Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,549,932 T→A 6.2% intergenic (+222/+21) fimH → / ← gntP minor component of type 1 fimbriae/fructuronate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,549,9320TA 6.2% 724.2 / 19.5 232intergenic (+222/+21)fimH/gntPminor component of type 1 fimbriae/fructuronate transporter
Reads supporting (aligned to +/- strand):  ref base T (84/126);  new base A (15/2);  total (102/130)
Fisher's exact test for biased strand distribution p-value = 1.47e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

TAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGA  >  NC_000913/4549784‑4550081
                                                                                                                                                    |                                                                                                                                                     
ttggCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTGTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCAGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAtt                                                                                                                                                      >  2:8218/3‑150 (MQ=255)
 aGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTg                                                                                                                                                     <  2:2038044/150‑1 (MQ=255)
   gCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGc                                                                                                                                                   >  2:2431709/1‑150 (MQ=255)
   gCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCa                                                                                                                                                  >  1:297606/1‑150 (MQ=255)
    ccAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCCCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACAGTTTTAGACGTTGTCATCAGTCTGAGCCTGCCCTTGGcc                                                                                                                                                  >  1:2789452/1‑149 (MQ=255)
    ccAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCa                                                                                                                                                  <  2:453282/150‑1 (MQ=255)
    ccAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCa                                                                                                                                                  <  1:1469909/150‑1 (MQ=255)
     cAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAg                                                                                                                                                 <  1:633458/150‑1 (MQ=255)
     cAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAg                                                                                                                                                 <  1:1791673/150‑1 (MQ=255)
      aGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAgg                                                                                                                                                <  2:82420/150‑1 (MQ=255)
        aTAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGct                                                                                                                                              >  2:2410754/1‑150 (MQ=255)
          aaGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGctct                                                                                                                                            >  2:2781508/1‑150 (MQ=255)
           aGACGCGTCAGCGTCGCATCTGGCATAACCAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTg                                                                                                                                           <  1:451249/150‑1 (MQ=255)
           aGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTg                                                                                                                                           <  1:277428/150‑1 (MQ=255)
            gACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTgg                                                                                                                                          >  2:2347289/1‑150 (MQ=255)
            gACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTgg                                                                                                                                          <  2:1018628/150‑1 (MQ=255)
            gACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAATGGCAGGctcaga                                                                                                                                          <  2:366790/150‑4 (MQ=255)
             aCGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCACACCCAAACCCAATCCCCCGAGGGGCGAGGGGACTGTCCGGGCACATTTTTATACTTTGTCATAAGTCTGAGCCAGCCATTGGCAGGCTCTGgt                                                                                                                                         >  1:788142/1‑150 (MQ=255)
               gcgTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCACTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATATTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCAGgtgt                                                                                                                                       >  2:2354892/1‑150 (MQ=255)
                cgTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTACCCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTc                                                                                                                                      <  2:514416/150‑1 (MQ=255)
                cgTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTc                                                                                                                                      <  1:808240/150‑1 (MQ=255)
                cgTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTc                                                                                                                                      <  2:209516/150‑1 (MQ=255)
                    aGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCCCCCAAACCCACTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACAATTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCttt                                                                                                                                  >  1:2262080/1‑150 (MQ=255)
                       gTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTAc                                                                                                                               <  1:2136736/150‑1 (MQ=255)
                       gTCGCATCTGGCATAAACAAAGCGCACTATGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAGTGGCAGGCTCTGGTGTCCTTTTAc                                                                                                                               <  1:66727/150‑1 (MQ=255)
                        tCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTTTCATCAGTCTGAGCCTGCCATTGGCAGGCTCGGGTGTCCTGTTACg                                                                                                                              >  1:1655807/1‑150 (MQ=255)
                           cATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACGTTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTGTTACGCTa                                                                                                                           >  2:1462661/1‑150 (MQ=255)
                            aTCGGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTAc                                                                                                                          <  1:2501870/150‑1 (MQ=255)
                             tCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTAcc                                                                                                                         >  1:2068530/1‑150 (MQ=255)
                              cTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCa                                                                                                                        <  2:1911709/150‑1 (MQ=255)
                              cTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCGAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCa                                                                                                                        <  2:2230424/150‑1 (MQ=255)
                               tGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCAt                                                                                                                       <  1:215348/150‑1 (MQ=255)
                               tGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACGGGCCGGGCACCTGTTTAGTCGTTGTCATCAGTCTGAGCCTGCCATGGGCAGGCTCTGGTGTCCTGTTACGCTACCAt                                                                                                                       >  1:2527794/1‑150 (MQ=38)
                                  cATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCACACCCAAACCCACACCCCGGAGGGGCGAGGGGACTGTCCGGGCCCCTTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAGTGGCAGGCTCGGGTGTCCGTTTACGCTACCATGcg                                                                                                                    >  1:1727968/1‑149 (MQ=255)
                                   aTAAACAAAGCGCACTTTGCTGGTCTGTTCCCCACACCCAAAACCAATCCCCGGAGGGGCGAGGGGACTGTCCGGGCACAATTTTAGAATTTGTCATCAGTCTGAGCCGGCCCTTGGCAGGCTCTGGTGTGCTTTTAAGCTACCCTGCta                                                                                                                   >  1:1447287/1‑150 (MQ=255)
                                      aaCAAAGCGCACTTTGCTGGTCTGTTGCCCTCACCCTAACCCACTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTTTCATCAGTCTGAGCCTGCCATTGGCAGGCGCTGGTGTCCTTTTACGCTACCATGCtaata                                                                                                                >  1:1827993/1‑150 (MQ=255)
                                       aCAAAGCGCACTTTGCTGGTCTGTTCCCCACACCCAAACCCACACCCCCGAGGGGCGAGGGGCCGGTCCGGGCACAGTTTTATACGTTTTGATAAGTCTGAGCCAGCCATTTGCAGGGGCTGGTGTCCTTTTAAGCAACCAAGCtaaaaa                                                                                                               >  1:1310265/1‑150 (MQ=255)
                                        cAAAGCGCACTTTGCTGGTCTGTTCCCCGCACCCAAACCCACTCCCCCGAGGGGCGCGGGGACTGTCCGGGCACAATTTTAGACTTTGTGATCAGACTGAGCCAGCCCTTGGCAGGCTCAGGTGTCCTTTTAAGCAACCAAGCTaaaaat                                                                                                              >  1:2225681/1‑150 (MQ=255)
                                         aaaGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATc                                                                                                             <  2:2331264/150‑1 (MQ=255)
                                          aaGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCa                                                                                                            <  2:672952/150‑1 (MQ=255)
                                           aGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAg                                                                                                           <  1:33335/150‑1 (MQ=255)
                                             cgcACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGca                                                                                                         <  1:1524361/150‑1 (MQ=255)
                                                    tGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACaataat                                                                                                  <  2:2616650/150‑1 (MQ=255)
                                                    tGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACaataat                                                                                                  <  2:782895/150‑1 (MQ=255)
                                                    tGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACaataat                                                                                                  <  1:216923/150‑1 (MQ=255)
                                                     gCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCt                                                                                                                    >  2:59033/1‑131 (MQ=255)
                                                     gCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATc                                                                                                 <  1:2353259/150‑1 (MQ=255)
                                                     gCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATc                                                                                                 <  1:2410754/150‑1 (MQ=255)
                                                     gCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATc                                                                                                 <  1:2165861/150‑1 (MQ=255)
                                                       tGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAg                                                                                               <  1:1721165/150‑1 (MQ=255)
                                                       tGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAg                                                                                               <  2:1582245/150‑1 (MQ=255)
                                                        ggTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCAGCCGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTCATAATCAGCACAATAATCAGc                                                                                              >  2:2871304/1‑150 (MQ=255)
                                                        ggTCTGTTCCCCTCACCCGAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCTGGCACATTTTTAGACTTTGTCATCAGTCTGAGACTTCCATTTGCAGGCTCTGGTGTCCTTTTACGCTACCATGATAATAATCAGCACAATAATCAGc                                                                                              <  2:1253183/150‑1 (MQ=255)
                                                         gTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGcc                                                                                             <  2:2456220/150‑1 (MQ=255)
                                                         gTCTGTTCCCCTCACCCAAACCCTCACCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCAGGTGTACTTTTACGCTACCAAGCTAATAATCAGCACAATAATCAGcc                                                                                             >  2:90522/1‑150 (MQ=39)
                                                                ccccTCACCCTAACCCTCGCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCGACCATGCTAATAATCAGCACAATAATCAGCCCAACCAc                                                                                      <  1:1004407/150‑1 (MQ=255)
                                                                  ccTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATACTCAGCCCAACCACgg                                                                                    >  2:1533132/1‑150 (MQ=255)
                                                                  ccTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACgg                                                                                    >  2:2321798/1‑150 (MQ=255)
                                                                   cTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGa                                                                                   <  1:2326116/150‑1 (MQ=255)
                                                                   cTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGa                                                                                   <  1:583772/150‑1 (MQ=255)
                                                                    tCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAg                                                                                  <  1:1236442/150‑1 (MQ=255)
                                                                        ccTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGa                                                                              <  2:873635/150‑1 (MQ=255)
                                                                        ccTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGa                                                                              >  1:1582266/1‑150 (MQ=255)
                                                                         cTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGAc                                                                             <  2:1310265/150‑1 (MQ=255)
                                                                         cTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGAc                                                                             <  1:1959435/150‑1 (MQ=255)
                                                                              ccTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCt                                                                        >  1:772279/1‑150 (MQ=255)
                                                                                tctcCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTcc                                                                      >  1:912726/1‑150 (MQ=255)
                                                                                 ctcCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCca                                                                     <  1:2431980/150‑1 (MQ=255)
                                                                                  tcCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCcag                                                                    >  1:2611774/1‑150 (MQ=255)
                                                                                     ccGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCcagcag                                                                 >  2:1382016/1‑150 (MQ=255)
                                                                                            ggCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAg                                                          <  1:2574428/150‑1 (MQ=255)
                                                                                                aGGGGACTGTCCGTGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGtttt                                                      <  2:2567236/150‑1 (MQ=255)
                                                                                                  gggACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTc                                                    <  1:2481566/150‑1 (MQ=255)
                                                                                                    gACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCaa                                                  >  2:118134/1‑150 (MQ=255)
                                                                                                           cGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCt                                           >  2:2256416/1‑150 (MQ=255)
                                                                                                                 caTTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACt                                     <  2:731859/150‑1 (MQ=255)
                                                                                                                 caTTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACt                                     <  1:573459/150‑1 (MQ=255)
                                                                                                                 caTTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACt                                     <  2:1666949/150‑1 (MQ=255)
                                                                                                                 caTTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACt                                     <  1:1028192/150‑1 (MQ=255)
                                                                                                                   tttttAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGa                                   <  2:2132577/150‑1 (MQ=255)
                                                                                                                     tttAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACa                                 <  1:2109139/150‑1 (MQ=255)
                                                                                                                     tttAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTGTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACa                                 <  1:793176/150‑1 (MQ=255)
                                                                                                                          aCTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCa                            <  1:1541190/150‑1 (MQ=255)
                                                                                                                            tttGTCATCAGTCTGAGCCTGCCCTTGGCAGGCTCGGGTGTCCGTTTACGCTACCATGCTACTCATCAGCACAATAATCAGCCCAACCACGGAGGTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACGGACAGGTCaag                          >  2:316758/1‑149 (MQ=255)
                                                                                                                            tttGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCaaa                          <  2:2099790/150‑1 (MQ=255)
                                                                                                                                   tcaGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTAAGCTACCATGCTAATAATCAGCACAATAATAAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCt                   >  1:1069615/1‑150 (MQ=255)
                                                                                                                                    caGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCtt                  <  2:699485/150‑1 (MQ=255)
                                                                                                                                    caGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCtt                  <  2:933706/150‑1 (MQ=255)
                                                                                                                                     aGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCttt                 <  1:1011966/150‑1 (MQ=255)
                                                                                                                                      accagAGCCTGCCAATGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACgt                                               >  2:1990164/5‑119 (MQ=255)
                                                                                                                                      accagAGCCTGCCAATGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACgt                                               <  1:1990164/115‑1 (MQ=255)
                                                                                                                                       tCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGa               <  1:426200/150‑1 (MQ=255)
                                                                                                                                       tCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGa               >  1:2058417/1‑150 (MQ=255)
                                                                                                                                       tCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCCAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGa               >  1:1934305/1‑150 (MQ=255)
                                                                                                                                         agAGCCTGCCAATGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGaac             >  1:320868/2‑150 (MQ=255)
                                                                                                                                         agAGCCTGCCAATGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGaac             >  1:2186377/2‑150 (MQ=255)
                                                                                                                                           aGCCTGCCAATGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGaacaa           >  2:2751233/1‑150 (MQ=255)
                                                                                                                                            gCCTGCAATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCATACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGaacaac          <  1:2602842/150‑1 (MQ=255)
                                                                                                                                               tGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAg       >  2:2520292/1‑150 (MQ=255)
                                                                                                                                                 ccATTGGCAGGCTCTGGTGTCCTTTTACGCTACCAGGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGaa     <  2:1757553/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGA  >  NC_000913/4549784‑4550081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: