Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,095,145 A→C 9.3% S16S (TCA→TCC yggS → UPF0001 family protein, PLP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,095,1450AC 9.3% 2555.2 / 70.8 869S16S (TCA→TCCyggSUPF0001 family protein, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base A (401/366);  new base C (2/93);  total (409/460)
Fisher's exact test for biased strand distribution p-value = 3.60e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 884 total aligned reads displayed.

GCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTAGATAAAA  >  NC_000913/3094996‑3095293
                                                                                                                                                     |                                                                                                                                                    
gcCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCa                                                                                                                                                      <  2:11670501/150‑1 (MQ=255)
  cATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCCGc                                                                                                                                                    <  1:7616965/150‑1 (MQ=255)
     cGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGc                                                                                                                                                 <  1:7984886/150‑1 (MQ=255)
         cGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACACTTTCTTCCATATTCGGCCTAAGGTCCTCGGACAATGAACGAGATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCGGCGGCTGCa                                                                                                                                             >  2:3231970/1‑150 (MQ=255)
          gggCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCaa                                                                                                                                            <  2:10611124/150‑1 (MQ=255)
          gggCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCaa                                                                                                                                            >  2:12723218/1‑150 (MQ=255)
             ccAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCGTTACACTAAGGGCCACAATTTCTTCCCTATTCCTACGAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCCCACGTCCGGGACAAAATCTCAGCGGCTGCAAcgc                                                                                                                                         >  1:11832189/1‑150 (MQ=255)
              cAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCgg                                                                                                                                                  <  2:5320074/140‑1 (MQ=255)
              cAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCACGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCgg                                                                                                                                                  >  1:5320074/1‑140 (MQ=255)
               aGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGt                                                                                                                                       <  2:2287916/150‑1 (MQ=255)
                 gcgcTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTg                                                                                                                                     <  2:3461820/150‑1 (MQ=255)
                 gcgcTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTg                                                                                                                                     <  2:11330619/150‑1 (MQ=255)
                 gcgcTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTg                                                                                                                                     >  1:13112069/1‑150 (MQ=255)
                  cgcTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCCGCGGCTGCAACGCGTTGc                                                                                                                                    <  1:8936254/150‑1 (MQ=255)
                      gCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGcc                                                                                                                                >  2:4691698/1‑150 (MQ=255)
                          aGGTGTAGATCCTAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGTAAAATTAACTATTTTGCGCATAACCTGGCACTGGTCCGGGACAAAATCTCCGCGGCTGCAACGCGTTGCGGCCGTtc                                                                                                                            <  1:7787602/150‑1 (MQ=255)
                            gtgtAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTtctc                                                                                                                          >  2:12123641/1‑150 (MQ=255)
                            gtgtAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTtctc                                                                                                                          <  2:1760290/150‑1 (MQ=255)
                             tgtAGATCCGAGACGTTTTGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTACGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACTGGTCCGGGACAAAATCTCCGCGGCTGCAACGCGTTGCGGCCGTTCTcc                                                                                                                         <  1:12585918/150‑1 (MQ=255)
                              gtAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCCTATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCa                                                                                                                        >  1:8698194/1‑150 (MQ=255)
                               tAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCag                                                                                                                       <  2:12464038/150‑1 (MQ=255)
                               tAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCag                                                                                                                       >  2:9791888/1‑150 (MQ=255)
                                  aTCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTTCGCATAACCAGGCACAGGTCCGGGACAAAATATCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCagaag                                                                                                                    >  1:4464604/1‑150 (MQ=255)
                                      gagaCGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAAt                                                                                                                >  1:9833982/1‑150 (MQ=255)
                                       agaCGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAAtt                                                                                                               >  2:14756272/1‑150 (MQ=255)
                                        gaCTTTATGCTTTACACTACGGGCCACAATTTCTTCAATATTCATACTAAGATCCTCGGAAAATTAACGATATTGCGCATAACCTGGCTCAGGTCCGGGACAAAATCTCCGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTa                                                                                                              <  1:2478626/150‑1 (MQ=255)
                                           gTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACgc                                                                                                           >  1:3364886/1‑150 (MQ=255)
                                             tATGCTTTACACTAAGGGCCACAATTTCTGCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACgctg                                                                                                         >  1:11004795/1‑150 (MQ=255)
                                              aTGCTTTACACTAAGGGCCACATTTTCTTCCTTATTCATAATAAGATCCTCGTTAAATTAACGTTTTTGCGATTAACCTGTAACTGGTCGGGTAAAAATTCTCCGCGGCTGCAACGCGTTGCGGCCTTTCTCCAGAAGAAATTACgctgc                                                                                                        <  1:841044/150‑1 (MQ=255)
                                               tGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACgctgct                                                                                                       >  2:10371106/1‑150 (MQ=255)
                                                gCTTTACACTAAGGTCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAAGTATTTTGCGCATAACATGGAACAGGTCCTGGACAAATTCTCAGCGGCTGCAAAGCGTTGCTGCCGTTCTCCAGAAGAAATTACGCTGCtt                                                                                                      <  1:10021729/150‑1 (MQ=255)
                                                 cTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTg                                                                                                     <  2:8064837/150‑1 (MQ=255)
                                                 cTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGAAAAAATCTCCGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTg                                                                                                     <  1:13307688/150‑1 (MQ=255)
                                                  tttACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGc                                                                                                    >  1:12475743/1‑150 (MQ=255)
                                                   ttACACTACGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGca                                                                                                   <  1:14099753/150‑1 (MQ=255)
                                                   ttACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGca                                                                                                   <  2:8524566/150‑1 (MQ=255)
                                                     acacTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGcagt                                                                                                 <  2:5884390/150‑1 (MQ=255)
                                                       acTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGcagtca                                                                                               <  1:719947/150‑1 (MQ=255)
                                                       acTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGcagtca                                                                                               <  2:14024047/150‑1 (MQ=255)
                                                         tAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGcagtcagt                                                                                             <  2:2548031/150‑1 (MQ=255)
                                                         tAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGcagtcagt                                                                                             >  1:5940449/1‑150 (MQ=255)
                                                         tAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGcagtcagt                                                                                             >  2:15428731/1‑150 (MQ=255)
                                                           aGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTaa                                                                                           >  2:308444/1‑150 (MQ=255)
                                                            gggCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTaaa                                                                                          >  2:840501/1‑150 (MQ=255)
                                                            gggCCACAATTTCGTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTaaa                                                                                          <  1:15321986/150‑1 (MQ=255)
                                                                 acaATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGca                                                                                                   <  1:284163/136‑1 (MQ=255)
                                                                  caATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGTAGTCAGTaaaacaaaa                                                                                    <  2:11949323/150‑1 (MQ=255)
                                                                       tcttcCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGc                                                                               >  1:930192/1‑150 (MQ=255)
                                                                         ttcCATATACATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGa                                                                             <  1:14329242/150‑1 (MQ=255)
                                                                          tcCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAg                                                                            >  1:12794581/1‑150 (MQ=255)
                                                                             atatTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAgcgc                                                                         >  1:12216516/1‑150 (MQ=255)
                                                                             atatTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAgcgc                                                                         >  1:4897511/1‑150 (MQ=255)
                                                                                ttCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCAt                                                                      >  1:13884843/1‑150 (MQ=255)
                                                                                ttCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCAt                                                                      >  1:3731720/1‑150 (MQ=255)
                                                                                  cATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCg                                                                    <  1:4610413/150‑1 (MQ=255)
                                                                                    tACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCa                                                                  >  2:14372951/1‑150 (MQ=255)
                                                                                    tACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCa                                                                  >  2:555598/1‑150 (MQ=255)
                                                                                     aCTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAg                                                                 >  1:2239108/1‑150 (MQ=255)
                                                                                     aCTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAg                                                                 >  2:8966056/1‑150 (MQ=255)
                                                                                      cTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGa                                                                <  2:4272080/150‑1 (MQ=255)
                                                                                       tAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGaa                                                               >  1:3962952/1‑150 (MQ=255)
                                                                                       tAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTGGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGaa                                                               >  2:13133025/1‑150 (MQ=255)
                                                                                       tAAGATCCTACTAAAATGAACTAAATTTAGAATAACCTGTCACAGGACCGGTAAAAAATCTAAGCTGCTGCAACGAGATCCGGCCGTTCTCCAGAAAAAATTAAGATCCTTGCAGTCAGTAAAACAAAAACAGCGAGCGCCATCGCACaa                                                               >  1:13616605/1‑150 (MQ=255)
                                                                                            tCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCAt                                                          <  1:15498980/150‑1 (MQ=255)
                                                                                            tCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCAt                                                          <  1:6372034/150‑1 (MQ=255)
                                                                                             ccTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCAtt                                                         >  2:1980453/1‑150 (MQ=255)
                                                                                              cTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTg                                                        >  2:639793/1‑150 (MQ=255)
                                                                                              cTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTg                                                        >  1:1079806/1‑150 (MQ=255)
                                                                                               tCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGGAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGa                                                       >  1:6063584/1‑150 (MQ=255)
                                                                                               tCGGAAAATGAACGATAATGCGCATAACCAGGAACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAATAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGa                                                       >  2:4461695/1‑150 (MQ=255)
                                                                                                 ggAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATg                                                     >  2:7735874/1‑150 (MQ=255)
                                                                                                  gAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGc                                                    >  2:614336/1‑150 (MQ=255)
                                                                                                     aaTGAACGATATTGCGCATAACCTCGCACACGTCCGGGACAAAATCTCACCGCCAGCAACCCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCgg                                                 <  2:7454950/150‑1 (MQ=255)
                                                                                                          aCGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGc                                            <  1:2077710/150‑1 (MQ=255)
                                                                                                           cGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCcgcg                                           >  2:14121797/1‑150 (MQ=255)
                                                                                                                ttGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGCAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCg                                                                    >  1:14941536/1‑120 (MQ=255)
                                                                                                                ttGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCg                                                                    <  2:14941536/120‑1 (MQ=255)
                                                                                                                   cgcATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTg                                   >  1:3089271/1‑150 (MQ=255)
                                                                                                                    gcATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTgg                                  <  1:10665840/150‑1 (MQ=255)
                                                                                                                          ccTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGaaaa                            <  1:1019376/150‑1 (MQ=255)
                                                                                                                          ccTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGaaaa                            <  2:2834271/150‑1 (MQ=255)
                                                                                                                           cTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAAc                           <  2:3811621/150‑1 (MQ=255)
                                                                                                                            tGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTg                                                                                >  2:15258519/1‑96 (MQ=255)
                                                                                                                              gCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTAc                        <  2:10853717/150‑1 (MQ=255)
                                                                                                                                    gTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCAt                                                          <  2:9493374/110‑1 (MQ=255)
                                                                                                                                    gTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAg                  >  2:6985774/1‑150 (MQ=255)
                                                                                                                                    gTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAg                  <  2:13520949/150‑1 (MQ=255)
                                                                                                                                     tCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAgg                 <  1:6552779/150‑1 (MQ=255)
                                                                                                                                         ggACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAgg             >  1:9636948/1‑150 (MQ=255)
                                                                                                                                          gACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAggg            <  1:11990595/150‑1 (MQ=255)
                                                                                                                                           aCAAAATCTCCGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAgggg           <  1:14770152/150‑1 (MQ=255)
                                                                                                                                           aCAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAgggg           <  1:4236787/150‑1 (MQ=255)
                                                                                                                                            cAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGt          >  1:8295840/1‑150 (MQ=255)
                                                                                                                                            cAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGt          >  1:11420445/1‑150 (MQ=255)
                                                                                                                                            cAAAATCTCAGCCGCAGCAACGCGTAGCGGCCGATCTCCAGAAGAAATTACGCTGCTTGCAGACAGTAAAACAAAACCTGCGAGCGCCAACGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGt          <  2:11093860/150‑1 (MQ=255)
                                                                                                                                             aaaaTCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTa         >  2:7256513/1‑150 (MQ=255)
                                                                                                                                             aaaaTCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATGTGGTGAAAACTACGTTCAGGAAGGGGTa         >  2:10079852/1‑150 (MQ=255)
                                                                                                                                               aaTCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTAGa       <  1:5807326/150‑1 (MQ=255)
                                                                                                                                               aaTCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTAGa       >  1:5525349/1‑150 (MQ=255)
                                                                                                                                                   tcAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTAGATaaa   >  2:4968124/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCCATCGTGCGGGCCAGGCGCTGCACAGGTGTAGATCCGAGACGTTATGCTTTACACTAAGGGCCACAATTTCTTCCATATTCATACTAAGATCCTCGGAAAATGAACGATATTGCGCATAACCTGGCACAGGTCCGGGACAAAATCTCAGCGGCTGCAACGCGTTGCGGCCGTTCTCCAGAAGAAATTACGCTGCTTGCAGTCAGTAAAACAAAACCTGCGAGCGCCATCGCAGAAGCCATTGATGCCGGGCAGCGTCAATTTGGTGAAAACTACGTTCAGGAAGGGGTAGATAAAA  >  NC_000913/3094996‑3095293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: