BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	781,254	A→T	7.8%	intergenic (+32/‑115)	lysY → / → lysZ	tRNA‑Lys/tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	781,254	0	A	T	7.8%	327.1 / 9.8	109	intergenic (+32/‑115)	lysY/lysZ	tRNA‑Lys/tRNA‑Lys
Reads supporting (aligned to +/- strand): ref base A (62/37); new base T (4/6); total (66/43)
Fisher's exact test for biased strand distribution p-value = 1.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

GGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTT  >  NC_000913/781106‑781403

    ggggTCGGCGGTTTGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGAt                                                                                                                                                  <  2:2809688/150‑1 (MQ=255)

        tCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCATACTCAGTTGTTAAGCGTGAAGGTTAACg                                                                                                                                              >  2:2883320/1‑150 (MQ=255)

          ggcggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGtt                                                                                                                                            >  2:712983/1‑150 (MQ=255)

           gcggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTg                                                                                                                                           >  2:2745998/1‑150 (MQ=255)

             ggctCGATCCCGTCAAAACCCACCACTCGGTTCGTTATCTCAGTTGGTAGAGCAGTTTACTTTGTTTCAATTTGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgcg                                                                                                                                         <  1:554687/147‑1 (MQ=14)

             ggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAAAATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgcg                                                                                                                                         >  1:1113202/1‑150 (MQ=255)

             ggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAACCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgcg                                                                                                                                         <  1:1687978/150‑1 (MQ=255)

             ggTTAGTTCCCCTCCACACCCACCCCTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgcg                                                                                                                                         <  1:2443485/150‑1 (MQ=14)

             ggTCCGATCCCGTCAACACCCACCCCTCGGCTCGTTATCTCAGTTGGTTGAGCAGTTGTCTTTTCAGCTATTGGACGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgcg                                                                                                                                         <  1:2889234/150‑1 (MQ=14)

                tCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCa                                                                                                                                      <  2:1471165/150‑1 (MQ=255)

                tCGATCCCGACAACACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGCTCGCAGGATCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCa                                                                                                                                      <  1:894108/150‑1 (MQ=255)

                   aTCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTTAGTTTTAATCAATTGGTCGCAGGTTAGAATACTGCACGACCCACCAGTTTTTAAATCAAAATAAGATTTTAATCGTGAATGATAAAGTTGCTCCAGCa                                                                                                                                   >  1:1321274/1‑150 (MQ=14)

                       cGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGGTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCCCCAGTTTTAACAGAAAAAACAGAGGTTAAGCGTGAAGGATAACGTTGGGCCACCCACgg                                                                                                                               >  1:2047218/1‑150 (MQ=14)

                        gTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTAGCAGGTTCGAATCCTGCACGACCCACCAGTATTAACATCAAACGAAGAGGTTAATCGGGAAGGATAACTTTGCGCCAGCAACGGc                                                                                                                              >  1:385917/1‑150 (MQ=14)

                         tcatcaCCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTAAACGTCGATAGAGGATGTGAAGGGGGAGGGATACGGTTGCGGCGGCAACGGcc                                                                                                                             >  2:1321127/1‑150 (MQ=11)

                          catcaCCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAAAATCAAAATCAGATGTTAACCGTGAAGGATAACGTTGCGCCAGAAACGGccc                                                                                                                            >  1:63230/1‑150 (MQ=255)

                                           gTCGTTAGCTCAGTTGGTATATCAGTTGACATTAAATCAATAGGTCGAAGCTTCGAATCCAGCACTACCCACCACAATTAACAACAAAATCAGATGTTAAGAGTGAAGGATAACGTTGCGCCATCAACGCCCCGTAGTGCGagcgaagcg                                                                                                           >  2:2559927/1‑150 (MQ=14)

                                             gttgaGCTCAGTTGGTAGAGCAGTTGTCTTTTAATCAATGTGTCGCAGGTTCTAATCCTTCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAg                                                                                                         <  2:2459590/146‑1 (MQ=14)

                                             ctttAGCTCAGTTGGTAGATCTTTTGTCTCTTAATCAATTGGTCGCTCGACGTCCAACTTCAGTTCCCACCAGTTTTAACATCTAACTCAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAg                                                                                                         <  2:1163189/148‑1 (MQ=2)

                                              gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAATCGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTATAACATCAAACGCAGAAGATAACCATAAAGGAAAACGTTGCGCCACCAACGGCCCGTAGGGCGAGCGAAGCGAGt                                                                                                        >  2:2022691/1‑150 (MQ=14)

                                               ttAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTc                                                                                                       >  1:2750682/1‑150 (MQ=255)

                                                    tCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCt                                                                                                  >  2:2096758/1‑150 (MQ=255)

                                                       gTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACACAGATGATAAGCGTGAAGGATAACGATGCGCCAGCAACGGCACGAAGGGCGAGCGAAGCGAGTCAACATGCa                                                                                               >  2:2543965/1‑150 (MQ=11)

                                                       gTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCACTTTTAACATCAAACACAGAAGTTAAGCGTGAAGGAAAACGTTCCTACACAAACGCCCCGAAGGGCGAGCGAAGCGAGTCaaacagca                                                                                               >  2:383696/1‑143 (MQ=11)

                                                        ttGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACACAGATTTTAAGAGTGAAGGATAACGATGCTCAAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCAc                                                                                              >  2:299504/1‑150 (MQ=11)

                                                          ggTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCAGCACGACCCACCAGTTTTAACATCAAACTCAGATGTAAAGCGTGAAGGATAACGATGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGa                                                                                            >  2:1640483/1‑150 (MQ=255)

                                                            tAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGc                                                                                                                              >  1:2791274/1‑114 (MQ=255)

                                                            tAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGc                                                                                                                              <  2:2791274/114‑1 (MQ=255)

                                                            tAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAACCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGAcc                                                                                          >  1:1185690/1‑150 (MQ=255)

                                                                 cAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacca                                                                                     <  2:1783548/150‑1 (MQ=255)

                                                                    ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATc                                                                                  >  1:1797711/1‑150 (MQ=255)

                                                                    ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATc                                                                                  >  1:165092/1‑150 (MQ=255)

                                                                    ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATc                                                                                  >  2:2488863/1‑150 (MQ=255)

                                                                      gACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCAGCACGACCCACCAATCtt                                                                                >  1:1135601/1‑150 (MQ=255)

                                                                       aCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTa                                                                               >  1:2692550/1‑150 (MQ=255)

                                                                       aCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTa                                                                               <  2:2061493/150‑1 (MQ=255)

                                                                         ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTa                                                                                                                         <  2:2150861/106‑1 (MQ=255)

                                                                         ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaaa                                                                             <  2:723861/150‑1 (MQ=255)

                                                                          tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCATAAAg                                                                            >  1:2718059/1‑150 (MQ=255)

                                                                           ttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAACTCAGATGTTAAGCGTGAAGGATaa                                                                                                                                                >  2:1349184/1‑81 (MQ=255)

                                                                           ttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATaa                                                                                                                                                <  1:1349184/81‑1 (MQ=255)

                                                                             aaTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCTCCAGTTTTAACATCAAACTCATATGTTAAGCGTGAAGGATATCGTTGCGCCAGGAACGGCCGGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGAtt                                                                         <  2:1751920/150‑1 (MQ=17)

                                                                              aTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTg                                                                        >  1:2022421/1‑150 (MQ=255)

                                                                               tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTgg                                                                       >  2:1563704/1‑150 (MQ=255)

                                                                                 aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACACTGAAAGATTGGcc                                                                     >  1:498739/1‑150 (MQ=255)

                                                                                 aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGcc                                                                     >  2:2000122/1‑150 (MQ=255)

                                                                                 aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCAGCACGACCCACCAATCTTAAAGAGTGGcc                                                                     >  1:2263872/1‑150 (MQ=255)

                                                                                 aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCAGCACGACCCACCAATCTGAAAGATTGGcc                                                                     >  1:113306/1‑150 (MQ=255)

                                                                                      gTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAg                                                                >  1:321006/1‑150 (MQ=255)

                                                                                      gTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAg                                                                >  1:984004/1‑150 (MQ=255)

                                                                                       tCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGt                                                               >  1:1233353/1‑150 (MQ=11)

                                                                                         gCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaaa                                                                             >  1:1643282/1‑134 (MQ=255)

                                                                                         gCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaaa                                                                             <  2:1643282/134‑1 (MQ=255)

                                                                                         gCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTaa                                                             >  1:1845614/1‑150 (MQ=255)

                                                                                           aGGTTCGAATCCTGCACGACCCACCAGTTTTATCATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGTCGCGAGTCATCCTGCACGACCCACCAAGCTTAAAGATTGGCCCCGGGTaaat                                                           >  1:1754658/1‑149 (MQ=17)

                                                                                             gTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAAt                                                         <  1:2333026/150‑1 (MQ=255)

                                                                                                 gAATCCTGCAATACCCACTTTTTATTCAATAAAAATCAGATGTTAAGCGATAAGGATAACGTTGCGACACCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCttt                                                     <  1:117967/150‑1 (MQ=18)

                                                                                                   aTCCTGCACGACCCACCAGTTTTAACATCTAACTCAGATTTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCGCTTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCa                                                   <  2:116310/150‑1 (MQ=255)

                                                                                                   aTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCa                                                   <  1:712983/150‑1 (MQ=255)

                                                                                                      cTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGt                                                >  2:1783654/1‑150 (MQ=255)

                                                                                                      cTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGt                                                >  1:1995971/1‑150 (MQ=255)

                                                                                                      cTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGt                                                >  1:1995972/1‑150 (MQ=255)

                                                                                                      cTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGt                                                >  1:565839/1‑150 (MQ=255)

                                                                                                       tGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAGCGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTa                                               >  1:1356055/1‑150 (MQ=255)

                                                                                                       tGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTa                                               >  1:1983779/1‑150 (MQ=255)

                                                                                                           cGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAacac                                           >  1:1279117/1‑150 (MQ=255)

                                                                                                               ccaccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGt                                       >  1:2635426/1‑150 (MQ=255)

                                                                                                                  ccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATg                                    >  2:1776898/1‑150 (MQ=255)

                                                                                                                  ccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATg                                    >  2:1776935/1‑150 (MQ=255)

                                                                                                                   caGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATgg                                   <  2:1233353/150‑1 (MQ=18)

                                                                                                                   caGTTTTAACATCCAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATgg                                   <  2:437513/150‑1 (MQ=255)

                                                                                                                    aGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATggg                                  >  1:530964/1‑150 (MQ=17)

                                                                                                                     gTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGt                                 <  2:2724722/150‑1 (MQ=255)

                                                                                                                      ttttACCATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTc                                <  1:2496544/150‑1 (MQ=255)

                                                                                                                      ttttAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTc                                >  1:1792348/1‑150 (MQ=255)

                                                                                                                      ttttAACATCAAACACAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTc                                <  1:2496559/150‑1 (MQ=255)

                                                                                                                        ttAACATCACACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGt                              <  1:2276592/150‑1 (MQ=255)

                                                                                                                        ttAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGt                              <  2:1775616/150‑1 (MQ=255)

                                                                                                                        ttAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGt                              <  1:1079369/150‑1 (MQ=255)

                                                                                                                            cATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAAACATACAGGTAACACCCGTATGGGTCGTTAGc                          >  1:2791426/1‑150 (MQ=255)

                                                                                                                             aTCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCAACCTGCACGACCCACCAAACATAAAGATTGGCCCCGAGAAAAAAACGTAAAGGTAACACACGAAAGGGTCGTTAGca                         >  1:1931375/1‑149 (MQ=18)

                                                                                                                              tCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAAACTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGAATGGGTCGATAGCTc                        >  1:1377789/1‑150 (MQ=255)

                                                                                                                               cgaagacAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCa                       <  2:530964/144‑1 (MQ=255)

                                                                                                                               ccaccTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGACGAGCGACGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCa                       <  2:1488000/146‑1 (MQ=255)

                                                                                                                                gaagacAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAg                      >  2:2514885/6‑150 (MQ=255)

                                                                                                                                 aaCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGt                     <  1:111491/150‑1 (MQ=255)

                                                                                                                                  agacAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGtt                    <  1:2514885/147‑1 (MQ=255)

                                                                                                                                    tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTgg                  >  1:382577/1‑150 (MQ=255)

                                                                                                                                    tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTgg                  <  1:2748633/150‑1 (MQ=255)

                                                                                                                                    tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGGATGGGTCGTTAGCTCAGTTgg                  >  1:2870851/1‑150 (MQ=255)

                                                                                                                                     cAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGt                 <  1:263318/150‑1 (MQ=255)

                                                                                                                                     cAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGt                 <  2:2022421/150‑1 (MQ=255)

                                                                                                                                      aGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTa                >  1:643315/1‑150 (MQ=255)

                                                                                                                                        aTGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTaga              >  1:1561127/1‑150 (MQ=255)

                                                                                                                                           ttAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCa           >  2:2731777/1‑150 (MQ=255)

                                                                                                                                           ttAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCa           <  2:2019484/150‑1 (MQ=255)

                                                                                                                                                 gTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAc     <  2:2692550/150‑1 (MQ=255)

                                                                                                                                                  tGACGGATACCGTTGCGCCAGCACCGGCCCGCAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACt    <  2:2562111/150‑1 (MQ=255)

                                                                                                                                                  tGAAGGATAACGTAGCGGCAGCAACGGCGAGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACt    <  2:2562076/150‑1 (MQ=255)

                                                                                                                                                    aaGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACttt  <  1:1163540/150‑1 (MQ=255)

GGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTT  >  NC_000913/781106‑781403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`