Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,046,050 T→G 10.0% intergenic (‑149/+118) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,046,0500TG10.0% 302.1 / 4.2 134intergenic (‑149/+118)ygfF/gcvPputative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base T (37/80);  new base G (11/6);  total (48/86)
Fisher's exact test for biased strand distribution p-value = 1.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

TTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGC  >  NC_000913/3045901‑3046199
                                                                                                                                                     |                                                                                                                                                     
tttGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGt                                                                                                                                                       >  1:1216137/1‑150 (MQ=255)
 ttGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGtt                                                                                                                                                      >  1:2492707/1‑150 (MQ=255)
   gTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTcc                                                                                                                                                    <  2:2066418/150‑1 (MQ=255)
   gTTTTTCCGAGAAAAATTAAGTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTcc                                                                                                                                                    <  2:2066391/150‑1 (MQ=255)
    tttttCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTccc                                                                                                                                                   >  2:569465/1‑150 (MQ=255)
       ttCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTccccgc                                                                                                                                                >  2:2037888/1‑148 (MQ=255)
       ttCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTccccac                                                                                                                                                >  2:2037929/1‑148 (MQ=255)
       ttCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTc                                                                                                                                                >  1:924745/1‑150 (MQ=255)
          cGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCAcc                                                                                                                                             >  1:1445236/1‑150 (MQ=255)
           gagaAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCAccc                                                                                                                                            >  2:1035358/1‑150 (MQ=255)
           gagaAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCGCAccc                                                                                                                                            >  1:1977940/1‑150 (MQ=255)
           gagaAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCACAccc                                                                                                                                            >  2:276886/1‑150 (MQ=255)
            agaAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCACAccca                                                                                                                                           >  1:1233055/1‑149 (MQ=255)
               aaaaTTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAAc                                                                                                                                        <  2:1734008/150‑1 (MQ=255)
               aaaaTTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATATTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAAc                                                                                                                                        <  2:1734031/150‑1 (MQ=255)
                 aattaattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAAccc                                                                                                                                      <  1:538048/150‑1 (MQ=255)
                  attaattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCct                                                                                                                                     <  2:438670/150‑1 (MQ=255)
                  attaattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCct                                                                                                                                     <  1:1295009/150‑1 (MQ=255)
                  attaattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCct                                                                                                                                     <  1:1294875/150‑1 (MQ=255)
                   ttaattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCctc                                                                                                                                    <  1:2900320/150‑1 (MQ=255)
                    taattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACCCCCAAAcccaca                                                                                                                                   >  1:2700347/1‑147 (MQ=255)
                     aattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCtctc                                                                                                                                  <  1:1498416/150‑1 (MQ=255)
                     aattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCtctc                                                                                                                                  <  1:2907960/150‑1 (MQ=255)
                     aattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCtctc                                                                                                                                  <  1:2459584/150‑1 (MQ=255)
                            tGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAg                                                                                                                           <  1:2000967/150‑1 (MQ=255)
                             gACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAgg                                                                                                                          <  2:1647727/150‑1 (MQ=255)
                                   tCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTGGGCACGCTTTTCCCCCTCGCCCTTTCCGGGTGAGGGCCGGGGTGAGGGTAAATCTTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAg                                                                                                                    <  1:617672/150‑1 (MQ=255)
                                    cTTTGAGATTGCTAACAACCGGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAgg                                                                                                                   <  2:825320/150‑1 (MQ=37)
                                         agaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACt                                                                                                              >  2:931591/1‑150 (MQ=255)
                                         agaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGAggggccg                                                                                                              >  2:931593/1‑147 (MQ=255)
                                             tGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCt                                                                                                          <  1:2114119/150‑1 (MQ=255)
                                             tGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCt                                                                                                          <  1:1357467/150‑1 (MQ=255)
                                             tGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCt                                                                                                          <  1:1706731/150‑1 (MQ=255)
                                              gCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTg                                                                                                         <  2:878950/150‑1 (MQ=255)
                                                  aCAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCa                                                                                                     >  2:234118/1‑150 (MQ=255)
                                                    aaaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCAAACCCTCTACCCAAAGGGGCGAGGGGCCTGTCTGAGcccg                                                                                                   >  1:2877810/1‑147 (MQ=255)
                                                     aaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCAAACCCACACCCCAAAGGGGCGAGGGGACTGTCTGAGCACtt                                                                                                  >  1:2012122/1‑150 (MQ=255)
                                                        tGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCGCACCCGAACCCACACCCCCAAGGGGCGGGGGGGCTGTCTGAGCACGTTTg                                                                                               >  2:597869/1‑150 (MQ=255)
                                                        tGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGCGGGGACTGTCTGAGCACTTTTg                                                                                               >  2:2640325/1‑150 (MQ=255)
                                                            tCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCAAACCCTCTCCCCCCAGGGGCGAGGGGCCGGGCTGATCACTGTTGTAcg                                                                                           >  2:2872014/1‑149 (MQ=255)
                                                                 cTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTc                                                                                      <  1:2172220/150‑1 (MQ=255)
                                                                  ttCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCACACCCAAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCGGAGCCCTTTTGTACGTTGTCa                                                                                     >  2:2794531/1‑150 (MQ=25)
                                                                    cAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAACCCACTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATc                                                                                   >  2:1361991/1‑150 (MQ=255)
                                                                     aaTGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCACACCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCt                                                                                  >  2:2335165/1‑150 (MQ=255)
                                                                       tGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGa                                                                                <  2:1416309/150‑1 (MQ=255)
                                                                       tGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGa                                                                                <  1:2087748/150‑1 (MQ=255)
                                                                        gTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGAc                                                                               <  2:1148382/150‑1 (MQ=255)
                                                                         ttGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGCCTGTCTGAGCACTGTTGTACTTTGGCATCTGACt                                                                              >  2:1656577/1‑150 (MQ=255)
                                                                            gCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTaaa                                                                           >  2:1667497/1‑150 (MQ=255)
                                                                            gCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAACCCACTCCCCCAAGGGGCGAGGGGACTGTCTGGGCACGTTTGTACGTTTTCATCTGACTaaa                                                                           >  2:1753682/1‑150 (MQ=255)
                                                                              aCGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTaaaaa                                                                         <  1:559469/150‑1 (MQ=255)
                                                                              aCGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGGGAGGGTAAATATTCGCGCCCGTGCCGGCCTGTTCCCCTCACCCTAACCCGCTCCCCCCAGGGGCGAGGGGACTGTGTGAGCACTTTTGTGCGTTGTCCTCTGACTaaaaa                                                                         >  2:2603060/1‑150 (MQ=25)
                                                                               cGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAg                                                                        <  1:667297/150‑1 (MQ=255)
                                                                                gCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCGCACCCAAAACCAAAACCCACAGGGGCGGGGGGGCGGTCTGAGCACGTTTGTACGTTGTCATATGAAGaaaaatg                                                                       >  2:773213/1‑148 (MQ=255)
                                                                                gCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACAACCCAAACCAAAACCCCAACGGGCGAGGGGACAGTCAGGGCACGGTTTTACATTTTCAACTGACaaaaaaag                                                                       >  2:773182/1‑148 (MQ=255)
                                                                                gCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCGCACCCAAACCCTCTCCCCAAAGGGGCGCGGGGACTGTCTGAGCACATTTGTCCGTTGTCATCTGAATAAAAAgg                                                                       >  2:2063216/1‑150 (MQ=25)
                                                                                gCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCACACCCCAAACCACGCCCCAAACGGGCGAGGGGACAGTCTGAGCCCATTTGTACTTTTTCAAATGACTAAAAAgg                                                                       >  2:773183/1‑150 (MQ=255)
                                                                                  ttttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACCCCCAAAACCGCACCCCAAAGGGGCGAGGGGACAGTCTGATCACGTTTTTACGTTGTAATATGACAAAAAAGgcg                                                                     >  2:834324/1‑150 (MQ=21)
                                                                                   tttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGgcgc                                                                    <  2:1216885/150‑1 (MQ=255)
                                                                                   tttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCGCACCCGAACCCGCTCCCCAAAGGGGCGGGGGGGCTGTCTGAGCACGTTTTTACTTTGTCAAAGGACAAAAAAGgcgc                                                                    >  2:872031/1‑150 (MQ=255)
                                                                                         ccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAgc                                                              <  2:2427353/150‑1 (MQ=255)
                                                                                               ccTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCttt                                                        <  2:2110615/150‑1 (MQ=255)
                                                                                               ccTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCttt                                                        <  1:2396020/150‑1 (MQ=255)
                                                                                               ccTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCttt                                                        <  1:2325779/150‑1 (MQ=255)
                                                                                                cTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCCCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTa                                                       <  2:168575/150‑1 (MQ=255)
                                                                                                cTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTa                                                       <  1:1713667/150‑1 (MQ=255)
                                                                                                 tttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAg                                                      <  2:1445236/150‑1 (MQ=255)
                                                                                                 tttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTACCCGCACCCAAACCCCCACCCCCAAGGGGCGCGGGGGCGGTCTGAGGACATTTGTTCTTTTTAAAAAGACAAAAAAAGCGCCCAAGCGccgggag                                                      >  1:1819915/1‑145 (MQ=255)
                                                                                                  ttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCttttta                                                     <  1:1379430/150‑4 (MQ=25)
                                                                                                  ttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTGCCCACACCCATAACCACACCCCCAATGGGCGTGGGGTCTGGGGGGGCACGTTTTGACGTTTTCCTATGAATAAAATTGAGCCGAAGGGccgggtta                                                     >  1:1934746/1‑144 (MQ=255)
                                                                                                   tCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaa                                                    <  1:938897/150‑1 (MQ=255)
                                                                                                     aGGGAGCGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaaaa                                                  <  1:2564492/150‑1 (MQ=255)
                                                                                                     aGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaaaa                                                  <  1:1035358/150‑1 (MQ=255)
                                                                                                     aGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaaaa                                                  <  2:1287002/150‑1 (MQ=255)
                                                                                                       ggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATa                                                <  2:2154849/150‑1 (MQ=255)
                                                                                                       ggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATa                                                <  2:2154736/150‑1 (MQ=255)
                                                                                                       ggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATa                                                <  1:234118/150‑1 (MQ=255)
                                                                                                       ggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCTCACCCAAACCCACACCCCAAAGGGGCGAGGGGACAGTCTGAGCACTTTTTTAAATTGTCATAAGAAAAAAAAAGCGCCCAAGCGCCTATAGaaaaaa                                                >  2:25117/1‑148 (MQ=21)
                                                                                                             ggCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGaa                                          <  2:1533140/150‑1 (MQ=255)
                                                                                                              gCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAAt                                         <  2:1398070/150‑1 (MQ=255)
                                                                                                              gCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAAt                                         <  2:1398057/150‑1 (MQ=255)
                                                                                                               ccGGGGTGAGGGTAAATATTCGCGCCACTGCCGGCCTGTTCCCCACACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATc                                        <  1:849182/150‑1 (MQ=255)
                                                                                                                 ggggTGAGGGTACATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAg                                      <  2:390917/150‑1 (MQ=255)
                                                                                                                 ggggTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAg                                      <  2:1963543/150‑1 (MQ=255)
                                                                                                                        gggTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTa                               <  1:797309/150‑1 (MQ=255)
                                                                                                                          gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                             <  2:2307538/150‑1 (MQ=255)
                                                                                                                          gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                             <  2:887288/150‑1 (MQ=255)
                                                                                                                          gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                             <  1:1276756/150‑1 (MQ=255)
                                                                                                                          gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                             <  1:2462175/150‑1 (MQ=255)
                                                                                                                           tAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTg                            <  1:1667497/150‑1 (MQ=255)
                                                                                                                           tAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTg                            <  2:1039550/150‑1 (MQ=255)
                                                                                                                           tAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTg                            <  1:1509985/150‑1 (MQ=255)
                                                                                                                             aaTATTCGCGCCAGTGCCGGCCTGGTCCCCACACCCAAAACCACACCCCCAAGGGGCGGGGGGGCAGTCTGAGCACGTTTGTACGTTTTAATCTGACTAAAAAAGCGCCGCAGCGCCGTTAGAAAATAGTAGAATAAGTGAATTAATGGt                          >  2:1244756/1‑150 (MQ=255)
                                                                                                                                  tCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCg                     <  1:1836255/150‑1 (MQ=255)
                                                                                                                                  tCGCGCCAGTGCCGGCCTGTGCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCg                     <  2:2529210/150‑1 (MQ=255)
                                                                                                                                    gcgcCAGTGCCGGCCTGTTCCCCACACCCAAAACCACTCCCCAAATGGGCGCGGGGACAGTGAGAGCACATTTGTACGTTGTCAAAAGAATAAAAAGGCGCCGAAGCGCCGTTAGAAAAAAGTCGAAACAGTTAAATACTGGTATTAGCt                   >  2:585258/1‑150 (MQ=255)
                                                                                                                                                 ccTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAg      <  1:884120/150‑1 (MQ=255)
                                                                                                                                                 ccTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAg      <  1:883936/150‑1 (MQ=255)
                                                                                                                                                 ccTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGACGGGACTGTCTGAGCACTTTTGTACTTTGACATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAg      <  1:2219006/150‑1 (MQ=255)
                                                                                                                                                     ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGGACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGc  >  2:1307400/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGC  >  NC_000913/3045901‑3046199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: