Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,491,702 A→C 10.6% intergenic (‑82/+23) yhfG ← / ← ppiA putative antitoxin for Fic/peptidyl‑prolyl cis‑trans isomerase A (rotamase A)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,491,7020AC10.6% 1974.1 / 52.1 720intergenic (‑82/+23)yhfG/ppiAputative antitoxin for Fic/peptidyl‑prolyl cis‑trans isomerase A (rotamase A)
Reads supporting (aligned to +/- strand):  ref base A (225/397);  new base C (85/2);  total (319/401)
Fisher's exact test for biased strand distribution p-value = 2.51e-31
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 749 total aligned reads displayed.

GCCTGAAAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCA  >  NC_000913/3491553‑3491851
                                                                                                                                                     |                                                                                                                                                     
 ccTGAAAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCaa                                                                                                                                                      <  1:13627870/150‑1 (MQ=255)
  cTGAAAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAg                                                                                                                                                     <  2:5290348/150‑1 (MQ=255)
   tGAAAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAATTCCGGGTTACGCCGGGAGAGCAGAAGCGGGGGCCAgt                                                                                                                                                    >  1:8492964/1‑149 (MQ=255)
   tGAAAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCaatc                                                                                                                                                    <  2:198593/150‑3 (MQ=255)
     aaaaTTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGAtt                                                                                                                                                  <  2:1145072/150‑1 (MQ=255)
          tacgattacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCcg                                                                                                                                             >  1:6365863/1‑150 (MQ=255)
            cgattacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAATCTTGCCcgcg                                                                                                                                           <  2:10140881/150‑1 (MQ=255)
             gattacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCcgcgc                                                                                                                                          >  1:14766206/1‑150 (MQ=255)
             gattacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAACGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCcgcgc                                                                                                                                          >  2:1097987/1‑150 (MQ=255)
                tacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCCAGCTTGCCcgcgcgcg                                                                                                                                       >  1:6640511/1‑148 (MQ=255)
                  cgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCCAGCTTTCCCGCGCCAGaa                                                                                                                                     >  2:4118414/1‑150 (MQ=255)
                   gCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCCCCAGTGCCcccgcggggca                                                                                                                                    >  2:7163616/1‑145 (MQ=255)
                    cTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGGGGGCCAGATTGCCCCCCCGCGAAAt                                                                                                                                   >  2:5653015/1‑150 (MQ=255)
                      gAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAAtaa                                                                                                                                 >  2:15051408/1‑148 (MQ=255)
                       aaGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCAt                                                                                                                                <  2:12924277/150‑1 (MQ=255)
                         gCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTa                                                                                                                              <  2:8761514/150‑1 (MQ=255)
                           tCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACg                                                                                                                            <  2:8158140/150‑1 (MQ=255)
                             ccAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGAATGCCCGCGCGCGAAATCATTAAGGc                                                                                                                          >  1:13022650/1‑150 (MQ=255)
                              cAGAGACGGTACTTTTGCTTATGGGTGTGTTTCTTCACTTGATGCCTCCCTGAACGTGTCAATTTGCCACAAGTATAAGCTGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCa                                                                                                                         <  1:6013522/150‑1 (MQ=255)
                              cAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCa                                                                                                                         <  2:1201322/150‑1 (MQ=255)
                                   aCGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGGCAGATTGCCCCCGCGAGAAATCATTAAGGCCGGCCt                                                                                                                    >  2:5909747/1‑150 (MQ=255)
                                   aCGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAAAAAAAACGGCAGGACt                                                                                                                    >  2:7798144/1‑150 (MQ=255)
                                     gggACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGGCAGATTTGCCCCGCGAGACAACAATAAGGCacgccaat                                                                                                                  >  1:14367641/1‑143 (MQ=255)
                                     gggACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCCCGATTGCCCGCGCGCGAAATCAATAAGGCggggcgtt                                                                                                                  >  1:4089464/1‑145 (MQ=255)
                                     gggACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACttt                                                                                                                  >  2:11720335/1‑150 (MQ=255)
                                        aCTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGc                                                                                                               <  2:12555366/150‑1 (MQ=255)
                                          ttttGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCCAGGTTGGCCCCGCGCGAAATAAATAAGGCAGGACATTTGCgg                                                                                                             >  2:1816367/1‑150 (MQ=255)
                                          ttttGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACATTAGCgg                                                                                                             >  2:15289015/1‑150 (MQ=255)
                                          ttttGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAACAATTCCCCCGCGAGAAAAAAATAAGGCAGGACATTAGCgg                                                                                                             >  1:11366171/1‑150 (MQ=255)
                                               cTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCTGGAGAGCAGAAGCGCGGGCCAGATTGGCCCCGCGAGAAAACAATAAGGCAGGCCGTTAGCGGGAAgg                                                                                                        >  2:14413299/1‑150 (MQ=255)
                                               cTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCCACAATTCCCCCCCGAGAAAACAATACGGCAGGACATTAGCCGaaaag                                                                                                        >  2:11127431/1‑148 (MQ=255)
                                                ttATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGa                                                                                                       <  1:12279630/150‑1 (MQ=255)
                                                ttATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGa                                                                                                       <  2:15505148/150‑1 (MQ=255)
                                                 tATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGAt                                                                                                      <  1:3343817/150‑1 (MQ=255)
                                                 tATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAACATTGCCCCCCCGCGGAAAAAATACAGCAGGACATTATCGGAAAGGAt                                                                                                      >  1:14996037/1‑150 (MQ=255)
                                                       tGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCCAGATTGGCCCCGCGAGAAATCAATAAGGCACGACGTTAGCGGAAAGGGTaacaac                                                                                                >  1:13507673/1‑150 (MQ=255)
                                                         aGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCg                                                                                              <  2:3602437/150‑1 (MQ=255)
                                                          gTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCgg                                                                                             <  1:9506884/150‑1 (MQ=255)
                                                           tttCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTTCCCGCGCGAGAAAACAATAAGGCAGGACTTTATCGGAAATGATAACAAACGGt                                                                                            >  1:2332022/1‑150 (MQ=255)
                                                           tttCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAAAATTCCCCCGCCAGAAATAAATAAGGCACGACATTAGCGGAAAGGATAACTaaccgt                                                                                            >  2:4601893/1‑145 (MQ=255)
                                                              cttcACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCCAGAGTGCCCGCGCGAGAAAACAATACGGCCGGACTTTTGCGGTAAGGATTACTAACGGgttt                                                                                         >  1:8199181/1‑150 (MQ=255)
                                                               ttcACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTg                                                                                        <  1:2840527/150‑1 (MQ=255)
                                                               ttcACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCCCGAGAAATCAATACCGCCGGACTTTATCGGGAAGGATAAATACAGGttttt                                                                                        >  2:13569204/1‑149 (MQ=255)
                                                                tcACTTGATGCCTCCCTGACCGGGTCCATTGGCCACACGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGa                                                                                       <  1:5571599/150‑1 (MQ=255)
                                                                tcACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGGGGGCAAGAAAGCCCCCCCGGGCAAAAAATAAAGCAGGACGATAGCGGAAAGGATAAAAACCCGGTTTgt                                                                                       >  1:15391103/1‑149 (MQ=255)
                                                                tcACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGa                                                                                       <  1:5807850/150‑1 (MQ=255)
                                                                 cACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGAc                                                                                      <  2:13901426/150‑1 (MQ=255)
                                                                  aCTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGGGGGCCAGATTGGCCGCGCGGGAAATAAATAAGGCAGGACAGTTTAGGGAAGGATAACGAACGGgtttttcg                                                                                     >  1:3817260/1‑146 (MQ=255)
                                                                  aCTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACg                                                                                     <  2:2935830/150‑1 (MQ=255)
                                                                  aCTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACg                                                                                     <  2:8541904/150‑1 (MQ=255)
                                                                   cTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACgg                                                                                    >  2:10369074/1‑150 (MQ=255)
                                                                    ttGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGc                                                                                   <  1:440306/150‑1 (MQ=255)
                                                                    ttGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCCGGCAACAATTCCCCCCCGCGGAATCAATAACGCAGGCCGGTATCGGAAACGATAAAAAACGGTTTTGAAGGc                                                                                   >  2:13272415/1‑150 (MQ=255)
                                                                     tGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTTCGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGca                                                                                  <  2:6215658/150‑1 (MQ=255)
                                                                           cTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCCCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGATGGCACATTCt                                                                            >  2:10515835/1‑150 (MQ=255)
                                                                            tCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCCAGAGTGCCCCCCCGAGAAATCAATAAGGCACGCCGTGAGCGGAAAGGAGAACAACCCGTTTTGTCGGCACAATCTg                                                                           >  2:13635089/1‑150 (MQ=255)
                                                                            tCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTg                                                                           <  1:4767823/150‑1 (MQ=255)
                                                                            tCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTg                                                                           <  2:14766206/150‑1 (MQ=255)
                                                                             cccTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTgg                                                                          <  1:12619343/150‑1 (MQ=255)
                                                                                tGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGGGGGGAAGGTTTGCCGCGCGGGAAATAATTTAGGGAGGTGTTTATCGGTAAGGATAACTAACGGTTTTGTCGGCACAATGTGGTAc                                                                       >  2:6006722/1‑150 (MQ=255)
                                                                                 gAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGGCCGCGCGAGAAAAAAATACGGCAGGACGTTAGCGGGAAGGAAAACAACCCGTTTTTAAGGCACAATATgggaag                                                                      >  1:10361961/1‑146 (MQ=255)
                                                                                 gAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACg                                                                      <  1:1002487/150‑1 (MQ=255)
                                                                                   aCGTGTCCATTTTCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCTGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGa                                                                    <  1:502771/150‑1 (MQ=255)
                                                                                   aCGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGa                                                                    <  2:333762/150‑1 (MQ=255)
                                                                                        tCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAAc                                                               <  2:641771/150‑1 (MQ=255)
                                                                                            tttGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCa                                                           <  1:2670931/150‑1 (MQ=255)
                                                                                               gCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGGCCAACGTCATGa                                                        <  2:14108591/150‑1 (MQ=255)
                                                                                               gCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGa                                                        <  2:9286167/150‑1 (MQ=255)
                                                                                                ccACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACGGTAGCGGAAAGGATAACTACCGGGTTTGACGGCACATTCTGGTACGGACCCACGTCATGAg                                                       >  2:8980067/1‑150 (MQ=255)
                                                                                                  acaAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAACATTTCCCCCGCGAGGAAACATTAAGGCAGGACGTTAGCGGAAAGGATAACTACCGGGTTTTACGGCACAATCTGGTACGGACCAACGGCATGAGTc                                                     >  2:12296471/1‑150 (MQ=255)
                                                                                                    aaGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTTCCCGCGCGAGAAATAATTACGGCCGGACTTTAGCGGAAAGGATAACAACCGGTTTTGACGGCACATTATGGTACGGACCAACGTCATGAGTCgg                                                   >  2:3718266/1‑150 (MQ=255)
                                                                                                       tataAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCAc                                                <  1:7909382/150‑1 (MQ=255)
                                                                                                         taAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCt                                              <  1:2115087/150‑1 (MQ=255)
                                                                                                              ggCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGaa                                         <  2:9533267/150‑1 (MQ=255)
                                                                                                              ggCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGaa                                         <  1:13824503/150‑1 (MQ=255)
                                                                                                                cAAATCCGGGTTACGCCGGGAGAGCAGAAGCGGGGGCCAGATTGGCCCCCCGGGAAAACAAAAAGGCAGGGCATTATCGGAAAAGGTAACAAACCGTTTTTACGGCACAATAATGTACGGACCCACCTCATGAGTCCGCCCCGGGGaaaa                                       >  2:6939924/1‑149 (MQ=255)
                                                                                                                 aaaTCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATc                                      <  2:48335/150‑1 (MQ=255)
                                                                                                                   aTCCGGGTTACGCCGGGAGAGCAGAAGCGGGGGGAAGATTTGCCGCGCGAGTAATAATTACGGCAGGTGTTTATGGGTAAGGATAAATAACGGTTTTTTAGGGAAATTATTGTAAGGTACAAAGTCATTATTGGGGACCTGGGAAATAtt                                    >  2:2223420/1‑150 (MQ=255)
                                                                                                                     ccGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTAt                                  <  2:1269984/150‑1 (MQ=255)
                                                                                                                      cGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATc                                 <  1:9344985/150‑1 (MQ=255)
                                                                                                                      cGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATc                                 <  1:3653965/150‑1 (MQ=255)
                                                                                                                       gggTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTTCCCGCGCGAGAAATAATTAACGCAGGACTTTAGCGGAAAGGATAACAACCCGTTTTTAAGGCACATTATGGTAAGGCCCAACCTCATGAGTCGGCACCGGGGGAATCTTATCg                                >  2:7677347/1‑150 (MQ=255)
                                                                                                                       gggTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCg                                <  2:9681236/150‑1 (MQ=255)
                                                                                                                        ggTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCgg                               <  1:1771121/150‑1 (MQ=255)
                                                                                                                        ggTGACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAACTCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACAGTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCgg                               <  2:2654414/150‑1 (MQ=255)
                                                                                                                         gTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGc                              <  1:711909/150‑1 (MQ=255)
                                                                                                                           tACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCaa                            <  1:6890494/150‑1 (MQ=255)
                                                                                                                               ccGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTc                        <  1:9643765/150‑1 (MQ=255)
                                                                                                                                       gCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAAACATTACGGCCGGACTTTAGCGGAAAGGATAACTACCGGTTTTGAAGGCACAATCTGGTACGGACCAACGTCATGAGTCCGCACCTGGGAAATCTTATCGGCAACGTCCCTGCCtt                >  2:8068108/1‑150 (MQ=255)
                                                                                                                                         aGACGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGTAAATCTTATCGGCAACGTCCATGCCTTTc              <  1:7290871/150‑1 (MQ=255)
                                                                                                                                             gcgcGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATTATTCGGCACCTGGGAAATCATATCGGCAACCTCCAAGCCATTcacca          >  1:10995083/1‑150 (MQ=255)
                                                                                                                                             gcgcGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTcacca          <  1:10520652/150‑1 (MQ=255)
                                                                                                                                             gcgcGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTcacca          >  1:9988338/1‑150 (MQ=255)
                                                                                                                                             gcgcGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACAACCGGTTTTGACGGCACAATCTGGTACGGACCAACGTCATGAGTCGGCCCCTGGGAAAAATTATCGGCAACGTCCATGCCATTaacca          >  1:8928209/1‑150 (MQ=255)
                                                                                                                                              cgcgGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTcaccac         >  2:2420863/1‑150 (MQ=255)
                                                                                                                                                  ggCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTa     <  1:6136671/150‑1 (MQ=255)
                                                                                                                                                   gCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTAc    >  2:10893638/1‑150 (MQ=255)
                                                                                                                                                   gCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTAc    >  1:11204810/1‑150 (MQ=255)
                                                                                                                                                   gCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTAc    >  2:3081167/1‑150 (MQ=255)
                                                                                                                                                    cAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTAcc   >  1:11776753/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCTGAAAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCA  >  NC_000913/3491553‑3491851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: