Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,441 C→A 6.3% D437E (GAC→GAA gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4410CA 6.3% 1948.8 / 10.9 596D437E (GAC→GAAgtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base C (221/312);  new base A (59/4);  total (280/316)
Fisher's exact test for biased strand distribution p-value = 9.97e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 605 total aligned reads displayed.

TTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTT  >  NC_000913/2470292‑2470590
                                                                                                                                                     |                                                                                                                                                     
    gAATATCTCAATTTGTGGTGTAATTTTTCGGGGAAGGATATAGAGTTTTAAGCAAGCATTTATTTTGAATTTATTTTTCGTTCGACCTTTGCTTATACATTACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                                                                                                                   <  1:6794957/150‑1 (MQ=255)
       tatCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCg                                                                                                                                                <  2:9086615/150‑1 (MQ=255)
         tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGATTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                              <  1:5846523/150‑1 (MQ=255)
           tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTATTTTATTAAATAATTAAAATAAATCAAGAAAATAGGGAttt                                                                                                                                            >  1:1876053/1‑148 (MQ=255)
           tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGAAAGCATTTATTTGGAATGTAATTTTCTTTCGACCTATGCTTATACATAAAAGTCGTTTTTTTAATATAAAAAATAAAACAAAGAAATAAACACAAAAGAGATGt                                                                                                                                            >  2:15272267/1‑150 (MQ=255)
             aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTaa                                                                                                                                          <  1:11589323/150‑1 (MQ=255)
             aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGAAAATAGCGATGTaa                                                                                                                                          >  2:9521679/1‑150 (MQ=255)
               ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                                        <  1:13637946/150‑1 (MQ=255)
               ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                                        <  2:884361/150‑1 (MQ=255)
               tgctATGTGCCACTTATCGGGGAAGCATATAGAGATTTAAGCAACCATTTATTTGGAAGGCATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                                        <  2:15421685/147‑1 (MQ=255)
                 ttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCa                                                                                                                                      <  1:4146608/150‑1 (MQ=255)
                 ttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAATAAAAAAATACAAAAGCGATGTAAAgaa                                                                                                                                      >  2:13794669/1‑148 (MQ=255)
                  tATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTCAAAGAAAACAAGACAAAAGCGATGTatggcag                                                                                                                                     >  1:12848647/1‑144 (MQ=255)
                     gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTaa                                                                                                                                  <  1:3012958/150‑1 (MQ=255)
                     gtgtCAATTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGATTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTaa                                                                                                                                  <  2:7602851/150‑1 (MQ=255)
                      tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAg                                                                                                                                 <  2:14083334/150‑1 (MQ=255)
                       gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAATACAAAAGAGATGTAAAGAACGAAGt                                                                                                                                >  1:6263305/1‑150 (MQ=255)
                       gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGAAAATAGCGATTTAAAGCACTAAGt                                                                                                                                >  2:2507254/1‑150 (MQ=255)
                        tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAGATATAAAGCACaaaaat                                                                                                                               >  1:3480623/1‑147 (MQ=255)
                          aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAAGAAAATAGCGATGTAAATCGCAAAGtttt                                                                                                                             >  1:9365921/1‑149 (MQ=255)
                            ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                                           <  1:72074/150‑1 (MQ=255)
                             tttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTGAAAGAAATAAATAAAATATCGATGTAAATCACTAATTTTatat                                                                                                                          >  1:9088200/1‑150 (MQ=255)
                              ttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAAtt                                                                                                                         >  2:10190145/1‑150 (MQ=255)
                               tCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTg                                                                                                                        <  1:3664114/150‑1 (MQ=255)
                                cGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgc                                                                                                                       <  1:4748166/150‑1 (MQ=255)
                                cGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACAAAATCGATGTAAATCACAAAGTTTaaaattc                                                                                                                       >  2:1501840/1‑146 (MQ=255)
                                  gggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAGAGCACTAAGTTTAAATTgcgc                                                                                                                     <  2:665761/150‑1 (MQ=255)
                                   ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACGAATTTTATATTgcgcg                                                                                                                    >  2:11567879/1‑150 (MQ=255)
                                    gAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcgc                                                                                                                   <  1:2152804/150‑1 (MQ=255)
                                    gAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcgc                                                                                                                   <  2:1944942/150‑1 (MQ=255)
                                     aaGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGcc                                                                                                                  <  2:3680466/150‑1 (MQ=255)
                                       ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAATAAAAAAAGACAAAAACCAAGTAAAACAAAAAGTTTAAAATTAGCGccca                                                                                                                >  1:12016165/1‑148 (MQ=255)
                                        gATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAAt                                                                                                               <  1:15194332/150‑1 (MQ=255)
                                          tataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCatgat                                                                                                             <  2:5558757/150‑5 (MQ=255)
                                          tataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTCAAAGAAATAAAGACAAAAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCa                                                                                                             >  2:4652381/1‑150 (MQ=255)
                                            taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg                                                                                                           <  2:9381434/150‑1 (MQ=255)
                                             agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATAAAGACAAAATCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATAATgg                                                                                                          >  2:218869/1‑150 (MQ=255)
                                             agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAAAAAAAAAGAAAAAAACGATTTAAAGCACAAAATTTAAATTTAGCGCCacacctgg                                                                                                          >  1:15483693/1‑143 (MQ=255)
                                                   ttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCATGATTGGCGCGca                                                                                                    <  2:13068860/150‑1 (MQ=255)
                                                       gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAg                                                                                                <  2:12238803/150‑1 (MQ=255)
                                                       gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAg                                                                                                <  2:11162163/150‑1 (MQ=255)
                                                         aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTTTAAAAAAAATAAAAAAAAAAAAAAAAAAAAAGATGTAAATAAAAAAATTTAAAATTATGGCCAATCAAGGCCCGCACAAGca                                                                                              >  1:4536537/1‑149 (MQ=255)
                                                         aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAAAAATTAAAAAAAAAAAAAAAAAAATAGAAGTAAAGCAAAAAATTTAAATTTAGCGCCCATACAGGAGCGCACaaaca                                                                                              >  1:6008511/1‑147 (MQ=255)
                                                         aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAAAAAATAAAATAAAAAAAAAAAAAATCCATGTAAATAACAAACTTTTAAATTAGCGCCAAACAATGCGCGCCCaacca                                                                                              >  1:3127177/1‑147 (MQ=255)
                                                          agcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCta                                                                                             <  2:7312446/150‑1 (MQ=255)
                                                           gcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                                           >  2:8175409/1‑119 (MQ=255)
                                                                     ggAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAAAAAAGACAATAGCGATGTTAAGCACAAATTTTAAATTTCGCGCCAATAAAGGCGCGCACAAGCGATAAAAACaaac                                                                                  >  2:14627681/1‑148 (MQ=255)
                                                                          gtttttttCGTTCTACATTTGCTTATACATAACATTCTTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACATTAGCTTTTTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAtt                                                                             <  1:10808807/147‑1 (MQ=255)
                                                                            aTTTTTCGTTCGACCTTTGCTTATACATAACAGTGTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAACACAATAGCTATGTAAAGAACTAATTTTAAATTGCGCGCCAATCTTGGCGCGCACAAGCTATAATACCAACCTAATTtc                                                                           <  1:7385167/150‑1 (MQ=255)
                                                                             tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAAAGCGATTTAAAGCCAGAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAATtct                                                                          >  2:6466652/1‑150 (MQ=255)
                                                                              ttttCGTTCGACCTTTGCTTATACATAACAGTCGCTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                                         >  2:3553247/1‑150 (MQ=255)
                                                                                        aCCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACAAATTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCAAATTTATCCAACTCTTag                                                               >  2:12718988/1‑150 (MQ=255)
                                                                                         ccTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAATTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTaga                                                              <  1:15345918/150‑1 (MQ=255)
                                                                                          cTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAAGACAAAAGCGATGTAAAACACTAAGTTTAAATTGAGCGCCAAACATGGCGCGCACAAGCGATAAAACCAAACAAAATTATCCTCCACTTagag                                                             >  2:280617/1‑150 (MQ=255)
                                                                                           tttGCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGt                                                            <  2:3146989/150‑1 (MQ=255)
                                                                                             tGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGa                                                          <  2:4249205/150‑1 (MQ=255)
                                                                                               cTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAAACAAGACAAAAGCGAAGTAAAGCACGAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAACTATAAAACCCACCAAAATTATACTCCGCTTAGAGTGACt                                                        >  2:10643283/1‑150 (MQ=255)
                                                                                                ttATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCGTAGAGTGACta                                                       >  2:10453237/1‑150 (MQ=255)
                                                                                                    aCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCAATAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtc                                                   <  2:459017/150‑1 (MQ=255)
                                                                                                      aTAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAAACAAGACAAAAGCGATGTAAATCACTAAGTTTAAATTTCGCGCCAAACATGGCGCGCCCAAGCGATAATAACAACCAAATTTATCCACCTCTTAGAGTTAATATAtcta                                                 >  2:6089876/1‑149 (MQ=255)
                                                                                                          cAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGa                                             <  2:4474249/150‑1 (MQ=255)
                                                                                                            gTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATa                                           <  1:1038645/150‑1 (MQ=255)
                                                                                                               gTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGaa                                        <  2:1733631/150‑1 (MQ=255)
                                                                                                               gTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGaa                                        >  1:13993832/1‑150 (MQ=255)
                                                                                                                tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                                       >  1:2269273/1‑150 (MQ=255)
                                                                                                                  tttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTg                                     <  2:7901832/150‑1 (MQ=255)
                                                                                                                  tttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTg                                     <  2:3874804/150‑1 (MQ=255)
                                                                                                                   atatAATTTATAAAATAATTCAAAGAAAACAAGACAATAGCGAAGAACACCACTACGTATAAATTGCGCGACACTCCTGCCGCCCACAAGCTATAATACCAACCAAATTACTCCACCTCTTACAGTGACTATATCTCCTCATAGAATTGc                                    >  1:10014881/4‑150 (MQ=255)
                                                                                                                    tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                                   <  2:6958494/150‑1 (MQ=255)
                                                                                                                      tAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGt                                 <  1:8180029/150‑1 (MQ=255)
                                                                                                                        aTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAt                               >  2:11389453/1‑150 (MQ=255)
                                                                                                                         tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAtt                              <  2:4742525/150‑1 (MQ=255)
                                                                                                                         attATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAtt                              <  2:9752935/149‑1 (MQ=255)
                                                                                                                           tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                            >  1:7617545/1‑150 (MQ=255)
                                                                                                                           tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                            >  2:5240569/1‑150 (MQ=255)
                                                                                                                             taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt                          <  2:5859400/150‑1 (MQ=255)
                                                                                                                             taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt                          >  2:2273817/1‑150 (MQ=255)
                                                                                                                               aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTg                                     >  1:8614349/1‑137 (MQ=255)
                                                                                                                                aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc                       >  2:13418134/1‑150 (MQ=255)
                                                                                                                                  taatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaa                     <  2:8602269/150‑1 (MQ=255)
                                                                                                                                     ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg                  >  1:1234296/1‑150 (MQ=255)
                                                                                                                                     ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg                  >  1:9839106/1‑150 (MQ=255)
                                                                                                                                      tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc                 <  2:12519788/150‑1 (MQ=255)
                                                                                                                                      tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc                 >  1:1144641/1‑150 (MQ=255)
                                                                                                                                      tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc                 >  1:1115665/1‑150 (MQ=255)
                                                                                                                                        aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGTATTGCGGTATTGTCTATCAAATGccc               >  2:11714291/1‑150 (MQ=255)
                                                                                                                                        aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGACTTGCGGTATTGACTATCAAATGccc               >  1:8891507/1‑150 (MQ=255)
                                                                                                                                        aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc               <  2:1084929/150‑1 (MQ=255)
                                                                                                                                         aaGAAATCAAGACAATAGTGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt              <  1:13793377/150‑1 (MQ=255)
                                                                                                                                         aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAAGTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt              <  1:14326594/150‑1 (MQ=255)
                                                                                                                                           gAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGa            <  2:8282750/150‑1 (MQ=255)
                                                                                                                                               tCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCg        >  1:5291319/1‑150 (MQ=255)
                                                                                                                                               tCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATATCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCg        >  1:7118332/1‑150 (MQ=255)
                                                                                                                                                 taGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt      <  1:15051498/149‑1 (MQ=255)
                                                                                                                                                 aaGACAATAGCGATGTAAAGCACTAAGTTTACATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt      <  1:14664632/150‑1 (MQ=255)
                                                                                                                                                 aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt      <  1:2886301/150‑1 (MQ=255)
                                                                                                                                                 aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt      <  1:2799088/150‑1 (MQ=255)
                                                                                                                                                 aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCAGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt      <  2:14413311/150‑1 (MQ=255)
                                                                                                                                                    aCAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgttgtt   >  1:12679546/1‑150 (MQ=255)
                                                                                                                                                    aCAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgttgtt   <  2:5000714/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTT  >  NC_000913/2470292‑2470590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: