Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,639,269 C→G 11.0% E5D (GAG→GAC yfgM ← ancillary SecYEG translocon subunit; putative anti‑RcsB factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,639,2690CG11.0% 2433.7 / 59.2 787E5D (GAG→GACyfgMancillary SecYEG translocon subunit; putative anti‑RcsB factor
Reads supporting (aligned to +/- strand):  ref base C (184/494);  new base G (94/2);  total (290/497)
Fisher's exact test for biased strand distribution p-value = 2.38e-44
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 824 total aligned reads displayed.

AGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGC  >  NC_000913/2639120‑2639418
                                                                                                                                                     |                                                                                                                                                      
agagAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtc                                                                                                                                                        <  2:5488740/150‑1 (MQ=255)
  agaAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtctc                                                                                                                                                      <  2:1093347/150‑1 (MQ=255)
  agaAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtctc                                                                                                                                                      <  1:11586825/150‑1 (MQ=255)
   gaAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCg                                                                                                                                                     >  1:7296348/1‑150 (MQ=255)
    aaGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGTCGt                                                                                                                                                    >  2:14554679/1‑150 (MQ=255)
     aGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTa                                                                                                                                                   >  1:2067824/1‑150 (MQ=255)
     aGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTa                                                                                                                                                   >  2:3915683/1‑150 (MQ=255)
      gCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGTTCTCGTaa                                                                                                                                                  >  1:13793569/1‑150 (MQ=255)
       cGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTaaa                                                                                                                                                 >  2:10455721/1‑150 (MQ=255)
         gAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGGTCTCGTAAAtt                                                                                                                                               >  1:12485687/1‑150 (MQ=255)
          aGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAAttt                                                                                                                                              <  1:12822013/150‑1 (MQ=255)
           gCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTc                                                                                                                                             <  1:14958061/150‑1 (MQ=255)
            cGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTcc                                                                                                                                            <  2:6539877/150‑1 (MQ=255)
             gTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCa                                                                                                                                           <  2:6355551/150‑1 (MQ=255)
             gTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACACCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGATTTCGTTCTCGTAAATTTCCa                                                                                                                                           <  2:1512006/150‑1 (MQ=255)
              tGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAATATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTTGTTGTGGTTAAttttaac                                                                                                                                          >  1:6718409/1‑146 (MQ=255)
              tGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGGTCTCGTAAATTTacac                                                                                                                                          >  1:321146/1‑150 (MQ=255)
              tGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCAc                                                                                                                                          <  1:4817555/150‑1 (MQ=255)
              tGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTGTAGTAAAATTacac                                                                                                                                          >  1:4594946/1‑150 (MQ=255)
               gCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACg                                                                                                                                         <  2:839296/150‑1 (MQ=255)
               gCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACg                                                                                                                                         <  1:5173373/150‑1 (MQ=255)
                cAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGGTGTCGGAAAATTACACGc                                                                                                                                        >  1:5370293/1‑150 (MQ=255)
                  gAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTg                                                                                                                                      <  2:7427490/150‑1 (MQ=255)
                  gAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCCGGTCGTTTTCGTTCTCGTAAATTTCCACGCTg                                                                                                                                      <  1:8092355/150‑1 (MQ=255)
                     tCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGtcc                                                                                                                                   <  1:6436008/150‑1 (MQ=255)
                       aaCCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGtcctt                                                                                                                                 <  2:3173990/150‑1 (MQ=255)
                       aaCCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGtcctt                                                                                                                                 <  2:4426208/150‑1 (MQ=255)
                       aaCCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGGCGTAAATTTCCACGCGGtccgt                                                                                                                                 >  2:1540735/1‑148 (MQ=255)
                         ccTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGtccttct                                                                                                                               <  1:9245641/150‑1 (MQ=255)
                          cTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGtccttctc                                                                                                                              <  1:10972523/150‑1 (MQ=255)
                           tGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGtccttctcc                                                                                                                             <  2:9484189/150‑1 (MQ=255)
                              tGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGtccttctccttc                                                                                                                          <  2:9612148/150‑1 (MQ=255)
                               ggCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTACAAGCAGTCCTTAtccttcc                                                                                                                         >  2:8858148/1‑150 (MQ=255)
                                  tGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGTCGTAAATTTTCAACCTGTGCTTATCCATCCGTa                                                                                                                      >  2:524026/1‑150 (MQ=255)
                                     tCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAAcc                                                                                                                   <  2:12489114/150‑1 (MQ=255)
                                      ccAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTAGTTGTCGTAAATTTACACGCGGGGCTTCTCCATCCATAAacc                                                                                                                  >  1:2704154/1‑150 (MQ=255)
                                         gTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTAGTTCTCGTAAATTTCCACGCTTTACTTCTCCTTCCTTAAACCAGt                                                                                                               >  2:5013668/1‑150 (MQ=255)
                                            gcgcCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTAGTTCTAGTAAAATTGCACGCGGTGCGTATCAGTCCATAAACCCGTAAc                                                                                                            >  1:11190330/1‑150 (MQ=255)
                                             cgcCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTAGTAAATTTCCACGCTGTCCTTCTCCATCCTTAACCCAGTAACg                                                                                                           >  2:12820294/1‑150 (MQ=255)
                                                   ccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAAACCAGTAACGTGCGCa                                                                                                     >  2:10039211/1‑150 (MQ=255)
                                                    caATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGATGTCCTTATACTTACTTAACCCAGTAAAGTTCGCaa                                                                                                    >  2:10160343/1‑150 (MQ=255)
                                                     aaTCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCaaa                                                                                                   <  2:6889814/150‑1 (MQ=255)
                                                      aTCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAAt                                                                                                  <  2:9754109/150‑1 (MQ=255)
                                                       tCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGTGGTAAATTTACAACCTGTGCATCTCCCTCCTTAAACCAGTAACGGGCGCAAAtt                                                                                                 >  2:2909639/1‑149 (MQ=255)
                                                          gTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATgcgc                                                                                              <  2:12047962/150‑1 (MQ=255)
                                                          gTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATgcgc                                                                                              <  1:7533435/150‑1 (MQ=255)
                                                          gTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATgcgc                                                                                              <  1:2248942/150‑1 (MQ=255)
                                                           tGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGcgcg                                                                                             <  1:14151189/150‑1 (MQ=255)
                                                                  aCGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGc                                                                                      <  1:7068771/150‑1 (MQ=255)
                                                                    gCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTa                                                                                    <  2:7649608/150‑1 (MQ=255)
                                                                      ccAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTCTCGGAAATTTCCACGCGGTCCTTCTCCTTCCTTAACCCACTAACGTGCGCAAATGCGCGGCTACGCTATc                                                                                  >  2:14205692/1‑150 (MQ=255)
                                                                          aaTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTgc                                                                              <  1:3410190/150‑1 (MQ=255)
                                                                               ccccAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAAc                                                                         <  1:1739757/150‑1 (MQ=255)
                                                                                 ccAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTg                                                                      <  2:4304877/150‑1 (MQ=255)
                                                                                  cAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGc                                                                     <  1:97926/150‑1 (MQ=255)
                                                                                     cAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGt                                                                  <  1:4054056/150‑1 (MQ=255)
                                                                                       gCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGttt                                                                <  2:5967139/150‑1 (MQ=255)
                                                                                       gCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGttt                                                                <  1:3641007/150‑1 (MQ=255)
                                                                                        ccAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGGTCTCGTAAATTTACAAGCGGTCCATCTCCATCCTTAACCCACTAAAGTGCGCAAAAGCGCGGCTACGCTATACTGCGCAACGGCCGTTTg                                                               >  2:12376308/1‑150 (MQ=255)
                                                                                          aGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGTTCTCGTAAATTTCCCCGCGGTCCGTCGCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCGACGCTATCCAGCGCAACAGCCCGTTGcg                                                             >  2:3727353/1‑149 (MQ=255)
                                                                                                 tGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCaga                                                      <  1:7851741/150‑1 (MQ=255)
                                                                                                 tGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCaga                                                      <  1:8735387/150‑1 (MQ=255)
                                                                                                  gCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCagag                                                     <  2:2309903/150‑1 (MQ=255)
                                                                                                  gCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCagag                                                     <  1:12090848/150‑1 (MQ=255)
                                                                                                    cATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAgcg                                                   <  2:3410218/150‑1 (MQ=255)
                                                                                                    cATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTACACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTAAACTATACTTCGCAACAGCCGTTTGCTCACCAGAgcg                                                   >  2:9161618/1‑150 (MQ=255)
                                                                                                     aTTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTCTCGTAAAATTCCACGCTGTCCTTATCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAgcgc                                                  >  2:14026537/1‑150 (MQ=255)
                                                                                                           aGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTGTCGGAAATTTACACCCGGTCCTTCACCGTCCTTAACCCACTAACCTTCGCAAATTCGCCGCGACGCGATACAGCCCAACTGGCGGTTGCGAACCAGAGCGCAAATcc                                            >  2:4756824/1‑150 (MQ=255)
                                                                                                           aGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATcc                                            <  2:5836250/150‑1 (MQ=255)
                                                                                                           aGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGTTCTCGTAAATTTCCACGCTGTCCATCTCCGTCCTTAAACCAGGAACCTGCGCAAAAGCGCGGCTACGCTATACAGCGCAACAGCCGTTTTCTCACCACAGCGCaaaaac                                            >  2:13709603/1‑147 (MQ=255)
                                                                                                             cAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCtt                                          <  2:1657766/150‑1 (MQ=255)
                                                                                                              aaaaaaGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTc                                         <  2:8688844/150‑1 (MQ=255)
                                                                                                               aaaaaGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCa                                        <  1:10260128/150‑1 (MQ=255)
                                                                                                               aaaaaGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTCTCGTAAATTTAAACGCTGTCCGTCACCGTACATAACCCACTAACCTGCGCAAAATCGCGGCTAAGCTATACATCGCAACATCCCGTTTAGCACCCCAGCGCAAAACCttga                                        >  2:4160415/1‑148 (MQ=255)
                                                                                                                         tAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCtgt                              <  1:1056281/150‑1 (MQ=255)
                                                                                                                         tAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCtgt                              <  1:13326396/150‑1 (MQ=255)
                                                                                                                            ccGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGcc                           <  2:11479300/150‑1 (MQ=255)
                                                                                                                             cGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCg                          <  1:5967794/150‑1 (MQ=255)
                                                                                                                              gCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGt                         <  1:8312224/150‑1 (MQ=255)
                                                                                                                                ttCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTa                       <  1:9569501/150‑1 (MQ=255)
                                                                                                                                  cTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGc                     <  2:2008646/150‑1 (MQ=255)
                                                                                                                                  cTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGc                     <  1:14503187/150‑1 (MQ=255)
                                                                                                                                  cTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGc                     <  1:15500706/150‑1 (MQ=255)
                                                                                                                                  cTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGc                     <  1:2977870/150‑1 (MQ=255)
                                                                                                                                     ccTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCATTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCAc                  <  1:10028384/150‑1 (MQ=255)
                                                                                                                                       tGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACtt                <  2:8226737/150‑1 (MQ=255)
                                                                                                                                       tGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACtt                <  1:15192088/150‑1 (MQ=255)
                                                                                                                                       tGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACtt                <  1:10804637/150‑1 (MQ=255)
                                                                                                                                         gTCGTTTTCGTTCTCGTAAATTTCCACGCAGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATTCGCGGCTACGCTATCCAGCGCAACTGCCGTTTGCTCACCAGAGAGCAAATACTTCACTACTGCTGTGCCGTTAGCCACATCa              >  2:12936138/1‑150 (MQ=255)
                                                                                                                                           cGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGa            <  2:6186932/150‑1 (MQ=255)
                                                                                                                                           cGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGa            <  2:7912690/150‑1 (MQ=255)
                                                                                                                                           cGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGa            <  2:11667604/150‑1 (MQ=255)
                                                                                                                                            gTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGAc           >  1:8986201/1‑150 (MQ=255)
                                                                                                                                                tCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCAc       <  2:11229451/150‑1 (MQ=255)
                                                                                                                                                 cGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCAcc      <  2:14969561/150‑1 (MQ=255)
                                                                                                                                                   ttCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCa    <  2:7153363/150‑1 (MQ=255)
                                                                                                                                                   ttCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCa    >  2:1328903/1‑150 (MQ=255)
                                                                                                                                                    tctcGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAg   <  1:6086964/150‑1 (MQ=255)
                                                                                                                                                    tctcGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAg   <  2:8986201/150‑1 (MQ=255)
                                                                                                                                                     ctcGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGc  <  2:2612772/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
AGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGC  >  NC_000913/2639120‑2639418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: