BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,817,916	T→G	9.5%	intergenic (‑56/+143)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,817,916	0	T	G	9.5%	460.8 / 40.1	158	intergenic (‑56/+143)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base T (72/71); new base G (6/9); total (78/80)
Fisher's exact test for biased strand distribution p-value = 5.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.42e-01

Only 100 of 167 total aligned reads displayed.

GAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCA  >  NC_000913/2817767‑2818065

 aaGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAtt                                                                                                                                                       <  2:1996866/150‑1 (MQ=255)

 aaGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAtt                                                                                                                                                       <  1:2057134/150‑1 (MQ=255)

          gagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGG                                                                                                                                              <  1:1099408‑M1/150‑1 (MQ=255)

          gagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGG                                                                                                                                              <  1:1633269‑M1/150‑1 (MQ=255)

           cgaaTATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGT                                                                                                                                             <  2:853249‑M1/149‑1 (MQ=255)

           agaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGT                                                                                                                                             <  2:1425870‑M1/150‑1 (MQ=255)

           agaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGT                                                                                                                                             <  2:1128242‑M1/150‑1 (MQ=255)

           agaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGT                                                                                                                                             <  2:1091549‑M1/150‑1 (MQ=255)

            gaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTG                                                                                                                                            <  1:2912151‑M1/150‑1 (MQ=255)

                atGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATC                                                                                                                                        >  1:1306196‑M1/1‑150 (MQ=255)

                atGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATC                                                                                                                                        >  1:1459856‑M1/1‑150 (MQ=255)

                   gTGCATCCGGGAGGATTCGAAGCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATGCGG                                                                                                                                     <  2:1753211‑M1/150‑1 (MQ=255)

                   gTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCCACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGG                                                                                                                                     >  1:428294‑M1/1‑150 (MQ=255)

                     gCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGA                                                                                                                                   <  1:2515647‑M1/150‑1 (MQ=255)

                       aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGG                                                                                                                                 <  1:498959‑M1/150‑1 (MQ=255)

                        tCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGA                                                                                                                                >  2:296982‑M1/1‑150 (MQ=255)

                         ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGAT                                                                                                                               <  1:2541968‑M1/150‑1 (MQ=255)

                             gaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACt                                                                                                                           >  1:2845652/1‑150 (MQ=255)

                                    cGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTg                                                                                                                    <  1:1788404/150‑1 (MQ=255)

                                    cGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTg                                                                                                                    <  2:2395090/150‑1 (MQ=255)

                                     gAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGc                                                                                                                   >  2:1258640/1‑150 (MQ=255)

                                       aCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCgg                                                                                                                 <  1:687830/150‑1 (MQ=255)

                                          tCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTc                                                                                                              <  1:1881871/150‑1 (MQ=255)

                                            cGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATACAACTGCAGATATTGATACCGCTACAAAATCCATAACAATTAAGAGATGTAGCATCAGGGAGGATTagaacaaccgaccgctcg                                                                                                            >  2:409515‑M1/1‑132 (MQ=255)

                                                   ccGGTTCGTAGCCGAGTACTCTCTGCAGCTGAGATACGGATGCATCGGGAAAACTTATTCTACTGCTGATCTTGATACCGCTACTACAGCCATATCAATTAAGAGATGGTGCATCAGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCg‑g                                                                                                    <  2:2026108/149‑1 (MQ=17)

                                                      gTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTAATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGAATCCGGGAGGATTAAACGCCTTGCGGCTCGCACATCG‑GGcc                                                                                                 >  2:1193053/1‑150 (MQ=17)

                                                        tCGTATCCGAGAACTCTATCCATCTGATATAAGGATTCATCGCTAAAACTTATACTACTGATTATAATGATACCTATACTAAAGCCATAAAAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGt                                                                                               <  2:1535281/150‑1 (MQ=17)

                                                        tCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGt                                                                                               <  1:1605342/150‑1 (MQ=255)

                                                         cGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGtt                                                                                              <  1:282765/150‑1 (MQ=255)

                                                             gCCGAGTACTCTTTGCCGCTGAGCTACGGATGCATCTGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAACGCCATATCAATTAATAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTa                                                                                          <  2:2593296/150‑1 (MQ=17)

                                                               cGAGTACTCTATCCCGCTGAGCTACGGATGCATCGGGAAAACTTAGTCTCCTGCTGATATTGAGACCGCTACTAACGCCATATCAATTAAGAGATGGTGCCTCCGGGAGGAGTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTaaa                                                                                        <  2:719472/150‑1 (MQ=17)

                                                                 aGTACTCTATCCAGCTGAGCTACGGATGCATCGTGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCtttagc                                                                                      <  1:391599/150‑6 (MQ=255)

                                                                    aCTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAAc                                                                                   <  1:2275967/150‑1 (MQ=255)

                                                                      tctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGt                                                                                 >  2:2547376/1‑150 (MQ=255)

                                                                      tctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGt                                                                                 >  2:2852473/1‑150 (MQ=255)

                                                                       ctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTTCTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTTAAGCAACGtt                                                                                <  1:544572/150‑1 (MQ=255)

                                                                        tATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTa                                                                               >  2:805779/1‑150 (MQ=255)

                                                                        tATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTa                                                                               >  2:1342639/1‑150 (MQ=255)

                                                                          tCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAATGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACACCCCAGGCCGCTC‑CGCTTGGTGGCCGTTGCTAAATCCCCGTTatc                                                                             >  1:1793469/1‑150 (MQ=14)

                                                                          tCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAACGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTatc                                                                             <  1:876799/150‑1 (MQ=255)

                                                                          tCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTatc                                                                             >  1:1143986/1‑150 (MQ=255)

                                                                          tCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTatc                                                                             >  2:1704439/1‑150 (MQ=255)

                                                                          tCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTatc                                                                             <  2:223150/150‑1 (MQ=255)

                                                                          tCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGATGCTAAAGCAACGTTatc                                                                             >  2:430442/1‑150 (MQ=255)

                                                                          tCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCATGCGGCTCGCCATTCG‑GGCCGTTGCTAAATCAACGTTatc                                                                             >  1:1793488/1‑150 (MQ=255)

                                                                             aGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTatcatc                                                                          >  1:2353013/1‑150 (MQ=255)

                                                                             aGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTatcatc                                                                          >  1:2352882/1‑150 (MQ=255)

                                                                             aGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTatcatc                                                                          <  1:638818/150‑1 (MQ=255)

                                                                              gCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATcc                                                                         >  1:1761893/1‑150 (MQ=255)

                                                                                   gCTTCGGAGGCACCGGGAAAACCTAGTCTACGGCTGATATTGATACCGCTACTACCGCCATATCAATTTAGAGATGGTGCATCCGGGAGGAGTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGGTGCTAAAGCAACGTTATCATCCCTCGt                                                                    <  2:5983/150‑1 (MQ=17)

                                                                                    cTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTg                                                                   >  1:483601/1‑150 (MQ=255)

                                                                                     tACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGC‑TCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGcc                                                                 >  1:1049206/1‑150 (MQ=255)

                                                                                          aTGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCg                                                             >  1:298119/1‑150 (MQ=255)

                                                                                           tGCATCGGTAATTCTTATTCTACTGATGATATTGATACATCTTCTATAGCCATATCATTTATGAGATTGTGCATCCGGGTGTATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCgg                                                            <  2:891147/150‑1 (MQ=17)

                                                                                               tCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTaa                                                        <  2:1248125/150‑1 (MQ=255)

                                                                                                 gggAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAAtt                                                      >  1:1573227/1‑150 (MQ=255)

                                                                                                 gggAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATAACAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGCTTAAtt                                                      >  2:2675736/1‑150 (MQ=255)

                                                                                                  ggAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTc                                                     >  1:278743/1‑150 (MQ=255)

                                                                                                    aaaaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCATAGAGGCTCGGCGTTCG‑GGCCGTTGATAAAGCAACGGTATCATCCCTAGTGACAGCGGATAAtagat                                                   >  2:2913510/1‑146 (MQ=18)

                                                                                                     aaaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGACATACTAGCCTAGCCGCTCGCCCTACG‑GGCCTATGCTAAAGCGAAGATATCATCCCTAGACCCTGCGGTAAATTCCGc                                                  >  2:2452427/1‑150 (MQ=18)

                                                                                                     aaaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGA                                                                                                                                   >  2:2365852‑M1/1‑70 (MQ=255)

                                                                                                     aaaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGA                                                                                                                                   <  1:2365852‑M1/70‑1 (MQ=255)

                                                                                                         ttATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggat                                               >  1:548139‑M1/1‑71 (MQ=255)

                                                                                                         ttATTCTACTGCTGATATTGATACCGCTACTTAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAcc                                              <  1:2773320/150‑1 (MQ=255)

                                                                                                           aTTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattc                                             <  1:125275‑M1/150‑82 (MQ=255)

                                                                                                               tACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCg                                        >  1:1184862/1‑150 (MQ=255)

                                                                                                                 tctctgataTTTATACCGCTTCTAAAGGCATATGAAGTATTAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgtaccc                                       <  2:2808780‑M1/147‑88 (MQ=255)

                                                                                                                    ctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTccaac                                                                                                                         >  1:813687‑M1/1‑60 (MQ=255)

                                                                                                                    ctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATAcc                                   >  2:604664/1‑150 (MQ=255)

                                                                                                                      gATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCtt                                 <  1:730516/150‑1 (MQ=255)

                                                                                                                      gATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCtt                                 >  1:2647334/1‑150 (MQ=255)

                                                                                                                      gATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCtt                                 >  1:2003401/1‑150 (MQ=255)

                                                                                                                      gATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCATGGCGGCTCGCCCTGCG‑GGCCGTTGCTAAAGCAACGTTGACATCCCTAGTGCCTGCGGTTAATTCCGCTAGCACTTCGATACCtt                                 >  1:2647307/1‑150 (MQ=17)

                                                                                                                       atatTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTc                                >  1:1321802/1‑150 (MQ=255)

                                                                                                                       atatTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTc                                <  1:2215051/150‑1 (MQ=255)

                                                                                                                          ttGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCATGCGGCTCGCCATACT‑GGCCGATGCTAAAGAAACGATATCATCCCTAGTGCCATCGGTTAATTCCGCTACATCGACGATACCTTCAtt                             >  1:1868375/1‑150 (MQ=17)

                                                                                                                            gATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGc                           >  2:363881/1‑150 (MQ=255)

                                                                                                                              tACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCAGCTACCTCTTCGATACCTTCATTGCTg                         >  2:1901878/1‑150 (MQ=255)

                                                                                                                               aCCGCTTCTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGa                        <  2:1312876/150‑1 (MQ=255)

                                                                                                                                 cGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaaccccggatgcagcttttga                       <  1:169852‑M1/150‑104 (MQ=255)

                                                                                                                                  gCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGa                     >  1:2221449/1‑150 (MQ=255)

                                                                                                                                  gCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTACG‑GGCCGATGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGa                     >  2:2734906/1‑150 (MQ=255)

                                                                                                                                   ctactaAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaaccccggatgcagcttttgacc                     <  2:548139‑M1/150‑106 (MQ=255)

                                                                                                                                      ctaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATACCGCTAACTCTTCGATACCTTCATTGCTGAAGATTTc                 >  1:1502480/1‑150 (MQ=255)

                                                                                                                                      ctaAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaaccccggatgcagcttttgaccgca                  >  2:115929‑M1/1‑42 (MQ=255)

                                                                                                                                        aaaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCgg               <  1:618394/150‑1 (MQ=255)

                                                                                                                                         aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga              >  1:1331280/1‑150 (MQ=255)

                                                                                                                                         aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga              <  2:1144122/150‑1 (MQ=255)

                                                                                                                                         aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTGATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga              <  1:430442/150‑1 (MQ=255)

                                                                                                                                         aaGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaaccccggatgcagcttatgaccgcatac               >  2:209073‑M1/1‑39 (MQ=255)

                                                                                                                                          aGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGgag             >  1:1544328/1‑150 (MQ=255)

                                                                                                                                           gCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGgaga            >  1:461231/1‑150 (MQ=255)

                                                                                                                                           gCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGgaga            >  2:1892056/1‑150 (MQ=255)

                                                                                                                                               tatCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaaccccggatgcagcttttgaccgcatactccctt         <  1:115929‑M1/150‑118 (MQ=255)

                                                                                                                                                 tCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATgg      >  1:2443758/1‑150 (MQ=255)

                                                                                                                                                  cAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGt     >  2:807741/1‑150 (MQ=255)

                                                                                                                                                   aaTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTg                                                                                                                    >  1:1991383/1‑39 (MQ=40)

                                                                                                                                                   aaTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTg    <  1:1704439/150‑1 (MQ=255)

                                                                                                                                                     ttAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTc                                                                                                       <  1:189004/50‑1 (MQ=32)

                                                                                                                                                     ttAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTc                                                                                                       >  2:188965/1‑50 (MQ=32)

GAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCG‑GGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCA  >  NC_000913/2817767‑2818065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`