Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,460,555 A→T 5.3% intergenic (‑86/+95) fadI ← / ← yfcZ beta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,460,5550AT 5.3% 2380.4 / 13.6 804intergenic (‑86/+95)fadI/yfcZbeta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein
Reads supporting (aligned to +/- strand):  ref base A (398/293);  new base T (2/66);  total (405/399)
Fisher's exact test for biased strand distribution p-value = 1.48e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 850 total aligned reads displayed.

GTAAACCGCTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAA  >  NC_000913/2460406‑2460704
                                                                                                                                                     |                                                                                                                                                     
gtaAACCGCTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGaaaa                                                                                                                                                       <  1:6671126/150‑1 (MQ=255)
   aaCCGCTAACAATGGCGATACGATCGCCCTCGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAggg                                                                                                                                                    >  2:6170936/1‑150 (MQ=255)
      cGCTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGaaa                                                                                                                                                 <  1:10403212/150‑1 (MQ=255)
      cGCTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGaaa                                                                                                                                                 >  1:7372723/1‑150 (MQ=255)
      cGCTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGaaa                                                                                                                                                 <  2:2241886/150‑1 (MQ=255)
        cTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCACGGGGAGAAAAGGGCAAGt                                                                                                                                               >  1:11339051/1‑150 (MQ=255)
        cTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGt                                                                                                                                               >  2:6023592/1‑150 (MQ=255)
         tAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTg                                                                                                                                              >  2:12227038/1‑150 (MQ=255)
         tAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTg                                                                                                                                              >  2:3587344/1‑150 (MQ=255)
          aaCAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTgg                                                                                                                                             <  2:5622063/150‑1 (MQ=255)
           aCAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTggg                                                                                                                                            <  2:10535597/150‑1 (MQ=255)
           aCAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTggg                                                                                                                                            <  2:4559939/150‑1 (MQ=255)
            cAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGa                                                                                                                                           <  1:12102066/150‑1 (MQ=255)
             aaTGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAg                                                                                                                                          <  2:13790951/150‑1 (MQ=255)
              aTGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGc                                                                                                                                         <  1:8758463/150‑1 (MQ=255)
               tGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCt                                                                                                                                        <  1:10222579/150‑1 (MQ=255)
                   gATACGATAGCCCTGGCGGGTAACCAGCTGTAAAACCTGAACAATAACACTCCCCTGTAAAAAAATAAATAAATTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGa                                                                                                                                    <  2:3680276/150‑1 (MQ=255)
                     tACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAACTAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGaca                                                                                                                                  <  2:12457044/150‑1 (MQ=255)
                        gATCGCCCTGGCGGGTACCCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCCAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACag                                                                                                                               >  2:5281756/1‑150 (MQ=255)
                        gATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACag                                                                                                                               >  1:6366857/1‑150 (MQ=255)
                         aTCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACaga                                                                                                                              >  2:10238953/1‑150 (MQ=255)
                         aTCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACaga                                                                                                                              >  1:8586198/1‑150 (MQ=255)
                            gCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACagagag                                                                                                                           >  1:9341145/1‑150 (MQ=255)
                             cccTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAgagaga                                                                                                                          <  2:10073486/150‑1 (MQ=255)
                               cTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGaaa                                                                                                                        <  1:3404346/150‑1 (MQ=255)
                               cTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGaaa                                                                                                                        <  2:12880668/150‑1 (MQ=255)
                                tGGCGGGTAACCAGCGGTAAAACCTGACCCATATCACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGaaaa                                                                                                                       <  2:5820601/150‑1 (MQ=255)
                                tGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGaaaa                                                                                                                       <  1:5630947/150‑1 (MQ=255)
                                    gggTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAaagaag                                                                                                                   >  1:15196977/1‑150 (MQ=255)
                                           cAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaa                                                                                                            >  1:15325249/1‑150 (MQ=255)
                                            aGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaaa                                                                                                           >  2:4041846/1‑150 (MQ=255)
                                            aGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaaa                                                                                                           >  1:1937133/1‑150 (MQ=255)
                                             gCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaaaa                                                                                                          <  1:6251/150‑1 (MQ=255)
                                             gCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaaaa                                                                                                          <  1:10730547/150‑1 (MQ=255)
                                                gTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaaaagaa                                                                                                       >  1:4963509/1‑150 (MQ=255)
                                                  aaaaCCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTTGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAgaaaagaaaa                                                                                                     <  2:12560026/150‑1 (MQ=255)
                                                  aaaaCCTGACCAATAACACTCCCCTTTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAgaaaagaaaa                                                                                                     <  1:443665/150‑1 (MQ=255)
                                                  aaaaCCTGACCAATAACACTCCCCTGTAAAAAAATAAATAAAGTTGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGACAAGTGGAGAAAATGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAgaaaagaaaa                                                                                                     <  2:341701/150‑1 (MQ=255)
                                                   aaaCCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaaaagaaatc                                                                                                    >  1:10001909/1‑148 (MQ=255)
                                                   aaaCCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAAc                                                                                                    >  1:4049772/1‑150 (MQ=255)
                                                     aCCTGACCCATAACACTCCCCTTTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGc                                                                                                  <  2:6526697/150‑1 (MQ=255)
                                                     aCCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGc                                                                                                  >  1:6964150/1‑150 (MQ=255)
                                                     aCCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGc                                                                                                  <  2:2436515/150‑1 (MQ=255)
                                                          aCCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGTAATGTGGGAGCTATGACACAGATAGAAAAGAAGAAGATAAAAGAAAACGCCCCTg                                                                                             <  2:15281095/150‑1 (MQ=255)
                                                           aaaaTAAAACTACCATTTAAAAAAATAAATAATGTGTTCTTACCTTATAATTTTATTAAAAATTTTTTTAAATTTAGCAAAGTGTATAAATGGGAATGTGGGAGCTATTACACAGATATAAAAGAATAAGAGAAAAGAAAACGCCCCTGc                                                                                            <  2:9599161/147‑1 (MQ=255)
                                                            ccATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGcc                                                                                           >  1:3197037/1‑150 (MQ=255)
                                                            ccATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGcc                                                                                           >  1:6014121/1‑150 (MQ=255)
                                                                 acacTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTATTAACCATTTTTTTACATTTAGCCATGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCaa                                                                                      <  2:8935780/150‑1 (MQ=255)
                                                                     tCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAACGAAAACGCCCCTGCCAGCAACTGa                                                                                  <  2:1312568/150‑1 (MQ=255)
                                                                     tCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGa                                                                                  >  1:2302811/1‑150 (MQ=255)
                                                                       cccttaaaaaaaaTAAATACTGTGGTCTGCCCTTATCTTTTTATTAACATTTTTTTTACTTTTCGCCAGGTGTTGAAATGGTAATGGGGGTGCTTTGACACCGAGACAAAAGAACACGACAAAAAAACACGCCCCTGCCAGCAACTGACa                                                                                <  2:6608301/144‑1 (MQ=255)
                                                                        ccTTTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAg                                                                               <  2:546439/150‑1 (MQ=255)
                                                                        ccTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAg                                                                               <  2:11625760/150‑1 (MQ=255)
                                                                         cTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAgg                                                                              >  1:9822649/1‑150 (MQ=255)
                                                                          tGTAAAAAAATAAATACAGTGGTCTGACCTGATAATAGTATTAACCATTTTTTTACATTTAGCCATGTGGAGAACCGGGATTGTGGGAGCTATGACACAGAGAGAAAAGAAGACGCGAAACGAACACGCCCCTGTCAGTTGCTGGCAggg                                                                             <  2:1937133/150‑1 (MQ=255)
                                                                            tAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGc                                                                           >  2:14293992/1‑150 (MQ=255)
                                                                                 aaataaatAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcgtacg                                                                      >  1:14201999/1‑150 (MQ=255)
                                                                                      aataaaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTAccc                                                                 >  1:10989552/1‑150 (MQ=255)
                                                                                      aataaaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTAccc                                                                 <  2:1203822/150‑1 (MQ=255)
                                                                                       ataaaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCg                                                                >  2:4385474/1‑150 (MQ=255)
                                                                                        taacGTGGTCTGACCTTATATTTTTCTTAACATTTTTTTTACATTTAGCCATGTGGAGAAATGGTAATGTGGTTGCTATGACACAGATATAAAACAATAAGAGAAAACAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTAcccaa                                                               <  2:7212117/150‑3 (MQ=255)
                                                                                          aaGTGGTCTGACCGGATCTTTGTCTTAACCATTTTTTTACATTTAGCCACGTGGAGAAAAGGTAATGTGGTAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAACAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCgaga                                                             <  2:7486250/150‑1 (MQ=255)
                                                                                           aGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGagag                                                            >  2:14711562/1‑150 (MQ=255)
                                                                                             tGGTCTGACCTGATTTTTTTTTTAACCTTTTTTTTACATTTAGCCATGTGGAGATACGGTAATGTGGGTGGTATGACACCGATTGAATAGAAGAAGATATCAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGATGa                                                          <  2:1718516/150‑1 (MQ=255)
                                                                                               gTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGAcaca                                                                                                                                <  1:547029/78‑1 (MQ=255)
                                                                                                     ccTTATAATAGTCTTAACAATTTTTTTAAATTTAGCCATGTGGAGAATTGGGAATGTGGGAGATATGACACAGAGATAAAAGAAGAAGAGAAAATAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGc                                                  <  2:7569955/150‑1 (MQ=255)
                                                                                                      cTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAaagaagaa                                                                                                                 >  1:2805511/1‑86 (MQ=255)
                                                                                                      cTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCgtacgtac                                                                   <  2:14137822/132‑1 (MQ=255)
                                                                                                      cTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCa                                                 >  2:15556017/1‑150 (MQ=255)
                                                                                                      cTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCa                                                 >  1:13090777/1‑150 (MQ=255)
                                                                                                             tattattAACAATTTTTTTACATTTAGCCAAGTGGATAAAAGGTAATGTGGGAGCTATGACACAGAGATAAAAGAATAATAGAAAACAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAg                                          <  1:12702695/145‑1 (MQ=255)
                                                                                                              tgTCTTAACCATTTTTTTAAATTTAGCCACGTGGAGAAAAGGGAAAGTGGGAGTTATGACACAGAGAGAAAAGAAGAATAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGc                                         <  2:8237808/149‑1 (MQ=255)
                                                                                                              aGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGc                                         <  2:11521132/150‑1 (MQ=255)
                                                                                                               ttattAACCTTTTTTTTAAATTTAGCAATGGGTATAAATGGTAATTTGTGAGATTTTAAACATATATAAAATAATAACATAAAATAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCt                                        <  1:906146/147‑1 (MQ=255)
                                                                                                                  ttAACCATTTTTTTAAATTTAGCCATGTGGAGAAAAGGTAATGTGGGAGATATGACACAGAGAGAAAAGAAGAAGATAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGa                                     <  2:8608447/150‑1 (MQ=255)
                                                                                                                  ttAACATTTTTTTTACTTTTTGCCATGTGGCTAAATGGTATTGGGGGCGCTATTACACTGAGAGAAAATATGATGATAAAATAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGa                                     <  1:13530961/150‑1 (MQ=255)
                                                                                                                    aaCCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaaa                                   >  2:13548425/1‑150 (MQ=255)
                                                                                                                    aaCCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaaa                                   >  2:15073029/1‑150 (MQ=255)
                                                                                                                     acaaTTTTTTTACATTTAGCCATGTGGATAATTGTTAATGTGGGATCTAGGACACAGACACAAACTAATAATATAAAACAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaaaa                                  <  1:1552764/147‑1 (MQ=255)
                                                                                                                     acaaTTTTTTAAAATTTAGCAAAGTGTACAAAAGTGAAAGTGGTACCTATCACACAGACACAAAACAAAAACAAAAAACAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaaaa                                  <  1:4676656/147‑1 (MQ=255)
                                                                                                                       cATTTTTTAACATTCACCCTTGGTGACAAACGCCAAAGTGGGAGCAATGACACACAGAAAAAATAACAAGATAAAACACAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATc                                <  1:5279804/150‑1 (MQ=255)
                                                                                                                        aTTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCa                               >  2:4847944/1‑150 (MQ=255)
                                                                                                                        aTTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCa                               >  1:14487318/1‑150 (MQ=255)
                                                                                                                         tttttttACATTTAGCCATGTTGATAAAAGGTAATTTGGTATATATTACACAGAGATAAAACAAGAAGATAAAAGAAAACGCCCCTGCCAGCAACGGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAg                              <  2:14467754/150‑1 (MQ=255)
                                                                                                                               taaaTTTAGCCAAGTGCAGAAACGGTAACGTGGGAGCTATCACACATAGATAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                        <  1:13964487/147‑1 (MQ=255)
                                                                                                                               tACATTTAGCCAAGTGGAGAAAAGGGAATGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAACAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                        <  2:10706444/150‑1 (MQ=255)
                                                                                                                                   tttAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCt                    >  1:2515839/1‑150 (MQ=255)
                                                                                                                                     tAGCCAAGTGTAGAAACGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTc                  <  2:775438/150‑1 (MQ=255)
                                                                                                                                      ccccACGTGTAGAAATGGTAATGTGGGAGCTATGACACAGACATAAAAGAACAAGAGAAAACAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCg                 <  1:8034306/148‑1 (MQ=255)
                                                                                                                                       gCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGc                >  2:3165461/1‑150 (MQ=255)
                                                                                                                                        ccAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCa               >  1:3622737/1‑150 (MQ=255)
                                                                                                                                          acgtggtgAAACGGTAATGTGGGAGCTATTACACTTAGAGAAAATAAGAATAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAgg             <  1:7600157/148‑1 (MQ=255)
                                                                                                                                                agaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAg       >  1:11890388/1‑150 (MQ=255)
                                                                                                                                                 gaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGt      >  1:14845864/1‑150 (MQ=255)
                                                                                                                                                  aaaaGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCACAAGTg     >  2:10862107/1‑150 (MQ=255)
                                                                                                                                                  aaaaGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTg     >  2:6980646/1‑150 (MQ=255)
                                                                                                                                                    aaGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGACGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaa   >  2:11275293/1‑150 (MQ=255)
                                                                                                                                                    aaGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaa   >  2:9296659/1‑150 (MQ=255)
                                                                                                                                                     aGGTAATGTGGTAGCTATGACACAGAGAGAAACGAAGAAGAGAAAACACCACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaaa  <  2:2802630/150‑1 (MQ=255)
                                                                                                                                                     aGGTAAAGTGGGAGGTATGACACAGATAGAAAAGACGAACAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaaa  <  2:11339051/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTAAACCGCTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAA  >  NC_000913/2460406‑2460704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: