Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,532 C→A 7.2% V69L (GTA→TTA)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,5320CA 7.2% 1767.6 / 16.9 592V69L (GTA→TTA) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (280/245);  new base A (37/30);  total (317/275)
Fisher's exact test for biased strand distribution p-value = 7.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 602 total aligned reads displayed.

ATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATA  >  NC_000913/238383‑238681
                                                                                                                                                     |                                                                                                                                                     
 tatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACa                                                                                                                                                      <  1:4632735/150‑1 (MQ=255)
    ccTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGa                                                                                                                                                   <  2:9117248/150‑1 (MQ=255)
     cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGaa                                                                                                                                                  >  2:4450094/1‑150 (MQ=255)
     cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGaa                                                                                                                                                  >  2:11724955/1‑150 (MQ=255)
     cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGaa                                                                                                                                                  >  1:5462998/1‑150 (MQ=255)
      tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                                                 >  2:8810940/1‑150 (MQ=255)
        tgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAatat                                                                                                                                               >  2:6216748/1‑150 (MQ=255)
          aCAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATAttt                                                                                                                                             >  1:4509102/1‑150 (MQ=255)
             aaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaa                                                                                                                                          >  2:10900497/1‑150 (MQ=255)
              aaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaa                                                                                                                                         <  1:10173274/150‑1 (MQ=255)
               aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                                        <  1:13721002/150‑1 (MQ=255)
                ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAg                                                                                                                                       <  1:14080925/150‑1 (MQ=255)
                ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAg                                                                                                                                       <  1:1535268/150‑1 (MQ=255)
                 gAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAgg                                                                                                                                      >  1:14335666/1‑150 (MQ=255)
                    cTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaa                                                                                                                                   >  1:8278318/1‑150 (MQ=255)
                     tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaaa                                                                                                                                  <  1:11603033/150‑1 (MQ=255)
                      aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAc                                                                                                                                 >  2:7068969/1‑150 (MQ=255)
                        tAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGa                                                                                                                               <  2:3888293/150‑1 (MQ=255)
                            ggTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATg                                                                                                                           >  1:1165199/1‑150 (MQ=255)
                              tGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGaa                                                                                                                         >  1:11871607/1‑150 (MQ=255)
                               gCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGaaa                                                                                                                        >  2:3350153/1‑150 (MQ=255)
                                atATTACTATAGATATTATTAAAAATATAAGTGTTAGCTGTATTTTTATGTCTGTGGCTAAATTGAATACATTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAc                                                                                                                       <  2:11308414/149‑1 (MQ=255)
                                 tATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAcc                                                                                                                      >  2:11622724/1‑150 (MQ=255)
                                 tATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAcc                                                                                                                      >  2:12292235/1‑150 (MQ=255)
                                 tATTACGATAGCTATTAGTAAAAATATAAGAGGTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGGATGATTTTATTTTAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACCTGAAAcc                                                                                                                      >  1:10641247/1‑150 (MQ=255)
                                  aTTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAATGAAAAAGACATGAAACCg                                                                                                                     >  1:12104322/1‑150 (MQ=255)
                                   ttACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGa                                                                                                                    >  1:5970687/1‑150 (MQ=255)
                                     aCGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAATGAAAACGACAAGAAAACGAAg                                                                                                                  >  1:7269187/1‑150 (MQ=255)
                                       gATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGca                                                                                                                >  2:3003889/1‑150 (MQ=255)
                                         tAGCTATTTGTAAAATTATATGAGTTAGCTTTATTGTTTTGTCTGTGGCGAAATTTACTACCTTCGTTTTTTTGATTATGATTGTTTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGcaca                                                                                                              <  2:1968768/150‑1 (MQ=255)
                                           gCTATTAGTAAAAATATACGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGa                                                                                                            <  2:12585727/150‑1 (MQ=255)
                                               ttattaAAAATATTTGAGTTCGTTTTTTTTTTTTGTGTGTGGCTAATTTGACTAATTTCTTTTTTTTTTTTTATATTTATTTTATTATTTTAATAAATATTAATTTAATTTTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCa                                                                                                        <  1:7853561/150‑1 (MQ=255)
                                                 aGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAAc                                                                                                      <  2:3569434/150‑1 (MQ=255)
                                                  gTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTTAGATTGTTTTTATTATCGTTAGTAAAATTTCTTGTATTTTTTTATAGGTAATGGTCATGTATGCGAAGCTCAGAAtgatct                                                                                                     >  2:14027797/1‑145 (MQ=255)
                                                   tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAt                                                                                                    >  2:242027/1‑150 (MQ=255)
                                                    aaaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAtt                                                                                                   >  2:12933117/1‑150 (MQ=255)
                                                     aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtc                                                                                                  >  1:6512559/1‑150 (MQ=255)
                                                      aaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAATCACAGAATCAACATtct                                                                                                 >  2:11898505/1‑150 (MQ=255)
                                                       aaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAATGAAAAAGACATGAAACAGAAGCACAGAATCAACATtatc                                                                                                >  2:2710487/1‑150 (MQ=255)
                                                       aaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                                                <  2:13673176/150‑1 (MQ=255)
                                                         tataATTGTTAGCTGTAGTGTTAGGTCTGTGGCGAATTTGACTACCTTCTTTTTTTTGATTAACAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCa                                                                                              <  2:13052817/150‑1 (MQ=255)
                                                               agTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATa                                                                                        >  2:5375323/1‑150 (MQ=255)
                                                                 ttcgCTGTATTGTTTTGTCTGTGGCTAAATTGACTACCTTCGTTTTTTTTATTAAGAATTATTTTATTATCTTAAGTAAAATTACATTAATTTTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATaaa                                                                                      <  1:3423401/147‑1 (MQ=255)
                                                                    gCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATTAAAATTAAATGAATATTTAAAAATGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAata                                                                                   >  2:14343081/1‑150 (MQ=255)
                                                                    gCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAata                                                                                   >  2:8118386/1‑150 (MQ=255)
                                                                      tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTAAGAATGATTTTATTATAGTAAGTAAAATTAAAATAATATTTAAAAATGAAAAACAAAAGAAAACGAAGCACAGAAAAAAAAATATCCAATAAAAAAATAtt                                                                                 >  1:13275221/1‑150 (MQ=255)
                                                                       ttATTGTTATGTCTCTGGCTAAATTTACTACCTTCGTTTTTTTTATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAttt                                                                                <  2:10763427/149‑1 (MQ=255)
                                                                       gTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAttt                                                                                <  2:7988170/150‑1 (MQ=255)
                                                                        tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTATAGTAATTAAAAATAAATTAATATTTAAAAATGAAAAAGAAATGAAAACGAAGCACAGAATAAAAAAAATACAAACAAAAAATAttta                                                                               >  1:8007154/1‑149 (MQ=255)
                                                                            gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATAGTAAGTAAAAATAAATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAAACAAAAATCTCCAATAAAAAAATATTTACGTg                                                                           >  1:2256869/1‑150 (MQ=255)
                                                                             ttATGTCTGTGGCTAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCTTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTgg                                                                          <  1:524286/150‑1 (MQ=255)
                                                                             ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTgg                                                                          >  2:5755547/1‑150 (MQ=255)
                                                                             ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTACGTgg                                                                          >  2:14560462/1‑150 (MQ=255)
                                                                               aTGTCTGTGGCTAATTTGACTCCCTTCGTTTTTTTGATTAATATTGTTTTTATTATCGTATTTAAAATTACATTAATATTTAAAATGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAg                                                                        <  1:4861093/150‑1 (MQ=255)
                                                                                 gTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACATGAAACCAAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCa                                                                      >  2:9517519/1‑150 (MQ=255)
                                                                                 gTCTGTGGCGAAATTGACTACCTTCGTGTTTTTGTTTTAGAATTTTTTTATTATAGTAATTAAAAATACATTAAAAATTAAAAAAGAAAAAGACATGAAAACCAATCACAGAATAAAAAATATAAAAAAATAAAATATTTACGTTGATCa                                                                      >  1:12817629/1‑150 (MQ=255)
                                                                                      gggCTAAATTGACTACCTTCGTTTTTTTGATTAATAATTTTTTTATTATCTTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTtta                                                                 <  1:5655642/149‑1 (MQ=255)
                                                                                          gAATTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTtattat                                                             <  1:3850892/150‑1 (MQ=255)
                                                                                           aaaTTTACTACCTTCGTTTTTTTTATTAATAATGATTTTATTATCTAAATTAAAATTAAATTAATATTAAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                            <  1:1121527/150‑1 (MQ=255)
                                                                                           aaaTTGACTACCTTCTTTTTTTTTATTAATAATGATTTTATTATCTTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                            <  1:6324728/150‑1 (MQ=255)
                                                                                           aaaTTGACTACCTTCGTTTTTTTGATTAAGATTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                            <  2:773215/150‑1 (MQ=255)
                                                                                           aaaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                            <  2:13301728/150‑1 (MQ=255)
                                                                                             aTTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCTTAATTAAAATTACATGAATATTAAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGa                                                          <  1:9768224/150‑1 (MQ=255)
                                                                                                 aCTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAATCACATAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAatat                                                      >  2:6749735/1‑150 (MQ=255)
                                                                                                     ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                                  <  2:9785813/150‑1 (MQ=255)
                                                                                                     ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACAAGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAAAATTTCCGTGGAGCATTTTATTATTGAATATagag                                                  >  2:5963765/1‑150 (MQ=255)
                                                                                                      cTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTcc                                                                              <  2:8627587/121‑1 (MQ=255)
                                                                                                         cGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGttt                                              >  2:7526341/1‑150 (MQ=255)
                                                                                                          gTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTa                                             >  2:7371553/1‑150 (MQ=255)
                                                                                                                tttaTTAATATTGATTTTATTATCTTAATTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                                       <  2:5462998/147‑1 (MQ=255)
                                                                                                                tttaTTAATAATGATTTTATTATCGTAATTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                                       <  2:10531150/147‑1 (MQ=255)
                                                                                                                ttGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                                       <  2:4696382/150‑1 (MQ=255)
                                                                                                                ttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                                       <  1:1621208/150‑1 (MQ=255)
                                                                                                                ttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                                       <  1:8606106/150‑1 (MQ=255)
                                                                                                                ttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                                       <  2:5066766/150‑1 (MQ=255)
                                                                                                                   aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa                                    >  2:6489370/1‑150 (MQ=255)
                                                                                                                    ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa                                   >  1:8927603/1‑150 (MQ=255)
                                                                                                                    ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa                                   >  1:5762049/1‑150 (MQ=255)
                                                                                                                     tAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa                                  >  2:1717741/1‑150 (MQ=255)
                                                                                                                        gAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaa                               <  2:8254977/150‑1 (MQ=255)
                                                                                                                         aaTGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAc                              <  1:9884636/150‑1 (MQ=255)
                                                                                                                           tGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATTTAGAGGTTTAACTCCGGTAAAAAACaa                            >  2:1532514/1‑150 (MQ=255)
                                                                                                                            gATTTTATTATCTTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                           <  1:14782295/150‑1 (MQ=255)
                                                                                                                            gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                           >  2:14928501/1‑150 (MQ=255)
                                                                                                                              ttttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaaga                         >  1:14570715/1‑150 (MQ=255)
                                                                                                                               tttATTATCGTAAGTAAAATTACATGATTATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                        <  2:15111639/150‑1 (MQ=255)
                                                                                                                                      tCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGa                 >  1:12365831/1‑150 (MQ=255)
                                                                                                                                       cGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                                        <  2:1025327/126‑1 (MQ=255)
                                                                                                                                        gtaagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTATCTCCGGTAAAAAACAAAGAAGCATTGAAt               >  1:6014254/1‑150 (MQ=255)
                                                                                                                                         taagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCCTTGAATg              >  2:15465480/1‑150 (MQ=255)
                                                                                                                                         taagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATg              <  1:1040477/150‑1 (MQ=255)
                                                                                                                                           attaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCa            <  1:9280250/148‑1 (MQ=255)
                                                                                                                                            ttaaAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg           <  1:6866380/149‑1 (MQ=255)
                                                                                                                                            ttaaAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg           <  1:8795048/149‑1 (MQ=255)
                                                                                                                                            gtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg           <  2:2256869/150‑1 (MQ=255)
                                                                                                                                            gtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg           >  2:9304324/1‑150 (MQ=255)
                                                                                                                                            gtaaAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg           <  1:1992730/150‑1 (MQ=255)
                                                                                                                                             taaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAgg          >  1:2687207/1‑150 (MQ=255)
                                                                                                                                               aaaTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGa        <  1:3003889/150‑1 (MQ=255)
                                                                                                                                                   tACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaaaa    <  1:10025902/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATA  >  NC_000913/238383‑238681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: