Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,436 C→A 12.8% Q436K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4360CA12.8% 1756.6 / 61.1 583Q436K (CAA→AAA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base C (185/304);  new base A (89/5);  total (274/309)
Fisher's exact test for biased strand distribution p-value = 4.64e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 591 total aligned reads displayed.

TCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGT  >  NC_000913/2470288‑2470585
                                                                                                                                                    |                                                                                                                                                     
tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGACGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATCAAAGAAAACa                                                                                                                                                      >  2:7337318/1‑150 (MQ=255)
 ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                                                                                                                     <  2:7114690/150‑1 (MQ=255)
 ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                                                                                                                     <  1:12676017/150‑1 (MQ=255)
  tttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAg                                                                                                                                                    <  2:1602412/150‑1 (MQ=255)
  tttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAAACaac                                                                                                                                                    >  2:11135255/1‑149 (MQ=255)
     ttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACa                                                                                                                                                 <  1:2040926/150‑1 (MQ=255)
        gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGaaaaaa                                                                                                                                              >  2:11458442/1‑148 (MQ=255)
         aaTATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAATAAATAAAGAAAATAg                                                                                                                                             >  1:14993692/1‑150 (MQ=255)
            aacacAATTTTATGTCACAATATTCGCCCAAGCATATAGACAATTAAGCAAGCATTTATTTGGAATCAAAATTACGTTCGACCATTCCTTATACATAACAGTCGATTTTTAAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                                          <  2:5310258/146‑1 (MQ=255)
             tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                         <  2:3704202/150‑1 (MQ=255)
               tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAATAAAATATCGATGt                                                                                                                                       >  1:3894171/1‑150 (MQ=255)
               tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAATAAAAAAGCGATGt                                                                                                                                       >  1:11961896/1‑150 (MQ=255)
               tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGAAAGCATTTATTTGGAATGTAATTTTCTTTCGACCTATGCTTATACATAAAAGTCGTTTTTTTAATATAAAAAATAAAACAAAGAAATAAACACAAAAGAGATGt                                                                                                                                       >  2:15272267/1‑150 (MQ=255)
                cAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTa                                                                                                                                      <  1:4420305/150‑1 (MQ=255)
                 aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTaa                                                                                                                                     <  2:11683136/150‑1 (MQ=255)
                   ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                                   <  1:13637946/150‑1 (MQ=255)
                   ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                                   <  1:14244748/150‑1 (MQ=255)
                   ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                                   <  2:14570307/150‑1 (MQ=255)
                   tgctATGTGCCACTTATCGGGGAAGCATATAGAGATTTAAGCAACCATTTATTTGGAAGGCATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                                   <  2:15421685/147‑1 (MQ=255)
                      tATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTCAAAGAAAACAAGACAAAAGCGATGTatggcag                                                                                                                                >  1:12848647/1‑144 (MQ=255)
                       aTGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCAACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAATAATTAAAAAAAAAAAAAACAAAAGCGAAGTAAAGCAca                                                                                                                               >  1:5765126/1‑149 (MQ=255)
                          tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGAAAATATCGATGTAAAGCACTaat                                                                                                                            >  2:2067489/1‑149 (MQ=255)
                            tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAAAAGCGATGTAAAGCACTAAGtt                                                                                                                          >  2:5003492/1‑150 (MQ=255)
                            tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAATCAAGACAAAATCGGAGTAAAGCAAGAAGtt                                                                                                                          >  1:3423631/1‑150 (MQ=255)
                              aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAAAAAAAAACAAAAAAAAGATGTAAAGCAAAAAGTTTa                                                                                                                        >  1:13959173/1‑150 (MQ=255)
                                 tttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAAAAATTAAAAGAAATAAAGAAAAAATCGATGTAAAGAAATAAGTTTTAAt                                                                                                                     >  1:1857024/1‑150 (MQ=255)
                                      gggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAGAGCACTAAGTTTAAATTgcgc                                                                                                                <  2:665761/150‑1 (MQ=255)
                                         aaGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAAACAATACAAAATCGATGTAAAGCCAGAAGGTTAAATTTCGCGcc                                                                                                             >  1:15193093/1‑150 (MQ=255)
                                           ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAATCAAGACAATATCGATTTAAAGCACTAAGTTTAAATTGCGCGCCaa                                                                                                           >  2:13891726/1‑150 (MQ=255)
                                              tataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCatgat                                                                                                        <  2:5558757/150‑5 (MQ=255)
                                                  gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc                                                                                                    <  1:699115/150‑1 (MQ=255)
                                                  gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATAATGgc                                                                                                    >  2:11349234/1‑150 (MQ=255)
                                                  gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAATAAATAAAAACAAAAGAGATTTAAAGCAAAAATTTTTAATTTGGCGCCAAACAttgg                                                                                                    >  1:5937284/1‑147 (MQ=255)
                                                    gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAATACAAAAGCGATGTAAAGCACTAAGTTTAAATTTAGCGCCAATCATTgcgc                                                                                                  >  2:12082518/1‑150 (MQ=255)
                                                         aagcaagcATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAATAAAATAGCGATTTAAATCACTAAGTTTAAATTTAGCGCCAATAATTGCGCGcaca                                                                                             >  2:12533452/1‑150 (MQ=255)
                                                         aagaaagaATTTTTTTTTAATTTTTTTTTGTTTCGACCTTTTCTTTTAATTAAAAGTCTTTTTTTTAATTTATAAAATAATTCAAATAAATCATGAAAATAGCGATGTAAAGCAATATGTTTAATTTGCGCGCCATGATTGGCGCGcaat                                                                                             <  1:10360216/150‑3 (MQ=255)
                                                            caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGc                                                                                          <  2:4653505/150‑1 (MQ=255)
                                                             aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAAAATCGATGTAAATCACTAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGcg                                                                                         >  2:9443262/1‑149 (MQ=255)
                                                                      tttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCa                                                                                <  2:4515180/150‑1 (MQ=255)
                                                                        tGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAAc                                                                              <  2:8164424/150‑1 (MQ=255)
                                                                        tGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTAAAAAATAATTCAAAGAAATAAAGACAAAAGCCTTGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAAc                                                                              >  2:3330717/1‑150 (MQ=255)
                                                                               tATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAATAAAGAAAAAAGAGATTTAAAGCAAAAAATTTAAAATTAGCGCCAAAAAAGGCGCCCACAACCTATAATAACAAACTAAatt                                                                       >  2:3984194/1‑150 (MQ=255)
                                                                                aTTTTTCGTTCGACCTTTGCTTATACATAACAGTGTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAACACAATAGCTATGTAAAGAACTAATTTTAAATTGCGCGCCAATCTTGGCGCGCACAAGCTATAATACCAACCTAATTtc                                                                      <  1:7385167/150‑1 (MQ=255)
                                                                                 tttttCTTTCGACCTTTGATTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGAAAATAGCGTTGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtct                                                                     <  1:11349234/150‑1 (MQ=255)
                                                                                 tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATTTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtct                                                                     >  2:10303413/1‑150 (MQ=255)
                                                                                 tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAAAGCGATTTAAAGCCAGAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAATtct                                                                     >  2:6466652/1‑150 (MQ=255)
                                                                                  ttttCGTTCTACCTTTGCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                                    <  1:11151596/150‑1 (MQ=255)
                                                                                   tttCGGTCGACCTTTGCGTATACCTAACCGTCGTTTTTTTAATTTATAAAATCATTCAAAGAAATCAAGCCACTCGCGATGTAAAGCACTACGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATCCCAACCTAATTTctcc                                                                   <  2:12957663/150‑1 (MQ=255)
                                                                                        ttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAAAATCGATTTAAAGCACAAAGTTTAAATTTCGCCCCAATCAAGGCGCGCACAAGCTATAAAAACAAACAAATTTctcctcctc                                                              >  2:14242906/1‑150 (MQ=255)
                                                                                        ttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAAAAAGAAAAAAGCGATGTAAAGCACGAAGTTTAAATTTCGCGCCAATCAAGGCGCGCACAAGCAATAATAACAAACTAATTTatccaccac                                                              >  2:4367292/1‑148 (MQ=255)
                                                                                        ttcgACCTTTGCTTATAAATAACTGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCAAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcctc                                                              <  1:7788758/150‑1 (MQ=255)
                                                                                               tttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGt                                                       <  2:2723247/150‑1 (MQ=255)
                                                                                               tttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGt                                                       <  1:15469104/150‑1 (MQ=255)
                                                                                                 ttcTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGa                                                     <  1:3636103/148‑1 (MQ=255)
                                                                                                  gCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGAc                                                    <  1:6203881/150‑1 (MQ=255)
                                                                                                   cTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAAACAAGACAAAAGCGAAGTAAAGCACGAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAACTATAAAACCCACCAAAATTATACTCCGCTTAGAGTGACt                                                   >  2:10643283/1‑150 (MQ=255)
                                                                                                    ttATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCGTAGAGTGACta                                                  >  2:10453237/1‑150 (MQ=255)
                                                                                                    ttATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACta                                                  <  2:4596897/150‑1 (MQ=255)
                                                                                                          aTAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAAAGCGATGTAAAGCACTAAGTTTAAATTGCCCGCCAATCATGGCGCGCACAAGCGATAATACCAACCTAATTTCTCCTCCGCGTAGAGTGACTATAtcgc                                            >  2:8891126/1‑148 (MQ=255)
                                                                                                          aTAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAAACAAGACAAAAGCGATGTAAATCACTAAGTTTAAATTTCGCGCCAAACATGGCGCGCCCAAGCGATAATAACAACCAAATTTATCCACCTCTTAGAGTTAATATAtcta                                            >  2:6089876/1‑149 (MQ=255)
                                                                                                            aaCAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCt                                          <  2:12416308/150‑1 (MQ=255)
                                                                                                            aaCAGTCGTTTTTTTAATTTAAAAAAAAATTAAAAGAAATCAAGACAAAAGCGATGTAAAGCAAAAAGTTTAAAATTCGCGCCAATCATGGCGCGCACAAGCAATAATAACAACCTAATTTATACTCAGCATAGAGTGAATATATCACCt                                          >  2:7742951/1‑150 (MQ=255)
                                                                                                             aCAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTg                                         <  2:14909640/150‑1 (MQ=255)
                                                                                                                   gTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGaa                                   >  2:13759338/1‑150 (MQ=255)
                                                                                                                    tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcc                                                                <  2:9560822/120‑1 (MQ=255)
                                                                                                                       atatAATTTATAAAATAATTCAAAGAAAACAAGACAATAGCGAAGAACACCACTACGTATAAATTGCGCGACACTCCTGCCGCCCACAAGCTATAATACCAACCAAATTACTCCACCTCTTACAGTGACTATATCTCCTCATAGAATTGc                               >  1:10014881/4‑150 (MQ=255)
                                                                                                                        tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                              <  1:15289783/150‑1 (MQ=255)
                                                                                                                        tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                              <  2:6958494/150‑1 (MQ=255)
                                                                                                                          tAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGt                            <  1:8180029/150‑1 (MQ=255)
                                                                                                                          tAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                              >  2:2202756/1‑148 (MQ=255)
                                                                                                                           aaTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCACTCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTa                           >  1:3073969/1‑150 (MQ=255)
                                                                                                                             tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAtt                         <  2:4742525/150‑1 (MQ=255)
                                                                                                                             tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAtt                         <  2:9752952/150‑1 (MQ=255)
                                                                                                                               tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                       <  2:3001584/150‑1 (MQ=255)
                                                                                                                               tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                       <  2:9088200/150‑1 (MQ=255)
                                                                                                                                 taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTATTTTCTCCTCCTCTTCGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt                     >  1:288034/1‑150 (MQ=255)
                                                                                                                                 taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt                     <  2:5859400/150‑1 (MQ=255)
                                                                                                                                   aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTg                                >  1:8614349/1‑137 (MQ=255)
                                                                                                                                   aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt                   <  1:7186644/150‑1 (MQ=255)
                                                                                                                                   aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATATCTCATCCTCTTAGAGTGACTATATCTCCTGATAGAATTGATGTATTTACTAt                   >  1:9482184/1‑150 (MQ=255)
                                                                                                                                    aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTACAGGGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTTACTATATCTCCTGATAGAATTGCGGTATTGACTATc                  <  2:8272626/150‑1 (MQ=255)
                                                                                                                                    aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc                  >  1:2197273/1‑150 (MQ=255)
                                                                                                                                    aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc                  >  2:13310602/1‑150 (MQ=255)
                                                                                                                                     ataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCa                 >  1:2568034/1‑150 (MQ=255)
                                                                                                                                      taatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaa                <  2:8602301/150‑1 (MQ=255)
                                                                                                                                       aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa               <  1:4947139/150‑1 (MQ=255)
                                                                                                                                       aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa               <  1:9122287/150‑1 (MQ=255)
                                                                                                                                         ttCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg             <  1:6759026/150‑1 (MQ=255)
                                                                                                                                         ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg             >  1:1234129/1‑150 (MQ=255)
                                                                                                                                         ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg             >  1:9839106/1‑150 (MQ=255)
                                                                                                                                         ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg             >  2:2379913/1‑150 (MQ=255)
                                                                                                                                           cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc           <  2:3604412/150‑1 (MQ=255)
                                                                                                                                            aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc          <  2:1084929/150‑1 (MQ=255)
                                                                                                                                            aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc          <  1:14627681/150‑1 (MQ=255)
                                                                                                                                             aaGAAATCAAGACAATAGTGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt         <  1:13793377/150‑1 (MQ=255)
                                                                                                                                             aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt         >  1:8921711/1‑150 (MQ=255)
                                                                                                                                              aGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAttt                                                                       >  1:10802468/1‑87 (MQ=255)
                                                                                                                                               gAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGa       <  1:4731311/150‑1 (MQ=255)
                                                                                                                                                   tCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCg   >  1:2999605/1‑150 (MQ=255)
                                                                                                                                                    cAAGACAATAGCGATGTAAAGCACTAAGATTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgt  <  1:11539082/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGT  >  NC_000913/2470288‑2470585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: