BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,054,905	T→G	8.9%	Q180H (CAA→CAC)	ygfB ←	UPF0149 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,054,905	0	T	G	8.9%	2069.0 / 43.0	717	Q180H (CAA→CAC)	ygfB	UPF0149 family protein
Reads supporting (aligned to +/- strand): ref base T (181/455); new base G (79/2); total (260/457)
Fisher's exact test for biased strand distribution p-value = 3.98e-36
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 756 total aligned reads displayed.

AAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACC  >  NC_000913/3054756‑3055054

 aaTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGtt                                                                                                                                                      >  2:10361430/1‑150 (MQ=255)

  aTCAGCGCGGCGCGGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTCCGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTg                                                                                                                                                     <  2:4655542/150‑1 (MQ=255)

   tCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGc                                                                                                                                                    <  1:9593105/150‑1 (MQ=255)

   tCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGc                                                                                                                                                    <  1:1184502/150‑1 (MQ=255)

     aGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTTCTTGGGGGGCGGGGGGGTGCgg                                                                                                                                                  >  1:5802201/1‑150 (MQ=255)

      gcgcGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGa                                                                                                                                                 <  1:9655366/150‑1 (MQ=255)

       cgcgGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGAt                                                                                                                                                <  1:12615340/150‑1 (MQ=255)

         cggcgCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGa                                                                                                                                              <  2:8121003/150‑1 (MQ=255)

         cggcgCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCGTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGa                                                                                                                                              <  1:11623099/150‑1 (MQ=255)

             gcTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTaa                                                                                                                                          <  1:8330947/150‑1 (MQ=255)

              cTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTaaa                                                                                                                                         <  1:14034020/150‑1 (MQ=255)

               tGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAg                                                                                                                                        <  2:2695300/150‑1 (MQ=255)

                 ccGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTATTGCGCGGTCGGTTTCGGATGTGTAAATgt                                                                                                                                      >  2:13671267/1‑150 (MQ=255)

                  cGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTAATTCAGGCGCGGTCGGTTGCGGGTGAGTAAAGgtg                                                                                                                                     >  2:3056104/1‑150 (MQ=255)

                      ttGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTAATTCTGGCGCGGTCGGTTGCGGATGGGTTAAGGTGTGGt                                                                                                                                 >  2:4336275/1‑150 (MQ=255)

                        gCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTgg                                                                                                                               <  2:1217263/150‑1 (MQ=255)

                         cATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGc                                                                                                                              <  2:7864107/150‑1 (MQ=255)

                           tttGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTTGTTTTTGGGGGGTGGGTTGGGGTTGTGTTAATGTGTGGTGGGAt                                                                                                                            >  2:648139/1‑150 (MQ=255)

                           tttGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAATTCTGGCGCGGTCGGTTGCGGTTGAGTAAATGTGTCGTGGCAt                                                                                                                            >  2:9768788/1‑150 (MQ=255)

                           tttGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGGGTAAAGGTGTCGTGGCAt                                                                                                                            >  1:12690159/1‑150 (MQ=255)

                                  caccaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAgcg                                                                                                                     <  1:8994074/150‑1 (MQ=255)

                                   accaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAgcgc                                                                                                                    <  2:5278662/150‑1 (MQ=255)

                                    ccaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGcgcg                                                                                                                   <  2:3272146/150‑1 (MQ=255)

                                    ccaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGcgcg                                                                                                                   <  1:3246600/150‑1 (MQ=255)

                                    ccaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACGTATGGCGCGGTCGGGTGCGGAGGAGTAAAGGTGTCGGGGCATAACAgcgcc                                                                                                                   >  2:10986698/1‑149 (MQ=255)

                                        ggCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCaa                                                                                                               <  1:14577901/150‑1 (MQ=255)

                                         gCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAac                                                                                                              <  1:3653718/150‑1 (MQ=255)

                                          ccTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTGATGTGGGGCGGTCGGTTGCGGAGGAGTAAATGTGTGGTGGGATAACAGCGCGGCAaca                                                                                                             >  2:13049054/1‑150 (MQ=255)

                                          ccTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                                                             <  1:15379682/150‑1 (MQ=255)

                                          ccTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTACGTGATGCGCGGTCGGGTGCCGATGACGAAAGTTGTCGTGGACTAAAATCCAGGCAaca                                                                                                             >  1:12401164/1‑150 (MQ=255)

                                            tGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTGGTTTGTAGTGTCGAGTCGGTTTTTGTACGTCTGGCGCGGGCGGGTGCGGCGGAGGAAAGGTGGCGTGGCCGAACAGCGCGGcaccacc                                                                                                           >  2:12729591/1‑149 (MQ=255)

                                              aCGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGTTGAGTAAATGTGGGGTGGCATAACAGCGCGGCAacaccca                                                                                                         >  1:2104219/1‑147 (MQ=255)

                                              aCGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTTATTATGGCGCGGTGGGTTGCGGCTGAGTGAAGGGGGCGTGGCCTAACAGCGCGGCAACACCaa                                                                                                         >  2:7376271/1‑150 (MQ=255)

                                               cGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATGACAGCGCGGCAACACGAAc                                                                                                        <  2:11610865/150‑1 (MQ=255)

                                               cGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAAc                                                                                                        <  2:13240407/150‑1 (MQ=255)

                                                ggCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTCTGGCGCGGTCGGTTGCGGATGTGTAAAGGTGTCGTTGCATAACAGCGCGGCAacaacaaag                                                                                                       >  2:13978784/1‑144 (MQ=255)

                                                ggCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTTCGGTTTAGTAAAGGTTTAGTGGCATAAAAGCGCGGCAACACCAACg                                                                                                       >  2:10815943/1‑150 (MQ=255)

                                                 gCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTATTGCGCGGGGGGTTGCGGAGGGGTAAAAGTGTCGTGGCATAAAAACGCCGCAAAACCAAcct                                                                                                      >  1:13812494/1‑148 (MQ=255)

                                                  cGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTAATTATGGCGCGGGCGGTTTCGGGTGTGTTAAGGTGGCGTGGCCTAACAGCGCGGCAacacccaccga                                                                                                     >  2:4200093/1‑143 (MQ=255)

                                                     cGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTc                                                                                                  <  1:869689/150‑1 (MQ=255)

                                                        tGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGTGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCgat                                                                                               >  2:9105069/1‑150 (MQ=255)

                                                        tGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCgat                                                                                               <  1:2821029/150‑1 (MQ=255)

                                                        tGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTTCTTAGGGGGCGGTGGGGTGCGGATGAGTTAAGGGGTGGGGGCCTAAAAGCCCGGCAACACGAACGTATTAgat                                                                                               >  1:3879815/1‑150 (MQ=255)

                                                         gAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTAGTTTTGGGGCGGTCGGTTGGGGGTGTGTTATGGTGTGGTGGGGTGACAACGCGGCGACACGGACGGTTTCgatg                                                                                              >  2:11917999/1‑150 (MQ=255)

                                                         gAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAATTCTGGCGCGGTCGGGTGCGGGTGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCgatg                                                                                              >  2:4959507/1‑150 (MQ=255)

                                                         gAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTTATTGTGGCGCGGGCGGGTGGGGGTGAGTAAAGTTGTGGTGGGATAAAAGCGCGGCAACACCAACGTAGTAgatg                                                                                              >  1:7273542/1‑150 (MQ=255)

                                                          aaaCTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTGTTTTTTATTCTGGCGCGGGGGGGTGCGGAGGGTTAAATGTGTGGGGGCATAACAGCGCGGCAAAACCAACGTATTAgatgt                                                                                             >  1:7984198/1‑149 (MQ=255)

                                                                ttGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCtt                                                                                       <  1:5628411/150‑1 (MQ=255)

                                                                 tGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTc                                                                                      <  1:4874163/150‑1 (MQ=255)

                                                                   ccGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGGGCGGTCGGGTGCGGGGGGGTGAAGGTGTGGTGGCCTAACAGCGCGGCAACACGCACGGATTCGGTGATCTCTTCaa                                                                                    >  2:1679952/1‑150 (MQ=255)

                                                                   ccGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCaa                                                                                    <  1:13249683/150‑1 (MQ=255)

                                                                   ccGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACATATTGCGCGGTCGGGTGCGGCTGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAAACGATTCGAGTATCTCTTaaa                                                                                    >  2:9530163/1‑150 (MQ=255)

                                                                    cGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTTCTTCTGGCGCGGTCGGGTGCGGGTGAGTAAAGGTGTGGTGGGATAAAAGCGCGGCCACACCAACGTATTCGCGGATATCTTaaaa                                                                                   >  1:1739020/1‑149 (MQ=255)

                                                                       gATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGa                                                                                <  2:6750613/150‑1 (MQ=255)

                                                                             tcactcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACGTCAGCCCCCGTCGGTTGCCGCTGAGGAAAGGTGTCCTGGCCTAACACCGCGGCAAAACCAACGGATTCGAAGATCGCTACAACACACATTTc                                                                          >  2:8283717/1‑150 (MQ=255)

                                                                               actcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCaa                                                                        <  2:5875676/150‑1 (MQ=255)

                                                                               actcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCaa                                                                        <  1:10398190/150‑1 (MQ=255)

                                                                               actcaTAACACTCTCCTGACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGTGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCaa                                                                        <  2:10618553/150‑1 (MQ=255)

                                                                                      acacTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTc                                                                 <  1:6319966/150‑1 (MQ=255)

                                                                                           ctcCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAt                                                            <  1:522904/150‑1 (MQ=255)

                                                                                              cTTCCGTTTTTTGTTTTTTGTGTAGAGTCGGTGTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGACCGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtctt                                                         <  2:3946334/150‑1 (MQ=255)

                                                                                               ttACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttc                                                        <  2:14834198/150‑1 (MQ=255)

                                                                                                 aCGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgt                                                      <  2:6249661/150‑1 (MQ=255)

                                                                                                    ttttttGTTTTTAGTGTAGAGTCGGTTTTTTTACTTCTGGCGCGGTCGGTTTCGGATTAGTAAATGTTTAGTGGCATAACAGCGCGGCAAAACCAACGGAATAGATGATCTCATAAATCGACAATTCAAGCTCTTACAGAtattagtctt                                                   >  2:15397402/1‑150 (MQ=255)

                                                                                                    ttttttGTTTTTAGTGTAGAGTCGGTTTTTTTACTTATGGCGCGGTCGGTTTCGGCGGAGTAAATGTGTCGTGGCCAAAAAGCGCGGCAACACAAAAGTAATAGATGATATATTAAAGCGACAAATAAAACTCTTCCAGAtattagtcgt                                                   >  2:5482506/1‑148 (MQ=255)

                                                                                                     tttttGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtcttc                                                  <  1:10343310/150‑1 (MQ=255)

                                                                                                     tttttGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtcttc                                                  <  2:5662461/150‑1 (MQ=255)

                                                                                                         tGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTtcgtcg                                              <  2:3652846/150‑1 (MQ=255)

                                                                                                           tttttAGTGTAGAGTCGGTTTTTTTACGTATGGCGCGGTCGGTTGCGGGTGAGGAAATGTGTGGTGGCAAAACAGCGCGGCAACACCAACGTATTCGATTAAGACTTAAAACGACATTTCAAGCTCATACTGTTGTTCGTCTtcgtcgga                                            >  2:7549454/1‑148 (MQ=255)

                                                                                                            ttttAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTaa                                           <  1:4336275/150‑1 (MQ=255)

                                                                                                            ttttAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTaa                                           <  1:851601/150‑1 (MQ=255)

                                                                                                            ttttAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTaa                                           <  1:15019476/150‑1 (MQ=255)

                                                                                                             tttAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAATGTGTCGTGGCATAACAGCGCGGCAACACCAACGTATTAGATGATATCTTCAATCGACAATTAAAGCTCATCCAGATCTTAGTCTTCGTCGTAAc                                          >  2:5173586/1‑150 (MQ=255)

                                                                                                             tttAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAAc                                          <  1:670269/150‑1 (MQ=255)

                                                                                                              ttAGTGTAGAGTCGGTTTTTTTACATATGGCGCGGTCGGTTTCGGATGAGTAAAGGTGTCGTGGCATAAAAGCGCGGCAACACGAAACTATTAGATGATCTCTTAAAGCGACAATTAAAGCTCTTACAGATAATAGTCTTAGTCGTaaac                                         >  2:208225/1‑148 (MQ=255)

                                                                                                                aGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCa                                       <  1:10720950/150‑1 (MQ=255)

                                                                                                                   gtAGAGTCGGTTTTTTTAAATCTTGCGCGGTCGGTTGCGGATGAGTAAAGGTTTCGTGGCAAAAAAGCGCGGCAACACCAAACTAATAGAAGATAACTTCAAGCGACAATTCAACCACTTACAGATCTTCGTCGTCGTCGTAACCCAGtt                                    >  2:1015963/1‑150 (MQ=255)

                                                                                                                   gtAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGtt                                    <  1:7522349/150‑1 (MQ=255)

                                                                                                                   gtAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGtt                                    <  1:11018429/150‑1 (MQ=255)

                                                                                                                       agTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAATGGTGTCGTGGCATAACAGCGCGGCAACACGTACGTATTCGATGATCTCTTCAAGCGACATTTCACGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTgcgc                                <  1:10141893/150‑1 (MQ=255)

                                                                                                                         tCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTACAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCaa                              >  2:9282531/1‑150 (MQ=255)

                                                                                                                         tCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCaa                              >  2:8701526/1‑150 (MQ=255)

                                                                                                                         tCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCaa                              >  2:14184292/1‑150 (MQ=255)

                                                                                                                         tCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCaa                              >  2:1132343/1‑150 (MQ=255)

                                                                                                                          cGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAAt                             <  1:9287230/150‑1 (MQ=255)

                                                                                                                            gTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGt                           >  1:10015861/1‑150 (MQ=255)

                                                                                                                            gTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGt                           <  1:608278/150‑1 (MQ=255)

                                                                                                                            gTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGt                           >  1:15266100/1‑150 (MQ=255)

                                                                                                                              ttttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTa                         <  2:6152651/150‑1 (MQ=255)

                                                                                                                              ttttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTa                         <  2:14363375/150‑1 (MQ=255)

                                                                                                                               tttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTAc                        >  1:4683302/1‑150 (MQ=255)

                                                                                                                                ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACg                       <  1:12804123/150‑1 (MQ=255)

                                                                                                                                      ttCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGAtc                 >  1:2381345/1‑150 (MQ=255)

                                                                                                                                        cTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGAtcgt               <  1:12236964/150‑1 (MQ=255)

                                                                                                                                        cTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGAtcgt               <  1:14122133/150‑1 (MQ=255)

                                                                                                                                         tGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGAtcgtc              <  2:7180585/150‑1 (MQ=255)

                                                                                                                                         tGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGAtcgtc              <  1:13101634/150‑1 (MQ=255)

                                                                                                                                         tGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGAtcgtc              >  2:8495214/1‑150 (MQ=255)

                                                                                                                                          ggCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGAtcgtcg             <  1:7870783/150‑1 (MQ=255)

                                                                                                                                                  cggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTc     >  2:1521335/1‑150 (MQ=255)

AAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACC  >  NC_000913/3054756‑3055054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`