BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	781,269	C→T	6.2%	intergenic (+47/‑100)	lysY → / → lysZ	tRNA‑Lys/tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	781,269	0	C	T	6.2%	348.7 / 8.2	118	intergenic (+47/‑100)	lysY/lysZ	tRNA‑Lys/tRNA‑Lys
Reads supporting (aligned to +/- strand): ref base C (62/43); new base T (4/3); total (70/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

TCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGC  >  NC_000913/781122‑781418

   aTCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTTAGTTTTAATCAATTGGTCGCAGGTTAGAATACTGCACGACCCACCAGTTTTTAAATCAAAATAAGATTTTAATCGTGAATGATAAAGTTGCTCCAGCa                                                                                                                                                  >  1:1321274/1‑150 (MQ=14)

       cGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGGTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCCCCAGTTTTAACAGAAAAAACAGAGGTTAAGCGTGAAGGATAACGTTGGGCCACCCACgg                                                                                                                                              >  1:2047218/1‑150 (MQ=14)

        gTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTAGCAGGTTCGAATCCTGCACGACCCACCAGTATTAACATCAAACGAAGAGGTTAATCGGGAAGGATAACTTTGCGCCAGCAACGGc                                                                                                                                             >  1:385917/1‑150 (MQ=14)

         tcatcaCCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTAAACGTCGATAGAGGATGTGAAGGGGGAGGGATACGGTTGCGGCGGCAACGGcc                                                                                                                                            >  2:1321127/1‑150 (MQ=11)

                           gTCGTTAGCTCAGTTGGTATATCAGTTGACATTAAATCAATAGGTCGAAGCTTCGAATCCAGCACTACCCACCACAATTAACAACAAAATCAGATGTTAAGAGTGAAGGATAACGTTGCGCCATCAACGCCCCGTAGTGCGagcgaagcg                                                                                                                          >  2:2559927/1‑150 (MQ=14)

                             gttgaGCTCAGTTGGTAGAGCAGTTGTCTTTTAATCAATGTGTCGCAGGTTCTAATCCTTCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAg                                                                                                                        <  2:2459590/146‑1 (MQ=14)

                             ctttAGCTCAGTTGGTAGATCTTTTGTCTCTTAATCAATTGGTCGCTCGACGTCCAACTTCAGTTCCCACCAGTTTTAACATCTAACTCAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAg                                                                                                                        <  2:1163189/148‑1 (MQ=2)

                              gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAATCGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTATAACATCAAACGCAGAAGATAACCATAAAGGAAAACGTTGCGCCACCAACGGCCCGTAGGGCGAGCGAAGCGAGt                                                                                                                       >  2:2022691/1‑150 (MQ=14)

                                    tCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCt                                                                                                                 >  2:2096758/1‑150 (MQ=255)

                                    tCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAACCGTGAAGGATAACGTTGCGCAAGCAACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCt                                                                                                                 >  2:1922813/1‑150 (MQ=255)

                                       gTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACACAGATGATAAGCGTGAAGGATAACGATGCGCCAGCAACGGCACGAAGGGCGAGCGAAGCGAGTCAACATGCa                                                                                                              >  2:2543965/1‑150 (MQ=11)

                                        ttGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACACAGATTTTAAGAGTGAAGGATAACGATGCTCAAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCAc                                                                                                             >  2:299504/1‑150 (MQ=11)

                                          ggTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCAGCACGACCCACCAGTTTTAACATCAAACTCAGATGTAAAGCGTGAAGGATAACGATGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGa                                                                                                           >  2:1640483/1‑150 (MQ=255)

                                            tAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGc                                                                                                                                             >  1:2791274/1‑114 (MQ=255)

                                            tAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGc                                                                                                                                             <  2:2791274/114‑1 (MQ=255)

                                                   gTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGGAGCGAGTCAACCTGCACGACCCACCAAt                                                                                                  >  1:346580/1‑150 (MQ=255)

                                                    ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATc                                                                                                 >  1:165092/1‑150 (MQ=255)

                                                    ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATc                                                                                                 >  1:1797711/1‑150 (MQ=255)

                                                      gACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCAGCACGACCCACCAATCtt                                                                                               >  1:1135601/1‑150 (MQ=255)

                                                       aCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTa                                                                                              >  1:2692550/1‑150 (MQ=255)

                                                         ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTa                                                                                                                                        <  2:2150861/106‑1 (MQ=255)

                                                         ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTa                                                                                                                                        >  1:2150861/1‑106 (MQ=255)

                                                         ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaaa                                                                                            <  2:723861/150‑1 (MQ=255)

                                                          tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCATAAAg                                                                                           >  1:2718059/1‑150 (MQ=255)

                                                             aaTCAATTGGTCGCAGGTTCGAATCCTGCACGTCCCACCAGTTTTAACATCTATCTCTGATGTTAAGCGTTAAGGATATCGTTGCGCGAGGATCGTCCGGTATGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGAtt                                                                                        <  2:2757155/150‑1 (MQ=17)

                                                             aaTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCTCCAGTTTTAACATCAAACTCATATGTTAAGCGTGAAGGATATCGTTGCGCCAGGAACGGCCGGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGAtt                                                                                        <  2:1751920/150‑1 (MQ=17)

                                                              aTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTg                                                                                       >  1:2022421/1‑150 (MQ=255)

                                                               tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTgg                                                                                      >  2:1563704/1‑150 (MQ=255)

                                                                 aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGcc                                                                                    >  2:2000122/1‑150 (MQ=255)

                                                                 aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCAGCACGACCCACCAATCTTAAAGAGTGGcc                                                                                    >  1:2263872/1‑150 (MQ=255)

                                                                 aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCAGCACGACCCACCAATCTGAAAGATTGGcc                                                                                    >  1:113306/1‑150 (MQ=255)

                                                                   ttGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCTAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGcccc                                                                                  <  2:2505059/150‑1 (MQ=255)

                                                                      gTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAg                                                                               >  1:321006/1‑150 (MQ=255)

                                                                      gTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAg                                                                               >  1:984004/1‑150 (MQ=255)

                                                                       tCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGt                                                                              >  1:1233353/1‑150 (MQ=11)

                                                                         gCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaaa                                                                                            >  1:1643282/1‑134 (MQ=255)

                                                                         gCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaaa                                                                                            <  2:1643282/134‑1 (MQ=255)

                                                                         gCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTaa                                                                            >  1:1845614/1‑150 (MQ=255)

                                                                           aGGTTCGAATCCTGCACGACCCACCAGTTTTATCATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGTCGCGAGTCATCCTGCACGACCCACCAAGCTTAAAGATTGGCCCCGGGTaaat                                                                          >  1:1754658/1‑149 (MQ=17)

                                                                           aGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACGCAGAAGTTAAGCGTGAAGGATAACGTGCCGCCGGCAACGGCCCCAGGGGCGGGCGAAGCGAGTAATCCGTCACGACCCCCCCATCTTAAAGATTGGAGCCGGGTaaaa                                                                          >  1:1754690/1‑150 (MQ=17)

                                                                             gTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAAt                                                                        <  1:2333026/150‑1 (MQ=255)

                                                                                 gAATCCTGCAATACCCACTTTTTATTCAATAAAAATCAGATGTTAAGCGATAAGGATAACGTTGCGACACCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCttt                                                                    <  1:117967/150‑1 (MQ=18)

                                                                                   aTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCa                                                                  <  1:712983/150‑1 (MQ=255)

                                                                                      cTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGt                                                               >  1:565839/1‑150 (MQ=255)

                                                                                      cTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGt                                                               >  2:1783654/1‑150 (MQ=255)

                                                                                      cTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGt                                                               >  1:1995972/1‑150 (MQ=255)

                                                                                       tGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTa                                                              >  1:1983779/1‑150 (MQ=255)

                                                                                           cGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAacac                                                          >  1:1279117/1‑150 (MQ=255)

                                                                                               ccaccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGt                                                      >  1:2635426/1‑150 (MQ=255)

                                                                                                  ccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATg                                                   >  2:1776898/1‑150 (MQ=255)

                                                                                                  ccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATg                                                   >  1:789433/1‑150 (MQ=255)

                                                                                                  ccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATg                                                   >  2:1776935/1‑150 (MQ=255)

                                                                                                   caGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATgg                                                  <  2:1233353/150‑1 (MQ=18)

                                                                                                   caGTTTTAACATCCAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATgg                                                  <  2:437513/150‑1 (MQ=255)

                                                                                                    aGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATggg                                                 >  1:530964/1‑150 (MQ=17)

                                                                                                      ttttACCATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTc                                               <  1:2496544/150‑1 (MQ=255)

                                                                                                      ttttAACATCAAACACAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTc                                               <  1:2496559/150‑1 (MQ=255)

                                                                                                        ttAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGt                                             <  2:1775616/150‑1 (MQ=255)

                                                                                                        ttAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGt                                             <  1:1079369/150‑1 (MQ=255)

                                                                                                            cATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAAACATACAGGTAACACCCGTATGGGTCGTTAGc                                         >  1:2791426/1‑150 (MQ=255)

                                                                                                             aTCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCAACCTGCACGACCCACCAAACATAAAGATTGGCCCCGAGAAAAAAACGTAAAGGTAACACACGAAAGGGTCGTTAGca                                        >  1:1931375/1‑149 (MQ=18)

                                                                                                              tCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAAACTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGAATGGGTCGATAGCTc                                       >  1:1377789/1‑150 (MQ=255)

                                                                                                               ccaccTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGACGAGCGACGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCa                                      <  2:1488000/146‑1 (MQ=255)

                                                                                                                gaagacAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAg                                     >  2:2514885/6‑150 (MQ=255)

                                                                                                                  agacAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGtt                                   <  1:2514885/147‑1 (MQ=255)

                                                                                                                    tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTgg                                 >  1:1297577/1‑150 (MQ=255)

                                                                                                                    tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTgg                                 <  1:2748633/150‑1 (MQ=255)

                                                                                                                    tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTgg                                 >  1:382577/1‑150 (MQ=255)

                                                                                                                    tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGGATGGGTCGTTAGCTCAGTTgg                                 >  1:2870851/1‑150 (MQ=255)

                                                                                                                     cAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGt                                <  1:263318/150‑1 (MQ=255)

                                                                                                                     cAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGt                                <  2:2022421/150‑1 (MQ=255)

                                                                                                                      aGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTa                               >  1:643315/1‑150 (MQ=255)

                                                                                                                        aTGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTaga                             >  1:1561127/1‑150 (MQ=255)

                                                                                                                           ttAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCa                          >  2:2731777/1‑150 (MQ=255)

                                                                                                                                 gTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAc                    <  2:2692550/150‑1 (MQ=255)

                                                                                                                                  tgtaGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGt                                <  1:1100113/134‑1 (MQ=255)

                                                                                                                                  tgtaGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGt                                >  2:1100113/4‑137 (MQ=255)

                                                                                                                                  tgtaGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGt                                <  1:1100178/134‑1 (MQ=255)

                                                                                                                                  tgtaGGATAACGTTGCGACAGCAACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGt                                >  2:1100178/4‑137 (MQ=255)

                                                                                                                                  tGACGGATACCGTTGCGCCAGCACCGGCCCGCAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACt                   <  2:2562111/150‑1 (MQ=255)

                                                                                                                                    aaGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACttt                 <  1:1163540/150‑1 (MQ=255)

                                                                                                                                     aGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACtttt                <  2:539333/150‑1 (MQ=255)

                                                                                                                                     aGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACtttt                <  1:2185117/150‑1 (MQ=255)

                                                                                                                                     aGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACtttt                >  1:726436/1‑150 (MQ=255)

                                                                                                                                      ggATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTa               <  2:1797711/150‑1 (MQ=255)

                                                                                                                                       gATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTaa              >  1:110193/1‑150 (MQ=255)

                                                                                                                                       gATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTaa              >  1:2663537/1‑150 (MQ=255)

                                                                                                                                       gATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTaa              >  1:954332/1‑150 (MQ=255)

                                                                                                                                       gATAACGTTGCGACAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTaa              >  1:2875130/1‑150 (MQ=255)

                                                                                                                                           aCGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCaa          >  2:2647527/1‑150 (MQ=255)

                                                                                                                                            cGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGc                                         <  2:2027569/118‑1 (MQ=255)

                                                                                                                                            cGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGc                                         >  1:2027569/1‑118 (MQ=255)

                                                                                                                                             gTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCTCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAACATCCTTCAGGTAACACCCGGATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAtt        <  2:438566/150‑1 (MQ=255)

                                                                                                                                             gTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCCATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAtt        <  2:67805/150‑1 (MQ=255)

                                                                                                                                                gcgcCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGt     <  2:398376/150‑1 (MQ=255)

                                                                                                                                                gcgcCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGt     <  2:398385/150‑1 (MQ=255)

                                                                                                                                                 cgcCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTc    <  1:260533/150‑1 (MQ=255)

                                                                                                                                                  gcCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAATATAGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGGCGTGAGCTCAGATGGTCGAGCAGTGGACTTTTAATCAAATGGGCg   >  1:588508/1‑150 (MQ=37)

                                                                                                                                                  gcCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAAACGATCTGGTCACACCCGAATGGGTCGTTAGCTCAGGTGGTCGAGCAGTTGACTTTTAATACATTGGTCg   >  2:2415478/1‑150 (MQ=37)

                                                                                                                                                   ccAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGGGGGTAGAGCAGTTGACTTTTAAGCAATTGGTCGc  >  1:1637346/1‑150 (MQ=255)

TCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGC  >  NC_000913/781122‑781418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`