Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,234 C→A 7.2% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2340CA 7.2% 382.0 / 3.8 132intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (46/71);  new base A (4/10);  total (51/81)
Fisher's exact test for biased strand distribution p-value = 5.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

GTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177085‑2177383
                                                                                                                                                     |                                                                                                                                                     
     cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGGTTTAGATTTAAAAat                                                                                                                                                  >  2:810254/1‑150 (MQ=255)
         ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATaa                                                                                                                                              <  1:2910096/150‑1 (MQ=255)
         ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATaa                                                                                                                                              <  2:148180/150‑1 (MQ=255)
              cTACCACCACTTGCATCGTTTCGAGAGTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCTGCTTCTGCTTTGTCGATACCAAGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAATATATAATGaaa                                                                                                                                         <  1:2672997/150‑1 (MQ=255)
                  caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTCGATTTCAAAATATAATTAAattat                                                                                                                                     >  1:295995/1‑150 (MQ=255)
                  caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAaatat                                                                                                                                     >  1:295994/1‑150 (MQ=255)
                   accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                    <  1:1163203/150‑1 (MQ=255)
                   accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                    <  1:258865/150‑1 (MQ=255)
                    ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                   <  2:1240423/150‑1 (MQ=255)
                      acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGt                                                                                                                                 <  1:892686/150‑1 (MQ=255)
                      acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGt                                                                                                                                 <  2:2470856/150‑1 (MQ=255)
                       cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtt                                                                                                                                <  1:2738375/150‑1 (MQ=255)
                        ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAAAATAAATAAATTAAtttttt                                                                                                                               >  1:1101404/1‑150 (MQ=255)
                        ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                               <  2:890770/150‑1 (MQ=255)
                          gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTATTTGttttt                                                                                                                             <  1:1730277/150‑1 (MQ=255)
                          gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATAAAAAGAAAATATtttttttt                                                                                                                             >  2:2699579/1‑150 (MQ=255)
                                tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                       <  2:2152970/150‑1 (MQ=255)
                                tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                       >  1:465552/1‑150 (MQ=255)
                                  tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                     <  2:1538940/150‑1 (MQ=255)
                                  tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                     <  2:494316/150‑1 (MQ=255)
                                   cGAGATTGTGAATATTGACTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                    <  2:2679882/150‑1 (MQ=255)
                                   cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAga                                                                                                                    >  1:456140/1‑150 (MQ=255)
                                   cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                    <  2:1112389/150‑1 (MQ=255)
                                    gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATATTTGTTTTTAAATATCgag                                                                                                                   >  1:1633945/1‑150 (MQ=255)
                                    gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAAATATTTTTTTTTAAAAATagag                                                                                                                   >  1:1622943/1‑150 (MQ=255)
                                    gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATGAAATTATTTGTTTTTAAATATCgag                                                                                                                   >  1:2336813/1‑150 (MQ=255)
                                       aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATa                                                                                                                <  2:465370/150‑1 (MQ=255)
                                       aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATa                                                                                                                <  2:900424/150‑1 (MQ=255)
                                           tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATGAAATTATTTGTTTTTAAAAATCGAGATAAcca                                                                                                            >  1:2767797/1‑148 (MQ=255)
                                           tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATAACCTGTCGTGTGTTTTCGATTTCAAAATAAAATGTAATTATTTGTTTTTTAATATAGAGATAACGa                                                                                                            >  1:2342732/1‑150 (MQ=255)
                                            gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                           <  1:1936352/150‑1 (MQ=255)
                                            gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATAGAGATAAAGAt                                                                                                           >  1:2089280/1‑150 (MQ=255)
                                             aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                          <  1:629540/150‑1 (MQ=255)
                                              atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                         <  2:2842126/150‑1 (MQ=255)
                                                 ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTTAAAATCGAGATAACGCTCCCaa                                                                                                      >  1:1119613/1‑150 (MQ=255)
                                                   gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAAATATTTGTTTTTAAATATCGAGATAACGATCACaaaa                                                                                                    >  1:2580832/1‑150 (MQ=255)
                                                       gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                <  2:1333151/150‑1 (MQ=255)
                                                       gCCGGAACCGCATAACCGCCGCGCGGTGCGGTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTGTTCGATTTCAAAATATAAGGCACTGAGTTGTTTTTAAATATCGAGATAACCATCACCCAAACGa                                                                                                >  2:1395993/1‑150 (MQ=255)
                                                         cGGAACCGCATAACCGCCGCGCTGTGCGGTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTACATATCGAGATAACGATCACAAAAACGACa                                                                                              >  2:1891230/1‑150 (MQ=255)
                                                          ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAATATAAAGAAATTATTTTTTTTTAAAAATAGAGAAAAAGAAAAAAAAAAccaaaa                                                                                             >  2:512220/1‑145 (MQ=255)
                                                                 cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAAATATTTTTTTTTTAATATAGAGATAACGATCACAAAAAAGACAAAATTaaa                                                                                      >  1:800329/1‑150 (MQ=255)
                                                                       cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt                                                                                <  2:1093415/150‑1 (MQ=255)
                                                                       cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATAGCCGGTCGTTTGTTTTCGTTTTCAAAATATAATGAAATTATTTGTTTTTAAATTTCGAGATAACGATCACAAAAACGACAATATGAAAattatt                                                                                <  2:820924/150‑1 (MQ=255)
                                                                       cgccgcGCGGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt                                                                                <  2:820947/150‑1 (MQ=255)
                                                                        gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAATTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTc                                                                               <  2:2074796/150‑1 (MQ=255)
                                                                               tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGt                                                                        <  2:2844177/150‑1 (MQ=255)
                                                                                  gCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaa                                                                     <  2:2247010/150‑1 (MQ=255)
                                                                                     ttttacAGCATTTGTTTTGTCGATACAACTTACATTTTCATCTCCTGTGTTTTGTTTTCTATTAAAAAATATAATCAAATTATTTTTTTTTAAATATCGACATAACTATAACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAgg                                                                  <  1:266544/145‑1 (MQ=255)
                                                                                     ttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCTAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAgg                                                                  <  2:557096/150‑1 (MQ=255)
                                                                                     ttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAgg                                                                  <  2:2018454/150‑1 (MQ=255)
                                                                                     ttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAgg                                                                  <  2:1703920/150‑1 (MQ=255)
                                                                                      gacgtaGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGc                                                                 <  2:1870431/145‑1 (MQ=255)
                                                                                            gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAATAAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaa                                                           >  2:1483339/1‑150 (MQ=255)
                                                                                             cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCTAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                          <  2:382674/150‑1 (MQ=255)
                                                                                             cATCTGCTTTCTCCAAACACCCAAAATTTTAATAACCTGTCTTTTGTTTTCTATTAAAAATTATAATTATATTATTTTTTTTTAATTATCGATATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                          <  1:2749214/150‑1 (MQ=255)
                                                                                             aaTCTGCTTTGTCTATACCACTTAATTTTTAATATCCTGTCTTTTTTTTTCTATTTAAAAATATAATTAATTTTTTTTTTTTTAAATATCTATATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                          <  1:2666564/149‑1 (MQ=255)
                                                                                              ttCTGCTTTGCCTATACCTCGTAAATTTTCATATCCTTTTTTTTTTTTTCTTTTTAAAAATATAATCAATTTTTTTTTTTTTAAATATCGATATAACTATAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  1:1562938/149‑1 (MQ=255)
                                                                                              ctCTGGTTTGTCGATACCACGTAGATTTTCTTATCCTGTGGTTTTTTTTCGATTTCTAATTATAATGTAATTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  2:2464422/149‑1 (MQ=255)
                                                                                                cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACgg                                                       <  2:1341510/150‑1 (MQ=255)
                                                                                                  gCTTTGTCTATACCACTTAAATTTTAATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAATTTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGcc                                                     <  2:2707363/150‑1 (MQ=255)
                                                                                                   cTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGTTAACGATCACCAAAACGACAATATGAAAAGTATTCGGGGCGTGAAAGGCAAAAAATAGGCCt                                                    >  2:642269/1‑150 (MQ=255)
                                                                                                    tttGTCGATACCACTTACATTTTCATATCGTGTCGTTTGTTTTCGATTTAAAAATATAATTAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTc                                                   <  2:742412/150‑1 (MQ=255)
                                                                                                    tttGTCGATACCACGTACATTTTCATTTCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTc                                                   <  2:2401318/150‑1 (MQ=255)
                                                                                                    tttGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTc                                                   <  2:2401334/150‑1 (MQ=255)
                                                                                                    tttGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTc                                                   <  2:2401312/150‑1 (MQ=255)
                                                                                                    tttGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGTATTTAAAAAAAAAAATAAATTAATTTTTTTTAAATAAAGAGAAAAAAAACACAAAAAAAACAAAAATAAAAATATTAGAGGGGTGAAATTAAAAAAAAAGGccga                                                   >  1:1653330/1‑148 (MQ=255)
                                                                                                       gTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCg                                                <  2:2290954/150‑1 (MQ=255)
                                                                                                          gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                             <  2:455039/150‑1 (MQ=255)
                                                                                                          gATACCACGTACATTTTCATATCCTGTCGTTTGTGTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                             <  2:1626110/150‑1 (MQ=255)
                                                                                                           aTACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAg                                            <  2:143493/150‑1 (MQ=255)
                                                                                                              ccACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTGAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAAACATCACAAAAAAGACAATATGAAAATTATTAGAGGAGTGGAAGGCAAAAAAACGGCCACCCCCTAGGGa                                         >  2:501075/1‑150 (MQ=255)
                                                                                                                 cttACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGc                                      <  2:1187667/148‑1 (MQ=255)
                                                                                                                  gTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGCGGGAAAGGCAAAAAACGGCCACCCGCTAGGGAAGCCg                                    >  2:597112/1‑150 (MQ=255)
                                                                                                                  gTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAACGACAATAAGAAAATTATTCGAGGAGTGGAAGGCAAAAAAAAGGCCTCCCGATAGGGAAGcc                                     >  2:2087240/1‑150 (MQ=255)
                                                                                                                   tACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCg                                    >  2:1525184/1‑150 (MQ=255)
                                                                                                                    aCATTTTCATATCCTGTCGTTTTTTTTCTATTTCAAAATATAATTAATTTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCGt                                   <  2:1227462/150‑1 (MQ=255)
                                                                                                                          tCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                             <  2:698310/150‑1 (MQ=255)
                                                                                                                          tCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                             <  2:698312/150‑1 (MQ=255)
                                                                                                                             tagccTGTCTTTTGTTTTCTATTTCAAAATATAATGAAATTTTTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTACCGATAGGGAAGCCGTAGCAAAGTg                          <  1:2126531/147‑1 (MQ=255)
                                                                                                                                  tGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAAGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                     >  2:2547968/1‑150 (MQ=255)
                                                                                                                                  tGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAAGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                     >  2:2205541/1‑150 (MQ=255)
                                                                                                                                    tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAAAATGAAAATTATTCGAGGAGTGAAAGGAAAAAAAACGGCCACCCGATAGGGAACCCGTAGAAAAGGGCCCgggt                   >  2:2434374/1‑150 (MQ=255)
                                                                                                                                        ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt               >  1:2719828/1‑150 (MQ=255)
                                                                                                                                        ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt               >  1:2719760/1‑150 (MQ=255)
                                                                                                                                          gtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             <  2:2551823/150‑1 (MQ=255)
                                                                                                                                          gtttTAGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             <  2:1547853/150‑1 (MQ=255)
                                                                                                                                           ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGACAGGCAAAAAAACCGCCTCCCGATCGGGAAGCCGACGCAAAGTGCGCGTGTGTTTATg            >  2:1014560/1‑150 (MQ=255)
                                                                                                                                           ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGACGCCGTAGCAAAGTGCGCGTGTTTTTATg            >  2:1014563/1‑150 (MQ=255)
                                                                                                                                            tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCACAGTGCGCGTGTTTTTATGc           >  2:1367584/1‑150 (MQ=255)
                                                                                                                                            tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc           >  1:2196100/1‑150 (MQ=255)
                                                                                                                                            tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc           >  2:1367598/1‑150 (MQ=255)
                                                                                                                                             ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc          >  1:1513079/1‑150 (MQ=255)
                                                                                                                                              tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg         >  1:2172652/1‑150 (MQ=255)
                                                                                                                                              tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg         >  1:2439416/1‑150 (MQ=255)
                                                                                                                                              gcGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATACCGAGCACAACAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCGCCCGATAGGGAAGCCGTAGCAACGTGCGCGTGTTTTTATGCCg         <  2:1119613/149‑1 (MQ=255)
                                                                                                                                                   ttCAAAATATAATGAAATTATTTGTTTTTGAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAGAGGGACGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc    >  2:1814351/1‑150 (MQ=255)
                                                                                                                                                    tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg   >  2:570890/1‑150 (MQ=255)
                                                                                                                                                    acAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACACAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCGCCCGATAGGGAAGCCGTTGCAAAGTGCGCGTGTTTTTATGCCGGATGCg   <  2:2303821/149‑1 (MQ=255)
                                                                                                                                                     cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  <  2:2756401/150‑1 (MQ=255)
                                                                                                                                                     cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  <  1:393180/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177085‑2177383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: