Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,501 T→A 11.6% intergenic (+56/+31) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,5010TA11.6% 1869.2 / 61.7 654intergenic (+56/+31)ilvC/ppiCketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
Reads supporting (aligned to +/- strand):  ref base T (165/380);  new base A (80/4);  total (263/391)
Fisher's exact test for biased strand distribution p-value = 7.29e-32
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 671 total aligned reads displayed.

GAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCT  >  NC_000913/3959352‑3959650
                                                                                                                                                     |                                                                                                                                                     
 aaCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATCTGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACCGGGAGAGGGAGAAAACACTc                                                                                                                                                      >  2:7328044/1‑150 (MQ=255)
 aaCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTc                                                                                                                                                      <  1:6912124/150‑1 (MQ=255)
   cGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCaa                                                                                                                                                    <  2:1290616/150‑1 (MQ=255)
    gAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACACAAg                                                                                                                                                   >  1:3591924/1‑150 (MQ=255)
    gAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACACCCACACGGGGAGGGGGAAAAAACACAAg                                                                                                                                                   >  2:4433333/1‑150 (MQ=255)
     aaGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAgg                                                                                                                                                  <  2:12428696/150‑1 (MQ=255)
         gATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCtt                                                                                                                                              <  2:4129187/150‑1 (MQ=255)
            tCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCCCCCCCACCCACACCCCCAGGGGGAGGGGGAAAACACACAAGGCCAtctc                                                                                                                                           >  1:4918559/1‑150 (MQ=255)
             cGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTcc                                                                                                                                          <  2:10899527/150‑1 (MQ=255)
               cAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACCGGGAGAGGGAGAAAAAACTCAAAGCCATCAccgg                                                                                                                                        >  2:1177092/1‑148 (MQ=38)
               cAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTg                                                                                                                                        <  2:13585879/150‑1 (MQ=255)
               cAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTg                                                                                                                                        <  1:6460851/150‑1 (MQ=255)
                 gCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGga                                                                                                                                      <  1:13128161/150‑1 (MQ=255)
                 gCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCgggc                                                                                                                                      >  2:8829164/1‑149 (MQ=255)
                   cATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGgaga                                                                                                                                    <  2:12013072/150‑1 (MQ=255)
                    aTGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGaa                                                                                                                                   <  1:1752929/150‑1 (MQ=255)
                     tGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGACCCGCTCCCCCAGGGGGTGGGGGTAAACAATCAAGGCCGTATCCGGGAGAAg                                                                                                                                  >  1:4774930/1‑150 (MQ=255)
                      gCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAgg                                                                                                                                 <  1:11736591/150‑1 (MQ=255)
                        gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCCACACACACCCACACGGGGCGGGCGAAAAAAAACAAGGCCCTCTCCAGGCGGAGGcc                                                                                                                               >  2:5835631/1‑150 (MQ=255)
                             aGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCt                                                                                                                          <  2:1132365/150‑1 (MQ=255)
                               cAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTAt                                                                                                                        <  2:13009619/150‑1 (MQ=255)
                                aggtaggtAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTAtt                                                                                                                       <  1:14798186/150‑1 (MQ=255)
                                 ggtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTTTTa                                                                                                                      <  1:2007677/150‑1 (MQ=255)
                                 ggtagggaAGCAACTGCGCGGCGATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATTCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACGCAAGGCCTTCTCCAGGAGAAGGCCTTgagtgtt                                                                                                                      <  1:4124603/150‑7 (MQ=37)
                                  gtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCAAACCCCCACCCACACCCCCACGGAGAGGGCGAAAAAAAAAAAGGCCGAATCCGGGCGAAGGCCATGCAATTat                                                                                                                     >  2:5579954/1‑149 (MQ=255)
                                  gtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGGCCGCCCCCCCACCCACTCCCCCCCGGGGCGGGCGAAAAACAACAAGGCCGTGACCAGGTGAAGGCCGTGCGATTat                                                                                                                     >  1:452258/1‑149 (MQ=255)
                                     ggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTg                                                                                                                  <  1:14480014/150‑1 (MQ=255)
                                       taAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGGTCAGTGCGCGCTGAGGCCCGCACCCCCACCCACTCCCCCCCGGCGCGGGCCAAAACACACAAGGCCCTCACCGGGCGGCGGCCCTGCTCTTAGGTGCg                                                                                                                >  2:9678264/1‑150 (MQ=255)
                                        aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCCACCCACACCCACAGGGGGATGGAGAAAAAACACAATGCCATAACCAGGAGAATGGCTTGCGAATATTTTCgg                                                                                                               >  1:9085696/1‑150 (MQ=255)
                                        aaGAAACTGATAGGCTATATGAATTATATGAAACGTATTGCTGTTGCGGGTTAAGTGCTCGCTGATGCCCTCACCCCGACCCTCTCCCACCGGGAGAGGTATAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCgg                                                                                                               <  1:14036745/150‑1 (MQ=38)
                                          gAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTa                                                                                                             <  2:13870610/150‑1 (MQ=255)
                                          gAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTa                                                                                                             <  1:11410781/150‑1 (MQ=255)
                                           aaaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCAAAAACACAGGGAGAGGGAGAAAACAAAAAAGGCCATCAACGGGGGAAGGCCATGGGATTAGtttggggac                                                                                                            >  1:12182695/1‑143 (MQ=255)
                                             aCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCAAAACCACCCGGGGAGGGGGAAAAAACACAAAGCCTTCTACAGGATCACGCCATTGTATTTTTTGCGGGACAg                                                                                                          >  2:14531973/1‑150 (MQ=255)
                                              cTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc                                                                                                         <  1:7151059/150‑1 (MQ=255)
                                                  gcgGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCCACCCACACCCACACGGAGAGGGAGGAAACACACAATGCCATCTCCCGGGGAAGGCCGTGCTATTAGTTTCGGTGAAGCAcca                                                                                                     >  2:1771439/1‑149 (MQ=255)
                                                     gCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGGCCGCACCCCCACCCAAAACCCCAGGGGGAGTGGGAAAAAACACAAAGGCCTGACCGGGCGAATGCCGTGCGATTATTTTAGGTGCAGCAccggaa                                                                                                  >  1:10483113/1‑146 (MQ=255)
                                                      cTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                                                                                                 <  1:5724275/150‑1 (MQ=255)
                                                         tatGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGTAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAattat                                                                                              <  1:3373418/150‑1 (MQ=255)
                                                         tatGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatgat                                                                                              <  1:15136731/150‑1 (MQ=255)
                                                          atGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCTAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatgatg                                                                                             <  1:5308504/150‑1 (MQ=255)
                                                           tGACAGATATGCAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCGACCCTCGCCCACCGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCGGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgt                                                                                            <  2:1324314/150‑1 (MQ=255)
                                                           tGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgt                                                                                            <  1:7561898/150‑1 (MQ=255)
                                                           tGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgt                                                                                            <  2:10682206/150‑1 (MQ=255)
                                                             aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                                                                          <  2:12748923/150‑1 (MQ=255)
                                                               aGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTtc                                                                                                                                             <  1:12836332/97‑1 (MQ=255)
                                                               aGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACAACCACAGGGGGGGGGGGAAAACACACAATGCCATCAACTGGGGAATGCCTTGCTATTAGTTGCGGTACAGCAACATAATTAtgttgta                                                                                        >  1:6262149/1‑146 (MQ=255)
                                                                 atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc                                                                                      <  2:8990339/150‑1 (MQ=255)
                                                                     gAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGaa                                                                                  <  2:4918559/150‑1 (MQ=255)
                                                                      aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAAc                                                                                 <  2:3256176/150‑1 (MQ=255)
                                                                         cGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                                                              <  1:6096587/150‑1 (MQ=255)
                                                                         cGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                                                              <  1:11178002/150‑1 (MQ=255)
                                                                         cGTATTGCTGTTGCGGGTGAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                                                              <  2:8210113/150‑1 (MQ=255)
                                                                              tGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGtgtg                                                                         <  2:13977949/150‑1 (MQ=255)
                                                                                 tGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACACCCACAGGGAGAGGGAGAAAAAACTAAAGGCCTTCTACGGGAGAAGGCCTTGCTAATATTTGCGGTACAGCACCATAAAGATGTTTTATCCGAACAGGGTTTTCAg                                                                      >  2:8431640/1‑150 (MQ=25)
                                                                                   ttGCGGGTTAAGTGCGCGCTGCTGCCCGCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTGCTCCTGGAGAAGGCCTTGCTAAGAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCg                                                                    <  1:3982137/150‑1 (MQ=38)
                                                                                   ttGCGGGTTAAGTGCGCGCTGATGCCCTCAACCCCCCCCAAACCCACAGGGCGAGGGGGAAAAAAAACAAAGCCATAAACAGGAGAAGGCCGAGCTAATATTTTCGGTAAAGCACCAAAAAGAATTGATATCCCAAAAGGGTGTGCCGcc                                                                    >  2:2758427/1‑149 (MQ=255)
                                                                                   ttGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATCAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCg                                                                    >  2:15234891/1‑150 (MQ=255)
                                                                                     gCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCggg                                                                  <  1:3153352/150‑1 (MQ=255)
                                                                                     gCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCggg                                                                  <  1:9792484/150‑1 (MQ=255)
                                                                                        ggTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCg                                                               <  1:6685011/150‑1 (MQ=255)
                                                                                          ttAAGTGCGCGCTGATGCCCACACCCCGACCCACTCCCACAGGGAGAGGGAGAAAAAACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCATAATGATGTGATAACCGAACTGGGTGTGCAGCGGGCCGGt                                                             >  2:3602865/1‑150 (MQ=39)
                                                                                           tAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTc                                                            <  1:10005159/150‑1 (MQ=255)
                                                                                           gaaGTGCGCGCGGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTc                                                            <  1:15472168/149‑1 (MQ=255)
                                                                                             aGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCgg                                                          <  2:9475722/150‑1 (MQ=255)
                                                                                              gTGCGCGCTGATGCCCTCAACCCCACCCTATCCCACAGGGGGAGGGAGAAAACACACAATGCCATCTCCTGGGGAAGGCCTTGCTATTAGTTGCGGTACAGCACCATAATGATGTGTTATCCCAACTGGGTGTGCAGCGGGCCGGTCGGc                                                         >  2:15038182/1‑150 (MQ=37)
                                                                                                gcgcgcTGATGCCCTCACCCCGACCCTCTCCCACCGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTc                                                       <  2:11151437/150‑1 (MQ=255)
                                                                                                 cgcgcTGATGCCCTCACCCCGACCCTATCCCACAGGGCGAGGGAGAAAACACACAATGCCTTAACCGGGAGAAGGCCGTGCGATTATTTGCGGTACAGCACCATAATGATGTGATATACCAACTGGGTGTGCAGCGGGCCGGTCGGCTcc                                                      >  2:6430478/1‑150 (MQ=37)
                                                                                                  gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                                     <  1:10226462/150‑1 (MQ=255)
                                                                                                  gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGCGAGAGGGAGAAAACACACAAGGCCTTCTCCTGGAGAAGGCCTAGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                                     <  1:267058/150‑1 (MQ=255)
                                                                                                         tGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGGTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCgg                                              >  2:2537242/1‑150 (MQ=255)
                                                                                                             cTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAg                                                                      >  2:1017597/1‑122 (MQ=255)
                                                                                                             cTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCg                                                           >  2:6930777/1‑133 (MQ=255)
                                                                                                             cTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACaa                                          >  2:6209164/1‑150 (MQ=255)
                                                                                                               cACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAaga                                        <  1:9603716/150‑1 (MQ=255)
                                                                                                                aCCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACAAAAGGCCATCTCCTGGAGAAGGCCTTGCTATTATTTGCGGTACAGCACCATAATTATGTGATATACGAACTGGGTGTTCATCGGGCCGGTCGGCGCCAGTACCGGACAagag                                       >  1:9996256/1‑150 (MQ=255)
                                                                                                                    cGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaaa                                   <  1:10938655/150‑1 (MQ=255)
                                                                                                                     gACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAAc                                  >  2:4360139/1‑150 (MQ=255)
                                                                                                                       cccGCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCa                                <  2:4736070/150‑1 (MQ=255)
                                                                                                                         ctctCCCTGTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTg                                                                               >  2:12358345/1‑101 (MQ=255)
                                                                                                                         ctctCCCTGTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACt                              >  2:1606244/1‑150 (MQ=255)
                                                                                                                         ctctCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaa                                     <  2:15417903/143‑1 (MQ=255)
                                                                                                                         ctctCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACt                              <  1:13673459/150‑1 (MQ=255)
                                                                                                                          tctcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACtt                             >  1:2256845/1‑150 (MQ=255)
                                                                                                                          tctcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACtt                             <  2:7547288/150‑1 (MQ=255)
                                                                                                                              ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATc                         >  2:11517664/1‑150 (MQ=255)
                                                                                                                              ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTACTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATc                         <  2:2406871/150‑1 (MQ=255)
                                                                                                                                     gagagggagaAAACACTCACGGCCGTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGcc                  <  2:10521369/150‑1 (MQ=255)
                                                                                                                                      agagggataAAACACTCACGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCg                 <  1:12583679/150‑1 (MQ=255)
                                                                                                                                       gagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCgg                <  1:8050598/150‑1 (MQ=255)
                                                                                                                                            agaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCa           >  1:12759499/1‑150 (MQ=255)
                                                                                                                                             gaAAACCCACAAGGCCTCCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCAt          <  1:847883/150‑1 (MQ=255)
                                                                                                                                                aaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGaa              <  2:5295543/143‑1 (MQ=255)
                                                                                                                                                aaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTg       >  2:8553551/1‑150 (MQ=255)
                                                                                                                                                  cacTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGAc     <  1:5046629/150‑1 (MQ=255)
                                                                                                                                                  cacTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGAc     <  1:8248427/150‑1 (MQ=255)
                                                                                                                                                     tCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGaa              >  1:2394665/1‑138 (MQ=255)
                                                                                                                                                     tCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCt  >  1:2859538/1‑150 (MQ=255)
                                                                                                                                                     tCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCt  >  2:7975853/1‑150 (MQ=255)
                                                                                                                                                     tCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCt  >  1:11888232/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCT  >  NC_000913/3959352‑3959650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: