Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,639,262 T→G 15.1% N8H (AAC→CAC)  yfgM ← ancillary SecYEG translocon subunit; putative anti‑RcsB factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,639,2620TG15.1% 2198.9 / 137.8 832N8H (AAC→CAC) yfgMancillary SecYEG translocon subunit; putative anti‑RcsB factor
Reads supporting (aligned to +/- strand):  ref base T (162/528);  new base G (139/3);  total (301/531)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 854 total aligned reads displayed.

ATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTC  >  NC_000913/2639113‑2639411
                                                                                                                                                     |                                                                                                                                                     
aTAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGtt                                                                                                                                                       >  2:8904891/1‑150 (MQ=255)
 tAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGttt                                                                                                                                                      <  2:12521542/150‑1 (MQ=255)
   ggCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTc                                                                                                                                                    <  1:10516549/150‑1 (MQ=255)
    gCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGttttaa                                                                                                                                                   >  2:8491766/1‑148 (MQ=255)
    gCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCg                                                                                                                                                   >  2:14836487/1‑150 (MQ=255)
       agagAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTtc                                                                                                                                                >  1:14969561/1‑150 (MQ=255)
       agagAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtc                                                                                                                                                <  1:8352220/150‑1 (MQ=255)
       agagAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtc                                                                                                                                                <  1:2899961/150‑1 (MQ=255)
       agagAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtc                                                                                                                                                <  2:5488740/150‑1 (MQ=255)
        gagaAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGTGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtct                                                                                                                                               <  2:14544298/150‑1 (MQ=255)
        gagaAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtct                                                                                                                                               <  2:12403859/150‑1 (MQ=255)
        gagaAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAAACGCTTCTACCTGGTCGTTTTTGttgt                                                                                                                                               >  1:2002300/1‑148 (MQ=255)
         agaAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtctc                                                                                                                                              <  2:1093347/150‑1 (MQ=255)
         agaAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtctc                                                                                                                                              <  2:2275301/150‑1 (MQ=255)
         agaAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtctc                                                                                                                                              <  1:11586825/150‑1 (MQ=255)
             gCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTaa                                                                                                                                          <  1:8237594/150‑1 (MQ=255)
              cGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTaaa                                                                                                                                         <  1:10794730/150‑1 (MQ=255)
                  gCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGGTCTCGTAAATTTc                                                                                                                                     >  1:9062881/1‑150 (MQ=255)
                   cGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTcc                                                                                                                                    <  1:11177102/150‑1 (MQ=255)
                    gTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTAGTTCTCGGAAATTTACa                                                                                                                                   >  2:5260258/1‑150 (MQ=255)
                         gAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTCTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTg                                                                                                                              <  1:14509932/150‑1 (MQ=255)
                          aaTCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTGTCGTAAATTTAAACGCTGt                                                                                                                             >  2:4125161/1‑150 (MQ=255)
                            tCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGtcc                                                                                                                           <  2:2814222/150‑1 (MQ=255)
                                ccTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGtccttct                                                                                                                       <  1:14274877/150‑1 (MQ=255)
                                ccTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGtccttct                                                                                                                       <  2:6741363/150‑1 (MQ=255)
                                 cTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGtccttctc                                                                                                                      <  2:7322312/150‑1 (MQ=255)
                                 cTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGtccttctc                                                                                                                      <  1:10972523/150‑1 (MQ=255)
                                 cTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTGTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGTTCACCGAAATTTCCCCGCGGtccaacac                                                                                                                      >  2:11484522/1‑145 (MQ=255)
                                  tGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGGTCGGTCTCGGAAAGTTCCCCCCGGtccctctcc                                                                                                                     >  2:3140347/1‑150 (MQ=255)
                                   gATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGtccttctcca                                                                                                                    >  1:12639337/1‑149 (MQ=255)
                                      ggCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCtccttcc                                                                                                                 <  2:3776405/150‑1 (MQ=255)
                                      ggCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCtccttcc                                                                                                                 >  2:8735387/1‑150 (MQ=255)
                                       gCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTGTCGTAAATTTCCACGCAGTCCTTCtccttcca                                                                                                                >  2:4260410/1‑149 (MQ=255)
                                             ccAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACACCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTGTCGTGAAATTCCCCGCGGTGCTACTCCGGCCATAAcac                                                                                                          >  1:12253047/1‑148 (MQ=255)
                                              cAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCa                                                                                                         <  2:3359152/150‑1 (MQ=255)
                                              cAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCa                                                                                                         <  1:3353673/150‑1 (MQ=255)
                                                gTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTACACGCTGGACTTCTACTTCCTTAACCCAGt                                                                                                       >  2:771583/1‑150 (MQ=255)
                                                gTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTAGTTCTCGTAAATTTCCACGCTTTACTTCTCCTTCCTTAAACCAGt                                                                                                       >  2:5013668/1‑150 (MQ=255)
                                                gTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTCTCGGAAAATTACAAGCTGTACGTCTCCTTCCTTAAccccct                                                                                                       >  1:7926409/1‑147 (MQ=255)
                                                 tAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTCTCGTAAATTTCCACGCTGTCCTTCTCCGTCCTTAAACCAGTa                                                                                                      >  1:7648728/1‑150 (MQ=255)
                                                   gcgcCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTCGCGGAAATTTACACGCGGTCCGACACCATCCATAAACCacaaac                                                                                                    >  2:11822782/1‑145 (MQ=255)
                                                   gcgcCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTGGGGGAAATTTACACGCGGTCCGTCGACATCCGTAAACCAGTTAc                                                                                                    >  2:10506534/1‑150 (MQ=255)
                                                    cgcCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTACTTAACCCAGTAACg                                                                                                   >  2:12821253/1‑150 (MQ=255)
                                                      ccagccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTg                                                                                                 <  1:399125/150‑1 (MQ=255)
                                                      ccagccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCATCCTTAACCCAGTAACCTg                                                                                                 >  2:11241551/1‑150 (MQ=255)
                                                      ccagccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTGTCGTAAAATTAAACCCGGTGCTTCTCAGTCCGTAACCCAGTAACCTg                                                                                                 >  1:1657766/1‑150 (MQ=255)
                                                         gccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGTTGTCGTAAAATTACACCCGGTCCATCTCCATACGTAACACAGTAAAGTgcgc                                                                                              >  2:9371051/1‑150 (MQ=255)
                                                         gccaATCAGTGCGCCAACACCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGTCGTAAATTTACAAGCAGTACATATACTTCCATAACCCACTAAACTgcgc                                                                                              >  1:6186932/1‑150 (MQ=255)
                                                             aTCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTCCTACCTGGTCGTGTTCGTTGTGGTAAATTTAAACGCGGTGCATATACTTACTTAAACCACTAAAGTGCGCAAAt                                                                                          >  2:9747919/1‑150 (MQ=255)
                                                                aGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATgcg                                                                                       <  2:691029/150‑1 (MQ=255)
                                                                      ccAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGGTCTCGTAAATTTCCACGCTGTCCGTCTCCGTCCATAACCCAGTAACGTGCGCAAATGCGCGGCTa                                                                                 >  2:3652898/1‑150 (MQ=255)
                                                                         aCGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGc                                                                              <  1:5707712/150‑1 (MQ=255)
                                                                          cGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGGTGGTTGTCGTAAATTTACACCCAGTGCATATACCTCCTTAAACCACTAACCAGCGCCAAAGCGCCGCGAcccg                                                                             >  1:4338697/1‑147 (MQ=255)
                                                                            cccAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTAGTTCTCGTAAATTTCCACCCGTTCCGTGTCCTTCCATAAACCACTAACCTGCGCAAAATCGCGGCTACGCAAt                                                                           >  2:806603/1‑150 (MQ=255)
                                                                             ccAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATc                                                                          <  1:13724041/150‑1 (MQ=255)
                                                                                 aaTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTgc                                                                      <  1:9799363/150‑1 (MQ=255)
                                                                                 aaTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTgc                                                                      <  2:4403826/150‑1 (MQ=255)
                                                                                 aaTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTgc                                                                      <  1:2199775/150‑1 (MQ=255)
                                                                                   tCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTgcgc                                                                    <  1:6931266/150‑1 (MQ=255)
                                                                                           aCAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCg                                                            <  1:8832495/150‑1 (MQ=255)
                                                                                            cAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGt                                                           <  2:7479274/150‑1 (MQ=255)
                                                                                            cAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGt                                                           <  1:6020677/150‑1 (MQ=255)
                                                                                            cAGACAGTGCTTTGCCATTGTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGt                                                           <  2:10071052/150‑1 (MQ=255)
                                                                                             aGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGtt                                                          <  1:7181401/150‑1 (MQ=255)
                                                                                              gCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTATCGTAAAATTACACGCTGTCCATATACTTCCTTAACCCACTAAAGTGCGCAAATGCGCGGCTACGCGATCCAGCGCAACAGcccgtt                                                         >  2:8312224/1‑146 (MQ=255)
                                                                                               ccAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTg                                                        <  1:14020553/150‑1 (MQ=255)
                                                                                                cAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAAATTACACGCTGTCCTTCTACTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCAAACCTGCGCAACTGCCGgtttc                                                       >  2:15400316/1‑148 (MQ=255)
                                                                                                   tGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCa                                                    <  1:262008/150‑1 (MQ=255)
                                                                                                     cTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTAGTTCTCGTAAAATTCCACGCTGTCCGTATCCTTCCTGAACCCACTAACGTGCGCAAAAGCGCGGCTACGCTATACTGCGCAACAGCCCTTTGCTCAcc                                                  >  2:13393694/1‑150 (MQ=255)
                                                                                                         gCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCTCCagag                                              <  1:3585336/150‑1 (MQ=255)
                                                                                                          ccATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTGTCGGAAATTTACACCCAGGGCTTCTCCGTCCGTAAACCACGAACGTGCGCCAATGCGCCGCGACGCGATGCGGCCCAACAGCCCGTTTCTCACCACAgc                                             >  2:158826/1‑150 (MQ=255)
                                                                                                           cATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAgcg                                            <  2:14100610/150‑1 (MQ=255)
                                                                                                              tttCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTCTCGTAAAATTACCCCCGGTGCATATCCGTCCGAAAAACACTAAAGTTCGCAAAAGCGCGGCGAAGCTAACAAGCGCAACTGCCGGTTGCTCACCACAGCGCaa                                         >  2:101631/1‑150 (MQ=255)
                                                                                                               ttCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCaaa                                        <  1:13199003/150‑1 (MQ=255)
                                                                                                                  aGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTGTCGGAAATTTACACCCGGTCCTTCACCGTCCTTAACCCACTAACCTTCGCAAATTCGCCGCGACGCGATACAGCCCAACTGGCGGTTGCGAACCAGAGCGCAAATcc                                     >  2:4756824/1‑150 (MQ=255)
                                                                                                                  aGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATcc                                     <  1:11965818/150‑1 (MQ=255)
                                                                                                                    cAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCtt                                   <  1:5353116/150‑1 (MQ=255)
                                                                                                                     aaaaaaGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTc                                  <  2:13805409/150‑1 (MQ=255)
                                                                                                                      aaaaaGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCa                                 <  1:10260128/150‑1 (MQ=255)
                                                                                                                        aaaGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAAAGTGCGCAAATGCGCGGCTAAGCTATACTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCact                               >  2:2575415/1‑150 (MQ=255)
                                                                                                                         aaGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAACTGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCacta                              <  2:6236179/150‑1 (MQ=255)
                                                                                                                              tttAACCGCTTCTACCTGGTCGTTTTAGTTCTCGTAAATTTACACCCGTTCCGTCTCCGTCCTTAACCCCGGAACCGGCGCAAATGCGCGGCGACCCGAACCTGCGCAACAGCCCGTTGGTCACCCGAGCGCCAAACCATCACAAcagcg                         >  2:5841200/1‑149 (MQ=255)
                                                                                                                                   ccGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGcc                    <  1:7446917/150‑1 (MQ=255)
                                                                                                                                   ccGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGcc                    <  2:6381095/150‑1 (MQ=255)
                                                                                                                                     gCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGt                  <  2:9074332/150‑1 (MQ=255)
                                                                                                                                       ttCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTa                <  1:9569523/150‑1 (MQ=255)
                                                                                                                                       ttCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTa                <  1:6518961/150‑1 (MQ=255)
                                                                                                                                        tCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAg               <  2:12950122/150‑1 (MQ=255)
                                                                                                                                         cTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGc              <  1:15500706/150‑1 (MQ=255)
                                                                                                                                         cTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGc              <  2:5975221/150‑1 (MQ=255)
                                                                                                                                         cTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGc              <  2:2002300/150‑1 (MQ=255)
                                                                                                                                            ccTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCATTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCAc           <  1:10028384/150‑1 (MQ=255)
                                                                                                                                              tGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACtt         <  1:15192267/150‑1 (MQ=255)
                                                                                                                                                gTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCa       <  1:3162992/150‑1 (MQ=255)
                                                                                                                                                gTCGTTTTAGTTCTCGTAAAATTACACGCAGTCCTTCTCCTTCCTTAACCCAGTAAACTTCGCAAATTCGCGGCTACGCTATCCAGCGCAACAGCCGTTTTCTCACCAGAGCGCAAATACATAACAACATCTGTTCCGTTAGCCACATCa       >  2:12189439/1‑150 (MQ=255)
                                                                                                                                                  cGTTTTCGTTCTCGTATATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGa     <  1:4768998/150‑1 (MQ=255)
                                                                                                                                                  cGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGa     <  1:5077101/150‑1 (MQ=255)
                                                                                                                                                  cGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGa     <  2:15089649/150‑1 (MQ=255)
                                                                                                                                                  cGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGa     <  1:9281519/150‑1 (MQ=255)
                                                                                                                                                     tttCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTc  >  2:9606599/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTC  >  NC_000913/2639113‑2639411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: