Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,270,082 A→C 5.3% intergenic (+480/+134) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,0820AC 5.3% 351.2 / 3.4 136intergenic (+480/+134)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base A (74/48);  new base C (4/5);  total (80/56)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

ATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAC  >  NC_000913/3269935‑3270228
                                                                                                                                                   |                                                                                                                                                  
   atacacccccTCCAAAACAAAAAAACATCCGAAGCGGGTAATTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                                                                               <  2:863720/144‑1 (MQ=11)
    ttCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCCTTCATCTTTCTGCCCCGACAAAAGCAAAAACCCGCCGAAGCgcg                                                                                                                                              >  1:1516847/1‑148 (MQ=255)
    ttCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCggg                                                                                                                                              <  1:2210700/150‑1 (MQ=37)
      cTGCCCCTCCAAAAGTAAAAACCCGCCGCAGCGGGTTTTTACGTACAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtt                                                                                                                                            <  2:2022139/150‑1 (MQ=255)
         ccccTCCACAAGTAACAACCCGCCGACGCGGGCTTTTACGTCAACCAGGTGACACTGACCGCTCCGCCGCTGTCTTTTGGGTATCGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGttttt                                                                                                                                         <  2:163691/150‑1 (MQ=11)
         ccccTCCAAAAGTAAAACCCCGCCGAATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGttttt                                                                                                                                         <  2:1510448/150‑1 (MQ=11)
          cccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                                                                        <  1:1141615/150‑1 (MQ=37)
          cccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                                                                        >  2:2228740/1‑150 (MQ=37)
          ccatCCTAAATTATAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                                                                        <  2:365087/147‑1 (MQ=11)
              ccAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCGTTTATGTTTCTGGGGGTGGAAAAGCATTAACCCGCCGTAGCGGGTGTTTAAGTa                                                                                                                                    >  2:652454/1‑150 (MQ=11)
              ccAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTGTCGGGTAGAGTGGCGAGGACCGTCGTTCATCGATCTGCCCAACCCAAACCAAAAACACGCCGAGGCCGGGGTTTAAGTa                                                                                                                                    >  2:1540059/1‑150 (MQ=2)
               tttacgTAAAAACCCGCTTCGGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaa                                                                                                                                   >  1:2121862/6‑150 (MQ=11)
               cacaaGTAAAAACCCGCCGAAGCGGGTTATTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaa                                                                                                                                   <  2:185387/147‑1 (MQ=255)
                 aaaGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAc                                                                                                                                 <  2:1955859/150‑1 (MQ=38)
                     tAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGGAAAAAACCGCCGAAGCGGGTTTTTTCTGAATCCAgg                                                                                                                             >  2:549929/1‑150 (MQ=11)
                           cccGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGGGTTTTTCGTAAAACAGGTGCAAc                                                                                                                       >  2:2298026/1‑150 (MQ=11)
                            ccgccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTGTGGGGATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTGTTACGTAAACCAGGTGAAACt                                                                                                                      >  1:2804547/1‑150 (MQ=255)
                              gccgAAGCGGGTTTTTGCGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGa                                                                                                                    >  2:1707592/1‑150 (MQ=12)
                              gccgAACCGGTATATTACGTAAAACAGGAGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa                                                                                                                    <  1:643628/150‑1 (MQ=11)
                               ccgAAGCGGGTTTTTACGTCAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAc                                                                                                                   <  2:1044385/150‑1 (MQ=37)
                               ccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAc                                                                                                                   >  2:1744783/1‑150 (MQ=17)
                               ccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAc                                                                                                                   <  2:834976/150‑1 (MQ=37)
                                cgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAcc                                                                                                                  <  1:1615410/150‑1 (MQ=37)
                                 gAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCg                                                                                                                 >  2:1041182/1‑150 (MQ=37)
                                 gAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAAACAGGTGAAACTGACCg                                                                                                                 >  2:14635/1‑150 (MQ=21)
                                    gCGGGTTTTTACGTACAACAGGTTAAACTGAGCGATAAGCCGCTTTCTTTTGGGTTTAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATa                                                                                                              <  2:1148558/150‑1 (MQ=255)
                                    gCGGGTTTTTACGTAACACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATa                                                                                                              <  2:1960499/150‑1 (MQ=37)
                                    gCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATa                                                                                                              <  2:1148914/150‑1 (MQ=37)
                                     cGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATaa                                                                                                             >  1:1155142/1‑150 (MQ=37)
                                     cGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATaa                                                                                                             >  2:2824044/1‑150 (MQ=37)
                                       ggTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGc                                                                                                           >  1:2349975/1‑150 (MQ=37)
                                       ggTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGc                                                                                                           >  1:1528391/1‑150 (MQ=37)
                                        gTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGcc                                                                                                          <  1:1278427/150‑1 (MQ=37)
                                        gTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGcc                                                                                                          <  1:1278164/150‑1 (MQ=37)
                                           tttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGTAGCGGGTTTTTACGGAAACCAGGGGAAACTGACCGATTAGCCGcg                                                                                                       >  1:2744273/1‑149 (MQ=255)
                                             tACGTAAATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGcttt                                                                                                     <  1:408466/150‑1 (MQ=17)
                                                gTAAAACAGGTGAAACTGACCGATACGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttctt                                                                                                  <  2:2129511/150‑1 (MQ=255)
                                                  aaatCAGGTGAAACTGACCGATTAGCCGCTTTCTTTTTGGTATTGTGTCGTGTACAGTCATTCATATTTCTGCCCCTCCAAAATCAAAAACCCGCCGAAGCGGTTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCtttt                                                                                                <  1:1679576/150‑1 (MQ=11)
                                                  aaaaCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGTGTCGTGGACAGGCATTCATCTTTATGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGTAAAACAGGTGTAAATGACCGATAAGCCGCGTTCtttt                                                                                                >  1:786928/1‑150 (MQ=11)
                                                   aaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttctgtgg                                                                                               >  2:1526587/1‑146 (MQ=255)
                                                   aaaCAGGTGAAACCGACCGTTAAGCCGCTTTCTTTTTGGTTTTGTGTCGTGGACAGTGTTTGATATTTCTGCCCCTCCAAAAGCTTAAACCCGCCGTATCGGGTTTTTACGTACACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                               <  2:574385/150‑1 (MQ=14)
                                                    cacacGTGACTCTGACCGACCCGCCGCCTGCTTTGGGGTCGCGCGTCGTGGACAGCCAGTCATCCTCCTGCCCCTCCCCAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                              <  2:2794676/149‑1 (MQ=2)
                                                    atcAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                              <  2:2794708/148‑1 (MQ=11)
                                                    atcAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                              <  2:1329749/148‑1 (MQ=11)
                                                    aaCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                              <  1:453582/150‑1 (MQ=25)
                                                    aaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                              <  1:2030251/150‑1 (MQ=38)
                                                    aaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTgg                                                                                              >  1:2456822/1‑150 (MQ=38)
                                                     aCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTggg                                                                                             <  1:2059155/150‑1 (MQ=38)
                                                       aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATCAGGCGCTTTCTTGTGGGta                                                                                           >  2:2516143/1‑150 (MQ=255)
                                                          tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTagag                                                                                        >  2:2590860/1‑150 (MQ=255)
                                                            aaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCAACTTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGGGCAACTGACCGATAAGCCGCTTTCGTTTGGGTATAGcg                                                                                      >  1:2796592/1‑150 (MQ=11)
                                                              aCTGACCGATAAGCCGCTTTCGTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTCTAGcggc                                                                                    >  2:2612082/1‑148 (MQ=255)
                                                                tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgt                                                                                  <  1:1035802/150‑1 (MQ=37)
                                                                tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTAAAGagtagt                                                                                  >  2:945958/1‑150 (MQ=255)
                                                                            cGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCAACATTAAGCCCCAACAAAAACAAAAAACCCCCGCACCCGGTTTTTACGTAAAACACGTGAAAAAGAACGAAAAGCCGCGTTATTTTGTGTATAGCGTCGGGGACCGtcagta                                                                      >  1:1054945/1‑147 (MQ=2)
                                                                            cGCTTTCTTTTGGGTATAGTGTCGTGGACAATCAATCAAAATTAATCCCCACCAAAAACAAAAAACCCCCGAAGCCGGTTTTTAACTAAAACACGTGAAAAAGAACCATAAACCGCTTTATTTTTGGTAAAGCCTAGTGGACAGacatta                                                                      >  1:1054942/1‑149 (MQ=2)
                                                                               tttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGAGTCGTGGACAGTCAatcatc                                                                   >  1:176805/1‑150 (MQ=255)
                                                                               tttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcattcata                                                                   >  1:222162/1‑149 (MQ=17)
                                                                                   ttttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAATGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcaatcataatta                                                               >  1:380809/1‑145 (MQ=255)
                                                                                    tttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCAATCAAATTTCt                                                              >  2:5347/1‑150 (MQ=255)
                                                                                        ggTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGccc                                                          <  1:948938/150‑1 (MQ=37)
                                                                                         gTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTAATTAATAtttaatcacc                                                         >  2:2726115/1‑143 (MQ=11)
                                                                                         gTATAGTGTCGTGGACAGTAATTCATCTTTCTGCCCCTCCAAAAGCAAAAACACGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTAGTGGGATAGAGTCGTGTACAGGCCATCATAGTTCAGcccc                                                         >  2:2404302/1‑150 (MQ=11)
                                                                                              gtgtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGCGGCGGGGACAGTCAGTCAAAATTCTGCCCCTccca                                                    >  1:1633935/1‑148 (MQ=11)
                                                                                                gtCGTGGACTGTCATTCTTCTTTCTGGCCCTCCACAATCATAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTGATCTTTCTGCCCCTCCaaaa                                                  <  2:1952950/150‑1 (MQ=11)
                                                                                                gtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                  <  1:2035784/150‑1 (MQ=37)
                                                                                                gtCGTGGAAAGTCACTCATCTTTCTGCCACTCCAAAAGCACAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                  <  1:1274212/150‑1 (MQ=255)
                                                                                                  cGTGGACAGCCAGTCATCTATCTGCCACTCCAAAAGCAAAAACCCGCCGTAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGc                                                <  1:1992137/150‑1 (MQ=11)
                                                                                                   gTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCa                                               <  1:1393871/150‑1 (MQ=37)
                                                                                                    tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaa                                              >  2:275023/1‑150 (MQ=37)
                                                                                                        cAGTCGTGCAACTTTATGCCCCAACAAAAGCAAAAAACCCCCGCAGCGGGTTTTTACGTAAAACAGGTGAAAATGAACCATAAGCCCCGTTATTTTGGGTATAGCGTCGTGGCCAGTAAATCATAATTCAGCCCCCCCaaaaacaaaaac                                          >  2:2057633/1‑150 (MQ=2)
                                                                                                         aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAcc                                         >  1:986512/1‑150 (MQ=37)
                                                                                                          gTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGGCATTCATATTTCTGCCCCGCCAAAATCAATAAccc                                        >  2:2585424/1‑150 (MQ=11)
                                                                                                              ttcaaCTCTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGGTTATTACGTACCCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCTAAAACccgccg                                    <  2:2883405/150‑1 (MQ=11)
                                                                                                                 atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAg                                 >  1:545008/1‑150 (MQ=37)
                                                                                                                  tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCAATCAAGATTGATCCCCAACAAAACGCAAAACCCGCCGCAgg                                >  2:2224141/1‑149 (MQ=11)
                                                                                                                     ttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCGTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCggg                             >  2:856308/1‑150 (MQ=37)
                                                                                                                         gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcatt                                                                       <  2:921272/104‑1 (MQ=40)
                                                                                                                         gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcatt                                                                       >  1:2425955/1‑104 (MQ=40)
                                                                                                                         gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcatt                                                                       <  2:2425955/104‑1 (MQ=40)
                                                                                                                         gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcatt                                                                       >  1:921272/1‑104 (MQ=40)
                                                                                                                         gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGtttt                         >  2:133770/1‑150 (MQ=255)
                                                                                                                         gCCCCACCAACAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCACAAGCAAAAACCCGCCGAAGCGGGtttt                         <  1:2140644/150‑1 (MQ=255)
                                                                                                                           cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                       >  1:175852/1‑150 (MQ=37)
                                                                                                                            ccTCCAAACGCAAAAACCCGCCGACGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                      <  2:2579079/150‑1 (MQ=255)
                                                                                                                             cTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACg                     >  2:1718960/1‑150 (MQ=37)
                                                                                                                             cTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTaag                     >  2:2877066/1‑148 (MQ=37)
                                                                                                                                 aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGGCCTTCATCTTTCGGCCCCGCCCAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGGaaa                 >  1:755975/1‑150 (MQ=11)
                                                                                                                                  aaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAt                >  2:1014409/1‑150 (MQ=38)
                                                                                                                                  aaaGCAAAAACCAGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCTCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAt                <  2:768325/150‑1 (MQ=255)
                                                                                                                                      taaaaaCCCGCTTCGGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg            >  2:155198/2‑150 (MQ=11)
                                                                                                                                       aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTCTAGCGTCCTGGACACTCATTCATCTTTCAGCCCCTCCAAAAGCAAAAAACCACCGAAGCGGGTATTTTCGAAAATCAggg           >  2:2204380/1‑149 (MQ=11)
                                                                                                                                       aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGGAATAACCAGCCGATGCGGGTTTTTAGGTTAATCAgtt           >  2:1886624/1‑148 (MQ=11)
                                                                                                                                       aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt           >  1:379802/1‑150 (MQ=37)
                                                                                                                                       aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt           >  1:765010/1‑150 (MQ=37)
                                                                                                                                       aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt           >  2:2317697/1‑150 (MQ=37)
                                                                                                                                        aaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGGAAATCAGGTg          >  1:794953/1‑150 (MQ=37)
                                                                                                                                         aaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGa         <  1:2824044/150‑1 (MQ=37)
                                                                                                                                           aCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGaaa       >  1:1178433/1‑150 (MQ=37)
                                                                                                                                             ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAATCAGGTGAAACt     >  2:1113437/1‑150 (MQ=37)
                                                                                                                                                   |                                                                                                                                                  
ATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAC  >  NC_000913/3269935‑3270228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: