Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 705,951 T→A 5.5% intergenic (+61/‑142) nagE → / → glnS N‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components/glutamyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913705,9510TA 5.5% 515.6 / 15.2 146intergenic (+61/‑142)nagE/glnSN‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components/glutamyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (60/77);  new base A (4/4);  total (65/81)
Fisher's exact test for biased strand distribution p-value = 7.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

Only 100 of 151 total aligned reads displayed.

CAATATCGACGATTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGA  >  NC_000913/705803‑706100
                                                                                                                                                    |                                                                                                                                                      
caATATCGACGATTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAtc                                                                                                                                                       >  2:2863574/1‑150 (MQ=255)
caATATCGACGATTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGGCGCATCCAACAATGACAAGCGGTGGAGAtc                                                                                                                                                       >  2:1613583/1‑150 (MQ=255)
     tCGACGATTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTtctc                                                                                                                                                  <  2:1932265/150‑1 (MQ=255)
      cgacgaTTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTctct                                                                                                                                                 <  2:2421235/150‑1 (MQ=255)
      cgacgaTTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTctct                                                                                                                                                 <  2:1632580/150‑1 (MQ=255)
       gacgaTTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTg                                                                                                                                                <  2:856463/150‑1 (MQ=255)
       gacgaTTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTg                                                                                                                                                <  1:996712/150‑1 (MQ=255)
        acgaTTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGc                                                                                                                                               >  2:955113/1‑150 (MQ=255)
        acgaTTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGc                                                                                                                                               >  2:2149841/1‑150 (MQ=255)
         cgaTTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGcc                                                                                                                                              <  1:2589262/150‑1 (MQ=255)
                aGTGGCTTGATCATTAAAGCTCAGGGTCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCttttt                                                                                                                                      >  1:1232391/1‑150 (MQ=255)
                    gCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCttttttttt                                                                                                                                  <  2:1861992/150‑1 (MQ=255)
                    gCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCttttttttt                                                                                                                                  <  1:353933/150‑1 (MQ=255)
                    gCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCttttttttt                                                                                                                                  >  1:883367/1‑150 (MQ=255)
                    gCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCttttttttt                                                                                                                                  <  2:2698287/150‑1 (MQ=255)
                    gCTTGAACAATAAAGCACAGGGCCATATTGTGGCGGGACAAACACCGCTCTATCAAAACAAAAACTAAACTCCTTTATGCCATATGCGACGATTGAGCGACGCATCCAACAATGACAACCGGTGCAGATCTTCTCTGCCGCttttttttt                                                                                                                                  <  2:1251668/150‑1 (MQ=255)
                        gATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTtcatc                                                                                                                              >  1:2508158/1‑150 (MQ=255)
                           cATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCatcaat                                                                                                                           <  2:1157712/150‑1 (MQ=255)
                           cATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCatcaat                                                                                                                           <  1:1736526/150‑1 (MQ=255)
                               aaaGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTCATCAATCATcc                                                                                                                      <  1:38662/150‑1 (MQ=255)
                                 aGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATccc                                                                                                                     <  1:403658/150‑1 (MQ=255)
                                   cTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATAAATAATCCCCa                                                                                                                   >  1:2012359/1‑150 (MQ=255)
                                      aGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATaa                                                                                                                >  2:269127/1‑150 (MQ=255)
                                              atTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTTATCAATCATCCCCATAATCCTTGtt                                                                                                        <  1:2751154/150‑1 (MQ=255)
                                              atTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGtt                                                                                                        <  1:2780251/150‑1 (MQ=255)
                                               ttGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGTTCTTCTCTGCCGCTTTTTTTTTCATCTATCATGCGCATAATCCTTGTTa                                                                                                       >  2:1800832/1‑150 (MQ=255)
                                               ttGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTa                                                                                                       >  1:1463099/1‑150 (MQ=255)
                                               ttGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTAATCAATCAACCCCAAAATCCTTGTTa                                                                                                       >  2:736897/1‑150 (MQ=255)
                                                 gtgGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGa                                                                                                     <  1:2863552/150‑1 (MQ=255)
                                                  tgGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGAt                                                                                                    <  1:885745/150‑1 (MQ=255)
                                                  tgGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATACTTGTTAgtt                                                                                                    >  1:1268252/1‑148 (MQ=255)
                                                             acacCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTa                                                                                         >  2:459388/1‑150 (MQ=255)
                                                               acCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTaaa                                                                                       <  1:1422371/150‑1 (MQ=255)
                                                               acCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTaaa                                                                                       >  2:2484833/1‑150 (MQ=255)
                                                               acCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTaaa                                                                                       >  2:1647182/1‑150 (MQ=255)
                                                               acCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTaaa                                                                                       >  2:1200459/1‑150 (MQ=255)
                                                               acCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTaaa                                                                                       >  1:863201/1‑150 (MQ=255)
                                                               acCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTaaa                                                                                       >  1:863280/1‑150 (MQ=255)
                                                               acCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTAATCAAAAATCCCCATAATCCTTGTTAGATTATCAATTTTaaa                                                                                       >  1:863285/1‑150 (MQ=255)
                                                                ccGCTGTATGAAATCAAAAAGTAATCTGCTTTACGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTaaaa                                                                                      <  1:638555/150‑1 (MQ=255)
                                                                  gCTGTACGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTaaaaaa                                                                                    <  2:121483/150‑1 (MQ=255)
                                                                     gTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTAAACAATCATCCCCATAAACCTTGTTGGATTATAAATTTTAAAAAACTa                                                                                 >  1:1050552/1‑150 (MQ=255)
                                                                       aTGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAAc                                                                               <  1:1720189/150‑1 (MQ=255)
                                                                       aTGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGAGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAAc                                                                               >  2:2862174/1‑150 (MQ=255)
                                                                        tGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACa                                                                              <  1:955113/150‑1 (MQ=255)
                                                                        tGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAAACCTTGTTAGATTATCAATTTTAAAAAAATAACa                                                                              >  2:2849864/1‑150 (MQ=255)
                                                                           aaTCAAAAAGTAATCTTCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGtt                                                                           <  2:2013811/150‑1 (MQ=255)
                                                                              cAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTc                                                                        <  2:2670168/150‑1 (MQ=255)
                                                                               aaaaaGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCa                                                                       >  2:292028/1‑150 (MQ=255)
                                                                                aaaaGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAg                                                                      >  2:172994/1‑150 (MQ=255)
                                                                                 aaaGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGc                                                                     >  2:2397305/1‑150 (MQ=255)
                                                                                   aGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAAACAACCCCAAAATCCATGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGcca                                                                   >  1:2034144/1‑149 (MQ=255)
                                                                                        tCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTccc                                                              <  1:658524/150‑1 (MQ=255)
                                                                                          tGCTTTATGCCTGATGCGACGCGTGAGCGTCGCATCCAACAAAGACAAGCGGTGGAGATCTTCTCTGCCGCGTTTTTTTTAATCAATCATCCCCAAAAACCATGTTAGATTATAAATTTTAAAAAAATAAAACTTGTCATCCGGTCCCGc                                                            >  1:1462165/1‑150 (MQ=255)
                                                                                           gCTTTATGCCTGATGCGACGCTTGAGCGGCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATACCCAAAATCCGTGGTAGAGTATCCATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCt                                                           >  2:2571690/1‑150 (MQ=255)
                                                                                              ttATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTta                                                         <  2:2307607/150‑1 (MQ=255)
                                                                                                aTGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATaa                                                      <  1:1200459/150‑1 (MQ=255)
                                                                                                aTGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATaa                                                      <  1:1412278/150‑1 (MQ=255)
                                                                                                  gCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGa                                                    <  1:2091787/150‑1 (MQ=255)
                                                                                                   ccTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGAt                                                   <  2:403592/150‑1 (MQ=255)
                                                                                                   ccTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGAt                                                   <  1:93638/150‑1 (MQ=255)
                                                                                                   ccTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGAt                                                   <  1:93558/150‑1 (MQ=255)
                                                                                                       aTGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATa                                               <  1:2645900/150‑1 (MQ=255)
                                                                                                       aTGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATa                                               <  2:2539572/150‑1 (MQ=255)
                                                                                                       aTGCGACGCTCAAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATa                                               >  2:1070578/1‑150 (MQ=255)
                                                                                                        tGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATa                                               <  1:102026/150‑1 (MQ=255)
                                                                                                        tGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCCGCCTGTCCCGCTTATAAGATCATAc                                              <  1:718263/150‑1 (MQ=255)
                                                                                                        tGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATAc                                              <  1:1184826/150‑1 (MQ=255)
                                                                                                        tGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATAc                                              <  2:111755/150‑1 (MQ=255)
                                                                                                        tGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATAc                                              <  2:1315287/150‑1 (MQ=255)
                                                                                                         gCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACg                                             >  2:1108053/1‑150 (MQ=255)
                                                                                                          cGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGc                                            >  2:1941511/1‑150 (MQ=255)
                                                                                                          cGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTAAGCCTGTCCCGCTTATAAGATCATACGc                                            >  2:1691967/1‑150 (MQ=255)
                                                                                                           gACGCTTGAGCGTCGCAGCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTAGTTAGATGATCAATTTTAAAAAACTAACAGGTGTCAGCCTGTCCCGCGTAGCAGATCATACGcc                                           >  2:1596033/1‑150 (MQ=255)
                                                                                                           gACGCGTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTAGCAATTTTAAAAAACTAACAGGTGTCAGCCTGTCCCGCTTATAAGATCATACGcc                                           >  2:1596026/1‑150 (MQ=255)
                                                                                                              gCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTCGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGtt                                        >  2:1705395/1‑150 (MQ=255)
                                                                                                              gCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGtt                                        >  2:2232419/1‑150 (MQ=255)
                                                                                                              gCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAGCTTCTCTGCCGCTTTTTTTTTCATCAATCAGCCCCCTAATCCTTGGTAGAGTATCACTTTTAAAAAACTAACAGTTGTCAGCCGGTCCCGCGGCTCAGATCATACGCCggg                                        >  2:490779/1‑148 (MQ=255)
                                                                                                                  gAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATAc                                    <  1:2027010/150‑1 (MQ=255)
                                                                                                                    gCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACgt                                  <  1:845632/150‑1 (MQ=255)
                                                                                                                       tCGCATCCAACAATGACCAGCGGTGGAGATCTTCTCAGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACgttgt                               <  2:1951562/150‑1 (MQ=255)
                                                                                                                       tCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCAAAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACgttgt                               >  1:637128/1‑150 (MQ=255)
                                                                                                                       tCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCAGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACgttgt                               <  2:1105391/150‑1 (MQ=255)
                                                                                                                        cGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACgttgtt                              >  2:1429973/1‑150 (MQ=255)
                                                                                                                        cGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACgttgtt                              >  2:1935266/1‑150 (MQ=255)
                                                                                                                         gCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGttt                             >  2:1787083/1‑150 (MQ=255)
                                                                                                                             ccAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGc                         <  1:2667787/150‑1 (MQ=255)
                                                                                                                              caacaaTGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCt                        >  2:123968/1‑150 (MQ=255)
                                                                                                                               aacaaTGACAAGCGTTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCATCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCtt                       <  1:2656993/150‑1 (MQ=255)
                                                                                                                               aacaaTGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCtt                       >  1:1880030/1‑150 (MQ=255)
                                                                                                                               aacaaTGACAAGCGGTGGAGATCTTCTCTGCCGCATTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCtt                       <  1:1870340/150‑1 (MQ=255)
                                                                                                                                  aaTGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGa                    <  2:863201/150‑1 (MQ=255)
                                                                                                                                  aaTGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGa                    <  2:863280/150‑1 (MQ=255)
                                                                                                                                  aaTGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGa                    <  2:863285/150‑1 (MQ=255)
                                                                                                                                        aaGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGGTATACGGTGTTTACGCTTTGAGGAATc              >  2:2653194/1‑150 (MQ=255)
                                                                                                                                        aaGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATAAATAAACCCCAAAAAACATTTTAGATTATAAATTTTAAAAAAAAAACAAGTGTCAGCCAGTACCGCATATAAGATAATAAGCCCTTATAAGTTGTTTACGCGTTTAGGaaac              >  1:555544/1‑148 (MQ=255)
                                                                                                                                        aaGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTAATCAATAAAACCCAAAAAACATTTTAGATTATAAATTTTAAAAAAAAAAAAGTTTTCAGCCTGTCACGCGTATAAAATCAAAAGCCGGTATAAGTTGTTTAAGCTTTTTGGAAta              >  1:1965326/1‑149 (MQ=255)
                                                                                                                                                gagaTCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTATCAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGAtga      <  2:2539283/150‑1 (MQ=255)
                                                                                                                                                gagaTCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGAtga      <  1:1987290/150‑1 (MQ=255)
                                                                                                                                                  gaTCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCGTATAAGATCATACGCCGTTATACGTTGGTTACGCTTTGAGGAATCCACGAtgagt    >  2:1865761/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
CAATATCGACGATTTCAGTGGCTTGATCATTAAAGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTGA  >  NC_000913/705803‑706100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: