Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,304,688 A→C 5.6% intergenic (+295/+420) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,6880AC 5.6% 868.6 / 5.8 375intergenic (+295/+420)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base A (121/194);  new base C (4/19);  total (130/245)
Fisher's exact test for biased strand distribution p-value = 4.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 392 total aligned reads displayed.

ACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATC  >  NC_000913/2304539‑2304837
                                                                                                                                                     |                                                                                                                                                     
aCAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCa                                                                                                                                                       >  2:11810462/1‑150 (MQ=35)
 taaGGCGGTCACGCCGCATCCGACATCCAACGGCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCaa                                                                                                                                                      <  2:6680750/149‑1 (MQ=14)
 cAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTATTATCAGGCCTAAACCGCTGTGATGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCaa                                                                                                                                                      <  1:6351441/150‑1 (MQ=255)
   aGGCGGTCACGCCGCATCCGACATCCACCGCCCGAGCGGGTTGCCTGATGCGACGGTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATATGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGc                                                                                                                                                    <  1:14866908/150‑1 (MQ=255)
   aGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGc                                                                                                                                                    <  2:12988720/150‑1 (MQ=34)
   aGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGc                                                                                                                                                    >  2:10100502/1‑150 (MQ=34)
   aGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGc                                                                                                                                                    <  1:9004842/150‑1 (MQ=34)
   aGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGc                                                                                                                                                    <  2:5518010/150‑1 (MQ=34)
    ggCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGcc                                                                                                                                                   >  1:1877153/1‑150 (MQ=34)
     gCGGTCACGCCGCATCCGACATCCAACGGCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                                                                                                                                                  >  1:14410107/1‑150 (MQ=17)
     gCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCTGGCCTACACCGCTGTGAAGTGCTCCACCCCGTTGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                                                                                                                                                  <  1:1075343/150‑1 (MQ=21)
     gCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                                                                                                                                                  <  2:13553576/150‑1 (MQ=34)
     ccGGACACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGTCGCTGGCGCGTCTTATCAGGCCTACACCGGTGTTAAGTGGTCCACCCCGTAGGTCGGATTAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                                                                                                                                                  <  2:14888091/149‑1 (MQ=11)
        gTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGtt                                                                                                                                               <  1:10575216/150‑1 (MQ=34)
        gTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGtt                                                                                                                                               <  1:15378175/150‑1 (MQ=34)
         tCACGCCGCAGCCGACATCCAACGCCCGAGCCTGTTGCCTGATGCGACTCTGGCGCGTTTTATTTGGGCTTGTCGTCTGTGATTTTCTCCTCCCGGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCTAGCCGGTTg                                                                                                                                              <  1:1380221/150‑1 (MQ=11)
          cACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGc                                                                                                                                             <  1:9285007/150‑1 (MQ=32)
          cACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGc                                                                                                                                             <  1:2443459/150‑1 (MQ=32)
           aCGCCGCATCCGACATCCCACGCCCGAGCGGTTTGCCTGATGCGACTCTGGCGCGTCTTATCTGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                                                            <  1:1514751/150‑1 (MQ=11)
           aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCCGGCCTACACCGCTGTGATGTGCTCCACCCCGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                                                            <  1:4487586/150‑1 (MQ=12)
           aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                                                            <  2:3698090/150‑1 (MQ=32)
           aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                                                            <  1:11195814/150‑1 (MQ=32)
           aCGCCGCAGCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                                                            <  1:7761058/150‑1 (MQ=17)
            cgccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGTCGCTGGCGCGTCTTATCAGGCCTACTCCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCt                                                                                                                                           <  2:9162539/150‑1 (MQ=17)
            cgccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCACGCCGGTTGctt                                                                                                                                           <  2:5912388/150‑3 (MQ=17)
            cgccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCt                                                                                                                                           <  1:10359913/150‑1 (MQ=32)
             gccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTg                                                                                                                                          <  1:9161597/150‑1 (MQ=32)
             gccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCGGGCGCGTCTTATCAGGCCTACACCGCGGTGAAGTGCTCCACCCCGGAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCGAACGCCCCAGCCGGGTGCCTg                                                                                                                                          >  1:7492240/1‑150 (MQ=11)
              ccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                                                                                                                                         >  2:11340448/1‑150 (MQ=32)
              ccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                                                                                                                                         <  2:15559672/150‑1 (MQ=32)
              ccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                                                                                                                                         >  1:14903731/1‑150 (MQ=32)
                gcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATg                                                                                                                                       <  1:215031/150‑1 (MQ=32)
                gaaTCCGACAACCAACGCCCGAGCCCCTTTCCTTATCCTAAGATGTCTAGTCTTAACAGGCCTACACCGCAGTGAAGTGCTCCACCCCGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATg                                                                                                                                       <  2:15095697/148‑1 (MQ=11)
                   tCCGACATCCAACGCCCGAGCCGGTTGCCTGATTCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGa                                                                                                                                    <  2:1988796/150‑1 (MQ=17)
                   tCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGa                                                                                                                                    <  1:14737837/150‑1 (MQ=32)
                   tCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGa                                                                                                                                         >  2:933312/1‑145 (MQ=17)
                   tCCGACATCCAACGCCCGAGCCGGTTGCCGGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTGCACCCCGTAGGTCTGAGAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGa                                                                                                                                    <  1:8679086/150‑1 (MQ=255)
                    ccGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGAc                                                                                                                                   <  2:14063799/150‑1 (MQ=32)
                     cGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACg                                                                                                                                  >  2:14234343/1‑150 (MQ=32)
                     cGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACg                                                                                                                                  >  2:12311728/1‑150 (MQ=32)
                      gACATCCAACGCCCGAGCCGGTTGCCTGTTGCGCCGCTGGCGCGTCTTATCAGGCCGACACCCCGGTGTAGTGCTCCACCCCCTAGGTGGGATAATGCGGTTACGCCGCCTCCGACCGCAAACCCCCAAGCCGGTTGCCAGATGCGACGc                                                                                                                                 >  1:4835546/1‑150 (MQ=11)
                          tCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgc                                                                                                                             >  2:7769962/1‑150 (MQ=32)
                           ccATCGCGCGAGGCGTTTGCGTGATGCTACGCTGGCGCGTCTTTTGTGGGCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcg                                                                                                                            <  1:12516707/150‑1 (MQ=11)
                            cAACGCCCGAGCCTGTTGCCTGCTGCGTCGCTGGCGCGTCTTATCAGGCCTTCACCGCAGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTCACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                           <  2:3365440/150‑1 (MQ=11)
                            cAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                           <  2:5217402/150‑1 (MQ=32)
                            cAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                           >  2:7419438/1‑150 (MQ=32)
                             aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCACATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGcgcg                                                                                                                          >  2:6765890/1‑150 (MQ=17)
                             aaCGCCCGAGCCGGTTGCCAGATGCGACGCAGGCGCGTCTTATCAGGCCTACACCGCAGTGAAGTGCTACACCCCCTAGGTCGGATAAGGCGGTTACGCCGCAACCGACAACTAACGCCCAAGCCCGTAGCCTGATGCGACGCTGGcgcg                                                                                                                          >  1:12792049/1‑150 (MQ=11)
                              aCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                         <  2:5154234/150‑1 (MQ=32)
                              aCGCCCGAGCCGGTGGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGACAAGGCGGTAACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                         <  1:4073678/150‑1 (MQ=12)
                                gCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt                                                                                                                       <  1:9617433/150‑1 (MQ=31)
                                 cccGAGCCGGTTGCCTGATTCGACGCTGTCGCGTCTTATCTGGCCTACTCCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCTGTTGCCTGATGCGACGCTGGCGCGTCtt                                                                                                                      <  2:6453749/150‑1 (MQ=11)
                                 cccGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCtt                                                                                                                      <  1:1582047/150‑1 (MQ=11)
                                                                                                   aCCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCg                                                    >  1:12865794/1‑150 (MQ=11)
                                                                                                    ccccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGa                                                   <  2:8817811/150‑1 (MQ=31)
                                                                                                    ccccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGa                                                   <  2:8268501/150‑1 (MQ=31)
                                                                                                     cccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGAc                                                  <  2:2976663/150‑1 (MQ=32)
                                                                                                     cccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGAc                                                  >  2:13309031/1‑150 (MQ=32)
                                                                                                     cccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGAc                                                  >  1:7235726/1‑150 (MQ=32)
                                                                                                      ttagatgTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACa                                                 >  2:13446977/7‑150 (MQ=17)
                                                                                                      ccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCTCTGTGAAGTGCTCCACCCCGGAGGTTGGATAAGGCGGTTACGCCGCATCCGACa                                                 >  2:4840838/1‑150 (MQ=17)
                                                                                                      ccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACa                                                 >  2:11644689/1‑150 (MQ=32)
                                                                                                            gTCTGATATGGCGGTTACGCCGGATCCGACTTATAACGCACCAGCCGGTTGCCTGATGCTACTCTGTCGAGTCTTAACAGGGCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAAc                                           <  1:11883425/150‑1 (MQ=11)
                                                                                                            gTCGGATAAGGCGGTTTCGCCGCATGCTAGTTCTTACGCCCTAGCCGTTTGCCTGATGCGACTCTGGCGCGTCTTTTCTGGCCTAAACCGCTGTGAAGTGCTCCACCCCGTAGGTTTGATAAGGCGGTTACGCCGCATCCGACATCCAAc                                           <  1:6828614/150‑1 (MQ=11)
                                                                                                             tCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACg                                          >  2:14661581/1‑150 (MQ=34)
                                                                                                                 aTAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCaa                                            >  1:14204461/1‑144 (MQ=34)
                                                                                                                 aTAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCg                                      >  2:9726376/1‑150 (MQ=37)
                                                                                                                  tAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGa                                     <  2:4656604/150‑1 (MQ=37)
                                                                                                                    aGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGACTGTTGCGACGCTGGCGCGTATTATCAGTCCTACACCGCTGTGATGTGCTCCTCACGGCAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGc                                   <  2:10891760/150‑1 (MQ=11)
                                                                                                                      gCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                                 <  1:6722745/150‑1 (MQ=37)
                                                                                                                      gCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTGGGATAAGGCGGGTACGCCGCATCCGACCGCCAACGCCCGAGCCg                                 >  1:3297028/1‑150 (MQ=11)
                                                                                                                        ggTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGt                               <  2:12597562/150‑1 (MQ=37)
                                                                                                                         gtgaCGCCGCATCCGACATCTAACGCCCCTGCCGGTTGCCTGTTGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTGGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGtt                              <  1:13644768/147‑1 (MQ=11)
                                                                                                                          tcaCGCAGCATACGAGATCTTTCGCGCGAGCGGTTTGCGTGATTCGACTCTGGCGCGTCTTATCTGGGCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTGGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTg                             <  2:6992612/148‑1 (MQ=11)
                                                                                                                             cgccgcATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCt                          <  1:10039079/150‑1 (MQ=37)
                                                                                                                              gccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTg                         >  1:1523302/1‑150 (MQ=37)
                                                                                                                               ccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCATCGTCCGAGCCGGTTGCCTGa                        >  2:9407911/1‑150 (MQ=37)
                                                                                                                                cgcATCCGACATCTAACGCCCAATCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGAt                       >  1:14121184/1‑150 (MQ=37)
                                                                                                                                 gcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATg                      <  2:10387134/150‑1 (MQ=37)
                                                                                                                                 gcAACCGACATCTAACGCCCTAGCCGGTGGCCTGCTGCGTCGCTGTAGCGTCTTATCATGCCTACACCGCTGTGACGTGCTCCACCGAGCTGGTCCGATAAGGCGGTCACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATg                      <  2:419924/150‑1 (MQ=11)
                                                                                                                                  cATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGc                     >  1:2810457/1‑150 (MQ=37)
                                                                                                                                    tcagACATCTATCGCCCTATCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGa                   <  1:12092596/147‑1 (MQ=255)
                                                                                                                                     ccGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGAc                  >  2:15322115/1‑150 (MQ=37)
                                                                                                                                      cGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACg                 >  1:5680018/1‑150 (MQ=37)
                                                                                                                                      cGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACg                 >  2:11097675/1‑150 (MQ=37)
                                                                                                                                       accttatAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGc                <  1:5632314/144‑1 (MQ=255)
                                                                                                                                         cATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTg              <  1:9940943/150‑1 (MQ=37)
                                                                                                                                           tCTACCGCCGCAGGCCGTCGCCTGCTGCGCCGGTGGCGCGTCCGATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgc            <  2:8464865/150‑1 (MQ=11)
                                                                                                                                            cTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGTTTACGCCGAATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcg           >  2:613963/1‑150 (MQ=255)
                                                                                                                                            cTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcg           <  2:2444938/150‑1 (MQ=37)
                                                                                                                                             tAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTGGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcgc          <  2:1795431/150‑1 (MQ=255)
                                                                                                                                             tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcgc          >  1:13285188/1‑150 (MQ=37)
                                                                                                                                             tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGAAAACCCACATCCGGAGTGCTCAACCCCGTAGTGTGGGGAAGGAGTTTTCGACGAATCCGACATCAAACGGCCGAGCCGGTGGCATGATGCGGCGCTGgcgc          >  2:1393266/1‑150 (MQ=2)
                                                                                                                                               aCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGt                                         >  2:8104582/1‑117 (MQ=37)
                                                                                                                                                cGCCCCAGCCGGTTGCCTGATGCGTCGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTc       <  2:12749192/150‑1 (MQ=255)
                                                                                                                                                cGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTc       <  1:8232510/150‑1 (MQ=37)
                                                                                                                                                cGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTc       <  1:35852/150‑1 (MQ=37)
                                                                                                                                                cGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTc       <  2:2670966/150‑1 (MQ=37)
                                                                                                                                                    cAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt   <  1:7200708/150‑1 (MQ=37)
                                                                                                                                                    cAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt   <  1:11380152/150‑1 (MQ=37)
                                                                                                                                                     |                                                                                                                                                     
ACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATC  >  NC_000913/2304539‑2304837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: