Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,466,562	G→A	7.7%	pseudogene (1171/2513 nt)	ydbA →	pseudogene, autotransporter homolog; interrupted by IS2 and IS30

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,466,562	0	G	A	7.7%	148.9 / 2.8	58	pseudogene (1171/2513 nt)	ydbA	pseudogene, autotransporter homolog; interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base G (33/15);  new base A (2/2);  total (36/22)
Fisher's exact test for biased strand distribution p-value = 5.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATT  >  NC_000913/1466416‑1466711                                                                                                                                                   |                                                                                                                                                      tCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTg                                                                                                                                                    >  2:473228/1‑150 (MQ=255) tCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCAAGACCGTCACCGATCCGGAGTCCATCGGGCGCCAGGtgg                                                                                                                                                    >  2:1938136/1‑148 (MQ=255) tCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCCGCGACAGCGCAAAGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTg                                                                                                                                                    >  2:2737085/1‑150 (MQ=255)     cGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACgg                                                                                                                                                <  1:473228/150‑1 (MQ=38)     cGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACgg                                                                                                                                                >  1:120877/1‑150 (MQ=38)            aaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAg                                                                                                                                         >  1:2765889/1‑150 (MQ=39)            aaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAg                                                                                                                                         >  1:2765882/1‑150 (MQ=39)            aaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAg                                                                                                                                         <  2:2598311/150‑1 (MQ=39)            aaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAg                                                                                                                                         <  2:120877/150‑1 (MQ=21)               ggAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCg                                                                                                                                      >  1:2903502/1‑150 (MQ=40)                  aaaGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGccatc                                                                                                                                   >  2:723840/1‑146 (MQ=18)                       tATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTaa                                                                                                                              <  2:1489417/150‑1 (MQ=40)                           cAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTaacaac                                                                                                                          >  1:48430/1‑150 (MQ=255)                             ggACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGa                                                                                                                        <  2:1415291/150‑1 (MQ=255)                             ggACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGa                                                                                                                        >  1:2572853/1‑150 (MQ=255)                                          gATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAgcgtc                                                                                                           >  1:2159342/1‑148 (MQ=255)                                              tCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCa                                                                                                       <  2:1031647/150‑1 (MQ=255)                                                aGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCAcc                                                                                                     >  1:2782581/1‑150 (MQ=255)                                                    gcggcggTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACg                                                                                                 <  1:2906637/150‑1 (MQ=255)                                                    gcggcggTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACg                                                                                                 <  1:39555/150‑1 (MQ=255)                                                      ggcggTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCACTATCACCAAAggc                                                                                               >  2:2326499/1‑149 (MQ=255)                                                          gTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCa                                                                                           <  2:839579/150‑1 (MQ=255)                                                              cGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCgg                                                                                       <  2:48430/150‑1 (MQ=255)                                                                gTATTGATAGCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCa                                                                                     <  2:676438/150‑1 (MQ=255)                                                                        atCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGAtt                                                                             >  2:2683795/1‑150 (MQ=255)                                                                            cgggCGACAGCGGAGCGGTGGATATGTAGGGCACCTTGTCGGTCACGGTTGCGGAGTCCATGGGTATCCAGTTTGACGGCGACCAGGCGGTGGTTAAGAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACg                                                                         <  2:1696739/148‑1 (MQ=17)                                                                             cGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACgg                                                                        >  2:2318074/1‑150 (MQ=255)                                                                              ggCGACAGCGCAACCGTTGATATCAAGGGCACCATGACCGTCTCCGATCCTGTTCGCATCTGTCTACTGTTTGACGGTGACAAGGCCTTAGTCAATAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGt                                                                       <  2:982283/150‑1 (MQ=2)                                                                                  aCAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTAGTGAACAACGAAGGCGAGAGCGCCATCACCAACGGAGGCACCGGCACTCAGATCAACGGtgagg                                                                   >  2:1524379/1‑148 (MQ=14)                                                                                           cGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGTTTGACGGAGACAAGGCGTTTGTTAATAACGAAGGCGAGAGCTCCATCACCAACGGTGGCACCGGCACGCAGATTAAGGGTGATGACGCCACgg                                                          >  1:1100829/1‑150 (MQ=11)                                                                                             gTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCa                                                        >  2:1579581/1‑150 (MQ=255)                                                                                             gTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCa                                                        >  2:2883763/1‑150 (MQ=255)                                                                                                  tAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGAGGACGCCACGGCAaacaa                                                   >  1:2325316/1‑150 (MQ=255)                                                                                                  gcgatctggCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGGTTGACGGCGACAAGGCGGTTGTTAAGAACGAAGGCGAGAGCTCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaacaa                                                   <  2:2310068/143‑1 (MQ=14)                                                                                                     cAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTGGTGAACAACGAAGGCGAGAGCGCGATCACCAAAGGGGGCACCGGCACGCAGATGAACGGTGAGGACGCCACGGCGaagaacag                                                >  1:1636334/1‑149 (MQ=14)                                                                                                      aaGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATAAACGGTGATGACGCCACGGCAaacaacaac                                               >  1:1692624/1‑150 (MQ=255)                                                                                                             ccATGACCCTCACCGATCCGGAGTCCATCGGTATCCAGCTAGACGGCGACCAGGCGGTAGATAACAACGAAGGCGAGAGCGCCAACACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCaaaa                                        <  2:1100829/150‑1 (MQ=17)                                                                                                               aTGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAAGGGAGGCACCGGCACTCAGATAAACGGTGAAGACGCCACCGCGAAAAACAGAGGAAAAacc                                      >  2:2343124/1‑150 (MQ=14)                                                                                                                  aaccacACCGATCATCAGACCATCGGTATCCAGCTAGACGGAGACCAGGCCTTTGTAAAAAACGAAGGCGAGAGCCCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAaccacc                                   <  2:642647/145‑1 (MQ=14)                                                                                                                     gTCACCGATCCGTAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGtt                                <  2:1827275/150‑1 (MQ=255)                                                                                                                     gTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTGGTGAACAACGAAGGCGAGAGCGCCATCACCAACGGAGGCACCGGCACGCAGATAAACGGTGAGGACGCCACCGCCAAAAACACCGGCAAAACCACCGtt                                >  1:602579/1‑150 (MQ=17)                                                                                                                     gTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTAGTGAACAACGAAGGCGAGAGCGCCATCACCAACGGAGGCACCGGCACGCAGATAAACGGTGAAGACGCCACCGCCAAAAACACAGGCAAAACCACAGtt                                >  1:322413/1‑150 (MQ=17)                                                                                                                      tCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCGATCACCAAAGGCGGCACCGGCACGCAGATAAACGGTGAGGACGCCACGGCGAATAACAGAGGAAAAACCACCGTTg                               >  2:2645233/1‑150 (MQ=14)                                                                                                                      tCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTg                               >  2:1036046/1‑150 (MQ=255)                                                                                                                       cACCGATCCGGAGTCCATCGGTATCCAGTTTGACGGTGACAAGGCCTTTGTAAAAAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGTTGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGa                              <  1:1185760/150‑1 (MQ=11)                                                                                                                          cGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACgg                           >  2:2657669/1‑150 (MQ=255)                                                                                                                          cGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACgg                           >  2:2657670/1‑150 (MQ=255)                                                                                                                           gATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGc                          >  2:2364066/1‑150 (MQ=255)                                                                                                                             tCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGAAGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCaa                        >  1:995459/1‑150 (MQ=255)                                                                                                                               cGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAgg                      <  2:216096/150‑1 (MQ=255)                                                                                                                                 gAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATAAACGGTCATGACGCCACCGCAAAAAACAAAGGCAAAACCACCGTTGACGGCAAGGAt                    >  2:2868328/1‑150 (MQ=18)                                                                                                                                    tCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTcc                 >  1:1700385/1‑150 (MQ=255)                                                                                                                                    tCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATAAACGGTGAAGACGCCACCGCAAAAAACAGAGGCAAAACAACCGCACACGGCAAGCATAcc                 >  1:2521866/1‑150 (MQ=18)                                                                                                                                       aTCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAcc              <  2:2903502/150‑1 (MQ=255)                                                                                                                                       aTCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAcc              >  1:2669041/1‑150 (MQ=255)                                                                                                                                       aTCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAcc              >  1:1528133/1‑150 (MQ=255)                                                                                                                                        tCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCg             <  2:2765889/150‑1 (MQ=255)                                                                                                                                        tCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCg             <  2:2765882/150‑1 (MQ=255)                                                                                                                                                   gTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAATGATTCCACCGGTACGGAAAtt  >  1:1514794/1‑150 (MQ=255)                                                                                                                                                   gTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGACAtt  >  2:2589965/1‑150 (MQ=255)                                                                                                                                                   gTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAAtt  >  1:2524243/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                      TCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATT  >  NC_000913/1466416‑1466711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑