑1 (MQ=32)
                                                           ccGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGAc                                                                                           <  1:13015923/150‑1 (MQ=32)
                                                             gACATCCAACGCCCGAGCCGGTTGCCTGTTGCGCCGCTGGCGCGTCTTATCAGGCCGACACCCCGGTGTAGTGCTCCACCCCCTAGGTGGGATAATGCGGTTACGCCGCCTCCGACCGCAAACCCCCAAGCCGGTTGCCAGATGCGACGc                                                                                         >  1:4835546/1‑150 (MQ=11)
                                                               cATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTg                                                                                       <  2:11052888/150‑1 (MQ=32)
                                                                aTCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTgg                                                                                      <  2:3696735/150‑1 (MQ=32)
                                                                 tCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgc                                                                                     >  2:7769962/1‑150 (MQ=32)
                                                                   ccaCGCGCGAGCCGGTTGCGTGATGCGACGCTGGCGCGTCTGATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                   <  2:8955481/148‑1 (MQ=9)
                                                                   cAACGCCCGAGCCGGTTTCCTGATGCGACGCTGGCGCGTCTTATAAGGCCTACAACGCTGTGAAGTGCTCCACCCCGTAGGTAGGATAAGGCGGTTACGCCGCCTCCGACAACGAACGCCCAAGCCGGTTGCCTGGTGCGACGCTGgcgc                                                                                   >  1:1271858/1‑150 (MQ=11)
                                                                    aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGa                                                                                                                            <  1:12287986/108‑1 (MQ=32)
                                                                       gCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt                                                                               <  1:2824906/150‑1 (MQ=31)
                                                                       gCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt                                                                               <  1:9617433/150‑1 (MQ=31)
                                                                        cccGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCtt                                                                              <  2:5994749/150‑1 (MQ=11)
                                                                                                                                          aCCCCGTAGGTCTGACAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCg            <  1:7321018/150‑1 (MQ=11)
                                                                                                                                           ccccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGa           >  2:8141021/1‑150 (MQ=31)
                                                                                                                                           ccccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGa           <  2:5335494/150‑1 (MQ=31)
                                                                                                                                                tAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATcc      <  2:2613355/150‑1 (MQ=17)
                                                                                                                                                  ggTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCaa    <  2:4402870/150‑1 (MQ=34)
                                                                                                                                                   gTCGGATAAGGCGGTTTCGCCGCATGCTAGTTCTTACGCCCTAGCCGTTTGCCTGATGCGACTCTGGCGCGTCTTTTCTGGCCTAAACCGCTGTGAAGTGCTCCACCCCGTAGGTTTGATAAGGCGGTTACGCCGCATCCGACATCCAAc   <  1:6828614/150‑1 (MQ=11)
                                                                                                                                                   gTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCAGCATCCGACATCCAAc   >  2:9199282/1‑150 (MQ=21)
                                                                                                                                                    tCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACg  >  2:14661581/1‑150 (MQ=34)
                                                                                                                                                     |                                                                                                                                                    
GGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACG  >  NC_000913/2304500‑2304797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: