BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,965,131	G→T	11.1%	Q9K (CAA→AAA)	thyA ←	thymidylate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,965,131	0	G	T	11.1%	2249.8 / 53.7	706	Q9K (CAA→AAA)	thyA	thymidylate synthetase
Reads supporting (aligned to +/- strand): ref base G (196/409); new base T (98/3); total (294/412)
Fisher's exact test for biased strand distribution p-value = 3.51e-38
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 714 total aligned reads displayed.

TTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGG  >  NC_000913/2964982‑2965280

 tCAGGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTAGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGc                                                                                                                                                      <  2:12064450/150‑1 (MQ=255)

   aTGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCGTAGTGGAGCCCATTTTGCAt                                                                                                                                                    >  1:2586645/1‑150 (MQ=255)

     ggATGATGGAACGTAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCa                                                                                                                                                  >  2:5966996/1‑150 (MQ=255)

          atgGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCt                                                                                                                                             <  1:13645452/150‑1 (MQ=255)

           tgGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCGGTGTGCCGTCGTCGAGCACTTTTTGCATCAGTGCtc                                                                                                                                            >  2:15214189/1‑149 (MQ=255)

            ggAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTaa                                                                                                                                           <  2:7257987/150‑1 (MQ=255)

             gAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTaaa                                                                                                                                          <  1:5713841/150‑1 (MQ=255)

             gAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTaaa                                                                                                                                          <  2:7601486/150‑1 (MQ=255)

                 gCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTATGTGTGCCGTCGTCGAGCACGTTTTTCATAAGTTCTAAATaaa                                                                                                                                      >  1:14634678/1‑148 (MQ=255)

                  cAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTg                                                                                                                                     <  1:12630579/150‑1 (MQ=255)

                   aGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTATGTGTGCCATCGTCGAGCACATTTTTCATCAGTTATAAATaaagt                                                                                                                                    >  1:2330770/1‑146 (MQ=255)

                    ggTGGCAACGTTTAGTTGTCAACAGCGGGAATCGATCTTTCAGGTTAAAACGCATCTTATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGtt                                                                                                                                   <  1:14451579/150‑1 (MQ=255)

                       ggCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCa                                                                                                                                <  2:14157966/150‑1 (MQ=255)

                       ggCAACGTTTAGGTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCa                                                                                                                                <  1:1002894/150‑1 (MQ=255)

                        gCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCAt                                                                                                                               <  1:3270266/150‑1 (MQ=255)

                         cAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATg                                                                                                                              <  1:7681388/150‑1 (MQ=255)

                         cAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATg                                                                                                                              <  2:466317/150‑1 (MQ=255)

                          aaCGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTGTTTGTGTGTGGCGTCGTCGAGCACATTTTGCATAAGTTCTTAATAAAGTTTAAttg                                                                                                                             >  1:3649380/1‑148 (MQ=255)

                             gTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTGTTTGTGTGTGCCTTCGGAGGGCACATTTTGCATAAGTTGTAAATACAGTTTAAAGGtta                                                                                                                          >  1:14926462/1‑149 (MQ=255)

                              tttAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTGGTTATTTTTTCCTTCGTCGAGCACGTTTTTCAACAGTTCTAAATAAATTTTAATGGTTcc                                                                                                                         >  1:8448656/1‑150 (MQ=255)

                               ttAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTGTTTATGTGTGCCGTCGTCGAGCCCGTTTTGCATCAGGTCGAAATAATGTTTCATGGGTccg                                                                                                                        >  2:15006498/1‑149 (MQ=255)

                                  gTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAg                                                                                                                     <  1:15150912/150‑1 (MQ=255)

                                  gTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAg                                                                                                                     <  1:7026102/150‑1 (MQ=255)

                                   ttGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAgg                                                                                                                    <  1:7279295/150‑1 (MQ=255)

                                   ttGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTGTTTGTGTGTGGCGTCGGCGAGCACGTTTTGCATCAGTTCTAAATACTGGTTCATGGGTCCTCaaa                                                                                                                    >  2:3235386/1‑148 (MQ=255)

                                     gTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTGTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACAGTTTCATGGTTCCACAGGaa                                                                                                                  >  2:10363353/1‑150 (MQ=255)

                                      tCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGaaa                                                                                                                 <  1:13403447/150‑1 (MQ=255)

                                      tCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGaaa                                                                                                                 <  1:13129586/150‑1 (MQ=255)

                                      tCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTATGTGTGCCTTCGTCGAGCACATTTTTCATCATTTCTAAATACAGTTTAATGGTTACTCAGGaaa                                                                                                                 >  2:13452493/1‑150 (MQ=255)

                                        aCCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTTTTTGCCTTCGTAGAGCACTTTTTTCAACACTTCTAAATAAAGTTTCAAGGTTACTCAGGaaaag                                                                                                               >  2:1728422/1‑148 (MQ=255)

                                        aCCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTGTTTGTGTGTGCCTTCGTCGAGCACATTTTTGATCAGTTCTAAATAAAGTTTAATGGGTCCTCAGGaaaag                                                                                                               >  1:9577776/1‑148 (MQ=255)

                                         ccAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACgt                                                                                                              <  1:8954965/150‑1 (MQ=255)

                                          cAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACgtg                                                                                                             <  2:7092637/150‑1 (MQ=255)

                                           aGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACgtgt                                                                                                            <  1:7930547/150‑1 (MQ=255)

                                           aGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTATGTGTTGCTTCGGGGAGCACATTTTTGATCAGTTATAAATAAAGTTTAATGGTTACTCAGGAAAAgtgt                                                                                                            >  1:12808491/1‑150 (MQ=255)

                                              gggAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTGTTTGTGTGTGCCGTAGGCGAGCAAATTTTTGATAAGTTCTAAATAAAGTTTGATGGTTGCGCACGTAACGTGTTgg                                                                                                         >  1:6484809/1‑149 (MQ=255)

                                                gAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACATTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCtg                                                                                                       >  2:8937370/1‑150 (MQ=255)

                                                 aaTCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCtgt                                                                                                      <  2:14793424/150‑1 (MQ=255)

                                                  aTCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCtgtg                                                                                                     <  1:12000552/150‑1 (MQ=255)

                                                  aTCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCtgtg                                                                                                     <  2:15555895/150‑1 (MQ=255)

                                                    ccATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTGTTTGTGTGTTCCTTCGTCGCGCACATTTTTGATGAGTTCTAAAAAAAGTTTAATTGTTGCTCAGGAAAAGTTTTGCTGTggg                                                                                                   >  1:11296793/1‑150 (MQ=255)

                                                     cATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTGTTCTGTGTGCCCTCGTCGAGCCCGTTTTGCAACCGTTCTCAATACTGGTTCATGGGTCCTCACGAAACCGGGTGCTGTGAGc                                                                                                  >  2:11258121/1‑150 (MQ=255)

                                                         ttGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCGTCGTCGAGCACTTTTTGCATCAGTTCTAAATAATGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGcg                                                                                              >  2:1012408/1‑150 (MQ=255)

                                                            cAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTGTTTGTTTTTGACTTAGTGGATCAAATTTTTGATAAGTTGTAAAAAAAGTTTGAATGTTGATCAGGTAAACTGTTTCTGTGTGGGGcgacc                                                                                           >  2:5443765/1‑149 (MQ=255)

                                                                     aCGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCa                                                                                  <  1:4612876/150‑1 (MQ=255)

                                                                       gCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTTCCTTCGTCGAGCACTTTTTTCAACAGTTCTAAAAAATTTTTAATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGa                                                                                >  2:8167002/1‑150 (MQ=255)

                                                                        cATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGAc                                                                               <  2:4154340/150‑1 (MQ=255)

                                                                        cATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGAc                                                                               <  1:2924268/150‑1 (MQ=255)

                                                                        cATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGAc                                                                               <  2:5031823/150‑1 (MQ=255)

                                                                              atgaCCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTAGTCGAGCACGTTTTTCATCAGTTCTAAATAAAGTTTGATGGTTCCTCAGGAAACGTGTTGCGGTGGGGTGCGACGATATTCCCAGACcatcaa                                                                         >  2:1897429/1‑149 (MQ=255)

                                                                               tgaCCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATg                                                                        <  2:1088312/150‑1 (MQ=255)

                                                                                 aCCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGAt                                                                      <  1:6056818/150‑1 (MQ=255)

                                                                                 aCCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGAt                                                                      <  1:4325357/150‑1 (MQ=255)

                                                                                 aCCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGAt                                                                      >  2:2271036/1‑150 (MQ=255)

                                                                                     aaaaTGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATcaca                                                                  <  1:1661326/150‑1 (MQ=255)

                                                                                        aTGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACAccc                                                               <  1:86962/150‑1 (MQ=255)

                                                                                         tGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCcg                                                              <  1:9702325/150‑1 (MQ=255)

                                                                                         tGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCcg                                                              <  1:6725663/150‑1 (MQ=255)

                                                                                              aGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACAGTTTCAATGTTCCTCAGGAAACGTGTTTCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACa                                                         >  2:8753156/1‑150 (MQ=255)

                                                                                                 gttccggttccgGTACGGTCGTTTTTGTGTGTGCCGTCGTCGCGCACGTTTTTGAACAGGTGTAAATAATGTTTAATGGTTTCTCAGGTAAAGTGGTTGTGTGGGGTGCGTCGATATTCCCACAACAAAATGATAACAACCCCGAccaac                                                      >  2:13097191/1‑146 (MQ=255)

                                                                                                   tccggttccggtACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAatcat                                                    <  2:13607334/150‑1 (MQ=255)

                                                                                                    ccggttccggtACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAatcatc                                                   <  2:11658905/150‑1 (MQ=255)

                                                                                                        ttccggtACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGa                                               <  2:852811/150‑1 (MQ=255)

                                                                                                            ggtACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGa                                           <  2:2487331/150‑1 (MQ=255)

                                                                                                                 ggTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGaa                                      <  2:12326611/150‑1 (MQ=255)

                                                                                                                  gTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAAt                                     >  2:15473800/1‑150 (MQ=255)

                                                                                                                     gTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTg                                  >  2:5652969/1‑150 (MQ=255)

                                                                                                                      tttttCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGc                                 >  1:12215844/1‑150 (MQ=255)

                                                                                                                      tttttCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGc                                 <  2:2116198/150‑1 (MQ=255)

                                                                                                                      tttttCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGc                                 <  2:6380853/150‑1 (MQ=255)

                                                                                                                        tttCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGccc                               <  1:6907192/150‑1 (MQ=255)

                                                                                                                          tCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCa                             <  1:10946140/150‑1 (MQ=255)

                                                                                                                             gtgtgCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGc                          <  1:1897429/150‑1 (MQ=255)

                                                                                                                                tgCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGa                       <  1:9345570/150‑1 (MQ=255)

                                                                                                                                tgCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGa                       <  1:5467052/150‑1 (MQ=255)

                                                                                                                                 gCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGAt                      >  2:6633885/1‑150 (MQ=255)

                                                                                                                                    ttCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACTATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTa                   >  2:3646970/1‑150 (MQ=255)

                                                                                                                                     tcgtcgAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTAc                  <  2:14538057/150‑1 (MQ=255)

                                                                                                                                     tcgtcgAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTAc                  <  2:7782918/150‑1 (MQ=255)

                                                                                                                                     tcgtcgAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTAc                  >  2:4459446/1‑150 (MQ=255)

                                                                                                                                     tcgtcgAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTAc                  >  2:12810837/1‑150 (MQ=255)

                                                                                                                                      cgtcgAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACt                 <  1:3936049/150‑1 (MQ=255)

                                                                                                                                      cgtcgAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACt                 <  2:13920071/150‑1 (MQ=255)

                                                                                                                                          gAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCAc             >  1:11687071/1‑150 (MQ=255)

                                                                                                                                          gAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCAc             >  2:8103566/1‑150 (MQ=255)

                                                                                                                                            gCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCAccc           <  1:3460592/150‑1 (MQ=255)

                                                                                                                                            gCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCAccc           <  1:15473800/150‑1 (MQ=255)

                                                                                                                                             cACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCa          <  2:12902145/150‑1 (MQ=255)

                                                                                                                                             cACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCa          <  1:8226633/150‑1 (MQ=255)

                                                                                                                                              aCTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAg         <  1:4945369/150‑1 (MQ=255)

                                                                                                                                              aCTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAg         <  1:5240557/150‑1 (MQ=255)

                                                                                                                                              aCTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAg         >  2:9170272/1‑150 (MQ=255)

                                                                                                                                               cTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAgg        <  2:3111613/150‑1 (MQ=255)

                                                                                                                                                tttttGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGc       >  1:10369640/1‑150 (MQ=255)

                                                                                                                                                tttttGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGc       >  2:2803153/1‑150 (MQ=255)

                                                                                                                                                  tttGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCAc     <  2:688341/150‑1 (MQ=255)

                                                                                                                                                   ttGCATCAGTTCTATATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCAcc    <  1:1398921/150‑1 (MQ=255)

                                                                                                                                                   ttGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCAcc    <  2:9539008/150‑1 (MQ=255)

                                                                                                                                                     gCATCAGTTCTAACTACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCgg  <  2:12509316/150‑1 (MQ=255)

                                                                                                                                                     gCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCgg  <  2:13770745/150‑1 (MQ=255)

TTCATGGATGATGGAACGCAGGTGGCAACGTTTAGTTGTCACCAGCGGGAATCCATCTTGCAGGTTAAAACGCATCTGATGACCAAAAATGGAAAGCGTTCCGGTTCCGGTACGGTCGTTTTTCTGTGTGCCTTCGTCGAGCACTTTTTGCATCAGTTCTAAATACTGTTTCATGGTTCCTCAGGAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGG  >  NC_000913/2964982‑2965280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`