Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,304,522 G→T 5.4% intergenic (+129/+586) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,5220GT 5.4% 683.4 / 15.3 204intergenic (+129/+586)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base G (77/114);  new base T (6/6);  total (84/120)
Fisher's exact test for biased strand distribution p-value = 5.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

GACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCAT  >  NC_000913/2304373‑2304671
                                                                                                                                                     |                                                                                                                                                     
 aCAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCgg                                                                                                                                                      <  1:1518401/150‑1 (MQ=255)
   aaCGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCa                                                                                                                                                    >  2:395257/1‑150 (MQ=255)
     cgcgGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCAcc                                                                                                                                                  <  2:1196314/150‑1 (MQ=255)
      gcgGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCAccc                                                                                                                                                 >  1:866442/1‑150 (MQ=255)
       cgGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCAcccc                                                                                                                                                >  2:1457668/1‑150 (MQ=255)
        ggTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCg                                                                                                                                               <  2:64015/150‑1 (MQ=255)
           aGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAg                                                                                                                                            <  2:2175296/150‑1 (MQ=255)
             ttCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGt                                                                                                                                          >  2:800282/1‑150 (MQ=25)
             ttCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGt                                                                                                                                          <  1:228052/150‑1 (MQ=255)
               cGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCa                                                                                                                                        <  1:1829568/150‑1 (MQ=255)
               cGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGACCGAGCAGGATGCCACATCAGACCAGCGCGCGGAGAAGCAGGCCTACAAAGCTGTGATGTGCTCCAACACGTAGGtgg                                                                                                                                        >  1:121841/1‑148 (MQ=2)
                  tAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGAc                                                                                                                                     >  1:51233/1‑150 (MQ=255)
                  tAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACCCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTcggcc                                                                                                                                     >  1:504079/1‑146 (MQ=255)
                       tGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGc                                                                                                                                <  1:1651765/150‑1 (MQ=255)
                       tGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGc                                                                                                                                <  1:1933261/150‑1 (MQ=255)
                            tGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCTGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCa                                                                                                                           <  2:604400/150‑1 (MQ=255)
                            tGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCa                                                                                                                           <  2:199138/150‑1 (MQ=40)
                                      cACCCCGTAGGTCAGACTTGGCGGTCACGCCGCATCCGACATCCTACGCCCGAGCCGGTTGCCTTATGCTACTCTGGCTCGTATTTTCAGGCCTACACCGCTTTGAAGTGCGGCCCCCCGTAGGTCAGACAAGGCGGTCACGCCGCATcc                                                                                                                 <  2:1508596/150‑1 (MQ=14)
                                      cACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATcc                                                                                                                 <  1:75831/150‑1 (MQ=255)
                                        ccccGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTTAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGa                                                                                                               <  2:1869995/150‑1 (MQ=255)
                                        ccccGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGa                                                                                                               <  1:2508991/150‑1 (MQ=255)
                                         cccGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGGAGCGACGCTGGCGCGGCGTAACAGGCCTACACCGCGGTGACGGGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGAc                                                                                                              >  1:2546955/1‑150 (MQ=14)
                                               ggTCAGACAAGGCGGTCACGCCTCATCCGACATCCTACGCCCGAGCCGGTTGCCTGATGCGAATCTGTCTCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCaa                                                                                                        <  1:960743/150‑1 (MQ=14)
                                                gTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAAc                                                                                                       <  2:2541786/150‑1 (MQ=255)
                                                 tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACg                                                                                                      <  1:255985/150‑1 (MQ=255)
                                                   aGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGcc                                                                                                    <  1:2174570/150‑1 (MQ=255)
                                                    gACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGACAACACCGCTGTGAACAGCGCCACCCCGTAGGTCAGACAAGGCGGTAACGCCGCATCCGACATCCCACGccc                                                                                                   >  1:561882/1‑150 (MQ=11)
                                                         ggCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGcc                                                                                              >  1:1697436/1‑150 (MQ=255)
                                                             gTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGtt                                                                                          <  1:111853/150‑1 (MQ=255)
                                                               cACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGc                                                                                        <  1:1165422/150‑1 (MQ=255)
                                                                  gccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCGGGCGCGTCGTGTCGGGCCGACACCGCGGGGCAGTGCCGCCCCCCCGGGGGCGGACCAGGCGGGCACGCCGCATCCGCCCGCCCCCGCCCGGGCCGGGTGGCTg                                                                                     >  1:904706/1‑150 (MQ=2)
                                                                             cATCCAACGCCCGAGCCGGTTGCCTGATGCGACTCTGGCGCGTCTTATCAGGCCTACACAGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTg                                                                          <  2:1252005/150‑1 (MQ=255)
                                                                             cATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTg                                                                          <  2:1317236/150‑1 (MQ=255)
                                                                               tacAACGCGCGTGTGGGTTGCCTGATGCTACTCGGTCGCGTCTTATGTTGCCTACTCCGCTGTGAAGTGCGGCACACCGTAGGTCATACAAGGCTGTCACGCCGCATCCGACATCCATCGCCAGAGCCGGTTGACTGATTCGACgattgc                                                                        <  2:1631095/148‑6 (MQ=12)
                                                                                  aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGACGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGcgcg                                                                     >  2:2139254/1‑150 (MQ=255)
                                                                                     gCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt                                                                  <  2:866442/150‑1 (MQ=255)
                                                                                       ccGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTa                                                                <  2:1697436/150‑1 (MQ=255)
                                                                                          aGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGGTGCCTGAGGCGACGCTGGCGCGTCTTATCa                                                             >  2:410375/1‑150 (MQ=255)
                                                                                              ggTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGcc                                                         <  1:395269/150‑1 (MQ=255)
                                                                                               gTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCAACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCt                                                        >  1:28987/1‑150 (MQ=255)
                                                                                                ttGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCCGACAAGGCGGTCCCGCCGCATCCGACATCCAACGCCCGAGCCCGAACCCTGATGCGACGCTGGCGCGTCTTACCAGGCCTa                                                       >  2:1616196/1‑150 (MQ=14)
                                                                                                 tGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGCTCTTAAGTGCGGCAACCAGTATGTCAGACAAGGAGGTTACGCCGCTTCCGACATCCAACGCCCGAGCCGGTTGCCTGAGGCGACGCTGGCGCGTCTTATCAggggtgg                                                      >  2:805969/1‑145 (MQ=14)
                                                                                                    cTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAcc                                                   <  2:2908820/150‑1 (MQ=255)
                                                                                                     tGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCg                                                  <  1:1935418/150‑1 (MQ=255)
                                                                                                      gATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGc                                                 <  2:685487/150‑1 (MQ=255)
                                                                                                      gATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGc                                                 <  2:2332864/150‑1 (MQ=255)
                                                                                                          cGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCAGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtgtg                                             <  1:2716541/150‑1 (MQ=255)
                                                                                                           gACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAAc                                                                                                       <  2:2517015/91‑1 (MQ=255)
                                                                                                           gACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGa                                            >  2:1820196/1‑150 (MQ=255)
                                                                                                           gACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGa                                            >  2:1377966/1‑150 (MQ=255)
                                                                                                            aCGCTGGCGCGTCTTATCAGGACTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGaa                                           <  2:51233/150‑1 (MQ=255)
                                                                                                              gCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGt                                         >  1:2734597/1‑150 (MQ=255)
                                                                                                                  gcgcGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTc                                     <  2:551495/150‑1 (MQ=255)
                                                                                                                   cgcgTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTcc                                    >  1:717901/1‑150 (MQ=255)
                                                                                                                    gcgTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCa                                   >  2:2877782/1‑150 (MQ=255)
                                                                                                                    gcgTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCa                                   >  1:1925069/1‑150 (MQ=255)
                                                                                                                       tCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCGGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGgc                                                                        <  2:1332665/110‑1 (MQ=255)
                                                                                                                       tCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGgc                                                                        >  1:1621834/1‑110 (MQ=255)
                                                                                                                        cTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCAAACGCCCGAGCCGGTTGCCTGATCCGACGCTGGCGCGTCTTATCAGGCCTAAAACGCTGTGAAGTGCTCCAcccc                               >  1:1873741/1‑150 (MQ=255)
                                                                                                                            tCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCg                                                  >  1:124667/1‑127 (MQ=12)
                                                                                                                            tCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAg                           <  1:1869028/150‑1 (MQ=255)
                                                                                                                            tCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAg                           <  2:2803437/150‑1 (MQ=255)
                                                                                                                             cAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAgg                          <  1:806584/150‑1 (MQ=255)
                                                                                                                                gCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTg                                        >  2:614409/1‑133 (MQ=39)
                                                                                                                                gCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCAAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTg                                        <  1:614409/133‑1 (MQ=255)
                                                                                                                                gCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCAGCATCCGACATCCAACGCCCGAGACGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCAGTGAAGTGCTCCACCCCGTAGGttg                       >  1:464951/1‑148 (MQ=255)
                                                                                                                                gCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGACGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCg                       >  1:1653748/1‑150 (MQ=255)
                                                                                                                                  cTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCTCCCCGTAGGTCGGa                     >  1:1365707/1‑150 (MQ=255)
                                                                                                                                  cTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGa                     <  1:1150269/150‑1 (MQ=255)
                                                                                                                                  cTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGa                     <  1:1013431/150‑1 (MQ=255)
                                                                                                                                    acacCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTTGGAAGTGCTCCACCCCGAAGGTCGGATa                   >  1:119181/1‑150 (MQ=255)
                                                                                                                                    acacCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATa                   >  2:207176/1‑150 (MQ=255)
                                                                                                                                     cacCGCTGTGAAGTGCGGCACCCCGTAGGCCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATaa                  <  2:495488/150‑1 (MQ=255)
                                                                                                                                      acCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAg                 <  2:1421840/150‑1 (MQ=255)
                                                                                                                                       ccGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAgg                <  1:1339580/150‑1 (MQ=255)
                                                                                                                                       ccGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAgg                <  1:938104/150‑1 (MQ=255)
                                                                                                                                        tgCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAg                                                            <  1:852842/104‑1 (MQ=14)
                                                                                                                                        cGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAg                                          >  2:108599/1‑123 (MQ=33)
                                                                                                                                        cGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAg                                          <  1:108599/123‑1 (MQ=33)
                                                                                                                                        cGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGGAGGTCGGATAAGGc               >  1:2142952/1‑150 (MQ=255)
                                                                                                                                         gCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCTAACGCCCAAGCCGGTTg                                                                                         <  1:1295111/75‑1 (MQ=255)
                                                                                                                                         gCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCg              <  2:186865/150‑1 (MQ=255)
                                                                                                                                         gCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCg              <  1:2316544/150‑1 (MQ=255)
                                                                                                                                         gCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCg              <  1:395142/150‑1 (MQ=255)
                                                                                                                                         gCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTAACGCCGCATCCGACATCAAACGCCCAAGCCGGTTGCCTGAGGCGACGCGGGCGCGTCTTATCAGGCCTACGCCGCTGTGGAGTGCTCCACCCCGAGGGTCGGATTAGGCg              >  2:2107986/1‑150 (MQ=14)
                                                                                                                                         gCTCTGAAGTGCTGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCg              <  1:313265/150‑1 (MQ=255)
                                                                                                                                          cTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCgg             <  1:1435103/150‑1 (MQ=40)
                                                                                                                                           tgtgAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGt            >  1:2345229/1‑150 (MQ=40)
                                                                                                                                           tgtgAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGt            <  2:1043853/150‑1 (MQ=40)
                                                                                                                                           cgtgAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGt            >  1:1851827/2‑150 (MQ=38)
                                                                                                                                             tgAAGTGCGGCACCCCGTAGGTCAGACAAGGCGTTCACTCCGCATCCGACATCCAACGCCCGAGCGGCTTGCCTGATGCGACGCTGGCGCGTCTTATGAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTa          <  1:141092/150‑1 (MQ=14)
                                                                                                                                             tgAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTa          <  1:2770949/150‑1 (MQ=40)
                                                                                                                                             tgAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGtt                        <  2:1920011/136‑2 (MQ=21)
                                                                                                                                              gAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGt            >  2:380462/1‑147 (MQ=39)
                                                                                                                                              gAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGt            <  1:148685/147‑1 (MQ=39)
                                                                                                                                               aaGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcg        >  2:260898/1‑150 (MQ=255)
                                                                                                                                                aGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgc       <  2:569112/150‑1 (MQ=255)
                                                                                                                                                 gTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgcc      <  2:1550445/150‑1 (MQ=255)
                                                                                                                                                  tGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTAcgccg     <  2:2190776/150‑1 (MQ=255)
                                                                                                                                                   gCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAgg                <  2:309424/138‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCAT  >  NC_000913/2304373‑2304671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: