BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,411,263	A→C	11.9%	A30A (GCT→GCG)	yfbS ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,411,263	0	A	C	11.9%	394.2 / 13.2	154	A30A (GCT→GCG)	yfbS	putative transporter
Reads supporting (aligned to +/- strand): ref base A (83/49); new base C (2/20); total (85/69)
Fisher's exact test for biased strand distribution p-value = 2.29e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 169 total aligned reads displayed.

CCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGA  >  NC_000913/2411114‑2411411

cccATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAAcaaacaaa                                                                                                                                                      <  2:2340471/150‑1 (MQ=255)

cccATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAAcaaacaaa                                                                                                                                                      <  1:944702/150‑1 (MQ=255)

  cATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACCACGTTAGGATCAGAAAAGCCGGACAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGCCAC‑TAACACACAACGc                                                                                                                                                    >  2:992541/1‑150 (MQ=255)

  cATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGc                                                                                                                                                    >  2:1455819/1‑150 (MQ=255)

  cATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGc                                                                                                                                                    >  2:1455757/1‑150 (MQ=255)

   aTTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCg                                                                                                                                                   >  1:1335608/1‑150 (MQ=255)

   aTTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCg                                                                                                                                                   >  1:1335212/1‑150 (MQ=255)

    ttACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGa                                                                                                                                                  <  2:1263159/150‑1 (MQ=255)

    ttACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGa                                                                                                                                                  <  2:2742360/150‑1 (MQ=255)

     tACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGAc                                                                                                                                                 <  2:898399/150‑1 (MQ=255)

      aCGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCTACAA‑TAACAAACAAAGCGAcc                                                                                                                                                <  2:2788035/150‑1 (MQ=255)

      aCGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGAcc                                                                                                                                                <  2:2591598/150‑1 (MQ=255)

         gTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCa                                                                                                                                             >  1:959418/1‑150 (MQ=255)

         gTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCa                                                                                                                                             >  2:803867/1‑150 (MQ=255)

              aaCACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCAt                                                                                                                                        >  2:2509515/1‑150 (MQ=255)

                 acCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACg                                                                                                                                     >  1:895150/1‑150 (MQ=255)

                 acCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACg                                                                                                                                     >  1:2747355/1‑150 (MQ=255)

                      tACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCActc                                                                                                                                >  2:867974/1‑150 (MQ=255)

                           aCCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGccc                                                                                                                           >  1:1033163/1‑150 (MQ=255)

                           aCCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGccc                                                                                                                           >  1:1033096/1‑150 (MQ=255)

                           aCCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGccc                                                                                                                           >  1:165904/1‑150 (MQ=255)

                              aaaCCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTc                                                                                                                        >  2:465967/1‑150 (MQ=255)

                               aaCCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCg                                                                                                                       >  2:737900/1‑150 (MQ=255)

                                 ccATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCa                                                                                                                     >  1:951490/1‑150 (MQ=255)

                                  cATCGCAAATAATAAACAAGGCGGAAATCAGGACAAGTTTTGGATCATAAAAGCGGTAAAATACTTGTGGGACTTTCTGCGTTCCGCTTAATGAAAACGCTACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCaa                                                                                                                    <  2:554862/150‑1 (MQ=255)

                                     cGCCAATAATAAACATGGCGGCAATCAGGACGACGTTAGTATCTGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAACCAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACa                                                                                                                 <  2:2849358/150‑1 (MQ=255)

                                     cGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACa                                                                                                                 >  2:359869/1‑150 (MQ=255)

                                     cGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACa                                                                                                                 >  2:359867/1‑150 (MQ=255)

                                      gCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACaa                                                                                                                >  2:1236777/1‑150 (MQ=255)

                                       ccAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAg                                                                                                               >  1:2054850/1‑150 (MQ=255)

                                       ccAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAg                                                                                                               >  2:1192126/1‑150 (MQ=255)

                                            aataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGAt                                                                                                          >  2:1462219/1‑150 (MQ=255)

                                             ataaCCACGGCGGCAATCCGGACAAGGTTTGGATCAGAAAAGCCGGAAATTAGTTGTGGGACTGTCAGCGTTCCACTTAATGCAAACGCGACAT‑TAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATg                                                                                                         <  2:1527203/150‑1 (MQ=255)

                                                     ggcggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGc                                                                                                 >  1:533606/1‑150 (MQ=255)

                                                      gcggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGcc                                                                                                >  1:1233478/1‑150 (MQ=255)

                                                      gcggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGcc                                                                                                >  1:1839471/1‑150 (MQ=255)

                                                           aaTCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTaa                                                                                           <  2:2376810/150‑1 (MQ=255)

                                                             tCAGGACACGGTTTGGATCAGAAACGCGGGAAATTACTTGTGGGAGTGTCCGCGTTCCGCTTACTGCAACGGGGACAA‑TAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACACGACGATGGCAACGGCCAGTAATg                                                                                         <  2:1758506/150‑1 (MQ=255)

                                                             tCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGAAATg                                                                                         >  1:1994556/1‑150 (MQ=255)

                                                              ctggACAACTTTAGTATCATAAAAGCCGTAAAATACTTGTGGGACTGTCCGGGTCCCGCTTAATGAAAACGCTAAAA‑TAAAAAACACCGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGa                                                                                        <  2:1055349/148‑1 (MQ=255)

                                                              cAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGa                                                                                        <  2:1936926/150‑1 (MQ=255)

                                                               aGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGaa                                                                                       <  2:2417605/150‑1 (MQ=255)

                                                               aGGACAACGTTAGGATCACAAAAGCCGTAAAATACTCCTGGGACTGTCAGCTTTCCGCTTAATGAAAACGCCAAAA‑TAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGaa                                                                                       <  2:1806404/150‑1 (MQ=255)

                                                                 gACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                     >  2:639707/1‑150 (MQ=255)

                                                                 gACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                     >  1:1250300/1‑150 (MQ=255)

                                                                 gACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                     >  1:1250418/1‑150 (MQ=255)

                                                                 gACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                     >  1:2176119/1‑150 (MQ=255)

                                                                 gACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                     >  1:2398585/1‑150 (MQ=255)

                                                                   cAACGTTAGGATCATAAAAGCGGTAAAATACTTCTGGGAGTGTCAGCGTTCCGATTAATGAAAACGCTAAAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGaa                                                                                   <  2:104945/150‑1 (MQ=255)

                                                                   cAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGaa                                                                                   >  1:2098093/1‑150 (MQ=255)

                                                                       gTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCa                                                                               >  2:1334399/1‑150 (MQ=255)

                                                                       gTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCa                                                                               >  2:1885583/1‑150 (MQ=255)

                                                                        ttAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCACTTAATGCAAACGCGAAAA‑TAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCaa                                                                              <  2:1208699/150‑1 (MQ=255)

                                                                            gATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCa                                                                          >  1:2761354/1‑150 (MQ=255)

                                                                             atcttaaaaGCGGGAAATTAGTTGTGGGAGTGTCTGGGTTCCGCTTAATGCAACGGCTAAAT‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGTCGATGGCAACGGCCAGTAATGAAAGAACCCAAATCaa                                                                         <  2:1818655/145‑1 (MQ=255)

                                                                              tCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAt                                                                        >  2:2042699/1‑150 (MQ=255)

                                                                               cAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAtt                                                                       >  1:1829631/1‑150 (MQ=255)

                                                                                  aaaaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAc                                                                    >  2:2225705/1‑150 (MQ=255)

                                                                                      tccGTAAAATTCTTCTGTTACTGTCAGCGTACGTATTATTGAAAACGCTAAAA‑TAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGtt                                                                <  1:2394593/149‑1 (MQ=255)

                                                                                      gCCGTAAAATACTTGTGGGAGTGTCAGGTTTCCGATTATTGAAAACGCTAAAA‑TAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGtt                                                                <  1:1501041/150‑1 (MQ=255)

                                                                                      cagtaaaaaTACTTCTGGTACTTTAAGCGTTCCTATTAATTAAAACGAGACAA‑TAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGtt                                                                <  2:1580040/145‑1 (MQ=255)

                                                                                          gAAAATACTTCTGGGACTGTCTGCGTTCCGATTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTcaca                                                            <  1:423691/150‑1 (MQ=255)

                                                                                            aattACTTCTGGGACTGTCTGCGTTCGGCTTAATGAAAACGCTACAA‑TAACAAACACAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAAc                                                          <  2:790519/147‑1 (MQ=255)

                                                                                                  ttCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCCAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCCATTCACCGTTCACAACGGATcc                                                    >  1:2077857/1‑150 (MQ=255)

                                                                                                  ttCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATcc                                                    >  1:1041954/1‑150 (MQ=255)

                                                                                                  ttCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATcc                                                    >  1:1042109/1‑150 (MQ=255)

                                                                                                   tCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATcgt                                                   >  1:499784/1‑148 (MQ=255)

                                                                                                   tCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCt                                                   >  1:819930/1‑150 (MQ=255)

                                                                                                    cTGGTACTGTCTGCGTTCCGATTAATGAAAACGCTAAAA‑TAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCtt                                                  <  1:598316/150‑1 (MQ=255)

                                                                                                      ggtagtGTCTGCGTCACTCTTAATGC‑ACCGCGGAAATTAAAAAAAAAAGCGACCGCATCCATACGCACTCTGGCCGTCGCAAACAAGACGATGGCAACGGCCAGTTATGAAAGAACCCAAATCAATTCACCGTTCAGAAGTTATCCTTGt                                                <  2:298498/145‑1 (MQ=255)

                                                                                                        gACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTa                                              >  1:19802/1‑150 (MQ=255)

                                                                                                           tGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAAtt                                           >  1:1370268/1‑150 (MQ=255)

                                                                                                             tCAGCTTTCCGCTTAATCAAAACGCTAAAA‑AAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGa                                         <  1:547806/150‑1 (MQ=255)

                                                                                                             tCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGa                                         <  2:2137887/150‑1 (MQ=255)

                                                                                                             tCAGCGTTCCGCTTAATGCAAACGCGAAAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGa                                         <  1:1205303/150‑1 (MQ=255)

                                                                                                             tCAGCGTTCCGATTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGa                                         <  1:118300/150‑1 (MQ=255)

                                                                                                              cAGCTTGCCGCTTAATCAAAACGAGAAAA‑AAACAAACACCGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAg                                        <  1:764118/150‑1 (MQ=255)

                                                                                                                gCGTTCCGATTAATGCAACCCCTACAA‑AAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggg                                      <  2:798608/150‑1 (MQ=255)

                                                                                                                  tttcctattAATCAAAACGCTAAAA‑TAAAAAAAAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggggg                                    <  2:819982/143‑1 (MQ=255)

                                                                                                                      cGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGa                                >  2:2195950/1‑150 (MQ=255)

                                                                                                                        cTTAATGCAAACGCTACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACt                              <  2:1116998/150‑1 (MQ=255)

                                                                                                                        cTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACt                              >  2:2817948/1‑150 (MQ=255)

                                                                                                                        cTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACt                              >  2:2616148/1‑150 (MQ=255)

                                                                                                                        cTTAATGCAAACGCGAAAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACt                              <  2:2540311/150‑1 (MQ=255)

                                                                                                                             tGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAAGTGAGGGGGATGACTTGAtt                         >  2:2277616/1‑150 (MQ=255)

                                                                                                                                aaaCGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTCCTTGAGGGGGATGACTTGATTCTg                      >  2:2724353/1‑150 (MQ=255)

                                                                                                                                aaaCGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTg                      >  1:2744449/1‑150 (MQ=255)

                                                                                                                                 aaCGCTACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGc                     <  2:2792115/150‑1 (MQ=255)

                                                                                                                                 aaCGCGACAA‑TAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGc                     <  1:650028/150‑1 (MQ=255)

                                                                                                                                   cgcgACAA‑TAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCa                   <  2:2054850/150‑1 (MQ=255)

                                                                                                                                   cgcgACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCa                   <  1:157363/150‑1 (MQ=255)

                                                                                                                                      gACAA‑TAACAACCCACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaa                <  2:2713143/150‑1 (MQ=255)

                                                                                                                                              acaaacaaAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGcc         <  2:234586/150‑1 (MQ=255)

                                                                                                                                              acaaacaaAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCGTGTTAATGGAGGGGGATGACTTGATTCTGCCAGAAAAAAGcc         >  1:1991322/1‑150 (MQ=255)

                                                                                                                                               caaacacaGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGccc        <  1:2877271/150‑1 (MQ=255)

                                                                                                                                               caaacaaaGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGccc        <  1:1493869/150‑1 (MQ=255)

                                                                                                                                               aaaacatcGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGccc        <  1:2720055/149‑1 (MQ=255)

                                                                                                                                                 aacaaaGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGCGGGGGATGACTTGATTCTGCCAGAAAAAAGCCCcg      >  2:1434098/1‑150 (MQ=255)

                                                                                                                                                   caacGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgac    <  2:2519631/150‑1 (MQ=255)

                                                                                                                                                   caaaGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgac    <  2:2005280/150‑1 (MQ=255)

CCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAA‑TAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGA  >  NC_000913/2411114‑2411411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`