Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,479,016 G→C 7.8% intergenic (+127/+18) rraB → / ← yjgM protein inhibitor of RNase E/GNAT family putative N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,479,0160GC 7.8% 624.1 / 34.8 177intergenic (+127/+18)rraB/yjgMprotein inhibitor of RNase E/GNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (85/78);  new base C (8/6);  total (93/84)
Fisher's exact test for biased strand distribution p-value = 7.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 178 total aligned reads displayed.

CGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAGATA  >  NC_000913/4478868‑4479165
                                                                                                                                                    |                                                                                                                                                     
cGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGa                                                                                                                                                      >  2:345583/1‑150 (MQ=255)
 gATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGaa                                                                                                                                                     <  2:13459/150‑1 (MQ=255)
        gAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCgg                                                                                                                                              <  1:146530/150‑1 (MQ=255)
         aGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCATGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCTGATAAGGCATTCATGTCACATCCGTCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCggg                                                                                                                                             <  1:1425019/150‑1 (MQ=255)
         aGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGTGTTCAACTCGCggg                                                                                                                                             <  2:194088/150‑1 (MQ=255)
         aGTTCGCCACTAATCAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGTGTTCAACTCGCggg                                                                                                                                             <  2:1448012/150‑1 (MQ=255)
              gCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCAttt                                                                                                                                        >  1:559662/1‑150 (MQ=255)
              gCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCAttt                                                                                                                                        >  2:2764154/1‑150 (MQ=255)
               ccACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCAtttt                                                                                                                                       <  2:573045/150‑1 (MQ=255)
               ccACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCAtttt                                                                                                                                       >  1:364210/1‑150 (MQ=255)
                cACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCAttttt                                                                                                                                      >  1:793086/1‑150 (MQ=255)
                 aCTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGATTTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCAtttttt                                                                                                                                     <  1:2125675/150‑1 (MQ=255)
                 aCTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCAtttttt                                                                                                                                     >  1:1584626/1‑150 (MQ=255)
                     aTTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAAGGCCCGCGAGTTGAACACGCGGGCATTTTTTTaga                                                                                                                                 <  1:1291051/150‑1 (MQ=255)
                      ttAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTagag                                                                                                                                >  1:728218/1‑150 (MQ=255)
                       tAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGt                                                                                                                               >  1:1540647/1‑150 (MQ=255)
                        aaGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGtt                                                                                                                              <  1:610772/150‑1 (MQ=255)
                            gTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTcgcg                                                                                                                          >  1:385930/1‑150 (MQ=255)
                            gTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTcgcg                                                                                                                          >  1:828310/1‑150 (MQ=255)
                                cgcATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAAc                                                                                                                      <  1:947559/150‑1 (MQ=255)
                                cgcATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGAGAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAAc                                                                                                                      <  1:1883558/150‑1 (MQ=255)
                                  cATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACAt                                                                                                                    >  1:2370474/1‑150 (MQ=255)
                                     cAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCg                                                                                                                 <  1:2113515/150‑1 (MQ=255)
                                        gCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCAc                                                                                                              >  1:64401/1‑150 (MQ=255)
                                        gCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCAc                                                                                                              >  1:175312/1‑150 (MQ=255)
                                          ccAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACtt                                                                                                            >  2:2762680/1‑150 (MQ=255)
                                            aGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTca                                                                                                          <  1:665616/150‑1 (MQ=255)
                                            aGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTca                                                                                                          <  1:918880/150‑1 (MQ=255)
                                            aGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTca                                                                                                          <  1:2123064/150‑1 (MQ=255)
                                                    ccTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGAc                                                                                                  <  1:2307885/150‑1 (MQ=255)
                                                    ccTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGAc                                                                                                  <  2:641856/150‑1 (MQ=255)
                                                     cTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTATAGTTCGCGCAACATCCGCACTTCACAATCGACa                                                                                                 >  2:383814/1‑150 (MQ=255)
                                                     cTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACa                                                                                                 >  2:1187384/1‑150 (MQ=255)
                                                     cTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACa                                                                                                 >  1:2158402/1‑150 (MQ=255)
                                                     cTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACa                                                                                                 >  2:2420691/1‑150 (MQ=255)
                                                        cAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATgg                                                                                              <  2:2192922/150‑1 (MQ=255)
                                                              ggTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTg                                                                                        >  1:654905/1‑150 (MQ=255)
                                                              ggTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTGTAGAGTTCGCGCAACATCCGCCCTTCACAATCGACATGGCCCGTg                                                                                        >  1:654934/1‑150 (MQ=255)
                                                               gTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTGTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGc                                                                                       >  2:1174561/1‑150 (MQ=255)
                                                                 gAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAg                                                                                     >  2:2207668/1‑150 (MQ=255)
                                                                  aaTTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGc                                                                                    <  2:559662/150‑1 (MQ=255)
                                                                     ttGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCaa                                                                                 <  1:2818600/150‑1 (MQ=255)
                                                                       gTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAgc                                                                               <  2:1963414/150‑1 (MQ=255)
                                                                          gCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCa                                                                            >  1:1203728/1‑150 (MQ=255)
                                                                          gCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCa                                                                            <  1:2055586/150‑1 (MQ=255)
                                                                           cccGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCAt                                                                           >  1:2266473/1‑150 (MQ=255)
                                                                           cccGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCAt                                                                           >  2:157579/1‑150 (MQ=255)
                                                                            ccGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATa                                                                          >  1:340291/1‑150 (MQ=255)
                                                                             cGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAg                                                                         >  1:2464245/1‑150 (MQ=255)
                                                                               gATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCTAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTc                                                                       <  2:760352/150‑1 (MQ=255)
                                                                                 tAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGa                                                                     <  1:415779/150‑1 (MQ=255)
                                                                                 tAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGa                                                                     <  1:2558744/150‑1 (MQ=255)
                                                                                     gCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATg                                                                 >  1:411213/1‑150 (MQ=255)
                                                                                     gCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGc                                                                >  2:161169/1‑150 (MQ=255)
                                                                                       aTTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCt                                                               >  1:1081719/1‑150 (MQ=255)
                                                                                       aTTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCt                                                               >  2:1326785/1‑150 (MQ=255)
                                                                                         tCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCa                                                             <  1:2849713/150‑1 (MQ=255)
                                                                                           aTGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCaaa                                                           >  1:1371804/1‑150 (MQ=255)
                                                                                            tGTCACATCCGGCATAAACAATACGTACGTTGTTAATGTTCGAATGCCCGCGTGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAg                                                          <  2:1604091/150‑1 (MQ=255)
                                                                                              tCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGTGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGcc                                                        <  1:2083845/150‑1 (MQ=255)
                                                                                              tCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGcc                                                        >  2:622987/1‑150 (MQ=255)
                                                                                              tCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGcc                                                        >  1:1311346/1‑150 (MQ=255)
                                                                                              tCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGcc                                                        >  1:2585614/1‑150 (MQ=255)
                                                                                                    ccGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCCAGCGCATAGTCGATATGCTCAAAGCCCAAATg                                                  >  1:204437/1‑150 (MQ=255)
                                                                                                     cGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGc                                                 >  1:1910053/1‑150 (MQ=255)
                                                                                                     cGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCAGAGTCGATATGCTCAACGCCCAACTGc                                                 >  2:1883827/1‑150 (MQ=255)
                                                                                                      ggCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCt                                                <  1:2354524/150‑1 (MQ=255)
                                                                                                           aaaCAAATCGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATaa                                           <  1:1707057/150‑1 (MQ=255)
                                                                                                            aaCAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATaaa                                          <  2:723326/150‑1 (MQ=255)
                                                                                                            aaCAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATaaa                                          <  2:279691/150‑1 (MQ=255)
                                                                                                             aCAAAACGTACGTTGTTAATGTTCGTATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAg                                         <  1:1679597/150‑1 (MQ=255)
                                                                                                              cAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAgc                                        <  2:1311346/150‑1 (MQ=255)
                                                                                                              cAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAgc                                        <  2:2074301/150‑1 (MQ=255)
                                                                                                                   cgtacgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAAt                                   <  2:2545944/150‑1 (MQ=255)
                                                                                                                   cgtacgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAAt                                   >  2:1471500/1‑150 (MQ=255)
                                                                                                                       cgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCt                               <  1:2549690/150‑1 (MQ=255)
                                                                                                                       cgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCt                               >  1:2483133/1‑150 (MQ=255)
                                                                                                                        gttgttAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCtt                              <  1:2764154/150‑1 (MQ=255)
                                                                                                                               aTGTTCGACTGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTaa                       <  2:1524119/150‑1 (MQ=255)
                                                                                                                               aTGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTaa                       <  2:2290930/150‑1 (MQ=255)
                                                                                                                               aTGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTaa                       <  1:2627333/150‑1 (MQ=255)
                                                                                                                                ttttCGACTGCCCGCGAGTTGAACTCGCGGGCATTTTTTTCGAGTTCGCGAAACATCCGCACTTCACAATCGACTTGGCCCGTGCAGCCAAGCGAATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTaaa                      <  2:1010166/148‑1 (MQ=255)
                                                                                                                                tGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTaaa                      <  2:175312/150‑1 (MQ=255)
                                                                                                                                  aaaaaaTGCCCGCGTGGTCAACTCGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTGCCTTTaaaaa                    >  2:2835012/5‑150 (MQ=255)
                                                                                                                                    cGAATGCCCGCGAGTTGAACACGCGGGCAATTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAg                  <  1:385146/150‑1 (MQ=255)
                                                                                                                                     gAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTc                                               <  1:1632850/120‑1 (MQ=255)
                                                                                                                                      aaTGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGATTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCATCCAAGAGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCg                <  2:1238975/150‑1 (MQ=255)
                                                                                                                                      aaTGCCCGCGAGTTGAACACGCGGGCATTTTGTTAGAGTTCGCGCGACATCCGCACTTCACAATCGACCTGGCCCGTGCAGCCCAGCGCAGCGGCGATATGCTCAAAGCCCAAATGCTCTTACAGCGCAATGGCTTCCTGTacaaccgcg                >  2:2336955/1‑145 (MQ=255)
                                                                                                                                          cccGCGTGTTCAACTCGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCg            >  2:2426564/1‑150 (MQ=255)
                                                                                                                                           ccGCGTGTTCAACTCGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGt           >  2:950780/1‑150 (MQ=255)
                                                                                                                                           ccGCGTGTTCAACTCGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGt           >  1:2013829/1‑150 (MQ=255)
                                                                                                                                           ccGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGt           >  1:621731/1‑150 (MQ=255)
                                                                                                                                             gcgTGTTCAACTCGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCACAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGttt         >  2:1869805/1‑150 (MQ=255)
                                                                                                                                             gcgAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGttt         >  2:1101981/1‑150 (MQ=255)
                                                                                                                                             gcgAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGtgt         >  2:453490/1‑148 (MQ=255)
                                                                                                                                               gAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTcc       >  2:114268/1‑150 (MQ=255)
                                                                                                                                                 gTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAg     >  1:1948434/1‑150 (MQ=255)
                                                                                                                                                  ttGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAGa    <  1:1901545/150‑1 (MQ=255)
                                                                                                                                                  ttGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACAGCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAGa    <  2:2485223/150‑1 (MQ=255)
                                                                                                                                                    gAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAGATa  <  2:2727869/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAGATA  >  NC_000913/4478868‑4479165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: