Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,270,143 C→T 5.5% intergenic (+541/+73) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,1430CT 5.5% 356.4 / 6.3 128intergenic (+541/+73)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base C (81/36);  new base T (2/5);  total (85/43)
Fisher's exact test for biased strand distribution p-value = 3.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

AAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAA  >  NC_000913/3269995‑3270291
                                                                                                                                                    |                                                                                                                                                    
aaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCAACTTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGGGCAACTGACCGATAAGCCGCTTTCGTTTGGGTATAGcg                                                                                                                                                     >  1:2796592/1‑150 (MQ=11)
  aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtc                                                                                                                                                   >  2:2611935/1‑150 (MQ=37)
    tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgt                                                                                                                                                 <  1:1035802/150‑1 (MQ=37)
    tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTAAAGagtagt                                                                                                                                                 >  2:945958/1‑150 (MQ=255)
                cGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCAACATTAAGCCCCAACAAAAACAAAAAACCCCCGCACCCGGTTTTTACGTAAAACACGTGAAAAAGAACGAAAAGCCGCGTTATTTTGTGTATAGCGTCGGGGACCGtcagta                                                                                                                                     >  1:1054945/1‑147 (MQ=2)
                cGCTTTCTTTTGGGTATAGTGTCGTGGACAATCAATCAAAATTAATCCCCACCAAAAACAAAAAACCCCCGAAGCCGGTTTTTAACTAAAACACGTGAAAAAGAACCATAAACCGCTTTATTTTTGGTAAAGCCTAGTGGACAGacatta                                                                                                                                     >  1:1054942/1‑149 (MQ=2)
                   tttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGAGTCGTGGACAGTCAatcatc                                                                                                                                  >  1:176805/1‑150 (MQ=255)
                   tttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcattcata                                                                                                                                  >  1:222162/1‑149 (MQ=17)
                       ttttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAATGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcaatcataatta                                                                                                                              >  1:380809/1‑145 (MQ=255)
                       ttttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAATCTTTc                                                                                                                              >  1:381260/1‑150 (MQ=255)
                        tttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCAATCAAATTTCt                                                                                                                             >  2:5347/1‑150 (MQ=255)
                           gggTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACACTCAATCAACATTATGcc                                                                                                                          >  2:2773458/1‑150 (MQ=11)
                            ggTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGccc                                                                                                                         <  1:948938/150‑1 (MQ=37)
                             gTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTAATTAATAtttaatcacc                                                                                                                        >  2:2726115/1‑143 (MQ=11)
                             gTATAGTGTCGTGGACAGTAATTCATCTTTCTGCCCCTCCAAAAGCAAAAACACGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTAGTGGGATAGAGTCGTGTACAGGCCATCATAGTTCAGcccc                                                                                                                        >  2:2404302/1‑150 (MQ=11)
                                  gtgtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGCGGCGGGGACAGTCAGTCAAAATTCTGCCCCTccca                                                                                                                   >  1:1633935/1‑148 (MQ=11)
                                    gtCGTGGACTGTCATTCTTCTTTCTGGCCCTCCACAATCATAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTGATCTTTCTGCCCCTCCaaaa                                                                                                                 <  2:1952950/150‑1 (MQ=11)
                                    gtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                                                                                 <  1:2035784/150‑1 (MQ=37)
                                    gtCGTGGAAAGTCACTCATCTTTCTGCCACTCCAAAAGCACAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                                                                                 <  1:1274212/150‑1 (MQ=255)
                                     tCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAg                                                                                                                >  2:11124/1‑150 (MQ=37)
                                      cGTGGACAGCCAGTCATCTATCTGCCACTCCAAAAGCAAAAACCCGCCGTAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGc                                                                                                               <  1:1992137/150‑1 (MQ=11)
                                       gTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCa                                                                                                              <  1:1393871/150‑1 (MQ=37)
                                            cAGTCGTGCAACTTTATGCCCCAACAAAAGCAAAAAACCCCCGCAGCGGGTTTTTACGTAAAACAGGTGAAAATGAACCATAAGCCCCGTTATTTTGGGTATAGCGTCGTGGCCAGTAAATCATAATTCAGCCCCCCCaaaaacaaaaac                                                                                                         >  2:2057633/1‑150 (MQ=2)
                                              gTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAAccc                                                                                                       >  1:2296257/1‑150 (MQ=37)
                                                  ttcaaCTCTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGGTTATTACGTACCCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCTAAAACccgccg                                                                                                   <  2:2883405/150‑1 (MQ=11)
                                                     atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAg                                                                                                >  1:545008/1‑150 (MQ=37)
                                                      tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCAATCAAGATTGATCCCCAACAAAACGCAAAACCCGCCGCAgg                                                                                               >  2:2224141/1‑149 (MQ=11)
                                                             gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcatt                                                                                                                                      >  1:921272/1‑104 (MQ=40)
                                                             gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcatt                                                                                                                                      <  2:921272/104‑1 (MQ=40)
                                                             gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcatt                                                                                                                                      <  2:2425955/104‑1 (MQ=40)
                                                             gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttgt                                                                                        >  1:1140162/1‑148 (MQ=37)
                                                             gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCATTCATCTTTGTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgggggtt                                                                                        >  2:88878/1‑146 (MQ=255)
                                                               cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                      >  1:175852/1‑150 (MQ=37)
                                                                ccTCCAAACGCAAAAACCCGCCGACGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                     <  2:2579079/150‑1 (MQ=255)
                                                                 cTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACg                                                                                    >  2:1718960/1‑150 (MQ=37)
                                                                      aaaGCAAAAACCCGCCGAAGCGTGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTTGTCAGTCATTCATGTTTCTGCCGGTCAAAAATCAAAAAGCCGGCGAAGGGGGTTTTTTCGTTAAt                                                                               >  2:1014389/1‑150 (MQ=11)
                                                                      aaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAt                                                                               >  2:1014409/1‑150 (MQ=38)
                                                                      aaaGCAAAAACCAGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCTCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAt                                                                               <  2:768325/150‑1 (MQ=255)
                                                                          taaaaaCCCGCTTCGGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg                                                                           >  2:155198/2‑150 (MQ=11)
                                                                          ctccaccccGCCGGAGCGGGTTTTGACGTACAGCAGGTGAAACTGACCGATCAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAGTCATCTTTCTGCCCCTCCAAACGCAAACACCCGCCGAAGCGGGTTTTTACGTAAATCAgg                                                                           <  2:286533/144‑1 (MQ=11)
                                                                           aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTCTAGCGTCCTGGACACTCATTCATCTTTCAGCCCCTCCAAAAGCAAAAAACCACCGAAGCGGGTATTTTCGAAAATCAggg                                                                          >  2:2204380/1‑149 (MQ=11)
                                                                           aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGGAATAACCAGCCGATGCGGGTTTTTAGGTTAATCAgtt                                                                          >  2:1886624/1‑148 (MQ=11)
                                                                           aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt                                                                          >  2:2317697/1‑150 (MQ=37)
                                                                           aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt                                                                          >  1:765010/1‑150 (MQ=37)
                                                                           aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt                                                                          >  2:834480/1‑150 (MQ=37)
                                                                           aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt                                                                          >  1:379802/1‑150 (MQ=37)
                                                                           aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt                                                                          >  2:1438838/1‑150 (MQ=37)
                                                                           aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCGTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt                                                                          >  2:2002958/1‑150 (MQ=37)
                                                                            aaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGGTGTTTACGTAAATCAGGTg                                                                         >  1:1650481/1‑150 (MQ=255)
                                                                            aaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGGAAATCAGGTg                                                                         >  1:794953/1‑150 (MQ=37)
                                                                             aaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGa                                                                        <  1:2824044/150‑1 (MQ=37)
                                                                                    ccgAAGCGGGTTTTTACGTAACCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAc                                                                 <  2:305851/150‑1 (MQ=37)
                                                                                          cGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATaa                                                           <  2:1221787/150‑1 (MQ=37)
                                                                                           gggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAg                                                          >  1:483795/1‑150 (MQ=37)
                                                                                           gggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAg                                                          >  2:903788/1‑150 (MQ=37)
                                                                                              tttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCg                                                       >  1:1449374/1‑150 (MQ=37)
                                                                                              tttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCg                                                       >  2:2599208/1‑150 (MQ=37)
                                                                                              tttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCg                                                       >  2:2599096/1‑150 (MQ=37)
                                                                                                    cGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCt                                                 >  1:89912/1‑150 (MQ=255)
                                                                                                    cGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCt                                                 >  1:139636/1‑150 (MQ=255)
                                                                                                    cGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAGTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAATCAGGTGAAACTGACCGATAATCCGGGTTCt                                                 >  1:2647460/1‑150 (MQ=255)
                                                                                                     gTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGACGTGGACCGTCATTCAACTTACTTCCCCTCCAAAAGCAAAAACCCCCCCAACCCGGTTTTTACGTAAATCACGTGAAACAAACCCATAAGCCGGGTTcat                                                >  1:2911045/1‑148 (MQ=14)
                                                                                                      tAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGt                                               <  1:2887837/150‑1 (MQ=255)
                                                                                                       aaaCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGAGAAACTGACCGATAAGCCGGGTTCTgac                                              >  2:986121/1‑148 (MQ=255)
                                                                                                         cccAGGCGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGt                                            <  1:519175/149‑1 (MQ=255)
                                                                                                         aCCAGGTGACACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGt                                            <  1:2532763/150‑1 (MQ=255)
                                                                                                         aCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGt                                            <  1:1257014/150‑1 (MQ=255)
                                                                                                            aGGAGAAACTGACCGATAAGCCGCTATCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCATTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGATTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGa                                         <  1:652454/150‑1 (MQ=255)
                                                                                                               tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAATAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAg                                      >  2:2371451/1‑150 (MQ=255)
                                                                                                               tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAATCCGGGGTCTGTCGTGGACAg                                      >  2:2667737/1‑150 (MQ=255)
                                                                                                               tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCATTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTAGTTGACAg                                      >  2:1905878/1‑150 (MQ=255)
                                                                                                                gAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAAGCAAAAAACCCCCGAAGCGGGTTTTTAAGTAAATCAGGTGAAACAGACCGATAAGCCGGGGTCTGTCGTGGACAgg                                     >  1:622865/1‑149 (MQ=14)
                                                                                                                caaaCGGACCGAGAAGCCGAATTCTTTTGGGTTTTTCGTCGTGGAAAGTCTGTCCTCTTTCTGGCCCTCCAAAAGCAAAAACCCGCCGAAGCGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGt                                     <  2:184286/149‑1 (MQ=17)
                                                                                                                     tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaaaa                                                                                                          <  1:2029431/76‑1 (MQ=34)
                                                                                                                     tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaaaa                                                                                                          >  2:2799129/1‑76 (MQ=34)
                                                                                                                     tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaaaa                                                                                                          <  1:2799129/76‑1 (MQ=34)
                                                                                                                       aCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAc                                                                 <  1:2345907/115‑1 (MQ=35)
                                                                                                                        ccGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATAATATTTCTGCCCCTCCAAAATCAAAAACCCGCCCAAGCGGGTTTTTAAGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGGCCGTCATTCATc                             >  1:158287/1‑150 (MQ=17)
                                                                                                                         cGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCATTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGTAACGTACCGATAAGCCGGGGTCTGTCGTGGACAGTCATTCATCt                            >  1:2781453/1‑150 (MQ=17)
                                                                                                                         cGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCt                            >  2:947218/1‑150 (MQ=255)
                                                                                                                         cGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCAACt                            >  2:2139907/1‑150 (MQ=255)
                                                                                                                         cGATAAGCCGCTTTCTTGTGGGTATAGTGTCGTGGACAGTAATTAATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAATCCGGGTTCTTTCGGGGACAGTCATTAATcg                            >  2:915853/1‑149 (MQ=14)
                                                                                                                           aTAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCgg                                                      <  1:1841037/122‑1 (MQ=35)
                                                                                                                           aTAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAg                          >  2:210789/1‑150 (MQ=255)
                                                                                                                             aaGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGc                        <  1:2625501/150‑1 (MQ=255)
                                                                                                                               gCCGCTTTCTTTTGTGTATAGTGTCGTGGACAGTCATTGATCTTTCTGCCCCTCCACAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTTAAGCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCa                      <  1:93407/150‑1 (MQ=17)
                                                                                                                                 cGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGc                    >  2:233982/1‑150 (MQ=255)
                                                                                                                                 cGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGGTCTGTCGTGGACCGTCATTCATCAAGGCCAGc                    >  2:2362920/1‑150 (MQ=255)
                                                                                                                                 cGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACAGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGc                    >  2:908072/1‑150 (MQ=255)
                                                                                                                                   ctttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCCGCaa                  >  2:857484/1‑150 (MQ=255)
                                                                                                                                    tttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAAt                 <  1:1319409/150‑1 (MQ=255)
                                                                                                                                       ctttTGTGTAGCGTGTCGTGGACTGTCACTCATCTGTCTGCCCCTCCACACGCAAAAACCCGCCGAAGAGGGTTTTTACGTAAATCAGGTTAAACGGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGc              <  2:268124/150‑1 (MQ=18)
                                                                                                                                        ttttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAAACAGGTGAAACTGACCGATAAGCCGGGTTCTTTCGTGGACAGTCATTAATATATGCCAGCAATAGCt             >  1:5756/1‑150 (MQ=17)
                                                                                                                                        ttttGGGTATAGCGTCGTGGACAGTCATTAATCATTCTGCCCCTCCAAAAGCAAAAACCCCCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCAGTCCTGGACAGTCATTCATCTAGGCCAGCAATCGCt             >  2:2208591/1‑150 (MQ=18)
                                                                                                                                         tttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTc            >  2:1118647/1‑150 (MQ=255)
                                                                                                                                         tttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACAGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCAACTAGGCCAGCAATCGCTc            >  2:2355247/1‑150 (MQ=255)
                                                                                                                                         tttGGGTATAGCGTCGTGGACAGTAATACATATTTCTGCCCCGCCAAAAGCAAAAACCCCCCGAAGAGGGTTTTTACGTAAATCAGGTGAAAATGACCGATAAGCCGGGTTCTGTCGTGGACATTAATTCATCTAGGCCAGCAAACGCTc            >  2:1118471/1‑150 (MQ=255)
                                                                                                                                           tGGGTAGAGCGGCGGGGACCGTCATTCATCTTTCTGCCCCTCCAAACGCAAAAACCCGCCGCAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCAc          <  2:2077896/150‑1 (MQ=21)
                                                                                                                                               tataGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGc      <  2:1528391/150‑1 (MQ=255)
                                                                                                                                                ataGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCt     <  2:483795/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAA  >  NC_000913/3269995‑3270291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: