Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,504,875 T→A 11.4% intergenic (+92/+60) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,504,8750TA11.4% 1754.1 / 57.2 639intergenic (+92/+60)frlR/yhfSputative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base T (167/371);  new base A (80/2);  total (258/381)
Fisher's exact test for biased strand distribution p-value = 1.87e-33
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 708 total aligned reads displayed.

CGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGA  >  NC_000913/3504726‑3505024
                                                                                                                                                     |                                                                                                                                                     
cGAACTCTTCTGCCGCGCCAAGCGCATCACCTTAGCTATTGATAATAAAAGACCTTAACCGGCGGCCGGAGAAGATGCGCCCGCATCGCATCCGGCGATGCTGGCGCGGTGAATGGTCCTTCCCGTTCGTGCCCCTCGCCCGAACCgtct                                                                                                                                                       >  2:12955848/1‑150 (MQ=255)
   aCTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTccc                                                                                                                                                    >  1:4112103/1‑150 (MQ=255)
     tcttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCGAAACCACAccccc                                                                                                                                                  >  1:14126388/1‑149 (MQ=39)
     tcttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCa                                                                                                                                                  <  1:8597948/150‑1 (MQ=255)
       ttctGCCGCGCCACTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaca                                                                                                                                                <  1:6497638/150‑3 (MQ=255)
        tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAg                                                                                                                                               <  2:8947879/150‑1 (MQ=255)
         ctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACACCCCCAAgg                                                                                                                                              >  1:12831939/1‑150 (MQ=39)
          tGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAggg                                                                                                                                             <  2:4582295/150‑1 (MQ=255)
           gCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCTAACCCTCACCCCAAAgggg                                                                                                                                            >  2:15246251/1‑150 (MQ=255)
              gcgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCCCACCCAAACCCACAACCCCAAGGGGCgg                                                                                                                                         >  1:6194248/1‑149 (MQ=38)
                gcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAgg                                                                                                                                       <  2:1463455/150‑1 (MQ=255)
                gcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAgg                                                                                                                                       <  2:826700/150‑1 (MQ=255)
                     tCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAgggttag                                                                                                                                  <  1:4345790/150‑5 (MQ=38)
                        cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCTCGCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                                               >  1:14019643/1‑150 (MQ=255)
                          tCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCa                                                                                                                             <  1:3566465/150‑1 (MQ=255)
                             ccTTAACTATTGATCATAAACGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                                          <  1:6061856/150‑1 (MQ=255)
                             ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGAGAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCTAACCCACACCCCCAAGGGGCGAGGGGACCGTTCAGTa                                                                                                                          >  2:8439291/1‑150 (MQ=39)
                              cTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                                         <  1:11503671/150‑1 (MQ=255)
                                tAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACgg                                                                                                                       <  2:5839704/150‑1 (MQ=255)
                                  aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGaa                                                                                                                     <  2:7704517/150‑1 (MQ=255)
                                  aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGaa                                                                                                                     <  1:2941677/150‑1 (MQ=255)
                                    tATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAtt                                                                                                                   >  2:10451626/1‑150 (MQ=255)
                                    tATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAtt                                                                                                                   <  1:8834184/150‑1 (MQ=255)
                                     aTTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCCCCCGAACCCACTCCCCAAAGGGGCGAGGGGACCGTTACGTACGGAAttt                                                                                                                  >  2:13899835/1‑150 (MQ=39)
                                     aTTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCGAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTAAGGAAatt                                                                                                                  >  2:2470876/1‑150 (MQ=255)
                                      ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTa                                                                                                                 <  1:13750693/150‑1 (MQ=255)
                                       tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAg                                                                                                                <  2:15575702/150‑1 (MQ=255)
                                         ataataAAAGACATTTACCGTTGGCCGGATAAGATGCGCCAGCTTCGCATGCGGCGATGGTGGCGCGTTGAATTTTTCATCCCGTACGTTCCCCTCTCGCTTAGCCTCTCCCCTATGGGGCGTGGGGACGGTTCAGTACGGTATTTTGCt                                                                                                              >  2:15480765/1‑150 (MQ=255)
                                         ataataAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCt                                                                                                              <  2:2870407/150‑1 (MQ=255)
                                           aataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACTCCCCAAAGGGGCGGGGGGACCGGTCAGTACGGAATTTTGCGTg                                                                                                            >  1:15285480/1‑150 (MQ=38)
                                            ataaAAGACCGTAACCGTAGGCCGGATAAGATGCGCCAGCCTCGCATCCGGCGATGCTGGCGCGTTGAATTTTACCTCCCGTACGGTCCCCTCCCCCTACCCCTCTCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                                           <  2:4796865/150‑1 (MQ=38)
                                              aaaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACg                                                                                                         <  1:9370866/150‑1 (MQ=255)
                                               aaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACACCCCCAACGGGCGAGGGGCCCGGTAAGTACCGAAATTATCTTGACgg                                                                                                        >  2:10666090/1‑150 (MQ=37)
                                                aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTCACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGt                                                                                                       <  2:9466504/150‑1 (MQ=255)
                                                 aGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAAACCACACCCCAAAAGGGCCAGGGGACCGTTAAGTACGGAATTTAGCATTACGGTg                                                                                                      >  2:3261064/1‑150 (MQ=38)
                                                     aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAATTAGCTTGACGGTGCTAt                                                                                                  >  2:10329645/1‑150 (MQ=255)
                                                     aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGAACGTTCCCCACAACCCAAACCAAACCCCAAAAGGGCGCGGGGGCCGGTCCCTACCGAAAATTGCTTTACGGGGCTag                                                                                                  >  1:184190/1‑149 (MQ=19)
                                                     aTTAACCGAAGGCCGGATAAGATGCGCCAGCAACGCATCCGGCGTTGCTGGCGCGTTGTATTTTACATCCCGTACGTTCCCCACAACCTAAACCACACCCCAAAGGGGGGAGGGGACCGTGCGGATAGCAATTTAGCATGACGGGGCttt                                                                                                  >  1:9092771/1‑148 (MQ=255)
                                                      ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                                                 >  2:14080348/1‑150 (MQ=255)
                                                       tAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCACTCACCCAAAACCACAACCCAAACGGGCGAGGGGACCCGTCAGTAAGGAAATTATCATGACGGTGCGAtac                                                                                                >  2:6176257/1‑148 (MQ=25)
                                                        aaCCGTCGGCCGGAGAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                               <  2:1182159/150‑1 (MQ=255)
                                                         aCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAg                                                                                              <  1:6787149/150‑1 (MQ=255)
                                                          ccGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCAGTACCGAAATTAGCTTGACGGTGCTATCCAGc                                                                                             >  2:12614079/1‑150 (MQ=255)
                                                            gTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGGACGGTGCCCGCCCCCAAAACCACAACCCAAACGGGCGAGGGGACCCGTCAATAAGGGAATTAGCGTGTCGGGGCGATACaccca                                                                                           >  1:8194058/1‑146 (MQ=16)
                                                                gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTaaa                                                                                       <  1:5616753/150‑1 (MQ=255)
                                                                 ccGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAAt                                                                                      <  1:953239/150‑1 (MQ=255)
                                                                   ggATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAc                                                                                    <  1:2586714/150‑1 (MQ=255)
                                                                    gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACt                                                                                   <  1:14479547/150‑1 (MQ=255)
                                                                      tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                                                 <  2:731016/150‑1 (MQ=255)
                                                                      tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                                                 <  1:5160227/150‑1 (MQ=255)
                                                                         gATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAACCGAAACCACAACCCAAAGGGGCGAGGGGGCCCTTCAGTACGGAATTTAGCATGACGGTGCTATACAGCATTAAATAATGGcca                                                                              >  2:219849/1‑148 (MQ=255)
                                                                            gcgtCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCACTCCCCCAAGGGGCGCGGGGACCGGTCCGGACGGGATGTAGCGGGACGGTGCTATCCAGCCTTAAATAATGGcccaac                                                                           >  2:12177575/1‑145 (MQ=255)
                                                                             cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCACCCAAAACCACACCCCAAAGGGGCGGGGGGGCCGTTCAGTAAGGGATATTTCTTTACGGTGCGAACCAGCAGTAAAAACTGGCAAAAct                                                                          >  2:6793921/1‑150 (MQ=255)
                                                                              gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAgg                                                                                                                                       >  2:13688753/1‑88 (MQ=255)
                                                                                cAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACtctc                                                                       <  2:5526031/150‑1 (MQ=255)
                                                                                cAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACCCCCAAACCCACAACCCCAAAGGGCGAGGGGGCCGGTCAGTACGGAATTTATCATTAAGGTGCTATCCACCAATAAAAAATGGGaaaaaacac                                                                       >  1:15041545/1‑143 (MQ=255)
                                                                                   catcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGTCCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACg                                                                    <  2:4112103/150‑1 (MQ=255)
                                                                                       gcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                                                <  2:653158/150‑1 (MQ=255)
                                                                                         atcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAAt                                                              <  1:4673086/150‑1 (MQ=255)
                                                                                             ggCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGATTTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgc                                                          <  1:11947162/150‑1 (MQ=255)
                                                                                             ggCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgc                                                          <  2:14827867/150‑1 (MQ=255)
                                                                                                 aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCTAAACCACTCCCCCAAGGGGCGAGGGGGCCGGTCACGACGGGAATTAGCGTGACGGTGCGATCCCGCCTTAAATACGGGCAATACTAACACGCAAAAACCgcagca                                                      >  2:13586241/1‑150 (MQ=255)
                                                                                                   gCTGGCGCGTTGAATTTTCCATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCACCGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAcc                                                    <  1:1125528/150‑1 (MQ=255)
                                                                                                     tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTACCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACATGCAAAACTCTCACGCAAAAAACGCAGCACCGt                                                  >  2:8584378/1‑150 (MQ=38)
                                                                                                     tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACAAACTAAACCACTACCCAAAGGGGCGAGGGGACCGTTCAGTAACGAATTTAGCTTGACGGTGCTATACAGCAGTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGt                                                  >  2:8854413/1‑150 (MQ=255)
                                                                                                      ggCGCGTTGAATTTTACCTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGtc                                                 <  1:10026267/150‑1 (MQ=255)
                                                                                                      ggCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGtc                                                 <  2:11578937/150‑1 (MQ=255)
                                                                                                             tGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCAc                                          <  1:12149861/150‑1 (MQ=255)
                                                                                                             tGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCAc                                          <  1:793451/150‑1 (MQ=255)
                                                                                                                 ttttACATCCCGTACGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTAACGCAAAATCCGCCGCACCGTCTCTTCACCGcg                                      >  2:1861198/1‑149 (MQ=255)
                                                                                                                  tttACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTg                                     <  1:7440069/150‑1 (MQ=255)
                                                                                                                   ttACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGc                                    <  1:10451921/150‑1 (MQ=255)
                                                                                                                       aTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCGCCGCTGCGAtt                                <  2:10856696/150‑1 (MQ=255)
                                                                                                                        tCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTc                               <  1:3163514/150‑1 (MQ=255)
                                                                                                                        tCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTc                               <  1:14755758/150‑1 (MQ=255)
                                                                                                                         cccGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCg                              <  2:6606290/150‑1 (MQ=255)
                                                                                                                         cccGTACGTTCCCCTAAACCTAAACCTATACCCAAATGGGCGAGGGGACCGTTAAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCAACGCTGCGATTCg                              >  2:2307394/1‑150 (MQ=255)
                                                                                                                          ccGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCgg                             <  2:564698/150‑1 (MQ=255)
                                                                                                                                 ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATg                      >  2:3411761/1‑150 (MQ=255)
                                                                                                                                 ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATg                      >  2:15025446/1‑150 (MQ=255)
                                                                                                                                 ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATg                      >  2:5195475/1‑150 (MQ=255)
                                                                                                                                    cccTCACCCTACCCCTCTCCCCACCGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGCCGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa                   <  2:7903857/150‑1 (MQ=255)
                                                                                                                                     ccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGaa                  >  1:2318933/1‑150 (MQ=255)
                                                                                                                                      cTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACg                                                                    <  2:6063369/99‑1 (MQ=255)
                                                                                                                                      cTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAAt                 >  2:3807061/1‑150 (MQ=255)
                                                                                                                                           ccgcccccTCTCCCCCCCGGGGCGCGGGGACCGTTCAGTCCGGAAGTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAACTCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac            <  1:13420312/145‑1 (MQ=255)
                                                                                                                                           ccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac            >  1:3403633/1‑150 (MQ=255)
                                                                                                                                           ccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac            >  1:10738263/1‑150 (MQ=255)
                                                                                                                                           ccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac            >  2:9273905/1‑150 (MQ=255)
                                                                                                                                              aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAAt         <  2:13192818/150‑1 (MQ=255)
                                                                                                                                                 ccTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGtt      >  1:3844225/1‑150 (MQ=255)
                                                                                                                                                  ctctCCGCAACGGGGCGAGGGGACCGGTCAGTTCGGTATTGAGCTTGACGGTGCTATCCAGCATTTAATACTGGCAATACTCTCACGCAATATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTACTGCGAATCGCACATTGTTc     <  2:10060966/150‑1 (MQ=255)
                                                                                                                                                  ctctCCCCAAAGGGGCGAGGGGACCGTTCAGTCCGGCATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGACTCGCACAATGTTc     >  2:11344042/1‑150 (MQ=255)
                                                                                                                                                  ctctCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTCATGCGAATCGCACAATGTTc     >  2:3625691/1‑150 (MQ=255)
                                                                                                                                                  ctctCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTc     <  1:5369030/150‑1 (MQ=255)
                                                                                                                                                   tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                                      <  2:14495375/52‑1 (MQ=255)
                                                                                                                                                   tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCt    >  1:1307600/1‑150 (MQ=255)
                                                                                                                                                     tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAGTTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGa  <  1:15486075/150‑1 (MQ=255)
                                                                                                                                                     tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGa  >  1:12510101/1‑150 (MQ=255)
                                                                                                                                                     tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGa  >  2:9714137/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGA  >  NC_000913/3504726‑3505024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: