Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,460,558 G→T 6.7% intergenic (‑89/+92) fadI ← / ← yfcZ beta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,460,5580GT 6.7% 2710.1 / 34.3 819intergenic (‑89/+92)fadI/yfcZbeta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein
Reads supporting (aligned to +/- strand):  ref base G (414/315);  new base T (2/83);  total (416/403)
Fisher's exact test for biased strand distribution p-value = 1.46e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 864 total aligned reads displayed.

AACCGCTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCG  >  NC_000913/2460409‑2460707
                                                                                                                                                     |                                                                                                                                                     
 aCCGCTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGa                                                                                                                                                      <  2:4073225/150‑1 (MQ=255)
  ccGCTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCACGTGGAGAAAAGGGaa                                                                                                                                                     >  1:1399005/1‑150 (MQ=255)
   cGCTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCGAGAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGaaa                                                                                                                                                    <  2:9718884/150‑1 (MQ=255)
   cGCTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGaag                                                                                                                                                    >  1:9000974/1‑149 (MQ=255)
   cGCTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGaaa                                                                                                                                                    <  2:2242379/150‑1 (MQ=255)
   cGCTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGaaa                                                                                                                                                    >  2:1197624/1‑150 (MQ=255)
     cTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCACGGGGAGAAAAGGGCAAGt                                                                                                                                                  >  1:11339051/1‑150 (MQ=255)
      tAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAACGTg                                                                                                                                                 >  2:3587233/1‑150 (MQ=255)
        aCAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTggg                                                                                                                                               <  2:9416511/150‑1 (MQ=255)
         cAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGa                                                                                                                                              >  1:9599161/1‑150 (MQ=255)
            tGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCt                                                                                                                                           <  1:10222579/150‑1 (MQ=255)
            tGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCt                                                                                                                                           <  1:4558647/150‑1 (MQ=255)
             ggCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTa                                                                                                                                          <  2:1484078/150‑1 (MQ=255)
              gCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATACCACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTAt                                                                                                                                         <  2:444919/150‑1 (MQ=255)
                   aCGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATACAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGacac                                                                                                                                    <  1:4770549/150‑1 (MQ=255)
                     gATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCCTAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACag                                                                                                                                  >  2:15080363/1‑150 (MQ=255)
                     gATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACag                                                                                                                                  >  1:5798246/1‑150 (MQ=255)
                      aTCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACaga                                                                                                                                 >  2:12362371/1‑150 (MQ=255)
                      aTCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACaga                                                                                                                                 >  2:7495307/1‑150 (MQ=255)
                      aTCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACaga                                                                                                                                 <  1:3437728/150‑1 (MQ=255)
                       tCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGt                                                                                                                                                  <  2:3789440/132‑1 (MQ=255)
                       tCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGt                                                                                                                                                  >  1:3789440/1‑132 (MQ=255)
                       tCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGa                                                                                                                                       >  1:2683982/1‑143 (MQ=255)
                       tCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACagag                                                                                                                                >  2:9007097/1‑150 (MQ=255)
                        cGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACagaga                                                                                                                               <  1:1096468/150‑1 (MQ=255)
                         gCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACagagag                                                                                                                              >  2:10438741/1‑150 (MQ=255)
                           ccTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGaa                                                                                                                            >  2:5880394/1‑150 (MQ=255)
                             tGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGaaaa                                                                                                                          >  1:12493791/1‑150 (MQ=255)
                             tGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGaaaa                                                                                                                          <  2:13556611/150‑1 (MQ=255)
                             tGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGaaaa                                                                                                                          >  1:6551844/1‑150 (MQ=255)
                               gCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAaaga                                                                                                                        >  1:12052502/1‑150 (MQ=255)
                                   gTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTTTAAAAAAATAAATAAAGTGGTCTGACGTGATCATAGTCTTAACCTTTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAaagaagaa                                                                                                                    <  1:4229665/150‑1 (MQ=255)
                                   gTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAaagaagaa                                                                                                                    >  1:6157309/1‑150 (MQ=255)
                                   gTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAaagaagaa                                                                                                                    <  1:14922260/150‑1 (MQ=255)
                                    tAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAaagaagaag                                                                                                                   >  2:1749491/1‑150 (MQ=255)
                                      aCCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAagag                                                                                                                 >  1:1843740/1‑150 (MQ=255)
                                       ccAGCTGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAgaga                                                                                                                <  2:532250/150‑1 (MQ=255)
                                         aGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaaa                                                                                                              >  1:10706444/1‑150 (MQ=255)
                                         aGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaaa                                                                                                              >  1:629602/1‑150 (MQ=255)
                                            ggTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaaaaga                                                                                                           >  2:156539/1‑150 (MQ=255)
                                             gTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaaaagaa                                                                                                          >  2:5704177/1‑150 (MQ=255)
                                             gTAAAACCTGACCCATAACACTCCCCTGTAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaaaagaaa                                                                                                         >  1:11835937/1‑150 (MQ=255)
                                              tAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTAt                                                                                                                                         >  2:1218794/1‑118 (MQ=255)
                                              tAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaaaagaaa                                                                                                         <  2:6660840/150‑1 (MQ=255)
                                               aaaaCCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTTGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAgaaaagaaaa                                                                                                        <  2:12560026/150‑1 (MQ=255)
                                               aaaaCCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAgaaaagaaaa                                                                                                        >  1:13515615/1‑150 (MQ=255)
                                               aaaaCCTGACCCATAACACTCCACTTTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAgaaaagaaaa                                                                                                        <  2:8243018/150‑1 (MQ=255)
                                               aaaaCCTGACCAATAACACTCCCCTGTAAAAAAATAAATAAAGTTGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGACAAGTGGAGAAAATGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAgaaaagaaaa                                                                                                        <  2:341701/150‑1 (MQ=255)
                                                aaaCCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGATAAGGGTAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaaaagaaatc                                                                                                       >  1:8263588/1‑148 (MQ=255)
                                                aaaCCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaaaagaaatc                                                                                                       >  1:10001909/1‑148 (MQ=255)
                                                aaaCCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAAc                                                                                                       >  2:10035528/1‑150 (MQ=255)
                                                  aCCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGc                                                                                                     >  1:7255920/1‑150 (MQ=255)
                                                  aCCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGc                                                                                                     <  2:7372723/150‑1 (MQ=255)
                                                  aCCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGc                                                                                                     <  2:1020520/150‑1 (MQ=255)
                                                      gACCAATAACACTCCCCTTTAAAAAAATAAATAATGTGTTCTGACCTGATATTTGTCTTAACATTTTTTTTAAATTTTGCCATGTGGATAAATGGTAATGTGGGAGATTTGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCt                                                                                                 <  2:3112165/150‑1 (MQ=255)
                                                           aTAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGAGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAg                                                                                            >  2:7078548/1‑150 (MQ=255)
                                                                acTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGaca                                                                                                                                     >  1:2226288/1‑104 (MQ=255)
                                                                  tCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAACGAAAACGCCCCTGCCAGCAACTGa                                                                                     <  2:1312568/150‑1 (MQ=255)
                                                                  tCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAATCGCCCCTGCCAGCAACTGa                                                                                     >  2:6059471/1‑150 (MQ=255)
                                                                    cccTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACa                                                                                   >  1:5321573/1‑150 (MQ=255)
                                                                     ccTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCATGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAg                                                                                  <  2:6551844/150‑1 (MQ=255)
                                                                     ccTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAg                                                                                  <  2:2455421/150‑1 (MQ=255)
                                                                       tGTAAAAAAATAATTAAAGTGGTCTGAGCTTATCATATTCTTAACGTTTTTTTTAAATTTTGCCACGTTGTGAAATGGGATTGTGGGAGATTTGACACTGATTGAAAATAATAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAggg                                                                                <  2:11608166/150‑1 (MQ=255)
                                                                        ttAAAAAAATAAATAACGTGTTCTGACCTGATCAGTGTCTTAACCATTTTTTGAAATTTAGCAAAGTGCAGAACAGGTAAAGTGGGAGCTATGACACAGAGAGAAACGAAGACGACAAACGAAAACGCCCCTGCCAGCAACTGACAgggg                                                                               <  2:10626079/149‑1 (MQ=255)
                                                                              aaataaatAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcgtacg                                                                         >  1:14201999/1‑150 (MQ=255)
                                                                                       atgtggtgTGCCCTTATATTAGTCTTAACCTTTTTTTTACTTTTCGCCATGTGTTTAAACGGTAATGTGGGTGCTTTGACACTTAGAGAAATCAACAATCCAAAACAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCgaga                                                                <  2:14398501/148‑1 (MQ=255)
                                                                                            gTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAAt                                                           <  2:14166723/150‑1 (MQ=255)
                                                                                                gACGTGATCATTGTCTTAACCATTTTTTTACTTTTCGCCAGGTGGAGAAAAGGTATCGTGGGAGCTTTTACACAGATATAAAAGAATAAGCTAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAAc                                                       <  2:2790979/150‑1 (MQ=255)
                                                                                                gACCTGATCATTTTCTTAACAATTTTTTTACATTTAGCCAAGTGGAGAAATGGTAATGTGGGAGATATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAAc                                                       <  2:4963509/150‑1 (MQ=255)
                                                                                                gACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAAc                                                       >  1:8650244/1‑150 (MQ=255)
                                                                                                   cTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCa                                                    >  1:15365711/1‑150 (MQ=255)
                                                                                                        cATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGAccc                                               >  1:7503212/1‑150 (MQ=255)
                                                                                                         tttgTCTTAACTTTTTTTTTAATTTTTGCCTTGTGGTGACATGGGTATGTGGGTGCTATGACCCCGAGTGAAAATAAGAATACAAACTAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCa                                              <  1:3021258/147‑1 (MQ=255)
                                                                                                           aGTCTTAACCTTTTTTTTACATTTAGCCAAGTGTAGAAAAGGGAAAGTGGGAGCTTTGACACAGAGAGAAAATAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGc                                            <  2:8627236/150‑1 (MQ=255)
                                                                                                             tCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTg                                          >  2:2005391/1‑150 (MQ=255)
                                                                                                             tCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAAGTAACGCAGACCCAGCTg                                          >  1:12998359/1‑150 (MQ=255)
                                                                                                                 aaCCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaaa                                      >  2:10855933/1‑150 (MQ=255)
                                                                                                                  acaaTTTTTTTACATTTAGCCATGTGGATAATTGTTAATGTGGGATCTAGGACACAGACACAAACTAATAATATAAAACAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaaaa                                     <  1:1552764/147‑1 (MQ=255)
                                                                                                                  aCCTTTTTTTAACATTGAGCAATTTGGATAAACGGTAACGTGGGATCTAGCACACAGATCTAAACTAAGAATTTAAAACAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaaaa                                     <  1:5549204/150‑1 (MQ=255)
                                                                                                                   ccATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAAt                                    >  1:15240126/1‑150 (MQ=255)
                                                                                                                     aTTTTTTTACTTTTAGCCATGTTTAGAAATGGGAATGTGTGAGATATTACACAGCGATAAAAGAAGAGTAGAAAAGAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCa                                  <  1:14504770/150‑1 (MQ=255)
                                                                                                                     aTTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCa                                  >  1:6827564/1‑150 (MQ=255)
                                                                                                                     aTTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCa                                  >  2:5538461/1‑150 (MQ=255)
                                                                                                                      tttttttACATTTAGCCATGTTGATAAAAGGTAATTTGGTATATATTACACAGAGATAAAACAAGAAGATAAAAGAAAACGCCCCTGCCAGCAACGGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAg                                 <  2:14467754/150‑1 (MQ=255)
                                                                                                                              cATTTAGCCACGTGGAGAAAAGGGAATGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAg                         <  2:6260679/150‑1 (MQ=255)
                                                                                                                               aTTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCt                                           <  2:12939041/131‑1 (MQ=255)
                                                                                                                               aTTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGc                        >  1:325557/1‑150 (MQ=255)
                                                                                                                                   aGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAACAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCg                    >  2:11514804/1‑150 (MQ=255)
                                                                                                                                    gCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGc                   >  2:7619590/1‑150 (MQ=255)
                                                                                                                                       aaGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAgg                >  1:10453683/1‑150 (MQ=255)
                                                                                                                                        aGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGc               >  2:13880102/1‑150 (MQ=255)
                                                                                                                                          tGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaa             >  2:15309078/1‑150 (MQ=255)
                                                                                                                                            tataaaaGGTAATGTGGTATATATGACACAGAGAGAAAATAAGAAGACAAAAGAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaaaa           <  1:9234017/147‑1 (MQ=255)
                                                                                                                                            gagaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAACGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaaaa           <  2:4633973/150‑1 (MQ=255)
                                                                                                                                            gagaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAACGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaaaa           <  2:7881524/150‑1 (MQ=255)
                                                                                                                                               aaaaGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTg        >  1:2452312/1‑150 (MQ=255)
                                                                                                                                                 aaGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGACGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaa      >  2:11275293/1‑150 (MQ=255)
                                                                                                                                                  aGGTAAAGTGGGAGGTATGACACAGATAGAAAAGACGAACAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaaa     <  2:11339051/150‑1 (MQ=255)
                                                                                                                                                  aGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaaa     >  1:7728946/1‑150 (MQ=255)
                                                                                                                                                     gAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCg  >  1:5018905/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AACCGCTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCG  >  NC_000913/2460409‑2460707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: