BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,180,505	G→T	5.0%	E29* (GAG→TAG) ‡	ygiC →	ATP‑Grasp family ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,180,505	0	G	T	5.0%	3555.9 / 108.8	988	E29* (GAG→TAG) ‡	ygiC	ATP‑Grasp family ATPase
Reads supporting (aligned to +/- strand): ref base G (469/467); new base T (32/20); total (501/487)
Fisher's exact test for biased strand distribution p-value = 1.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 995 total aligned reads displayed.

AAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATT  >  NC_000913/3180356‑3180654

aaacaaaGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCg                                                                                                                                                        >  1:13462213/1‑150 (MQ=255)

aaacaaaGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCg                                                                                                                                                        >  2:8546404/1‑150 (MQ=255)

  acaaaGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAg                                                                                                                                                      <  1:10091777/150‑1 (MQ=255)

   caaaGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCTTGTACGGCGAGc                                                                                                                                                     <  2:10676525/150‑1 (MQ=255)

       gCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTa                                                                                                                                                 <  1:12962013/150‑1 (MQ=255)

       gCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTa                                                                                                                                                 <  1:10570561/150‑1 (MQ=255)

         aCTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGTCTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTATCACACCATGTACGGAGAGCCGTAC‑t                                                                                                                                              >  2:7186943/1‑150 (MQ=255)

          cTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGATAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑Tg                                                                                                                                             <  1:9628299/150‑1 (MQ=255)

           tATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑Tgg                                                                                                                                            >  1:12534798/1‑150 (MQ=255)

           tATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑Tgg                                                                                                                                            <  1:14911225/150‑1 (MQ=255)

            aTGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGgt                                                                                                                                           <  2:3775894/150‑1 (MQ=255)

            aTGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGgt                                                                                                                                           <  2:1093772/150‑1 (MQ=255)

             tGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGgtg                                                                                                                                          >  2:3530597/1‑150 (MQ=255)

              gccgCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGgtgt                                                                                                                                         >  1:3852193/4‑150 (MQ=255)

                 gCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTTATACCGATGGAAAGTGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGaa                                                                                                                                      <  1:348181/150‑1 (MQ=255)

                      gTGCAACCGGTACCTCTTCTCGTTCCATGGGTGGCTGATACCTATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGATAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGc                                                                                                                                 <  1:9159080/150‑1 (MQ=255)

                       tGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCt                                                                                                                                >  1:1684090/1‑150 (MQ=255)

                          aaCCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTtac                                                                                                                             <  1:12721114/150‑1 (MQ=255)

                           aCCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTtact                                                                                                                            >  1:696390/1‑150 (MQ=255)

                           aCCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTtact                                                                                                                            >  2:7727660/1‑150 (MQ=255)

                            ccGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTtacta                                                                                                                           <  1:5501242/150‑1 (MQ=255)

                               gTACCTCTTCTCGTTCTATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACaa                                                                                                                        <  1:9852943/150‑1 (MQ=255)

                               gTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGATGATGCTTACTACaa                                                                                                                        <  2:9721931/150‑1 (MQ=255)

                               gTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACaa                                                                                                                        <  2:2073742/150‑1 (MQ=255)

                                tACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAg                                                                                                                       <  2:6728876/150‑1 (MQ=255)

                                 aCCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGt                                                                                                                      <  1:8708258/150‑1 (MQ=255)

                                   ctctTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTg                                                                                                                    >  1:14223136/1‑150 (MQ=255)

                                    tcttctCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGa                                                                                                                   >  1:15411135/1‑150 (MQ=255)

                                    tcttctCGTTCAAGGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGa                                                                                                                   <  2:15390812/150‑1 (MQ=255)

                                    tcttctCGGTCGATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTAGCGAGCGCCCGGACGGGCGGGAGAAAGCCCACGAATACGGTTTCAATTGTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGa                                                                                                                   >  2:14502244/1‑150 (MQ=255)

                                        ctcGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCt                                                                                                               >  1:4714012/1‑150 (MQ=255)

                                         tcGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTc                                                                                                              >  1:8406785/1‑150 (MQ=255)

                                           gTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGc                                                                                                            >  2:9054269/1‑150 (MQ=255)

                                                aTGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAgg                                                                                                       <  2:3191238/150‑1 (MQ=255)

                                                    gTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGa                                                                                                   >  2:14825987/1‑150 (MQ=255)

                                                       gCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGaaaa                                                                                                <  1:11947090/150‑1 (MQ=255)

                                                         tGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGc                                                                                              >  2:7377597/1‑150 (MQ=255)

                                                         tGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGc                                                                                              <  2:2403196/150‑1 (MQ=255)

                                                          gATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCt                                                                                             <  1:5171473/150‑1 (MQ=255)

                                                           aTACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTg                                                                                            <  2:14166291/150‑1 (MQ=255)

                                                              ccGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaa                                                                                         <  2:5074822/150‑1 (MQ=255)

                                                               cGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaag                                                                                        >  1:1909902/1‑150 (MQ=255)

                                                                 attgAAAGAGTCAGTTTTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaagaa                                                                                      <  1:2656672/147‑1 (MQ=255)

                                                                 aTGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaagaa                                                                                      <  1:4919145/150‑1 (MQ=255)

                                                                  tGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGaagaag                                                                                     <  2:1235940/150‑1 (MQ=255)

                                                                     aaaGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGcc                                                                                                           <  1:3203952/125‑1 (MQ=255)

                                                                     aaaGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCa                                                                                  <  2:13118950/150‑1 (MQ=255)

                                                                         agTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCAccgc                                                                              <  2:2987774/150‑1 (MQ=255)

                                                                          gTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCAccgcc                                                                             <  2:8747622/150‑1 (MQ=255)

                                                                               tATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACt                                                                        >  2:11250541/1‑150 (MQ=255)

                                                                                 ttACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGc                                                                      <  2:10393727/150‑1 (MQ=255)

                                                                                 ttACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGc                                                                      >  1:13932793/1‑150 (MQ=255)

                                                                                  tACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGTGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCa                                                                     <  2:11664401/150‑1 (MQ=255)

                                                                                  tACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCa                                                                     >  1:10809949/1‑150 (MQ=255)

                                                                                   aCCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCAc                                                                    <  1:6252783/150‑1 (MQ=255)

                                                                                    ccGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaa                                                                                         >  2:4517952/1‑128 (MQ=255)

                                                                                     cGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGTGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCAccc                                                                  >  1:11657016/1‑149 (MQ=255)

                                                                                     cGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGgt                                                                                                                                           >  2:2643551/1‑77 (MQ=255)

                                                                                      gAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATACTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAg                                                                 <  2:13284149/150‑1 (MQ=255)

                                                                                       aGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGa                                                                >  2:5563521/1‑150 (MQ=255)

                                                                                       aGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGa                                                                <  2:12893527/150‑1 (MQ=255)

                                                                                       aGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGa                                                                <  2:1567928/150‑1 (MQ=255)

                                                                                          gcCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGAtgt                                                             <  1:2999523/150‑1 (MQ=255)

                                                                                           cccGGACTGGCGTGAGAAAGCCCACGAATACTGTTTCAATTTTCACACCATGTACGGCGATCCGTAC‑TGGTGTGAAGATTCTTACTACAAGTTGACGCTCGCCCAGGTTGAAAAGCTGGAAGAAGTAACCGCAGAACTGCACCAGAtgtg                                                            >  2:12550946/1‑150 (MQ=255)

                                                                                            ccGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGc                                                           <  2:13880854/150‑1 (MQ=255)

                                                                                            ccGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGc                                                           >  2:1896195/1‑150 (MQ=255)

                                                                                            ccGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGc                                                           <  2:15088390/150‑1 (MQ=255)

                                                                                             cGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAg                                                                                                                       >  1:4499167/1‑89 (MQ=255)

                                                                                              ggACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCt                                                         <  2:1204287/150‑1 (MQ=255)

                                                                                                    gCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgt                                                   >  2:13567806/1‑150 (MQ=255)

                                                                                                       tGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtggt                                                <  2:7955615/150‑1 (MQ=255)

                                                                                                       tGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtggt                                                <  1:9383711/150‑1 (MQ=255)

                                                                                                        gagaAAGCCCACGAATTCGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtggtg                                               <  1:7186943/150‑1 (MQ=255)

                                                                                                        gagaAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaagaa                                                                                      <  1:11445820/111‑1 (MQ=255)

                                                                                                        gagaAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaagaa                                                                                      >  2:11445820/1‑111 (MQ=255)

                                                                                                          gaAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCt                                                                                             <  1:12445307/102‑1 (MQ=255)

                                                                                                              gCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGaaaaa                                         <  2:8133137/150‑1 (MQ=255)

                                                                                                                ccACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGt                                       >  1:6523347/1‑150 (MQ=255)

                                                                                                                 cACGAATACGGTTTCAATTTTCACACCAGGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTg                                      <  1:7547210/150‑1 (MQ=255)

                                                                                                                  aCGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGa                                     >  2:10264292/1‑150 (MQ=255)

                                                                                                                              tCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATg                         >  1:2379920/1‑150 (MQ=255)

                                                                                                                               gcatcttCACACCA‑GTACGGCTCGCCGTACATGGTGTGAAAATGCTTACTACAAGTTGACCCACGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGa                        >  1:15144898/6‑150 (MQ=37)

                                                                                                                               cAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGa                        <  1:13692283/150‑1 (MQ=255)

                                                                                                                                aaTTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAg                       >  2:4095274/1‑150 (MQ=255)

                                                                                                                                   tttCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCtg                    <  2:11804509/150‑1 (MQ=255)

                                                                                                                                    ttCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCtga                   >  1:13540999/1‑150 (MQ=255)

                                                                                                                                     tCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCtgat                  <  2:696390/150‑1 (MQ=255)

                                                                                                                                     tCACACCA‑GTACGGCTCGCCGTACATGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCtgat                  >  2:5356369/1‑150 (MQ=255)

                                                                                                                                       acacCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCtgatga                >  1:11045082/1‑150 (MQ=255)

                                                                                                                                         caccagTACGGCTCGCCGTACATGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAc               >  2:6153725/6‑150 (MQ=255)

                                                                                                                                         acCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAcc              <  2:9406482/150‑1 (MQ=255)

                                                                                                                                         acCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAcc              >  1:8075184/1‑150 (MQ=255)

                                                                                                                                          ccATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCa             >  1:7835919/1‑150 (MQ=255)

                                                                                                                                          ccATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCa             >  1:5826970/1‑150 (MQ=255)

                                                                                                                                          accagTACGGCTCGCCGTACATGGTGTGAAAATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAcc              >  1:13248615/5‑150 (MQ=255)

                                                                                                                                           cATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCaa            >  1:4247756/1‑150 (MQ=255)

                                                                                                                                            aTGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGAt                          <  1:10356000/135‑1 (MQ=255)

                                                                                                                                              gTACGGCTCGCCGTACATGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAAt          >  1:2941965/1‑150 (MQ=255)

                                                                                                                                                 cGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCg      <  1:14499326/150‑1 (MQ=255)

                                                                                                                                                  ggCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGc     >  2:836709/1‑150 (MQ=255)

AAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATT  >  NC_000913/3180356‑3180654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`