Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,046,136 A→C 5.2% intergenic (‑235/+32) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,046,1360AC 5.2% 471.8 / 11.7 138intergenic (‑235/+32)ygfF/gcvPputative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base A (49/80);  new base C (2/6);  total (52/86)
Fisher's exact test for biased strand distribution p-value = 7.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

CCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGC  >  NC_000913/3045989‑3046284
                                                                                                                                                   |                                                                                                                                                    
cccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAg                                                                                                                                                    <  1:2152077/150‑1 (MQ=255)
 ccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAgc                                                                                                                                                   <  2:2427353/150‑1 (MQ=255)
   tCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAgcgc                                                                                                                                                 <  2:69251/150‑1 (MQ=255)
       ccTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCttt                                                                                                                                             <  1:2428598/150‑1 (MQ=255)
       ccTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCttt                                                                                                                                             <  1:2396020/150‑1 (MQ=255)
        cTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTa                                                                                                                                            <  1:1713667/150‑1 (MQ=255)
        cTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATAGTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCtttt                                                                                                                                            <  1:1881634/150‑2 (MQ=37)
         tttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAg                                                                                                                                           <  2:1384724/150‑1 (MQ=255)
         tttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAg                                                                                                                                           <  2:1445236/150‑1 (MQ=255)
         tttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAg                                                                                                                                           <  2:1445171/150‑1 (MQ=255)
         tttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTACCCGCACCCAAACCCCCACCCCCAAGGGGCGCGGGGGCGGTCTGAGGACATTTGTTCTTTTTAAAAAGACAAAAAAAGCGCCCAAGCGccgggag                                                                                                                                           >  1:1819915/1‑145 (MQ=255)
          ttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCttttta                                                                                                                                          <  1:1379430/150‑4 (MQ=25)
          ttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTGCCCACACCCATAACCACACCCCCAATGGGCGTGGGGTCTGGGGGGGCACGTTTTGACGTTTTCCTATGAATAAAATTGAGCCGAAGGGccgggtta                                                                                                                                          >  1:1934746/1‑144 (MQ=255)
            cAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaaa                                                                                                                                        <  2:128539/150‑1 (MQ=255)
             aGGGAGCGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaaaa                                                                                                                                       <  1:2564492/150‑1 (MQ=255)
             aGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaaaa                                                                                                                                       <  1:1035358/150‑1 (MQ=255)
               ggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATa                                                                                                                                     <  2:2154736/150‑1 (MQ=255)
               ggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATa                                                                                                                                     <  2:2154849/150‑1 (MQ=255)
               ggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATa                                                                                                                                     <  1:234118/150‑1 (MQ=255)
               ggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCTCACCCAAACCCACACCCCAAAGGGGCGAGGGGACAGTCTGAGCACTTTTTTAAATTGTCATAAGAAAAAAAAAGCGCCCAAGCGCCTATAGaaaaaa                                                                                                                                     >  2:25117/1‑148 (MQ=21)
                     ggCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGaa                                                                                                                               <  2:1533140/150‑1 (MQ=255)
                      gCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAAt                                                                                                                              <  2:1398070/150‑1 (MQ=255)
                       ccGGGGTGAGGGTAAATATTCGCGCCACTGCCGGCCTGTTCCCCACACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATc                                                                                                                             <  1:849182/150‑1 (MQ=255)
                         ggggTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAg                                                                                                                           <  2:1963543/150‑1 (MQ=255)
                         ggggTGAGGGTAAATATTCGCGCCAGTGCCGGCCCGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAg                                                                                                                           <  2:2522504/150‑1 (MQ=255)
                                gggTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTa                                                                                                                    <  1:797309/150‑1 (MQ=255)
                                  gTAAATATTCGCGCCAGTGCCGTCCTGTTCCCCTAACCCTAACCCTCTCCCAAAAGGGGCGAGGGGAATGTCTGAGCTATTTTGTACTTTTTCATCTGTCTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                                                                                                                  <  2:851306/150‑1 (MQ=255)
                                  gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                                                                                                                  <  2:2307538/150‑1 (MQ=255)
                                  gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                                                                                                                  <  1:910946/150‑1 (MQ=255)
                                  gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                                                                                                                  <  2:887288/150‑1 (MQ=255)
                                  gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCACAACCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                                                                                                                  >  2:2307389/1‑150 (MQ=255)
                                  gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCTAACCCACTCCCCAAAGGGGCGAGGGGCCGGTATGAGCACATTTGTACATTGTCAAAATACAAAAAAGGCGCCGCAGCGCCTTTATAAAATAGTCGAATCAGTGAATTAca                                                                                                                  >  2:1735582/1‑149 (MQ=255)
                                   tAACTATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTg                                                                                                                 <  1:1753682/150‑1 (MQ=255)
                                   tAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTTACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCTAATCAGTGAATTACTg                                                                                                                 <  1:81983/150‑1 (MQ=255)
                                   tAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTg                                                                                                                 <  1:1667497/150‑1 (MQ=255)
                                   tAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTg                                                                                                                 <  2:1039550/150‑1 (MQ=255)
                                     aaTATTCGCGCCAGTGCCGGCCTGGTCCCCACACCCAAAACCACACCCCCAAGGGGCGGGGGGGCAGTCTGAGCACGTTTGTACGTTTTAATCTGACTAAAAAAGCGCCGCAGCGCCGTTAGAAAATAGTAGAATAAGTGAATTAATGGt                                                                                                               >  2:1244756/1‑150 (MQ=255)
                                          tCGCGCCAGTGCCGGCCTGTGCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCg                                                                                                          <  2:2529210/150‑1 (MQ=255)
                                            gcgcCAGTGCCGGCCTGTTCCCCACACCCAAAACCACTCCCCAAATGGGCGCGGGGACAGTGAGAGCACATTTGTACGTTGTCAAAAGAATAAAAAGGCGCCGAAGCGCCGTTAGAAAAAAGTCGAAACAGTTAAATACTGGTATTAGCt                                                                                                        >  2:585258/1‑150 (MQ=255)
                                                         ccTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAg                                                                                           <  1:884120/150‑1 (MQ=255)
                                                           tggtCCCCTCACCCTCACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGCGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCATTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGa                                                                                         <  2:2374706/147‑1 (MQ=255)
                                                             ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGGACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGc                                                                                       >  2:1307400/1‑150 (MQ=255)
                                                                cccTCACCCTAACCCTCTCCCCAATGGGGCGAGGTGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGa                                                                                    <  1:773182/150‑1 (MQ=255)
                                                                cccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGa                                                                                    <  2:2290759/150‑1 (MQ=255)
                                                                cccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGa                                                                                    <  1:773183/150‑1 (MQ=255)
                                                                cccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGa                                                                                    <  2:809528/150‑1 (MQ=255)
                                                                cccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGa                                                                                    <  2:2837549/150‑1 (MQ=255)
                                                                cccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGa                                                                                    <  2:1318872/150‑1 (MQ=255)
                                                                 ccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGaa                                                                                   >  1:1736446/1‑150 (MQ=255)
                                                                 ccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGaa                                                                                   <  1:538897/150‑1 (MQ=255)
                                                                 ccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGaa                                                                                   >  1:1018332/1‑150 (MQ=255)
                                                                      cccTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGt                                                                              >  1:1206483/1‑150 (MQ=255)
                                                                       ccTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGtt                                                                             >  1:2835460/1‑150 (MQ=255)
                                                                       ccTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGtt                                                                             >  2:1087038/1‑150 (MQ=255)
                                                                       ccTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGtt                                                                             >  1:2300930/1‑150 (MQ=255)
                                                                        cTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTa                                                                            >  2:302320/1‑150 (MQ=255)
                                                                        cTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTa                                                                            >  2:302301/1‑150 (MQ=255)
                                                                          aaCCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGTACAGGTTACg                                                                          >  2:2069718/1‑150 (MQ=255)
                                                                          aaCCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACg                                                                          <  1:1361991/150‑1 (MQ=255)
                                                                            cccTCGCCCCTTTGGGGAGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGt                                                                        >  1:2400699/1‑150 (MQ=255)
                                                                              ctctCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCg                                                                      <  1:2342362/150‑1 (MQ=255)
                                                                               tctcCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCTAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGc                                                                     <  1:2640527/150‑1 (MQ=255)
                                                                               tctcCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGc                                                                     <  1:597869/150‑1 (MQ=255)
                                                                                   cccAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTa                                                                 >  2:1647237/1‑150 (MQ=255)
                                                                                    ccAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTaa                                                                <  2:866673/150‑1 (MQ=255)
                                                                                    ccAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTaa                                                                >  1:2293120/1‑150 (MQ=255)
                                                                                      aaaGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAAc                                                              >  2:687531/1‑150 (MQ=255)
                                                                                         ggggCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAAcatc                                                           >  2:1851101/1‑150 (MQ=255)
                                                                                         ggggCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAAcatc                                                           >  2:1851091/1‑150 (MQ=255)
                                                                                                 gggACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGAc                                                   >  1:1602505/1‑150 (MQ=255)
                                                                                                        tCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCAc                                            >  2:2727916/1‑150 (MQ=255)
                                                                                                        tCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCAc                                            <  2:2063735/150‑1 (MQ=255)
                                                                                                           gAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGt                                         <  1:2189523/150‑1 (MQ=255)
                                                                                                              cACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCgg                                      <  1:1051819/150‑1 (MQ=255)
                                                                                                                 ttttGTACTTTGTCATCTTACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGAGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCa                                   >  2:2226173/1‑150 (MQ=255)
                                                                                                                 ttttGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCa                                   >  2:65646/1‑150 (MQ=255)
                                                                                                                 ttttGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCa                                   <  1:1244756/150‑1 (MQ=255)
                                                                                                                 ttttGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCa                                   >  2:418554/1‑150 (MQ=255)
                                                                                                                 ttttGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCa                                   >  1:59380/1‑150 (MQ=255)
                                                                                                                 ttttGTACTTAGACATCTGACTAAATAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTATTCGGTAAGCAGGAGCAGAACAGGTTACGGTCGCCGTAATCGTCATCCAGACGTTTCACTGTCGGccc                                   >  2:2226169/1‑149 (MQ=255)
                                                                                                                    tGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTa                                <  2:1018332/150‑1 (MQ=255)
                                                                                                                    tGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTa                                <  2:1736446/150‑1 (MQ=255)
                                                                                                                    tGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTa                                <  1:1185625/150‑1 (MQ=255)
                                                                                                                      tACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTCtt                              >  2:756319/1‑150 (MQ=255)
                                                                                                                           tGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCt                         <  2:913483/150‑1 (MQ=255)
                                                                                                                           tGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCt                         <  2:1080501/150‑1 (MQ=255)
                                                                                                                             tCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTTGTATTCGCTAATCTGTACGCAGGATCTGATCAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGc                       <  1:900842/150‑1 (MQ=255)
                                                                                                                               aTCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCca                     <  2:1216137/150‑1 (MQ=255)
                                                                                                                                    aCTAAAAAGGCGCCGAGCGCCTTTAGAAAATAGTCGAAACAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCCGTATTTGTCTGCCACACCTg               >  1:2612199/1‑150 (MQ=255)
                                                                                                                                      tAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGc              >  2:579034/1‑150 (MQ=255)
                                                                                                                                        aaaaGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCg            >  2:1990025/1‑150 (MQ=255)
                                                                                                                                        aaaaGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATAACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCg            >  2:1894132/1‑150 (MQ=255)
                                                                                                                                         aaaGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCgg           <  2:924745/150‑1 (MQ=255)
                                                                                                                                         aaaGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCgg           <  1:2037929/150‑1 (MQ=255)
                                                                                                                                             gcgcCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATATGTCTGCCACACCTGCAGGGAAt       >  2:2187670/1‑150 (MQ=255)
                                                                                                                                              cgcCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGATACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATa      >  2:1707326/1‑150 (MQ=255)
                                                                                                                                                ccGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATAcc    <  1:2307389/150‑1 (MQ=255)
                                                                                                                                                ccGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATAcc    <  2:1977940/150‑1 (MQ=255)
                                                                                                                                                ccGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATAcc    <  1:276886/150‑1 (MQ=255)
                                                                                                                                                 cGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCg   <  2:2794440/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGC  >  NC_000913/3045989‑3046284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: