BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	781,238	T→A	7.2%	intergenic (+16/‑131)	lysY → / → lysZ	tRNA‑Lys/tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	781,238	0	T	A	7.2%	319.7 / 6.4	111	intergenic (+16/‑131)	lysY/lysZ	tRNA‑Lys/tRNA‑Lys
Reads supporting (aligned to +/- strand): ref base T (56/41); new base A (7/3); total (67/44)
Fisher's exact test for biased strand distribution p-value = 5.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

GAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGG  >  NC_000913/781089‑781387

gagCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACt                                                                                                                                                       >  1:2264866/1‑150 (MQ=38)

    aCCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGGAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTATAAAAACAAAATCAGa                                                                                                                                                   >  1:1452862/1‑150 (MQ=11)

      cTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATg                                                                                                                                                 <  1:2610673/150‑1 (MQ=255)

      cTCACTTACATGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTAATTTTGTAGAGCAGTTGACTTTTAATCATTTGGTCGCAGGTTCGAATCCTGCACGACCCTCCAGTTTTAACATCAAACTCAGATg                                                                                                                                                 <  1:216614/150‑1 (MQ=14)

       tCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCCCGACCCACCAGTTTTAACATCAAACACAGATGt                                                                                                                                                >  1:344232/1‑150 (MQ=255)

       tCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAAATAAGATGt                                                                                                                                                >  1:344219/1‑150 (MQ=255)

          cTTACACGGAGGGGGTCGGGGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTaa                                                                                                                                             <  1:2789275/150‑1 (MQ=255)

           ttACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAg                                                                                                                                            >  2:2757340/1‑150 (MQ=255)

            tACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGc                                                                                                                                           <  2:1051807/150‑1 (MQ=255)

            tACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGc                                                                                                                                           <  1:1359120/150‑1 (MQ=255)

             aCAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCg                                                                                                                                          <  2:1647337/150‑1 (MQ=255)

              cAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCTTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGt                                                                                                                                         <  2:343417/150‑1 (MQ=255)

                     ggggTCGGCGGTTTGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGAt                                                                                                                                  <  2:2809688/150‑1 (MQ=255)

                         tCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCATACTCAGTTGTTAAGCGTGAAGGTTAACg                                                                                                                              >  2:2883320/1‑150 (MQ=255)

                           ggcggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGtt                                                                                                                            >  2:712983/1‑150 (MQ=255)

                             cggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGt                                                                                                                                         <  1:503781/135‑1 (MQ=255)

                             cggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGt                                                                                                                                         >  2:503781/1‑135 (MQ=255)

                              ggctCGATCCCGTCAAAACCCACCACTCGGTTCGTTATCTCAGTTGGTAGAGCAGTTTACTTTGTTTCAATTTGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgcg                                                                                                                         <  1:554687/147‑1 (MQ=14)

                              ggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgcg                                                                                                                         <  2:1552758/150‑1 (MQ=255)

                              ggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAAAATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgcg                                                                                                                         >  1:1113202/1‑150 (MQ=255)

                              ggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAACCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgcg                                                                                                                         <  1:1687978/150‑1 (MQ=255)

                              ggTTAGTTCCCCTCCACACCCACCCCTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgcg                                                                                                                         <  1:2443485/150‑1 (MQ=14)

                                 tCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCa                                                                                                                      <  2:1471165/150‑1 (MQ=255)

                                 tCGATCCCGACAACACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGCTCGCAGGATCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCa                                                                                                                      <  1:894108/150‑1 (MQ=255)

                                    aTCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTTAGTTTTAATCAATTGGTCGCAGGTTAGAATACTGCACGACCCACCAGTTTTTAAATCAAAATAAGATTTTAATCGTGAATGATAAAGTTGCTCCAGCa                                                                                                                   >  1:1321274/1‑150 (MQ=14)

                                        cGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGGTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCCCCAGTTTTAACAGAAAAAACAGAGGTTAAGCGTGAAGGATAACGTTGGGCCACCCACgg                                                                                                               >  1:2047218/1‑150 (MQ=14)

                                         gTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTAGCAGGTTCGAATCCTGCACGACCCACCAGTATTAACATCAAACGAAGAGGTTAATCGGGAAGGATAACTTTGCGCCAGCAACGGc                                                                                                              >  1:385917/1‑150 (MQ=14)

                                          tcatcaCCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTAAACGTCGATAGAGGATGTGAAGGGGGAGGGATACGGTTGCGGCGGCAACGGcc                                                                                                             >  2:1321127/1‑150 (MQ=11)

                                           catcaCCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAAAATCAAAATCAGATGTTAACCGTGAAGGATAACGTTGCGCCAGAAACGGccc                                                                                                            >  1:63230/1‑150 (MQ=255)

                                                            gTCGTTAGCTCAGTTGGTATATCAGTTGACATTAAATCAATAGGTCGAAGCTTCGAATCCAGCACTACCCACCACAATTAACAACAAAATCAGATGTTAAGAGTGAAGGATAACGTTGCGCCATCAACGCCCCGTAGTGCGagcgaagcg                                                                                           >  2:2559927/1‑150 (MQ=14)

                                                              gttgaGCTCAGTTGGTAGAGCAGTTGTCTTTTAATCAATGTGTCGCAGGTTCTAATCCTTCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAg                                                                                         <  2:2459590/146‑1 (MQ=14)

                                                              ctttAGCTCAGTTGGTAGATCTTTTGTCTCTTAATCAATTGGTCGCTCGACGTCCAACTTCAGTTCCCACCAGTTTTAACATCTAACTCAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAg                                                                                         <  2:1163189/148‑1 (MQ=2)

                                                               gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAATCGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTATAACATCAAACGCAGAAGATAACCATAAAGGAAAACGTTGCGCCACCAACGGCCCGTAGGGCGAGCGAAGCGAGt                                                                                        >  2:2022691/1‑150 (MQ=14)

                                                                ttAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTc                                                                                       >  1:2750682/1‑150 (MQ=255)

                                                                     tCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCt                                                                                  >  2:2096758/1‑150 (MQ=255)

                                                                     tCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAACCGTGAAGGATAACGTTGCGCAAGCAACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCt                                                                                  >  2:1922813/1‑150 (MQ=255)

                                                                        gTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACACAGATGATAAGCGTGAAGGATAACGATGCGCCAGCAACGGCACGAAGGGCGAGCGAAGCGAGTCAACATGCa                                                                               >  2:2543965/1‑150 (MQ=11)

                                                                        gTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCACTTTTAACATCAAACACAGAAGTTAAGCGTGAAGGAAAACGTTCCTACACAAACGCCCCGAAGGGCGAGCGAAGCGAGTCaaacagca                                                                               >  2:383696/1‑143 (MQ=11)

                                                                         ttGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACACAGATTTTAAGAGTGAAGGATAACGATGCTCAAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCAc                                                                              >  2:299504/1‑150 (MQ=11)

                                                                           ggTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCAGCACGACCCACCAGTTTTAACATCAAACTCAGATGTAAAGCGTGAAGGATAACGATGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGa                                                                            >  2:1640483/1‑150 (MQ=255)

                                                                             tAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGc                                                                                                              <  2:2791274/114‑1 (MQ=255)

                                                                             tAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGc                                                                                                              >  1:2791274/1‑114 (MQ=255)

                                                                             tAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAACCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGAcc                                                                          >  1:1185690/1‑150 (MQ=255)

                                                                                  cAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacca                                                                     <  2:1783548/150‑1 (MQ=255)

                                                                                    gTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGGAGCGAGTCAACCTGCACGACCCACCAAt                                                                   >  1:346580/1‑150 (MQ=255)

                                                                                     ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATc                                                                  >  2:2488863/1‑150 (MQ=255)

                                                                                     ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATc                                                                  >  1:165092/1‑150 (MQ=255)

                                                                                     ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATc                                                                  >  1:1797711/1‑150 (MQ=255)

                                                                                       gACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCAGCACGACCCACCAATCtt                                                                >  1:1135601/1‑150 (MQ=255)

                                                                                        aCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTa                                                               >  1:2692550/1‑150 (MQ=255)

                                                                                        aCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTa                                                               <  2:2061493/150‑1 (MQ=255)

                                                                                          ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTa                                                                                                         >  1:2150861/1‑106 (MQ=255)

                                                                                          ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaaa                                                             <  2:723861/150‑1 (MQ=255)

                                                                                            ttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAACTCAGATGTTAAGCGTGAAGGATaa                                                                                                                                >  2:1349184/1‑81 (MQ=255)

                                                                                            ttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATaa                                                                                                                                <  1:1349184/81‑1 (MQ=255)

                                                                                              aaTCAATTGGTCGCAGGTTCGAATCCTGCACGTCCCACCAGTTTTAACATCTATCTCTGATGTTAAGCGTTAAGGATATCGTTGCGCGAGGATCGTCCGGTATGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGAtt                                                         <  2:2757155/150‑1 (MQ=17)

                                                                                              aaTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCTCCAGTTTTAACATCAAACTCATATGTTAAGCGTGAAGGATATCGTTGCGCCAGGAACGGCCGGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGAtt                                                         <  2:1751920/150‑1 (MQ=17)

                                                                                                  aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACACTGAAAGATTGGcc                                                     >  1:498739/1‑150 (MQ=255)

                                                                                                  aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGcc                                                     >  2:2000122/1‑150 (MQ=255)

                                                                                                  aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCAGCACGACCCACCAATCTTAAAGAGTGGcc                                                     >  1:2263872/1‑150 (MQ=255)

                                                                                                  aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCAGCACGACCCACCAATCTGAAAGATTGGcc                                                     >  1:113306/1‑150 (MQ=255)

                                                                                                    ttGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCTAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGcccc                                                   <  2:2505059/150‑1 (MQ=255)

                                                                                                       gTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAg                                                >  1:321006/1‑150 (MQ=255)

                                                                                                       gTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAg                                                >  1:984004/1‑150 (MQ=255)

                                                                                                          gCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaaa                                                             >  1:1643282/1‑134 (MQ=255)

                                                                                                          gCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaaa                                                             <  2:1643282/134‑1 (MQ=255)

                                                                                                          gCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTaa                                             >  1:1845614/1‑150 (MQ=255)

                                                                                                            aGGTTCGAATCCTGCACGACCCACCAGTTTTATCATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGTCGCGAGTCATCCTGCACGACCCACCAAGCTTAAAGATTGGCCCCGGGTaaat                                           >  1:1754658/1‑149 (MQ=17)

                                                                                                            aGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACGCAGAAGTTAAGCGTGAAGGATAACGTGCCGCCGGCAACGGCCCCAGGGGCGGGCGAAGCGAGTAATCCGTCACGACCCCCCCATCTTAAAGATTGGAGCCGGGTaaaa                                           >  1:1754690/1‑150 (MQ=17)

                                                                                                              gTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAAt                                         <  1:2333026/150‑1 (MQ=255)

                                                                                                                  gAATCCTGCAATACCCACTTTTTATTCAATAAAAATCAGATGTTAAGCGATAAGGATAACGTTGCGACACCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCttt                                     <  1:117967/150‑1 (MQ=18)

                                                                                                                    aTCCTGCACGACCCACCAGTTTTAACATCTAACTCAGATTTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCGCTTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCa                                   <  2:116310/150‑1 (MQ=255)

                                                                                                                    aTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCa                                   <  1:712983/150‑1 (MQ=255)

                                                                                                                       cTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGt                                >  1:1995971/1‑150 (MQ=255)

                                                                                                                       cTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGt                                >  1:1995972/1‑150 (MQ=255)

                                                                                                                       cTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGt                                >  1:565839/1‑150 (MQ=255)

                                                                                                                       cTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGt                                >  2:1783654/1‑150 (MQ=255)

                                                                                                                        tGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAGCGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTa                               >  1:1356055/1‑150 (MQ=255)

                                                                                                                        tGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTa                               >  1:1983779/1‑150 (MQ=255)

                                                                                                                            cGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAacac                           >  1:1279117/1‑150 (MQ=255)

                                                                                                                                ccaccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGt                       >  1:2635426/1‑150 (MQ=255)

                                                                                                                                   ccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATg                    >  2:1776898/1‑150 (MQ=255)

                                                                                                                                   ccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATg                    >  1:789433/1‑150 (MQ=255)

                                                                                                                                    caGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATgg                   <  2:1233353/150‑1 (MQ=18)

                                                                                                                                    caGTTTTAACATCCAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATgg                   <  2:437513/150‑1 (MQ=255)

                                                                                                                                     aGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATggg                  >  1:530964/1‑150 (MQ=17)

                                                                                                                                      gTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGt                 <  2:2724722/150‑1 (MQ=255)

                                                                                                                                       ttttACCATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTc                <  1:2496544/150‑1 (MQ=255)

                                                                                                                                       ttttAACATCAAACACAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTc                <  1:2496559/150‑1 (MQ=255)

                                                                                                                                         ttAACATCACACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGt              <  1:2276592/150‑1 (MQ=255)

                                                                                                                                         ttAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGt              <  1:1079369/150‑1 (MQ=255)

                                                                                                                                         ttAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGt              <  2:1775616/150‑1 (MQ=255)

                                                                                                                                             cATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAAACATACAGGTAACACCCGTATGGGTCGTTAGc          >  1:2791426/1‑150 (MQ=255)

                                                                                                                                              aTCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCAACCTGCACGACCCACCAAACATAAAGATTGGCCCCGAGAAAAAAACGTAAAGGTAACACACGAAAGGGTCGTTAGca         >  1:1931375/1‑149 (MQ=18)

                                                                                                                                                ccaccTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGACGAGCGACGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCa       <  2:1488000/146‑1 (MQ=255)

                                                                                                                                                  aaCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGt     <  1:111491/150‑1 (MQ=255)

                                                                                                                                                     tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTgg  >  1:382577/1‑150 (MQ=255)

                                                                                                                                                     tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTgg  >  1:1297577/1‑150 (MQ=255)

                                                                                                                                                     tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTgg  <  1:2748633/150‑1 (MQ=255)

                                                                                                                                                     tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGGATGGGTCGTTAGCTCAGTTgg  >  1:2870851/1‑150 (MQ=255)

GAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGG  >  NC_000913/781089‑781387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`